Zhang Y.-N.,Huaibei Normal University |
He P.,Guizhou University |
Xue J.-P.,Huaibei Normal University |
Guo Q.,Suzhou Academy of Agricultural science |
And 4 more authors.
Journal of Asia-Pacific Entomology | Year: 2017
The beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae), is a key pest of various agricultural crops in many countries throughout the world. The pest requires extensive use of pesticides and field-evolved resistances to conventional insecticides in China and other countries. Pinellia ternata Breit is native to the eastern part of Asia and found mainly in China, which has been used in traditional Chinese medicines for > 1000 years, but few studies have focused on the insecticidal activity of the P. ternate. In order to find natural products that could be used to control the pest in an safe, efficient and ecofriendly manner, we first time analyzed the components of the anhydrous ethanolic extracts from the tubers of Pinellia ternate by using GC–MS method, and then investigated the insecticidal activities and biochemical mechanisms of the extracts against S. exigua. The result of GC–MS showed that 2-Methoxy-4-vinylphenol may be an insecticidal active component, and we also found the extracts had notable insecticidal activity and disturbed the regular metabolism of S. exigua mainly through altering the activities of detoxification enzymes, digestive enzymes and protective enzymes. These properties suggest that the anhydrous ethanolic extracts from P. ternate can serve as a potential, efficient and ecofriendly S. exigua-control biopesticide. © 2017
Li J.,Suzhou Academy of Agricultural science |
Li J.,Suzhou Administrative Institution |
Jiang H.,Suzhou Academy of Agricultural science |
Bai Y.,Shanghai Academy of Environmental science |
And 4 more authors.
Ecological Indicators | Year: 2016
With the development of ecological science, the demand to integrate ecosystem services into ecological management is increasing. Stakeholders are interested in comparing stocks and ability to supply ecosystem services in different regions. However, different areas may have different primary ecosystem services and knowledge of the aggregated value of ecosystem services may be lacking, making comparisons difficult. Relevant indicators that can integrate a group of ecosystem services for comparison are therefore needed. This paper formulated two indicators, ecosystem service supply rate and supply-demand ratio, and applied them in a case study to compare and map the spatial-temporal status of ecosystem services and the ability to supply these in different regions. Using nine regions in the Taihu River Basin in China as an example, data obtained from high-resolution spatial land use, land cover maps and stakeholder interviews were used to identify the spatial-temporal potential supply and flow of ecosystem services and human demand patterns. The results showed that ecosystem service supply rate had declined from 2000 to 2010, indicating that the overall proportion of potential ecosystem services turned into flow in the Taihu River Basin declined. Determination of supply-demand ratio revealed that the status of ecosystem service provision in the basin is in deficit, with the deficit increasing between 2000 and 2010. These findings indicate that ecosystem service supply rate and supply-demand ratio are useful indicators of ecosystem service status and can permit comparisons between regions on both a spatial and temporal scale. © 2015 Elsevier Ltd. All rights reserved.
Comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior spikelets to drought stress using isobaric tags for relative and absolute quantification
Dong M.,Suzhou Academy of Agricultural Science |
Dong M.,Yangzhou University |
Gu J.,Suzhou Academy of Agricultural Science |
Gu J.,Yangzhou University |
And 7 more authors.
Journal of Proteomics | Year: 2014
The biological functions of the differentially abundant proteins between superior and inferior spikelet grains were investigated based on the isobaric tags for relative and absolute quantification to further clarify the mechanism of rice grain filling at the proteomic level, as well as the response of inferior spikelets to drought dress (-. 20. kPa or -. 40. kPa). Compared with superior spikelets, inferior ones had lower sink strength due to the lower sink activities (lower abundances of ADP-glucose pyrophosphorylase, granule-bound starch synthase, starch branching enzyme and pullulanase) and smaller sink sizes (lower abundances of structural proteins). The slower and later grain filling resulted from the weaker decomposition and conversion of photoassimilate and the slower cell division. Moderate drought stress (-. 20. kPa) promoted the grain filling of inferior spikelets through regulating the proteins associated with photoassimilate supply and conversion. These proteins may be important targets for rice breeding programs that raise the rice yield under drought condition. The findings offer new insights into rice grain-filling and provide theoretical evidences for better quality control and scientific improvement of super rice in practice. Biological significance: Rice cultivars with large panicles do not always guarantee high yield and grain quality probably due to the slow grain filling and many unfilled grains of inferior spikelets. In general, earlier-flowering superior spikelets, which are usually located on apical primary branches, fill faster and produce larger and heavier grains. In contrast, later-flowering inferior spikelets located on proximal secondary branches are either sterile or fill slowly and poorly, and the differences are more significant in large panicle rice or super rice. The increase of rice yield has been limited by the unsatisfactory grain filling of inferior spikelets, and the inferior spikelets are more prone to environmental factors during grain filling. Thus, we herein investigated the biological functions of differently abundant proteins between superior and inferior spikelet grains by using iTRAQ to unravel the mechanism of rice grain filling and the response of inferior spikelets to drought stress at proteomic level. This study offers new insights into rice grain-filling and provides valuable evidences for better quality control and scientific improvement of super rice in practice. © 2014 Elsevier B.V.
