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Hwaseong, South Korea

Kang S.U.,Ajou University | Cho J.-H.,Suwon Catholic University | Chang J.W.,Ajou University | Shin Y.S.,Ajou University | And 7 more authors.
Cell Death and Disease | Year: 2014

Nonthermal plasma (NTP) is generated by ionization of neutral gas molecules, which results in a mixture of energy particles including electrons and ions. Recent progress in the understanding of NTP has led to its application in the treatment of various diseases, including cancer. However, the molecular mechanisms of NTP-induced cell death are unclear. The purpose of this study was to evaluate the molecular mechanism of NTP in the induction of apoptosis of head and neck cancer (HNC) cells. The effects of NTP on apoptosis were investigated using MTT, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling, Annexin V assays, and western blot analysis. The cells were examined for production of reactive oxygen species (ROS) using DCFCA or MitoSOX staining, intracellular signaling, and an animal model. NTP reduced HNC cell viability in a dosedependent manner and induced apoptosis. NTP resulted in alteration of mitochondrial membrane potential and accumulation of intracellular ROS generated from the mitochondria in HNC cells. Blockade of ROS production by N-acetyl-L-cysteine inhibited NTP-induced apoptosis. NTP led to the phosphorylation of c-JUN N-terminal kinase (JNK) and p38, but not extracellularregulated kinase. Treatment with JNK and p38 inhibitors alleviated NTP-induced apoptosis via ROS generation. Taken together, these results show that NTP induced apoptosis of HNC cells by a mechanism involving MAPK-dependent mitochondrial ROS. NTP inhibited the growth of pre-established FaDu tumors in a nude mouse xenograft model and resulted in accumulation of intracellular ROS. In conclusion, NTP induced apoptosis in HNC cells through a novel mechanism involving MAPK-mediated mitochondrial ROS. These findings show the therapeutic potential of NTP in HNC. © 2014 Macmillan Publishers Limited. Source


Chun Y.-M.,Suwon Catholic University | Cho S.-M.,The Korean Alcohol Research Foundation | Shin S.-M.,Handong Global University
Psychology of Addictive Behaviors | Year: 2010

The Stages of Change Readiness and Treatment Eagerness Scale (SOCRATES) is an instrument used to measure the level of motivation in regards to changing drinking and other addictive behaviors. While some initial factor analysis studies on the SOCRATES described a three-factor orthogonal structure of the scale, some other studies found a two-factor correlated structure. Therefore, the primary objective of the present study was to test the validity of the Korean language version of the instrument using a Korean population. The study examined the factor structure of the Korean version of the SOCRATES with clinical samples consisting of 219 inpatients and 271 outpatients with alcohol dependency. An exploratory factor analysis with an alpha factoring method revealed a three-factor correlated structure (i.e., Taking Steps, Recognition, and Ambivalence). The factorial structure of the SOCRATES Korean version corresponded almost exactly to that of its original French version as well as the German version. Moreover, confirmatory factor analyses showed that a three-factor correlated structure provided the best fit for the data. © 2010 American Psychological Association. Source


Shim H.J.,Eulji University | Jung H.,Kyung Hee University | Park D.C.,Suwon Catholic University | Lee J.H.,Kyung Hee University | Yeo S.G.,Kyung Hee University
Acta Oto-Laryngologica | Year: 2011

Conclusions: Ramsay Hunt syndrome (RHS) with multiple involvement of cranial nerves is more severe and intractable than RHS without such involvement. Objectives: Typically, RHS involves VII and VIII nerves and unilaterally, and RHS accompanied by multiple cranial neuropathy is very rare. We describe 11 patients who developed RHS with multicranial nerve involvement and we analyzed their clinical characteristics and compared them with those of patients with RHS not accompanied by multiple cranial neuropathy. Methods: During the period 19952009, we treated 339 patients with RHS; of these, 11 patients had concurrent multiple cranial neuropathy. We assessed the clinical characteristics of RHS patients with and without multiple cranial neuropathy. Results: The mean age of the 11 patients with multiple cranial neuropathy (6 men, 5 women) was 49.2 ± 19.4 years, although 7 were aged 50 years or older. Eight patients had right-sided and three had left-sided facial paralysis. The initial degree of facial paralysis was House-Brackmann (HB) grade IV in four patients (36.4%) and HB grade V in seven (63.6%). Six patients showed improvement in symptoms, whereas five (45.6%) showed no improvement. The recovery rates from facial paralysis in patients with and without multiple cranial neuropathy were 54.5% and 82.9%, respectively, and the complete recovery rates were 27.3% and 67.7%, respectively. © 2011 Informa Healthcare. Source


Shin I.H.,Kyung Hee University | Park D.C.,Suwon Catholic University | Kwon C.,Kyung Hee University | Yeo S.G.,Kyung Hee University
Archives of Otolaryngology - Head and Neck Surgery | Year: 2011

Objective: To evaluate changes in taste threshold in patients with chronic otitis media with effusion (COME) and their relationship with body mass index. A relationship has been suggested between pediatric obesity and COME, and we hypothesized that changes in taste function may occur in children with COME and that such changes may be associated with changes in body weight. Design: A prospective, nonrandomized, case-control study. Setting: A university tertiary care center. Subjects: The experimental group comprised 42 children with COME who underwent tympanostomy tube insertion, and the control group, 42 children without otitis media with effusion. Patients were enrolled between September 2007 and August 2009. Main Outcome Measure: Taste threshold was measured by electrogustometry, and 4 standard taste solutions (sucrose, sodium chloride, citric acid, and quinine hydrochloride) were used in chemical taste tests. Results: Body mass index was significantly higher in the COME than in the control group (P=.02). Electrogustometry showed that the anterior part of the tongue had a significantly higher taste threshold in the COME than in the control group (anterior right, P=.03; anterior left, P=.04), and chemical taste test results showed that sweet and salty tastes were significantly lower in the COME group (sweet, P=.02; salty, P=.04). Conclusion: These results showed that COME can cause changes in taste and that these changes may be related to pediatric obesity. ©2011 American Medical Association. All rights reserved. Source


Jo K.-W.,Catholic University of Korea | Park I.-S.,Catholic University of Korea | Hong J.T.,Suwon Catholic University
Journal of Clinical Neuroscience | Year: 2011

The treatment of C1 Jefferson fractures is controversial. Non-surgical treatment with halo fixation always bears the risk of insufficient healing with further instability and increasing neck pain. However, a C1-2 fusion can markedly decrease the rotatory motion of the neck. The aim of this report is to describe a new treatment for C1 Jefferson fractures. We used open reduction and C1 fixation using a bilateral C1 lateral mass screw construct. The screws were connected with a rod and nuts to reduce lateral spread of the lateral masses. This mehod is an alternative surgical option for C1 Jefferson fractures in select patients and can maintain important C1-2 joint motion. © 2010 Elsevier Ltd. All rights reserved. Source

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