Chen J.,Suzhou Hospital of Traditional Chinese Medicine |
Zhu B.,Jiangsu Hospital of Traditional Chinese Medicine |
Li Y.,Suzhou Hospital of Traditional Chinese Medicine
DNA and Cell Biology | Year: 2013
The tumor suppressor P53 and its negative regulator mouse double minute 2 (MDM2) play crucial roles in carcinogenesis. Previous case-control studies also revealed that P53 72Arg>Pro and MDM2 309T>G polymorphisms contribute to the risk of common cancers. However, the relationship between these two functional polymorphisms and adult acute lymphoblastic leukemia (ALL) susceptibility has not been explored. In this study, we performed a case-control study to explore the association between MDM2 and P53 gene polymorphisms and ALL risk in a Chinese population. We found an increased adult ALL risk associated with the MDM2 GG (odds ratio [OR]=2.79, 95% confidence interval [95% CI]=1.67-4.68) and TG (OR=1.49, 95% CI=0.95-2.53) genotypes. An increased risk associated with the P53 Pro/Pro genotype (OR=2.22, 95% CI=1.30-3.79) compared with the Arg/Arg genotype was also observed. Furthermore, the gene-gene interaction of MDM2 and P53 polymorphisms increased the adult ALL risk in a super-multiplicative manner (OR for the presence of both MDM2 GG and P53 Pro/Pro genotypes=8.05, 95% CI=2.53-25.58). These findings suggest that polymorphisms of MDM2 and P53 genes may be genetic modifiers for developing adult ALL. © 2013 Mary Ann Liebert, Inc.
Liu J.,Suzhou Hospital of Traditional Chinese Medicine |
Li X.,Shanghai University of Traditional Chinese Medicine |
Li X.,Suzhou Hospital of Traditional Chinese Medicine |
Tang D.,Shanghai University of Traditional Chinese Medicine |
And 7 more authors.
Pain Physician | Year: 2013
Background: Osteoporotic vertebral compression fractures (OVCFs) are the most common osteoporotic fractures. Pain is the main symptom. Percutaneous vertebroplasty (PVP) is a therapeutic procedure performed to reduce pain in vertebral compression fractures. Numerous case series and several small, non-blinded, non-randomized controlled studies have suggested that vertebroplasty is an effective means of relieving pain from osteoporotic fractures. However, a recent pooled analysis from 2 multicenter randomized controlled trials concluded that the improvement in pain afforded by PVP was similar to placebo. Objective: To compare the amount of pain reduction measured using the visual analog scale when OVCF is treated with vertebroplasty or conservatively, and assess the clinical utility of PVP. Design: A meta-analysis and systematic review of randomized controlled trials was performed comparing pain reduction following vertebroplasty and conservative treatment. Limitations: There were few data sources from which to extract abstracted data or published studies. There were only 5 randomized controlled trials that met our criteria. The conservative treatments used as comparators in these trials were different. Methods: A search of MEDLINE from January 1980 to July 2012 using PubMed, the Cochrane Database of Systematic Reviews and Controlled Trials, CINAHL, and EMBASE. Relevant reports were examined by 2 independent reviewers and the references from these reports were searched for additional trials, using the criteria established in the QUOROM statement. Results: Pooled results from 5 randomized controlled trials are shown. There was no difference in pain relief in the PVP group at 2 weeks and one month when compared with the conservatively managed group. Pain relief in the PVP group was greater than that of the conservative group at 3 months, 6 months, and 12 months. However, after subgroup analysis, pain scores were similar between the PVP group and the sham injection group from 2 weeks to 6 months. Compared with non-operative therapy, PVP reduced pain at all times studied. Conclusion: PVP has some value for relieving pain; however, the possibility of a placebo effect should be considered. PVP has gained acceptance as a complementary treatment when conservative management has failed before its benefits have been fully understood. More large scale, double blinded, controlled trials are necessary in order to quantify the pain relief afforded by PVP more precisely.