Jiang L.,Nanjing Agricultural University |
Zhang L.,Nanjing Agricultural University |
Zhang L.,Suzhou Academy of Agricultural Science |
Shi Y.,Nanjing Agricultural University |
And 2 more authors.
Journal of Proteomics | Year: 2014
Regulation of peach fruit ripening by heat combined with 1-Methylcyclopropene (1-MCP) was studied by 2-dimensional gel electrophoresis (2-DE) and Matrix-Assisted Laser Desorption/Ionization Time of Flight tandem Mass Spectrometry (MALDI-TOF/TOF). Proteins from peach fruits after harvest (CK) and treated by heat combined with 1-MCP (HM) were then stored at room temperature for 0, 1, 3 and 5. days. Among the identified 42 protein spots, the differential abundant proteins belonged to pathways of defense and response (35.71%), energy and metabolism (30.95%), ripening and senescence (14.29%), cell structure (14.29%) and protein fate (4.76%). Compared with separate heat or 1-MCP treatment, pectinesterase inhibitor (PEI) and heat shock protein (HSP) appeared, and abscisic stress ripening-like protein (ASR) disappeared after the treatment, while HM specifically increased the abundances of glutathione peroxidase (GPX), peroxiredoxin, calmodulin, and decreased those of cytosolic malate dehydrogenase, d-3-phosphoglycerate dehydrogenase (GAPDH) and glutamine synthetase. HM treatment protected fruit cells by enhancing the capabilities of stress response and defense, inhibiting substance and energy metabolism, limiting cell calcium loss. The results suggest that the self-defense capability of peach fruit was boosted by HM treatment. This study is informative in exploring the influences of HM on peach fruit ripening by demonstrating that 1-MCP and heat functioned synergistically. Biological significance: To analyze the functions of differentially expressed proteins and to elucidate the response of early-maturing melting peach fruit (cv. Huiyulu) during ripening, we herein, for the first time, studied the effects of HM treatment on involved protein profiles by a proteomic approach with 2-DE and MALDI-TOF/TOF. This study successfully verified that HM functioned synergistically rather than simply superimposed on the proteome level.In addition, this study explains the molecular mechanism regarding peach fruit development and ripening on the proteome level, offers new insights into relevant physiological mechanism, and provides theoretical evidence for reinforcing quality control of post-harvest peach fruit in practice. © 2013 Elsevier B.V.
Jiang L.,Nanjing Agricultural University |
Kang R.,Nanjing Agricultural University |
Zhang L.,Suzhou Academy of Agricultural science |
Jiang J.,Qufu Normal University |
Yu Z.,Nanjing Agricultural University
Food Chemistry | Year: 2015
Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7 days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P < 0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. © 2014 Published by Elsevier Ltd.
Deng J.,Jiangsu Academy of Agricultural Sciences |
Deng J.,Suzhou Academy of Agricultural science |
Li S.,Jiangsu Academy of Agricultural Sciences |
Hong J.,Zhejiang University |
And 2 more authors.
Virology Journal | Year: 2013
Background: Rice stripe virus (RSV), which is transmitted by small brown planthopper (Laodelphax striatellus Fallén, SBPH), has been reported to be epidemic and cause severe rice stripe disease in rice fields in many East Asian countries, including China. Investigation on viral localization in the vector is very important for elucidating transmission mechanisms of RSV by SBPH. In this study, transmission electron microscopy and immuno-gold labeling technique were used to investigate the subcellular localization of the ribonucleoproteins (RNPs) of RSV in the digestive tract, muscles, ovary and testes of SBPH. Results: A lot of amorphous RSV inclusion bodies with high electron density were observed in the cytoplasmic matrix and vacuoles of follicular cells of ovarioles in viruliferous SBPH, which were very similar to viral inclusions formed in rice cells. After magnified, it was found that sand-like or parallel filamentary structures were constructed inside the electron-dense inclusions. A large numbers of RSV RNPs distributed diffusely throughout the eggshell surface and interior of ovum, midgut lumen and epithelial cells, while the amount of the virus in muscles was far less than that in the ovary and midgut tissues. Besides RSV, numerous endogenous microorganisms were also observed in SBPH body, including yeast-like endosymbiotes (YLES), endosymbiotic bacteria and insect virus. Conclusions: According to the results of the virus localization, a potential mechanism of RSV transovarial transmission was proposed that RSV might replicate and accumulate initially in the inclusions of follicular cells, then exploit the pathway of the nutrition transportation to pass through the eggshell and spread into the oocytes along with the nutrition. Moreover, RSV might exploit muscles for its spread in vector body with a lower efficiency. © 2013 Deng et al.; licensee BioMed Central Ltd.
