Sugen Life science Pvt. Ltd
Sugen Life science Pvt. Ltd
Chilakapati S.R.,Sugen Life science Pvt Ltd |
Chilakapati S.R.,Sri Venkateswara University |
Serasanambati M.,Sugen Life science Pvt Ltd |
Serasanambati M.,Sri Venkateswara University |
And 3 more authors.
International Journal of Drug Delivery | Year: 2014
Idiopathic Pulmonary Fibrosis is a chronic lung disease characterized by lung inflammation and progression to fibrosis. Anti-fibrotic efficacy of lutein was studied in mouse model of bleomycin induced pulmonary fibrosis. The mice were orally administrated with 100 or 200 mg/kg of lutein three days prior to intra-tracheal instillation of bleomycin on day 0 and continued up to day 21. Total cell counts and protein levels in branchoalveolar lavage fluid and superoxide dismutase, and hydroxyproline levels were evaluated. Treatment with lutein at 100 and 200 mg/kg prevented bleomycin induced mortality and body weight loss. Lutein administration attenuated bleomycin induced increase of total and differential cell count and myeloperoxidase, inflammatory cell infiltration in lung tissue. Similarly lutein administration restored superoxide dismutase activity lowered by bleomycin instillation. The combined results revealed the protective effect of lutein by suppressing the inflammation, oxidative stress and enhancing the antioxidant potential which may helps in pulmonary fibrosis. © 2014, Advanced Research Journals. All rights reserved.
Rani V.J.,Sri Venkateswara University |
Rani V.J.,Sugen Life science Pvt. Ltd. |
Aminedi R.,Thapar University |
Polireddy K.,University of Kansas Medical Center |
Jagadeeswarareddy K.,Sugen Life science Pvt. Ltd.
Archiv der Pharmazie | Year: 2012
The pyrimidine nucleus is an important component of nucleic acids (DNA and RNA) and vitamins (B2 and folic acid). It is evident from the literature that pyrimidine derivatives possess a wide spectrum of biological activities such as antioxidant, anticancer, antibacterial, and anti-inflammatory activities. On the basis of diverse biological activities, we attempted to synthesize a series of novel bis(2-(pyrimidin-2-yl)ethoxy)alkanes 5a-j in four steps with good yields. 2-Chloropyrimidine (1) was reacted with diethyl malonate in the presence of sodium hydride in dry dimethyl formamide to yield the intermediate diethyl 2-(pyrimidin-2-yl)malonate (2), which on further reaction with sodium chloride and dimethyl sulfoxide yielded ethyl 2-(pyrimidin-2-yl) ethanoate (3). Reduction with sodium borohydride (NaBH4) resulted in the formation of 2-(pyrimidin-2-yl)ethanol (4). This was further reacted with various dibromoalkanes to obtain the title compounds 5a-j. In this current study, we evaluated the antioxidant properties of the title compounds using four in vitro test systems: the 2,2-diphenyl-2-picrylhydrazyl radical-, superoxide radical-, and hydroxyl radical-scavenging assays, and the anti-lipid peroxidation activity test. The title compounds showed promising antioxidant activity when compared to butylated hydroxytoluene. The potency of their antioxidant activity was mainly influenced by the alkyl fragment attached to 2-(pyrimidin-2-yl)ethanol. The ethyl and butyl fragments linked to oxygen led to increased antioxidant activity of the title compounds (i.e., 5b and 5d) in all our in vitro assays. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Chilakapati S.R.,SugenLife science Pvt. Ltd |
Serasanambati M.,SugenLife science Pvt. Ltd |
Manikonda P.K.,SugenLife science Pvt. Ltd |
Chilakapati D.R.,SugenLife science Pvt. Ltd |
Watson R.R.,South West Consulting and Editing
Canadian Journal of Physiology and Pharmacology | Year: 2014
Idiopathic pulmonary fibrosis is a progressive fatal lung disease characterized by excessive collagen deposition, with no effective treatments. We investigated the efficacy of natural products with high anti-inflammatory activity, such as passion fruit peel extract (PFPE), in a mouse model of bleomycin-induced pulmonary fibrosis (PF). C57BL/6J mice were subjected to a single intratracheal instillation of bleomycin to induce PF. Daily PFPE treatment significantly reduced loss of body mass and mortality rate in mice compared with those treated with bleomycin. While bleomycin-induced PF resulted in elevated total numbers of inflammatory cells, macrophages, lymphocytes, and neutrophils in bronchoalveolar lavage fluid on both days 7 and 21, PFPE administration significantly attenuated these phenomena compared with bleomycin group. On day 7, the decreased superoxide dismutase and myeloperoxidase activities observed in the bleomycin group were significantly restored with PFPE treatment. On day 21, enhanced hydroxyproline deposition in the bleomycin group was also suppressed by PFPE administration. PFPE treatment significantly attenuated extensive inflammatory cell infiltration and accumulation of collagen in lung tissue sections of bleomycin-induced mice on days 7 and 21, respectively. Our results indicate that administration of PFPE decreased bleomycin-induced PF because of anti-inflammatory and antioxidant activities.