Yao W.-J.,Nanjing University |
Ouyang B.-S.,Suzhou Hospital of Traditional Chinese Medicine
Journal of Acupuncture and Tuina Science | Year: 2014
Objective: To observe the effect of relaxing needling plus rehabilitation training on post-stroke upper limb dysfunction. Methods: Sixty-eight eligible subjects with post-stoke upper limb dysfunction were randomized into 2 groups by the random number table, 34 in each group, to respectively receive relaxing needling method or ordinary needling method in addition to rehabilitation training for 2 months. Fugl-Meyer assessment of upper limb motor function, neurological defect score, and clinical efficacies were observed and compared. Results: The two groups both showed effects. The total effective rate in the relaxing needling group was 93.8% versus 84.9% in the ordinary needling group, and the difference was statistically significant (P<0.05). The relaxing needling group was better than the ordinary needling group in improving the motor function of upper limb and neurological defect (P<0.05). Conclusion: Relaxing needling plus rehabilitation training can significantly improve the post-stroke motor dysfunction of upper limb, better than ordinary needling plus rehabilitation training. © 2014 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.
Zhang L.,Soochow University of China |
Zhang L.,Suzhou Hospital of Traditional Chinese Medicine |
Jiang G.,Suzhou Hospital of Traditional Chinese Medicine |
Yao F.,Suzhou Hospital of Traditional Chinese Medicine |
And 7 more authors.
PLoS ONE | Year: 2012
Background: Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed tumors worldwide and is known to be resistant to conventional chemotherapy. New therapeutic strategies are urgently needed for treating HCC. Osthole, a natural coumarin derivative, has been shown to have anti-tumor activity. However, the effects of osthole on HCC have not yet been reported. Methods and Findings: HCC cell lines were treated with osthole at various concentrations for 24, 48 and 72 hours. The proliferations of the HCC cells were measured by MTT assays. Cell cycle distribution and apoptosis were determined by flow cytometry. HCC tumor models were established in mice by subcutaneously injection of SMMC-7721 or Hepa1-6 cells and the effect of osthole on tumor growths in vivo and the drug toxicity were studied. NF-κB activity after osthole treatment was determined by electrophoretic mobility shift assays and the expression of caspase-3 was measured by western blotting. The expression levels of other apoptosis-related genes were also determined by real-time PCR (PCR array) assays. Osthole displayed a dose- and time-dependent inhibition of the HCC cell proliferations in vitro. It also induced apoptosis and caused cell accumulation in G2 phase. Osthole could significantly suppress HCC tumor growth in vivo with no toxicity at the dose we used. NF-κB activity was significantly suppressed by osthole at the dose- and time-dependent manner. The cleaved caspase-3 was also increased by osthole treatment. The expression levels of some apoptosis-related genes that belong to TNF ligand family, TNF receptor family, Bcl-2 family, caspase family, TRAF family, death domain family, CIDE domain and death effector domain family and CARD family were all increased with osthole treatment. Conclusion: Osthole could significantly inhibit HCC growth in vitro and in vivo through cell cycle arrest and inducing apoptosis by suppressing NF-κB activity and promoting the expressions of apoptosis-related genes. © 2012 Zhang et al.
Wu J.,Suzhou Hospital of Traditional Chinese Medicine
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013
To investigate the intervention effects and mechanisms of the compound panax notoginsenoside granules (CPNG) on bleomycin-induced pulmonary fibrosis in rats. Sixty male Sprague-Dawley rats were randomly divided into 6 groups, including normal control group, model group, prednisolone group, and CPNG (high, middle and low-dose) groups. Each group included 10 Sprague-Dawley rats. Except the control group, pulmonary fibrosis model rats were injected with bleomycin (5 mg/kg) via trachea. On the second day of the bleomycin injection, the rats in each treatment group were given prednisolone (3.33 mg/kg) and corresponding doses of CPNG (100, 50, 25 mg/kg) by nasogastric feeding daily; the normal control group and the model group were given the equal volume distilled water daily. Twenty-eight days later, all rats were executed and the lung tissues were subjected to the histopathological examination via HE and Masson staining. The hydroxyproline (HYP) in lung tissues was assessed by alkaline hydrolysis. The expressions of TGF-β1, Smad2, and Smad7 proteins were analyzed by Western blotting. Compared with the model group, the histopathological results revealed that CPNG dramatically decreased the severity of lung inflammation and fibrosis in rats (P<0.01). Compared with the control group, the HYP in lung tissues was reduced in all of the CPNG groups (P<0.01). Moreover, CPNG inhibited the expressions of TGF-β1 or Smad2 proteins (P<0.01), but enhanced the expression of Smad7 protein in the rat lung tissues (P<0.01). CPNG has the prevention and treatment effect on the bleomycin-induced pulmonary fibrosis in rats, and it might be attributed to the regulation on the TGF-β1/Smads signaling pathway.
