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Soni H.,Suresh Gyan Vihar University
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

Nature has been a source of medicinal agents for thousands of year and an impressive number of modern drug have been isolated from natural sources, many based on their use in traditional medicine. Plants from the genus Coleus have been used in traditional medicine by many cultures. Flavonoids, glycosides, phenolic compounds and volatile constituents have been reported as the major phytoconstituents of the Coleus species. This review describes the morphology, traditional and folklore uses, phytoconstituents and pharmacological reports of the prominent species of the genus Coleus. Various virgin areas of research on the species of this genus have been highlighted with a view to explore, isolate and identify the medicinally important phyto-constituents which could be utilized to alleviate various diseases affecting the mankind. Source


Rastogi H.,Suresh Gyan Vihar University | Jana S.,Laila Nutraceuticals Research and Development Center
European Journal of Drug Metabolism and Pharmacokinetics | Year: 2016

Phenolic compounds are common ingredients in many dietary supplements and functional foods. However, data concerning physicochemical properties and permeability of polyphenols on the intestinal epithelial cells are scarce. The aims of this study were to determine the experimental partition coefficient (Log P), and parallel artificial membrane permeability assay (PAMPA), to characterize the bi-directional transport of six phenolic compounds viz. caffeic acid, chrysin, gallic acid, quercetin, resveratrol and rutin in Caco-2 cells. The experimental Log P values of six polyphenols were correlated (R 2 = 0.92) well with the calculated Log P values. The apparent permeability (P app) range of all polyphenols in PAMPA for the apical (AP) to basolateral (BL) was 1.18 ± 0.05 × 10-6 to 5.90 ± 0.16 × 10-6 cm/s. The apparent Caco-2 permeability (P app) range for the AP-BL was 0.96 ± 0.03 × 10-6 to 3.80 ± 0.45 × 10-6 cm/s. The efflux ratio of P app (BL → AP) to P app (AP → BL) for all phenolics was <2, suggesting greater permeability in the absorptive direction. Six compounds exhibited strong correlations between Log P and PAMPA/Caco-2 cell monolayer permeation data. Dietary six polyphenols were poorly absorbed through PAMPA and Caco-2 cells, and their transepithelial transports were mainly by passive diffusion. © 2014 Springer International Publishing Switzerland. Source


Gyawali S.,Suresh Gyan Vihar University | Kc B.,Rajasthan Pharmacy College | Shankar P.R.,Novel Academy | Shankar P.R.,KIST Medical College
BMC International Health and Human Rights | Year: 2013

Background: Unsafe injection practices and injection overuse are widespread in developing countries harming the patient and inviting risks to the health care workers. In Nepal, there is a dearth of documented information about injection practices so the present study was carried out: a) to determine whether the selected government health facilities satisfy the conditions for safe injections in terms of staff training, availability of sterile injectable equipment and their proper disposal after use and b) to assess knowledge and attitudes of healthcare workers in these health care facilities with regard to injection safety. Methodology. A descriptive cross-sectional mixed type (qualitative and quantitative) survey was carried out from 18§ssup§ th§esup§ May to 16§ssup§th§esup§ June 2012. In-depth interviews with the in-charges were conducted using a semi-structured questionnaire. Observation of the health facilities using a structured observation tool was done. The data were analysed manually by summarizing, tabulating and presenting in various formats. Results: The in-charges (eight males, two females) who participated in the study ranged in age from 30 to 50 years with a mean age of 37.8 years. Severe infection followed by pain was the most important cause for injection use with injection Gentamicin being most commonly prescribed. New single use (disposable) injections and auto-disable syringes were used to inject curative drugs and vaccines respectively. Sufficient safety boxes were also supplied to dispose the used syringe. All health care workers had received full course of Hepatitis B vaccine and were knowledgeable about at least one pathogen transmitted through unsafe injection practices. Injection safety management policy and waste disposal guideline was not available for viewing in any of the facilities. The office staff who disposed the bio-medical wastes did so without taking any safety measures. Moreover, none of these staff had received any formal training in waste management. Conclusions: Certain safe injection practices were noticed in the studied health care facilities but there remain a number of grey areas where unsafe practices still persists placing patient and health workers at risk of associated hazards. Training concentrating on injection safety, guidelines to dispose biomedical waste and monitoring of the activity is needed. © 2013 Gyawali et al; licensee BioMed Central Ltd. Source


Ribonucleotide reductase subunit R2 regulates catalytic action of the enzyme to provide DNA synthesis material via reduction. It has been continuously investigated as anticancer drug target for design and discovery of its inhibitors. Present studies aim to design novel heterocyclic/aryl substituted and adamantyl added thiosemicarbazones out fitted with improved cell permeability and effective RNR inhibition. Design strategy renders significant use of virtual screening and molecular docking studies to converge search of selective molecules for synthesis and further experimental studies. Selected candidates were synthesized and evaluated in vitro for their RNR inhibitory activity (IC50, uM) on MCF-7 cells, breast cancer cell lines. Molecular docking results (docking scores) and experimental results (IC50, uM) were found to be correlated and in agreement. Structure-based and ligandbased studies of results substantiate regulative role of water molecules at catalytic site (H2O: 2057) as well as at RNR inhibitor binding site (H2O: 2023, 2047, 2060 and 2070). Admantyl group has testified constant spatial position in docked poses and involved in steric interactions with Cys271, Asp272, Phe237, Gly234 and Val238. Heterocyclic/aryl substitutions equally offered H-bonds with water molecules (H2O: 2028, 2054, 2061 and 2073) along with amino acids Ser264, Asp272, Tyr324 and Asn346. Present efforts to design new inhibitors incur new characteristics in RNR Inhibition. © 2015 Bentham Science Publishers. Source


Mittal S.,Suresh Gyan Vihar University
International Journal of Pharma and Bio Sciences | Year: 2013

Medicinal plant contains matter that can be used for therapeutic reason or which are precursors for the production of useful drugs. Very few and periodic works are there in the literature regardingthe phytochemistry and TLC of Viola odorata L. TLC profiling of plant extract gives an idea about thepresence of various phytochemicals. Different Rf (Retention factor) value of various phytochemicals provide valuable clue regarding their polarity and selection of solvents for separation of phytochemicals. HPLC profile of petroleum ether extract of Viola odorata have characteristics peaks at retentiontime 2.885, 3.370, 3.487, 3.900, 4.067, 4.191, 5.207, 6.724 (Stigmasterol), 17.698 (Lupeol), 18.174 (β-Sitosterol), 23.118. These peaks showed that there are different compounds and characteristic fingerprints for each drug to judge in an herbal formulation. Source

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