Zhao D.,Soochow University of China |
Qiao Z.,Suzhou Academy of Agricultural science |
Cheng X.,Soochow University of China |
Wang J.,Suzhou Academy of Agricultural science |
And 2 more authors.
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2014
The maize Ac/Ds transposable elements are members of the hAT transposon superfamily, and have stable transpositional activity in transgenic rice plants. Ac/Ds transposable elements are considered to transpose via a conservative non-replicative "cut and paste" model, though their transposition mechanism is not completely understood. Previous studies have shown that Ds preferentially transposes to genetically linked sites after being excised from its original site in the presence of Ac-transposase. In this study, genomic sequences flanking Ds insertions from a Ds-tagged rice mutant and its rever- tant were determined by TAIL-PCR. The Ds insertion site, the excision footprint and the re-insertion sites in the mutant were identified using bioinformatics tool. The results showed that Ds element excised from its original insertion site on chromosome 3 by leaving an 8 bp footprint (CATCATGA), which resulted in exon changes in tagged gene. After the excision, Ds element was re-inserted into the coding sequences of two genes on chromosome 2 and chromosome 6, which encode a nicotianamine aminotransferase and a senescence-associated protein, respectively. The transposition behavior of Ds element in this study could not be fully explained by the "cut and paste" mechanism, while it is likely to transpose in a "cut and copy and paste" way.
Yang H.,Nanjing Agricultural University |
Feng J.,Nanjing Agricultural University |
Zhai S.,Nanjing Agricultural University |
Dai Y.,Nanjing Agricultural University |
And 6 more authors.
Soil and Tillage Research | Year: 2016
As a novel soil tillage practice, ditch-buried straw return (DB-SR) has exhibited positive effects on soil carbon sequestration, nitrogen retention and rice yield in previous studies. However, little is known about how long-term DB-SR affects soil hydrothermal and microbial processes. Our objective is to test whether DB-SR will alter the soil water potential, temperature and microbial community in a wheat field following rice cultivation. In this study, we found significant alterations in soil water potential, temperature, and microbial communities driven by DB-SR. On average, soil water potential was significantly reduced by 37.33% and 17.56% under DB-SR to a depth of 20 cm (DB-SR-20) and 40 cm (DB-SR-40), respectively. DB-SR-20 increased soil mean daily temperature and daily range of temperature more than DB-SR-40, possibly caused by decreased water content, especially at soil depths of 10 and 15 cm. Both DB-SR-20 and DB-SR-40 led to distinct shifts in soil bacterial and fungal community composition. DB-SR-20 significantly increased the activities of peroxidase, cellobiohydrolase, urease, and acid phosphatase by 3.5%, 75.0%, 81.4% and 41.7%, respectively, but had no effects on β-. d-glucosidase activity. DB-SR-40, in contrast, significantly increased the activities of peroxidase and cellobiohydrolase by 2.4% and 36.0%, respectively, but showed no effects on urease and acid phosphatase. It did, however, reduce β-. d-glucosidase activity by 15.0%. Overall functional diversity was increased by 29.9% under DB-SR-20 but was not affected by DB-SR-40. Our results suggest that these improvements in soil ecological processes driven by DB-SR will promote wheat yield in a rice-wheat rotation system. © 2016 Elsevier B.V.
Data in support of comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior spikelets to drought stress using isobaric tags for relative and absolute quantification
PubMed | Suzhou Academy of Agricultural Science
Type: | Journal: Data in brief | Year: 2015
We provide the raw data for protein and peptide identification and quantization of superior and inferior spikelets in hybrid rice during grain filling. The mass spectrometry proteomics data have been deposited to the Proteome Xchange Consortium via the PRIDE partner repository with the dataset identifier PXD001046. Our data presented here is also related to the article Comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior spikelets to drought stress using isobaric tags for relative and absolute quantification in the Journal of Proteomics .
PubMed | Suzhou Academy of Agricultural science and Soochow University of China
Type: Journal Article | Journal: Yi chuan = Hereditas | Year: 2014
The maize Ac/Ds transposable elements are members of the hAT transposon superfamily, and have stable transpositional activity in transgenic rice plants. Ac/Ds transposable elements are considered to transpose via a conservative non-replicative cut and paste model, though their transposition mechanism is not completely understood. Previous studies have shown that Ds preferentially transposes to genetically linked sites after being excised from its original site in the presence of Ac-transposase. In this study, genomic sequences flanking Ds insertions from a Ds-tagged rice mutant and its rever- tant were determined by TAIL-PCR. The Ds insertion site, the excision footprint and the re-insertion sites in the mutant were identified using bioinformatics tool. The results showed that Ds element excised from its original insertion site on chromosome 3 by leaving an 8 bp footprint (CATCATGA), which resulted in exon changes in tagged gene. After the excision, Ds element was re-inserted into the coding sequences of two genes on chromosome 2 and chromosome 6, which encode a nicotianamine aminotransferase and a senescence-associated protein, respectively. The transposition behavior of Ds element in this study could not be fully explained by the cut and paste mechanism, while it is likely to transpose in a cut and copy and paste way.