Manikonda P.K.,Osmania University |
Manikonda P.K.,Sugen Life science Pvt. Ltd. |
Rajendra P.,Indian Central Power Research Institute |
Rajendra P.,Central University of Kerala |
And 7 more authors.
General Physiology and Biophysics | Year: 2014
The present investigation was conducted to understand the influence of long-term exposure of rats to extremely low frequency magnetic fields (ELF-MF), focusing on oxidative stress (OS) on different regions of rat's brain. Male Wistar rats (21-day-old) were exposed to ELF-MF (50 Hz; 50 and 100 μT) for 90 days continuously; hippocampal, cerebellar and cortical regions from rats were analyzed for (i) reactive oxygen species (ROS), (ii) metabolites indicative of OS and (iii) antioxidant enzymes. In comparison to control group rats, the rats that were continuously exposed to ELF-MF caused OS and altered glutathione (GSH/GSSG) levels in dose-dependent manner in all the regions of the brain. Accumulation of ROS, lipid peroxidation end products and activity of superoxide dismutase in different regions was in the descending order of cerebellum > hippocampus > cortex. Decrement in GSH/GSSG levels and increment in glutathione peroxidase activity were in the descending order of hippocampus > cerebellum > cortex. The continuous exposure to ELF-MF caused OS in all the examined regions of brain more significantly at 100 μT than at 50 μT. Varied influences observed in different regions of the brain, as documented in this study, may contribute to altered metabolic patterns in its related regions of the central nervous system, leading to aberrant neuronal functions.
Jhansi Rani V.,Sri Venkateswara University |
Jhansi Rani V.,Sugen Life science Pvt Ltd |
Raghavendra A.,Thapar University |
Kishore P.,University of Kansas Medical Center |
And 3 more authors.
European Journal of Medicinal Chemistry | Year: 2012
Capecitabine, an oral prodrug of 5-FU was developed to improve the tumor selectivity and tolerability. To enhance the efficacy of capacitabine, a series of 5′-deoxy-5-fluorocytidine derivatives 5a-e were synthesized. In the present study, we investigated antitumor activity of 5′-deoxy-5- fluorocytidine derivatives both in vivo and in vitro methods. Title compounds were non-mutagenic to Salmonella typhimurium tester strain in Ames test. Compounds 5d and 5e are potent to inhibit the proliferation of NCI-69, PZ-HPV-7, MCF-7 and HeLa cells in MTT assay. In particular, 5d and 5e showed potent antitumor activities against L1210 leukemia cell line. Collectively, these findings suggest that 5d and 5e are more potent anti-cancer compounds than capecitabine. © 2012 Elsevier Masson SAS. All rights reserved.
Raju I.N.,Sugen Life science Pvt Ltd |
Reddy K.K.,Sri Venkateswara University |
Kumari C.K.,Sugen Life science Pvt Ltd |
Reddy E.B.,Sugen Life science Pvt Ltd |
And 4 more authors.
