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Jiang L.,Nanjing Agricultural University | Zhang L.,Nanjing Agricultural University | Zhang L.,Suzhou Academy of Agricultural Science | Shi Y.,Nanjing Agricultural University | And 2 more authors.
Journal of Proteomics | Year: 2014

Regulation of peach fruit ripening by heat combined with 1-Methylcyclopropene (1-MCP) was studied by 2-dimensional gel electrophoresis (2-DE) and Matrix-Assisted Laser Desorption/Ionization Time of Flight tandem Mass Spectrometry (MALDI-TOF/TOF). Proteins from peach fruits after harvest (CK) and treated by heat combined with 1-MCP (HM) were then stored at room temperature for 0, 1, 3 and 5. days. Among the identified 42 protein spots, the differential abundant proteins belonged to pathways of defense and response (35.71%), energy and metabolism (30.95%), ripening and senescence (14.29%), cell structure (14.29%) and protein fate (4.76%). Compared with separate heat or 1-MCP treatment, pectinesterase inhibitor (PEI) and heat shock protein (HSP) appeared, and abscisic stress ripening-like protein (ASR) disappeared after the treatment, while HM specifically increased the abundances of glutathione peroxidase (GPX), peroxiredoxin, calmodulin, and decreased those of cytosolic malate dehydrogenase, d-3-phosphoglycerate dehydrogenase (GAPDH) and glutamine synthetase. HM treatment protected fruit cells by enhancing the capabilities of stress response and defense, inhibiting substance and energy metabolism, limiting cell calcium loss. The results suggest that the self-defense capability of peach fruit was boosted by HM treatment. This study is informative in exploring the influences of HM on peach fruit ripening by demonstrating that 1-MCP and heat functioned synergistically. Biological significance: To analyze the functions of differentially expressed proteins and to elucidate the response of early-maturing melting peach fruit (cv. Huiyulu) during ripening, we herein, for the first time, studied the effects of HM treatment on involved protein profiles by a proteomic approach with 2-DE and MALDI-TOF/TOF. This study successfully verified that HM functioned synergistically rather than simply superimposed on the proteome level.In addition, this study explains the molecular mechanism regarding peach fruit development and ripening on the proteome level, offers new insights into relevant physiological mechanism, and provides theoretical evidence for reinforcing quality control of post-harvest peach fruit in practice. © 2013 Elsevier B.V. Source

Jiang L.,Nanjing Agricultural University | Kang R.,Nanjing Agricultural University | Zhang L.,Suzhou Academy of Agricultural Science | Jiang J.,Qufu Normal University | Yu Z.,Nanjing Agricultural University
Food Chemistry | Year: 2015

Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7 days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P < 0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. © 2014 Published by Elsevier Ltd. Source

Deng J.,Jiangsu Academy of Agricultural Sciences | Deng J.,Suzhou Academy of Agricultural Science | Li S.,Jiangsu Academy of Agricultural Sciences | Hong J.,Zhejiang University | And 2 more authors.
Virology Journal | Year: 2013

Background: Rice stripe virus (RSV), which is transmitted by small brown planthopper (Laodelphax striatellus Fallén, SBPH), has been reported to be epidemic and cause severe rice stripe disease in rice fields in many East Asian countries, including China. Investigation on viral localization in the vector is very important for elucidating transmission mechanisms of RSV by SBPH. In this study, transmission electron microscopy and immuno-gold labeling technique were used to investigate the subcellular localization of the ribonucleoproteins (RNPs) of RSV in the digestive tract, muscles, ovary and testes of SBPH. Results: A lot of amorphous RSV inclusion bodies with high electron density were observed in the cytoplasmic matrix and vacuoles of follicular cells of ovarioles in viruliferous SBPH, which were very similar to viral inclusions formed in rice cells. After magnified, it was found that sand-like or parallel filamentary structures were constructed inside the electron-dense inclusions. A large numbers of RSV RNPs distributed diffusely throughout the eggshell surface and interior of ovum, midgut lumen and epithelial cells, while the amount of the virus in muscles was far less than that in the ovary and midgut tissues. Besides RSV, numerous endogenous microorganisms were also observed in SBPH body, including yeast-like endosymbiotes (YLES), endosymbiotic bacteria and insect virus. Conclusions: According to the results of the virus localization, a potential mechanism of RSV transovarial transmission was proposed that RSV might replicate and accumulate initially in the inclusions of follicular cells, then exploit the pathway of the nutrition transportation to pass through the eggshell and spread into the oocytes along with the nutrition. Moreover, RSV might exploit muscles for its spread in vector body with a lower efficiency. © 2013 Deng et al.; licensee BioMed Central Ltd. Source

