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Tirupati, India

Manikonda P.K.,Osmania University | Manikonda P.K.,Sugen Life science Pvt. Ltd | Rajendra P.,Indian Central Power Research Institute | Rajendra P.,Central University of Kerala | And 7 more authors.
General Physiology and Biophysics | Year: 2014

The present investigation was conducted to understand the influence of long-term exposure of rats to extremely low frequency magnetic fields (ELF-MF), focusing on oxidative stress (OS) on different regions of rat's brain. Male Wistar rats (21-day-old) were exposed to ELF-MF (50 Hz; 50 and 100 μT) for 90 days continuously; hippocampal, cerebellar and cortical regions from rats were analyzed for (i) reactive oxygen species (ROS), (ii) metabolites indicative of OS and (iii) antioxidant enzymes. In comparison to control group rats, the rats that were continuously exposed to ELF-MF caused OS and altered glutathione (GSH/GSSG) levels in dose-dependent manner in all the regions of the brain. Accumulation of ROS, lipid peroxidation end products and activity of superoxide dismutase in different regions was in the descending order of cerebellum > hippocampus > cortex. Decrement in GSH/GSSG levels and increment in glutathione peroxidase activity were in the descending order of hippocampus > cerebellum > cortex. The continuous exposure to ELF-MF caused OS in all the examined regions of brain more significantly at 100 μT than at 50 μT. Varied influences observed in different regions of the brain, as documented in this study, may contribute to altered metabolic patterns in its related regions of the central nervous system, leading to aberrant neuronal functions.

Vissavajjhala P.,Sugen Life science Pvt. Ltd
Indian Journal of Agricultural Biochemistry | Year: 2014

The alarmingly increasing trends of global overweight, obesity among both the adults as well as the children need an immediate attention for nutritional intervention as billions of dollars are spent for the treatment of obesity and related diseases every year with clinical and pharmaceutical interventions. Apart from the inherent genetic reasons, which are very small in quantity, diet induced obesity along with sedentary life style are the major reasons for such trends leading to metabolic syndrome. Metabolic syndrome is characterized by insulin resistance, type 2 diabetes, proinflammatory status, endothelial dysfunction, dyslipidemia resulting in several cardiovascular diseases. Not only consuming the right foods, but even avoiding the wrong foods is equally important in nutrition. Hence, improper intake of foods both quantitatively and qualitatively contributes to the increasing trends of overweight and obesity among the population where nutritional intervention can help restoring normalcy with reference to the elevated clinical biomarkers associated with these complications. Several nutrients from natural foods, fruits and vegetables comprising of a wide range of phytochemicals, high fibre that help lowering blood glucose, cholesterol, hypertension, improving heart health and lowering the incidence of some cancers emphasize how food and nutrients serve as medicine for the cause of enhanced quality of life, maintaining health and wellness. Certain aspects of epigenetics with regards to nutrients that influence gene expression especially, with reference to cancer further emphasize the concept of food as medicine on one hand and on the other, call for the approach and necessity for the implementation of ‘personalized nutrition’. © 2014, Indian Society of Agricultural Biochemists. All rights reserved.

Raju I.N.,Sugen Life science Pvt. Ltd | Reddy K.K.,Sri Venkateswara University | Kumari C.K.,Sugen Life science Pvt. Ltd | Reddy E.B.,Sugen Life science Pvt. Ltd | And 4 more authors.
Journal of Evidence-Based Complementary and Alternative Medicine | Year: 2013

The clinical efficacy of purple passion fruit peel extract (a flavonoid-rich dietary supplement) in reducing cardiovascular risk factors in adult type 2 diabetic subjects was investigated in a randomized, double-blind, placebo-controlled trial. Forty-one subjects were randomly assigned to receive a daily dose of purple passion fruit (220 mg) or a matched placebo for 16 weeks. Body mass index, blood pressure, fasting and postprandial blood glucose, glycated hemoglobin, and lipid profile were determined at baseline and at monthly intervals. A significant reduction in systolic blood pressure and fasting blood glucose was observed following administration of purple passion fruit (P<.05). Purple passion fruit was well tolerated, and no adverse events were reported. These data suggest that purple passion fruit supplementation for 16 weeks in type 2 diabetics results in a significant reduction in systolic blood pressure and fasting blood glucose, indicating that purple passion fruit is safe and well tolerated by diabetics. © The Author(s) 2013.

Krishna Kumari C.,Sugen Life science Pvt. Ltd | Shanmuga Reddy C.,Sugen Life science Pvt. Ltd | Raja Ratna Reddy Y.,Sugen Life science Pvt. Ltd | Narapa Reddy D.,TTD | Damodar Reddy C.,Sugen Life science Pvt. Ltd
International Journal of Pharma and Bio Sciences | Year: 2013

Cyclodextrin glycosyl transferase (CGTase; EC is an enzyme which converts starch into cyclodextrins (CDs). Cyclodextrins are closed-ring structures in which six or more glucose units are joined by a-1, 4 glucosidic bonds. Cyclodextrins have applications in food, pharmaceutical and cosmetic industries. This paper reports the exploration of aerobic alkaliphilic bacteria from alkaline sodalake of Lonar, India for CGTase production. Paenibacillus sp L55 isolated from Lonar lake was used for production of CGTase. Three medium components Starch, Peptone and Yeast extract and initial pH of medium were optimised by Taguchi Design of Experiments (DOE). The contribution of starch in CGTase production was the highest while pH showed the least contribution in CGTase production. The optimised medium was used for CGTase production and the yield of CGTase was increased from 3 U/ml to 5.1 U/ml.

Jhansi Rani V.,Sri Venkateswara University | Jhansi Rani V.,Sugen Life science Pvt. Ltd | Raghavendra A.,Thapar University | Kishore P.,University of Kansas Medical Center | And 3 more authors.
European Journal of Medicinal Chemistry | Year: 2012

Capecitabine, an oral prodrug of 5-FU was developed to improve the tumor selectivity and tolerability. To enhance the efficacy of capacitabine, a series of 5′-deoxy-5-fluorocytidine derivatives 5a-e were synthesized. In the present study, we investigated antitumor activity of 5′-deoxy-5- fluorocytidine derivatives both in vivo and in vitro methods. Title compounds were non-mutagenic to Salmonella typhimurium tester strain in Ames test. Compounds 5d and 5e are potent to inhibit the proliferation of NCI-69, PZ-HPV-7, MCF-7 and HeLa cells in MTT assay. In particular, 5d and 5e showed potent antitumor activities against L1210 leukemia cell line. Collectively, these findings suggest that 5d and 5e are more potent anti-cancer compounds than capecitabine. © 2012 Elsevier Masson SAS. All rights reserved.

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