Rastogi J.,Sugarcane Research Institute UPCSR |
Tiwari N.N.,Sugarcane Research Institute UPCSR |
Gupta S.,Sugarcane Research Institute UPCSR |
Bubber P.,Sugarcane Research Institute UPCSR |
Singh R.K.,Sugarcane Research Institute UPCSR
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2014
One of the major obstacles in sugarcane transformed tissue is the persistence of Agrobacterium tumefaciens after co-cultivation. Many antibiotics are used to eliminate the bacteria. Antibiotics interrupt the synthesis of bacterial cell wall and also suppressing the growth of co-cultivating tissues at high concentration. An assay was performed to fix the minimal eco-friendly lethal dose of the eliminating aent that was precisely efficient against A. tumefaciens. The disk diffusion assay and co-cultivation of leaf disc explants were performed by using the A. tumefaciens strain LBA 4404 P-35 GUS Intron having the plasmid pCAMBIAI3O1, which carries the selectable marker gene (npt II) for kanamycin. It was also correlated whether these antibiotics at high concentration inhibit growth of sugarcane callus and shoot formation. In this study five antibiotics i.e. Cefotaxime, Erythromydn, Chloramphenicol, Amoxicillin and Ciprofloxacin were tested with seven different concentrations @50,100,150,200,300,400 & 500 mg/l along with control. The antibiotics which showed maximum zones of inhibition were assayed to determine their effects on shoot growth and elimination of A. tumefaciens. Maximum inhibition was observed with Chioramphenicol @ 50 mg/I against A. tumefaciens LBA 4404 P-35 GUS Intron without any adverse effect on regenerated shoots through young leaf disc. The Cefotaxime @ 200 mg/I was found effective to eliminate A. tumefaciens and promote growth of regenerated sugarcane plantlet. This is the first report to eliminate A. tumefaciens LBA 4404 P-35 GUS Intron eco-friendly, an efficient and cost-effective transformation system in sugarcane. © Global Science Publications.
Singh R.K.,Sugarcane Research Institute UPCSR |
Singh R.B.,Sugarcane Research Institute UPCSR |
Singh S.P.,Sugarcane Research Institute UPCSR |
Mishra N.,Sugarcane Research Institute UPCSR |
And 3 more authors.
Sugar Tech | Year: 2013
Genetic diversity within the Saccharum spontaneum germplasm and commercial cultivars from sub tropical part of India has not been extensively examined with molecular markers as it was quite difficult to recognize through morphological and physiological characters. The similarity coefficient ranging from 0. 347 (CoS96268 and Calcutta) to 0. 865 (CoS 95255 and CoS 767) revealed that there was sympatric relationship between variety × variety and allopatric (0. 347) relationship between variety × species. This has also confirmed by the higher variation observed for quality traits in S. spontaneum (71. 4 %) than in commercial cultivars (23. 11 %). These diverse genotypes had not been extensively utilized in the development of other sugarcane cultivars; they can be used as progenitors for the creation of cultivars with a wider genetic base. © 2013 Society for Sugar Research & Promotion.