PubMed | Suzhou Hospital of Traditional Chinese Medicine and Shanghai University of Traditional Chinese Medicine
Type: Journal Article | Journal: Pain physician | Year: 2017
Lumbar disc herniation (LDH), a common disease, is often treated conservatively, frequently resulting in spontaneous resorption of the herniated disc. The incidence of this phenomenon, however, remains unknown.To analyze the incidence of spontaneous resorption after conservative treatment of LDH using computed tomography and magnetic resonance imaging.Meta-analysis and systematic review of cohort studies.The work was performed at The Suzhou Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine.We initiated a search for the period from January 1990 to December 2015 using PubMed, Embase, and the Cochrane Library. Two independent reviewers examined the relevant reports. The references from these reports were also searched for additional trials using the criteria established in the PRISMA statement.Our results represent the pooled results from 11 cohort studies. The overall incidence of spontaneous resorption after LDH was 66.66% (95% CI 51% - 69%). The incidence in the United Kingdom was 82.94% (95% CI 63.77% - 102.11%). The incidence in Japan was 62.58% (95% CI 55.71% - 69.46%).Our study was limited because there were few sources from which to extract data, either in abstracts or published studies. There were no randomized, controlled trials that met our criteria.The phenomenon of LDH reabsorption is well recognized. Because its overall incidence is now 66.66% according to our results, conservative treatment may become the first choice of treatment for LDH. More large-scale, double-blinded, randomized, controlled trials are necessary to study the phenomenon of spontaneous resorption of LDH.Key words: Lumbar, disc herniation, spontaneous resorption, conservative treatment, incidence, country, meta-analysis, systematic review, observational studies, study designs.
PubMed | Suzhou Hospital of Traditional Chinese Medicine, East China University of Science and Technology and Shanghai University of Traditional Chinese Medicine
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016
The present study was performed to investigate the role of p38 mitogenactivated protein kinase (MAPK) in the resorption of herniated intervertebral discs in 30rats. In the noncontained and p38 MAPK inhibition (p38i) groups, two coccygeal intervertebral discs (IVDs) were removed and wounded prior to relocation into the subcutaneous space of the skin of the back. In the contained group, the cartilage endplates maintained their integrity. Furthermore, SB203580 was injected intraperitoneally into the p38i group, whereas saline was injected into the other two groups. In the noncontained group, the weight of the relocated IVDs decreased to a greater extent over time when compared with the contained and p38i groups. Phosphorylated p38, tumor necrosis factor, and interleukin1 were observed to exhibit higher expression levels in the noncontained group compared with the contained and p38i groups, at weeks1 and4 postsurgery. The expression level of caspase3 and the densities of apoptotic disc cells were significantly higher in the noncontained group compared with the contained and p38i groups at 4weeks postsurgery. In conclusion, p38 MAPK induces apoptosis in IVDs, while also accelerating the resorption of the relocated IVDs. Thus, p38 MAPK may be important in spontaneous resorption of IVDs.
PubMed | Suzhou Hospital of Traditional Chinese Medicine, Nanjing University and Yancheng Hospital of Traditional Chinese Medicine
Type: Journal Article | Journal: The journal of obstetrics and gynaecology research | Year: 2016
We aimed to evaluate the preventive effect of the ligustrazine nanoparticles nano spray (LNNS) for postoperative peritoneal adhesions in female rat models.Fifty Wistar female rats weighting 250-300g were randomly assigned to seven equal groups. All animals in the seven groups underwent midline laparotomy and ceca were abraded with sterile rasp. Group 1 underwent sham operations without treatment. In group 2, a postoperative peritoneal adhesion model was created, but no medication was given. In group 3, a postoperative peritoneal adhesion model was treated with LNNS, 2.5mg/kg. In group 4, a postoperative peritoneal adhesion model was treated with LNNS, 5mg/kg. In group 5, a postoperative peritoneal adhesion model was treated with LNNS, 10mg/kg. In group 6, a postoperative peritoneal adhesion model was treated with polylactic acid (PLA) nanoparticle. In group 7, a postoperative peritoneal adhesion model was treated with ligustrazine, 2.5mg/kg. Ten days after surgery, macroscopic and pathologic assessments were performed, and peritoneal fluid samples were collected in each group. The levels of tumor necrosis factor-, tissue plasminogen activator and plasminogen activator inhibitor-1 in peritoneal fluid were determined by enzyme-linked immunosorbent assay.The adhesion score and extent of groups 4 and 5 was lower than that of group 2 in macroscopic assessment (P < 0.05). A comparison of tumor necrosis factor- and tissue plasminogen activator level in the peritoneal fluid also demonstrated significant differences among groups 2, 4 and 5 (P < 0.05). The levels of plasminogen activator inhibitor-1 in peritoneal fluid in the LNNS groups were decreased compared to group 1.We suggest that LNNS could reduce peritoneal adhesion formation and it could be applied as a novel intervention for postoperative peritoneal adhesion.