Yang H.,Nanjing Agricultural University | Feng J.,Nanjing Agricultural University | Zhai S.,Nanjing Agricultural University | Dai Y.,Nanjing Agricultural University | And 6 more authors.
Soil and Tillage Research | Year: 2016

As a novel soil tillage practice, ditch-buried straw return (DB-SR) has exhibited positive effects on soil carbon sequestration, nitrogen retention and rice yield in previous studies. However, little is known about how long-term DB-SR affects soil hydrothermal and microbial processes. Our objective is to test whether DB-SR will alter the soil water potential, temperature and microbial community in a wheat field following rice cultivation. In this study, we found significant alterations in soil water potential, temperature, and microbial communities driven by DB-SR. On average, soil water potential was significantly reduced by 37.33% and 17.56% under DB-SR to a depth of 20 cm (DB-SR-20) and 40 cm (DB-SR-40), respectively. DB-SR-20 increased soil mean daily temperature and daily range of temperature more than DB-SR-40, possibly caused by decreased water content, especially at soil depths of 10 and 15 cm. Both DB-SR-20 and DB-SR-40 led to distinct shifts in soil bacterial and fungal community composition. DB-SR-20 significantly increased the activities of peroxidase, cellobiohydrolase, urease, and acid phosphatase by 3.5%, 75.0%, 81.4% and 41.7%, respectively, but had no effects on β-. d-glucosidase activity. DB-SR-40, in contrast, significantly increased the activities of peroxidase and cellobiohydrolase by 2.4% and 36.0%, respectively, but showed no effects on urease and acid phosphatase. It did, however, reduce β-. d-glucosidase activity by 15.0%. Overall functional diversity was increased by 29.9% under DB-SR-20 but was not affected by DB-SR-40. Our results suggest that these improvements in soil ecological processes driven by DB-SR will promote wheat yield in a rice-wheat rotation system. © 2016 Elsevier B.V. Source

Li J.,Suzhou Academy of Agricultural Science | Li J.,Suzhou Administrative Institution | Jiang H.,Suzhou Academy of Agricultural Science | Bai Y.,Shanghai Academy of Environmental Science | And 4 more authors.
Ecological Indicators | Year: 2016

With the development of ecological science, the demand to integrate ecosystem services into ecological management is increasing. Stakeholders are interested in comparing stocks and ability to supply ecosystem services in different regions. However, different areas may have different primary ecosystem services and knowledge of the aggregated value of ecosystem services may be lacking, making comparisons difficult. Relevant indicators that can integrate a group of ecosystem services for comparison are therefore needed. This paper formulated two indicators, ecosystem service supply rate and supply-demand ratio, and applied them in a case study to compare and map the spatial-temporal status of ecosystem services and the ability to supply these in different regions. Using nine regions in the Taihu River Basin in China as an example, data obtained from high-resolution spatial land use, land cover maps and stakeholder interviews were used to identify the spatial-temporal potential supply and flow of ecosystem services and human demand patterns. The results showed that ecosystem service supply rate had declined from 2000 to 2010, indicating that the overall proportion of potential ecosystem services turned into flow in the Taihu River Basin declined. Determination of supply-demand ratio revealed that the status of ecosystem service provision in the basin is in deficit, with the deficit increasing between 2000 and 2010. These findings indicate that ecosystem service supply rate and supply-demand ratio are useful indicators of ecosystem service status and can permit comparisons between regions on both a spatial and temporal scale. © 2015 Elsevier Ltd. All rights reserved. Source

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