Zhang Z.,Ghent University |
Schepens B.,Ghent University |
Schepens B.,Inflammation Research Center |
Nuhn L.,Ghent University |
And 14 more authors.
Chemical Communications | Year: 2016
We report on a straightforward strategy to fabricate bioactive glycosylated gold nanoparticles via a combination of RAFT polymerization, carbohydrate ligation through reductive amination and thiol-gold self-assembly. This approach is used for the design of gold nanoparticles decorated with the complex sialylated glycan Neu5Ac-α-2-6-Gal, and we demonstrate multivalent and specific recognition between the nanoparticles, lectins and hemagglutinin on the surface of the influenza virus. © 2016 The Royal Society of Chemistry.
Vanparijs N.,Ghent University |
Maji S.,Supramolecular Chemistry Group |
Louage B.,Ghent University |
Voorhaar L.,Supramolecular Chemistry Group |
And 6 more authors.
Polymer Chemistry | Year: 2015
Efficient polymer-protein conjugation is a crucial step in the design of many therapeutic protein formulations including nanoscopic vaccine formulations, antibody-drug conjugates and to enhance the in vivo behaviour of proteins. Here we aimed at preparing well-defined polymers for conjugation to proteins by reversible addition-fragmentation chain transfer (RAFT) polymerization of both acrylates and methacrylamides with protein-reactive chain transfer agents (CTAs). These RAFT agents contain either a N-hydroxysuccinimide (NHS) or pentafluorophenyl (PFP) ester moiety that can be conjugated to lysine residues, and alternatively a maleimide (MAL) or pyridyl disulfide (PDS) moiety that can be conjugated to cysteine residues. Efficiency of the bioconjugation of these polymers to bovine and avian serum albumin was investigated as a function of stoichiometry, polymer molecular weight and the presence of reducing agents. A large molar excess of polymer was required to obtain an acceptable degree of protein conjugation. However, protein modification with N-succinimidyl-S-acetylthiopropionate (SATP) to introduce sulfhydryl groups onto primary amines, significantly increased conjugation efficiency with MAL- and PDS-containing polymers. This journal is © The Royal Society of Chemistry.
Vancoillie G.,Supramolecular Chemistry Group |
Hoogenboom R.,Supramolecular Chemistry Group
Polymer Chemistry | Year: 2016
Boronic acid decorated copolymers have gathered significant interest in recent years. These (co)polymers are well-known for their saccharide responsive properties commonly applied in polymeric glucose sensors, cell capture and enzymatic inhibition. Despite this wide variety of applications, boronic acid containing monomers and resulting (co)polymers are also known for their notoriously difficult synthesis and purification. This review provides a condensed overview of the different synthetic pathways that have been reported for both monomer and (co)polymer synthesis. The first part of this review will focus on the synthesis of different boronic acid containing monomers bearing various polymerizable groups including (meth)acrylates, (meth)acrylamides and styrenics. These monomers show a wide variety in synthetic complexity but also in the Lewis acidity of the boronic acid moiety, which is of vital importance for success of the desired application. The second part will then discuss the main approaches for the (co)polymerization of these monomers divided into three groups, namely polymerization of unprotected boronic acid monomers, polymerization of protected boronate ester monomers and finally boronic acid incorporation through post-polymerization modification reactions. This review will complement the already numerous application-focused papers and provide a comprehensive overview of the currently used synthetic methodologies as guideline for boronic acid-containing (co)polymer research. © 2016 The Royal Society of Chemistry.
Rovers S.A.,TU Eindhoven |
Hoogenboom R.,Dolphys Medical |
Hoogenboom R.,Supramolecular Chemistry Group |
Kemmere M.F.,TU Eindhoven |
Keurentjes J.T.F.,TU Eindhoven
Soft Matter | Year: 2012
Drug release from a polymeric matrix has been externally triggered using an alternating magnetic field in order to develop an on-demand drug delivery implant. Superparamagnetic iron oxide nanoparticles have been distributed in a poly(methyl methacrylate) core, coated with a thermoresponsive layer of poly(butyl methacrylate-stat-methyl methacrylate) containing ibuprofen as a model drug. The release rate of ibuprofen reversibly increased, up to 25-fold, upon exposure to the magnetic field and was found to increase with higher iron oxide loading. Finally, magnetically triggered on-demand drug release was demonstrated under physiologically relevant conditions, namely 37 °C in PBS buffer with high ibuprofen content in the implant and only 15 minutes triggering time. © 2012 The Royal Society of Chemistry.
PubMed | Supramolecular Chemistry Group, Ghent University and University of Mons
Type: Journal Article | Journal: Chemical communications (Cambridge, England) | Year: 2016
We report on a straightforward strategy to fabricate bioactive glycosylated gold nanoparticles via a combination of RAFT polymerization, carbohydrate ligation through reductive amination and thiol-gold self-assembly. This approach is used for the design of gold nanoparticles decorated with the complex sialylated glycan Neu5Ac--2-6-Gal, and we demonstrate multivalent and specific recognition between the nanoparticles, lectins and hemagglutinin on the surface of the influenza virus.
News Article | December 15, 2016
Rigaku Oxford Diffraction is pleased to announce its attendance at the annual meeting of the Royal Society of Chemistry Macrocyclic and Supramolecular Chemistry Group (MASC 2016), to be held at the John McIntyre Conference Centre, Pollock Halls, The University of Edinburgh on December 15 - 16, 2016. This two day conference is regarded as the preeminent annual meeting of the UK macrocycle and supramolecular community. Rigaku is hosting an exhibit featuring the company’s systems for small molecule crystallography, including the Rigaku XtaLAB Synergy-S and XtaLAB Synergy-R diffractometers. In addition, Rigaku will present a talk entitled “Advances in Crystallography,” highlighting the benefits of the company’s instrumentation for the study of macrocyclic and supramolecular materials. ROD was formed as the global single crystal business unit of Rigaku Corporation after the acquisition of the former Oxford Diffraction organization from Agilent Technologies in 2015. ROD is a leader in the field of single crystal analysis, both in the field of chemical crystallography as well as well as macromolecular crystallography. Formed in 1951, Rigaku Corporation is a leading analytical instrumentation company based out of Tokyo, Japan