Journal of Evidence-Based Complementary and Alternative Medicine | Year: 2013
The clinical efficacy of purple passion fruit peel extract (a flavonoid-rich dietary supplement) in reducing cardiovascular risk factors in adult type 2 diabetic subjects was investigated in a randomized, double-blind, placebo-controlled trial. Forty-one subjects were randomly assigned to receive a daily dose of purple passion fruit (220 mg) or a matched placebo for 16 weeks. Body mass index, blood pressure, fasting and postprandial blood glucose, glycated hemoglobin, and lipid profile were determined at baseline and at monthly intervals. A significant reduction in systolic blood pressure and fasting blood glucose was observed following administration of purple passion fruit (P<.05). Purple passion fruit was well tolerated, and no adverse events were reported. These data suggest that purple passion fruit supplementation for 16 weeks in type 2 diabetics results in a significant reduction in systolic blood pressure and fasting blood glucose, indicating that purple passion fruit is safe and well tolerated by diabetics. © The Author(s) 2013.
Chilakapati S.R.,Sugen Life science Pvt. Ltd. |
Chilakapati S.R.,Sri Venkateswara University |
Serasanambati M.,Sugen Life science Pvt. Ltd. |
Serasanambati M.,Sri Venkateswara University |
And 3 more authors.
Experimental Lung Research | Year: 2015
Introduction: Idiopathic pulmonary fibrosis (IPF) is characterized by alveolitis, progressing into fibrosis. Due to the involvement of both endothelin and platelet-derived growth factor signaling in IPF, combination effects of a bosentan and imatinib were studied in mouse model of bleomycin-induced pulmonary fibrosis. Methods: Mice subjected to bleomycin instillation (0.05 U) and were administered with either bosentan (100 mg/kg) and/or imatinib (50 mg/kg). Inflammatory cell count, total protein estimation in bronchoalveolar lavage fluid, lung edema, superoxide dismutase, catalase, myeloperoxidase activities, and Hematoxylin & Eosin staining were performed on day 7. Hydroxyproline content, α-smooth muscle actin (SMA), collagens I and III gene expression analysis, immunohistochemistry, matrix metalloproteinases-9 and -2 activities, trichrome and sirius red staining were performed on day 21. Results: Combination treatment with bosentan and imatinib prevented bleomycin-induced mortality and loss of body weight more than the individual agents. On day 7, the combination therapy attenuated bleomycin-induced increase of total and differential inflammatory cell counts, total proteins, lung wet/dry weight ratio, myeloperoxidase activity, lung inflammatory cell infiltration more than individual agents alone. Bosentan but not imatinib ameliorated superoxide dismutase and catalase activities, which were lowered following bleomycin instillation. On day 21, combination therapy ameliorated bleomycin-induced increase of fibrosis score, collagen deposition, protein and gene expression of SMA, mRNA levels of collagens-I and -III, matrix metalloproteinase-9 and -2 activities more than monotherapy. Conclusion: Combination of bosentan and imatinib exerted more enhanced protection against bleomycin-induced inflammation and fibrosis than either of the agents alone. © 2015 Informa Healthcare USA, Inc.
Krishna Kumari C.,Sugen Life science Pvt Ltd. |
Shanmuga Reddy C.,Sugen Life science Pvt Ltd. |
Raja Ratna Reddy Y.,Sugen Life science Pvt Ltd. |
Narapa Reddy D.,TTD |
Damodar Reddy C.,Sugen Life science Pvt Ltd.
International Journal of Pharma and Bio Sciences | Year: 2013
Cyclodextrin glycosyl transferase (CGTase; EC 126.96.36.199) is an enzyme which converts starch into cyclodextrins (CDs). Cyclodextrins are closed-ring structures in which six or more glucose units are joined by a-1, 4 glucosidic bonds. Cyclodextrins have applications in food, pharmaceutical and cosmetic industries. This paper reports the exploration of aerobic alkaliphilic bacteria from alkaline sodalake of Lonar, India for CGTase production. Paenibacillus sp L55 isolated from Lonar lake was used for production of CGTase. Three medium components Starch, Peptone and Yeast extract and initial pH of medium were optimised by Taguchi Design of Experiments (DOE). The contribution of starch in CGTase production was the highest while pH showed the least contribution in CGTase production. The optimised medium was used for CGTase production and the yield of CGTase was increased from 3 U/ml to 5.1 U/ml.