PubMed | Suzhou Hospital of Traditional Chinese Medicine and Soochow University of China
Type: Journal Article | Journal: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association | Year: 2016
We investigated whether admission serum calcium and phosphate levels are associated with short- and long-term outcomes in patients with acute intracerebral hemorrhage.A total of 365 patients with acute intracerebral hemorrhage were enrolled in this study. Participants were classified into 4 subgroups according to serum calcium or phosphate quartiles. Demographic characteristics, lifestyle risk factors, medical history, and other clinical characteristics were recorded for all the participants. Excellent outcome was defined as discharge or 3-month modified Rankin scale scores of 0-1.Univariate analysis comparing the highest and lowest quartiles indicated that an elevated calcium level was associated with 2.26- and 2.28-fold increases in the odds for discharge and 3-month excellent outcome, respectively. After adjustment for age, sex, and other potential risk factors, patients in the highest quartile still had significantly increased odds of discharge and 3-month excellent outcome; the corresponding odds ratios (ORs) were 3.43 (95% confidence interval [[CI], 1.03-11.44) and 5.36 (95% CI, 1.69-16.98). When calcium was divided into two groups, the ORs of higher calcium were 2.9 (95% CI, 1.1-7.62) and 2.8 (95% CI, 1.15-6.82) for discharge and 3-month excellent outcome, respectively. However, no significant association was observed between serum phosphate and excellent outcome.Elevated admission serum calcium level but not phosphate level is positively associated with excellent outcome at discharge or 3 months in acute intracerebral hemorrhage patients.
PubMed | Suzhou Hospital of Traditional Chinese Medicine, Zhejiang University, Childrens Hospital of Soochow University and Soochow University of China
Type: | Journal: Free radical biology & medicine | Year: 2017
Our previous study has reported that the pentose phosphate pathway product nicotinamide adenine dinucleotide phosphate (NADPH) protected neurons against ischemia/reperfusion-induced brain injury. NADPH can either act as a co-enzyme to produce GSH or a substrate of NADPH oxidase (NOX) to generate ROS. This study was designed to elucidate the effects of co-treatment with NADPH and NOX inhibitor apocynin on ischemia/reperfusion-induced brain inflammation and neuronal injury. The results showed that both NADPH and apocynin markedly attenuated ischemia/reperfusion-induced increases in the levels of NOX2, NOX4 and ROS. NADPH and apocynin significantly inhibited the phosphorylation and degradation of IB, NF-Bp65 nuclear localization, and the expression of NF-B target gene cyclooxygenase (COX2) and inducible nitric oxide synthase (iNOS). Furthermore, both NADPH and apocynin suppressed the expression of inflammasome proteins including NLRP3 ASC, caspase-1, interleukin (IL)-1 and IL-18 in the ischemic cortex as revealed by Western blot analysis and immunofluorescence. Moreover, all these effects were greatly amplified by combination of NADPH and apocynin. Both NADPH and apocynin significantly reduced infarct volume, improved post-stroke survival, and recovery of neurological functions in mouse model of stroke. Consistently, the combination of NADPH and apocynin produced greater beneficial effects in against ischemic brain damage. These studies suggest that, beyond anti-oxidative effects, NADPH may also have anti-inflammatory effects and combination of NADPH and NOX inhibitors could produce a greater neuroprotective effect in ischemic stroke.