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Baleeiro R.B.,Charite - Medical University of Berlin | Baleeiro R.B.,University of Sao Paulo | Wiesmuller K.-H.,University of Tubingen | Wiesmuller K.-H.,EMC Microcollections GmbH | And 4 more authors.
PLoS ONE | Year: 2013

Dendritic cells (DCs) are key activators of cellular immune responses through their capacity to induce naïve T cells and sustained effector T cell responses. This capacity is a function of their superior efficiency of antigen presentation via MHC class I and class II molecules, and the expression of co-stimulatory cell surface molecules and cytokines. Maturation of DCs is induced by microbial factors via pattern recognition receptors such as Toll-like receptors, pro-inflammatory cytokines or cognate interaction with CD4+ T cells. Here we show that, unexpectedly, the PanDR helper T cell epitope PADRE, a generic T helper cell antigen presented by a large fraction of HLA-DR alleles, when delivered in particle-bound form induced maturation of human DCs. The DCs that received the particle-bound PADRE displayed all features of fully mature DCs, such as high expression of the co-stimulatory molecules CD80, CD86, CD83, the MHC-II molecule HLA-DR, secretion of high levels of the biologically active IL-12 (IL-12p70) and induction of vigorous proliferation of naïve CD4+ T cells. Furthermore, the maturation of DCs induced by particle-bound PADRE was shown to involve sphingosine kinase, calcium signaling from internal sources and downstream signaling through the MAP kinase and the p72syk pathways, and finally activation of the transcription factor NF-κB. Based on our findings, we propose that particle-bound PADRE may be used as a DC activator in DC-based vaccines. © 2013 Baleeiro et al. Source


Patent
Surflay Nanotec Gmbh and Severus Patent Ag | Date: 2012-02-17

The invention relates to a smokeless cigarette, cigar, or pipe having at least one depot (


Kang J.,Surflay Nanotec GmbH | Loew M.,Humboldt University of Berlin | Arbuzova A.,Humboldt University of Berlin | Andreou L.,Qiagen | Dahne L.,Surflay Nanotec GmbH
Advanced Materials | Year: 2010

An advanced system based on layer-bylayer (LbL) technology and fluorescence resonance energy transfer (FRET) for the detection of small amounts of DNA has been developed. Several advantages over conventional particle systems due to nanoroughness, flexibility and specific surface properties of LbL films were determined, making LbL-oligonucleotide particles a first choice for homogeneous diagnostic assays. (Figure Presented) © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Baleeiro R.B.,Charite - Medical University of Berlin | Baleeiro R.B.,University of Sao Paulo | Wiesmuller K.-H.,EMC Microcollections GmbH | Reiter Y.,Technion - Israel Institute of Technology | And 6 more authors.
Journal of Investigative Dermatology | Year: 2013

Needle-free vaccination, for reasons of safety, economy, and convenience, is a central goal in vaccine development, but it also needs to meet the immunological requirements for efficient induction of prophylactic and therapeutic immune responses. Combining the principles of noninvasive delivery to dendritic cells (DCs) through skin and the immunological principles of cell-mediated immunity, we developed microparticle-based topical vaccines. We show here that the microparticles are efficient carriers for coordinated delivery of the essential vaccine constituents to DCs for cross-presentation of the antigens and stimulation of T-cell responses. When applied to the skin, the microparticles penetrate into hair follicles and target the resident DCs, the immunologically most potent cells and site for induction of efficient immune responses. The microparticle vaccine principle can be applied to different antigen formats such as peptides and proteins, or nucleic acids coding for the antigens. © 2013 The Society for Investigative Dermatology. Source


Dahne L.,Surflay Nanotec GmbH | Schneider J.,Surflay Nanotec GmbH | Lewe D.,MT.Derm GmbH | Petersen H.,MT.Derm GmbH
Current Problems in Dermatology (Switzerland) | Year: 2015

We have evaluated the feasibility of layer-by-layer encapsulation technology for the improvement of dye pigments used for tattoos or permanent make-up. The formation of core-shell structures is possible by coating pigments with thin films of several different polyelectrolytes using this technology. The physicochemical surface properties, such as charge density and chemical functionality, can be reproducibly varied in a wide range. Tailoring the surface properties independently from the pigment core allows one to control the rheological behaviour of pigment suspensions, to prevent aggregation between different pigments, to reduce the cytotoxicity, and to influence the response of phagocytes in order to have similar or the same uptake and bioclearance for all pigments. These properties determine the durability and colour tone stability of tattoos and permanent make-up. © 2015 S. Karger AG, Basel. Source

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