Serasanambati M.,Sugen Life science Pvt. Ltd |
Chilakapati S.R.,Sugen Life science Pvt. Ltd |
Manikonda P.K.,Sugen Life science Pvt. Ltd |
Kanala J.R.,Sugen Life science Pvt. Ltd
Current Trends in Biotechnology and Pharmacy | Year: 2013
Breast cancer is the most commonly diagnosed disease among women. In recent years, chemotherapeutic drugs such as gemcitabine, erlotinib, etc. have been developed for the treatment of breast cancer. Breast cancers relapse due to the generation of chemoresistance that makes therapeutic drugs ineffective. Hence, agents that can reduce the chemoresistance to the therapeutic drugs are being developed for better advancement of in cancer therapy. The present study evaluates the efficiency of curcumin, in suppression of gemcitabine induced NF-kB activity and in the enhancement of antitumor effect of gemcitabine on MCF-7 and MDA MB-231breast cancer cells. 10 or 20 μM of curcumin and 10 or 100 μM of gemcitabine, alone or in combination, were used. Cell proliferation by MTT assay, apoptotic effects by Live/Dead assay, nuclear factor-kB (NF-kB) activation or suppression by EMSA were determined. The results indicated a decrease in cell proliferation of up to 61% (p ≤ 0.01) and 45% (p ≤ 0.01) at 20 μM curcumin and 100 μM of gemcitabine in MCF-7 and MDA MB-231, respectively. Whereas 20 μM curcumin potentiated the apoptotic effects of gemcitabine (100μM) predominantly in MCF-7 by 61% and in MDA MB-231 by 46% which was determined by using Live/Dead assay. However, curcumin (20 μM) significantly (p ≤ 0.05) suppressed NF-kB activation by 80% which was induced by gemcitabine (100μM) in both cell lines. The data obtained from the present investigation shows the dose dependent changes in MCF-7 and MDA MB-231. The combined results revealed the beneficial role of curcumin in potentiating the anti-tumor effects of gemcitabine through NF-kB suppression and apoptotic effects.
Vissavajjhala P.,Sugen Life science Pvt Ltd
Indian Journal of Agricultural Biochemistry | Year: 2014
The alarmingly increasing trends of global overweight, obesity among both the adults as well as the children need an immediate attention for nutritional intervention as billions of dollars are spent for the treatment of obesity and related diseases every year with clinical and pharmaceutical interventions. Apart from the inherent genetic reasons, which are very small in quantity, diet induced obesity along with sedentary life style are the major reasons for such trends leading to metabolic syndrome. Metabolic syndrome is characterized by insulin resistance, type 2 diabetes, proinflammatory status, endothelial dysfunction, dyslipidemia resulting in several cardiovascular diseases. Not only consuming the right foods, but even avoiding the wrong foods is equally important in nutrition. Hence, improper intake of foods both quantitatively and qualitatively contributes to the increasing trends of overweight and obesity among the population where nutritional intervention can help restoring normalcy with reference to the elevated clinical biomarkers associated with these complications. Several nutrients from natural foods, fruits and vegetables comprising of a wide range of phytochemicals, high fibre that help lowering blood glucose, cholesterol, hypertension, improving heart health and lowering the incidence of some cancers emphasize how food and nutrients serve as medicine for the cause of enhanced quality of life, maintaining health and wellness. Certain aspects of epigenetics with regards to nutrients that influence gene expression especially, with reference to cancer further emphasize the concept of food as medicine on one hand and on the other, call for the approach and necessity for the implementation of ‘personalized nutrition’. © 2014, Indian Society of Agricultural Biochemists. All rights reserved.