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Horgen / Allmend, Switzerland

Robic A.,French National Institute for Agricultural Research | Larzul C.,French National Institute for Agricultural Research | Grindflek E.,NORSVIN | Chevillon P.,Institute du Porc | And 6 more authors.
Journal of Animal Breeding and Genetics | Year: 2012

A quantitative trait loci (QTL) for accumulation of androstenone in fat has been identified in an Large White×Meishan cross in a region of SSC7-containing TEAD3. In humans, TEAD3 is a transcription activator, known to be able to regulate the transcription of HSD3B. This enzyme is involved in the degradation of androstenone in the liver. In this study, porcine transcripts of TEAD3 were characterized and compared with mammalian transcripts. The complete structure of porcine TEAD3 gene was characterized including two 5′ non-coding exons and one exon 5 not used in porcine transcripts. Variations were screened in sequences related to TEAD3: in exons, in flanking sequences of exons and in the promoter region. A SNP characterized at 726bp at 5′ of the first exon was tested on several pig populations without coherent and convincing results concerning its association with androstenone levels. We showed that in the liver of adult boars, the transcripts levels of TEAD3 and HSD3B were correlated. As in humans, it is possible that HSD3B is a target gene of TEAD3 in porcine liver. Nevertheless, no expression variation was observed for TEAD3 or HSD3B in liver between animals with different genotypes at the SNP. We concluded that this SNP was not the causal mutation of this QTL. © 2011 Blackwell Verlag GmbH.

Masclaux F.G.,University of Geneva | Sakwinska O.,University of Lausanne | Sakwinska O.,Nestle | Charriere N.,University of Geneva | And 2 more authors.
Annals of Occupational Hygiene | Year: 2013

Pigs are very often colonized by Staphylococcus aureus and transmission of such pig-associated S. aureus to humans can cause serious medical, hygiene, and economic problems. The transmission route of zoonotic pathogens colonizing farm animals to humans is not well established and bioaerosols could play an important role. The aim of this study was to assess the potential occupational risk of working with S. aureus-colonized pigs in Switzerland. We estimated the airborne contamination by S. aureus in 37 pig farms (20 nursery and 17 fattening units; 25 in summer, 12 in winter). Quantification of total airborne bacterial DNA, airborne Staphylococcus sp. DNA, fungi, and airborne endotoxins was also performed. In this experiment, the presence of cultivable airborne methicillin-resistant S. aureus (MRSA) CC398 in a pig farm in Switzerland was reported for the first time. Airborne methicillin-sensitive S. aureus (MSSA) was found in ~30% of farms. The average airborne concentration of DNA copy number of total bacteria and Staphylococcus sp. measured by quantitative polymerase chain reaction was very high, respectively reaching values of 75 (± 28) × 107 and 35 (± 9.8) × 105 copy numbers m-3 in summer and 96 (± 19) × 108 and 40 (± 12) × 106 copy numbers m-3 in winter. Total mean airborne concentrations of endotoxins (1298 units of endotoxin m -3) and fungi (5707 colony-forming units m-3) exceeded the Swiss recommended values and were higher in winter than in summer. In conclusion, Swiss pig farmers will have to tackle a new emerging occupational risk, which could also have a strong impact on public health. The need to inform pig farmers about biological occupational risks is therefore crucial. © 2013 The Author.

The aim of this study was to describe the practical implementation of the painless castration under injection anaesthesia in Switzerland. 30 swine farms were visited and 60 farmers answered a questionnaire. 34% of the piglets showed movements during castration under injection anaesthesia and 17% had excitations during recovery from anaesthesia. After 48 minutes half of the piglets were in sternal position and after 112 minutes half of them showed coordinated movements. The body temperature decreased by 3.1 °C until 60 minutes after castration, especially small piglets reached critical temperature levels. 38% of the piglets showed strong bleeding after castration. The healing of the wound was good according to 82% of the farmers. 83% of the farmers reported piglet losses, especially at the beginning of the anaesthesia period. The anaesthesia may be improved by using butorphanol in addition to the combination of ketamine and azaperone. The recovery of the piglets should be in a warm place without any risk of injury by obstacles or the sow. Increased bleeding can be controlled by using an emasculator. © 2013 Verlag Hans Huber, Hogrefe AG, Bern.

Enz A.,Abteilung Schweinemedizin | Schupbach-Regula G.,University of Bern | Bettschart R.,Abteilung fur Anasthesiologie | Fuschini E.,SUISAG | And 2 more authors.
Schweizer Archiv fur Tierheilkunde | Year: 2013

The objectives of this study were a description of the practical implementation of the painless castration under inhalational anaesthesia with an objective assessment of animal welfare, workplace safety and time exposure. 600 questionnaires were sent to farmers all over Switzerland and 100 farms were visited during castration under inhalation anaesthesia. 44% of the visited farmers administered analgetics during anaesthesia or less than 10 minutes before castration. 14% of the piglets were insufficiently anaesthetised (moving or vocalising) and 18% showed stronger bleeding tendency after castration. The mortality rate was less than 0.1%. 22% of the swine farmers reported headache or dizziness during or after castration work. The Isoflurane level on 2 farms was above the Swiss safety limits. The time needed for castration was with 4.3 minutes clearly above the time necessary without anaesthesia. The additional financial costs and time are at the moment not adequately compensated to the farmers. © 2013 Verlag Hans Huber, Hogrefe AG, Bern.

Becker D.,University of Bern | Wimmers K.,Leibniz Institute for Farm Animal Biology | Luther H.,SUISAG | Hofer A.,SUISAG | Leeb T.,University of Bern
PLoS ONE | Year: 2013

The improvement of meat quality and production traits has high priority in the pork industry. Many of these traits show a low to moderate heritability and are difficult and expensive to measure. Their improvement by targeted breeding programs is challenging and requires knowledge of the genetic and molecular background. For this study we genotyped 192 artificial insemination boars of a commercial line derived from the Swiss Large White breed using the PorcineSNP60 BeadChip with 62,163 evenly spaced SNPs across the pig genome. We obtained 26 estimated breeding values (EBVs) for various traits including exterior, meat quality, reproduction, and production. The subsequent genome-wide association analysis allowed us to identify four QTL with suggestive significance for three of these traits (p-values ranging from 4.99×10-6 to 2.73×10-5). Single QTL for the EBVs pH one hour post mortem (pH1) and carcass length were on pig chromosome (SSC) 14 and SSC 2, respectively. Two QTL for the EBV rear view hind legs were on SSC 10 and SSC 16. © 2013 Becker et al.

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