Srivong T.,Khon Kaen University |
Zhu Y.J.,Hawaii Agriculture Research Center |
Pongdontri P.,Khon Kaen University |
Pliansinchai U.,Mitrphol Sugarcane Research Center |
And 4 more authors.
Chiang Mai Journal of Science | Year: 2015
The response of three sugarcane genotypes with differential sucrose accumulation to MS medium + 2, 4-dichlorophenoxyacetic acid (2, 4-D) and/or kinetin (kn) was investigated. On MS + 2, 4-D (2 mg/l) + kn (0.5 mg/l), Louisiana Purple (LAP) had the highest callus induction (95.80 %), followed by H65-7052 (82.68 %) and Molokai (MOL) (62.58 %). Rapid callus induction was optimized by using different concentrations of 2, 4-D (1, 2, 3, 4 and 5 mg/l) or 2, 4-D (2 mg/l) + kn (0.5 mg/l). The results revealed that increased callus weight was significantly different among genotypes and treatments. The value averaged over all the treatments was highest in H65-7052 (1.60 g), followed by LAP (0.93 g) and MOL (0.74 g). MS + 2, 4-D (1-2 mg/l) or MS + 2, 4-D (2 mg/l) + kn (0.5 mg/l) could cause rapid callus growth in all the three sugarcane genotypes. For plant regeneration, all cultured calli on different media exhibited good response to MS. The number of organogenic calli averaged over all the treatments was highest in MOL (61.6), followed by LAP (54.6) and H65-7052 (11.3). The most suitable culture medium for MOL and LAP was MS + 2, 4-D (1 mg/l), while MS + 2,4-D (1 mg/l) or MS + 2,4-D (2 mg/l) + kn (0.5 mg/l) was most appropriate for H65-7052. Moreover, sucrose accumulation in relation to the expression of soluble acid invertase (SAI) gene was measured in calli and plantlets. The highest callus sucrose concentration was found in LAP (141.28 μmol/g FW), followed by H65-7052 (133.79 μmol/g FW) and MOL (41.05 μmol/g FW). The plantlet sucrose concentration showed a similar pattern. The SAI expression in both the callus and plantlet of H65-7052 and LAP was lower than that of MOL. In both systems, sucrose accumulation was negatively correlated with the increase in SAI expression. It may be concluded that callus and plantlet can be used as the model system for investigating sucrose accumulation in sugarcane. © 2015, Chiang Mai University. All rights reserved.
Chimnarong V.,MitrPhol Sugarcane Research Center |
Rethinam S.,MitrPhol Sugarcane Research Center |
Seechan M.,MitrPhol Sugarcane Research Center |
Pliansinchai U.,MitrPhol Sugarcane Research Center
33rd Asian Conference on Remote Sensing 2012, ACRS 2012 | Year: 2012
Thailand ranks the second exporter of sugar after Brazil. Crop monitoring and early yield assessment of cane production are important for agriculture planning and policy making at regional and national scales of cane production. Remote sensing offers an efficient and reliable means of collecting the information required in order to map crop type acreage and condition. The study area comprised of four provinces viz., Khon Kean, Chaiyaphum, Nong Bue Lum Phu and Mahasarakham in Northeastern of Thailand. Landsat5 TM digital data (Dec, 2011) was evaluated for the potential utility of remote sensing derived normalized difference vegetation index (NDVI) for sugarcane production estimation. Sum of NDVI of individual sampling fields were correlated with the actual production (ton/ha). NDVI which describes the healthiness of crop is one of the factors of yield variability. The result showed correlation 0.75 for sum NDVI image and sugarcane production. The other factors which influence variations are color leaf and age of cane. Further study progress to integrate with other variables to improve the accuracy.