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Parameswari B.,Sugarcane Breeding Institute Regional Center | Bagyalakshmi K.,Sugarcane Breeding Institute | Viswanathan R.,Sugarcane Breeding Institute | Chinnaraja C.,Sugarcane Breeding Institute
Virus Genes | Year: 2013

Sugarcane streak mosaic virus (SCSMV), a member of the genus Poacevirus, family Potyviridae, is an important viral pathogen affecting sugarcane cultivation in India. The complete nucleotide sequence of a SCSMV isolate from India (SCSMV-IND) was determined. The linear, assembled, single-stranded positive-sense RNA genome of SCSMV-IND was 9,786 nucleotides in length (excluding the poly (A) tail) and encoded a polyprotein of 3,131 amino acid residues. The genome of SCSMV-IND shared high degree of sequence identity with SCSMV-PAK (93 % at nucleotide and 97 % at amino acid), and shared only 81 % nucleotide and 94 % amino acid identities with all the four SCSMV isolates (SCSMV-JP1, -JP2, -ID, and -THA). Phylogenetic tree analysis of the complete genome sequences of SCSMV isolates revealed that they can be clustered into two groups. SCSMV-IND and -AP isolates showed 18 % (nucleotide) divergence within the highly conserved 3′ partial genome, suggesting a high level of genetic diversity among the Indian SCSMV isolates. © 2012 Springer Science+Business Media New York.


Goel V.,Sugarcane Breeding Institute Regional Center | Duhan B.S.,CCS Haryana Agricultural University
Medicinal Plants | Year: 2014

The vegetative parameters of ashwagandha (viz. plant height, number of primary branches, plant spread and dry weight of shoot) were enhanced significantly with the application of 12.5 mg P 2 O 5 kg -1 soil, whereas; dry weight of roots was enhanced up to the application level of 25 mg P 2 O 5 kg -1 soil. FYM at the rate of 12.5 t ha -1 in combination with 12.5 mg P 2 O 5 kg -1 soil significantly improved all the vegetative parameters, whereas, FYM at the rate of 12.5 t ha -1 in combination with 25 mg P 2 O 5 kg -1 soil significantly enhanced the dry weight of the roots. The alkaloids yield (mg pot -1) in ashwagandha roots increased significantly with the application of 25 mg P 2 O 5 kg -1 soil. The application of FYM at the rate of 12.5 t ha -1 improved the alkaloids yield (mg pot -1) but the significantly highest yield of total alkaloids (mg pot -1) was found in treatment combination of 12.5 t FYM ha -1 + inorganic-P at the rate of 25 mg P 2 O 5 kg -1 soil as compared with other eleven treatments. Nutrients (NPK) uptake by ashwagandha shoot increased significantly with the application level of 12.5 mg P 2 O 5 kg -1 soil over control whereas in case of ashwagandha roots, the increase in nutrients uptake at the level of 25 mg P 2 O 5 kg -1 soil over control. Application of FYM at the rate of 12.5 t ha -1 + 12.5 mg P 2 O 5 kg -1 soil was the best treatment combination for ashwagandha roots in terms of nutrients uptake. Application of fertilizer-P significantly improved the status of organic carbon, Available-P in post harvest soil but decreased the Available-N, Available-K and DTPA extractable micronutrients (Fe, Cu, Zn and Mn) up to the level of 25 mg P 2 O 5 kg -1 soil. FYM at the rate of 12.5 t ha -1 helped in maintaining the soil fertility status after harvest of the crop alone or in combination with fertilizer-P. © 2014, IndianJournals.com. All rights reserved.


Goel V.,Sugarcane Breeding Institute Regional Center | Duhan B.S.,Sugarcane Breeding Institute Regional Center | Duhan B.S.,CCS Haryana Agricultural University | Devraj,Sugarcane Breeding Institute Regional Center | Madan V.K.,Sugarcane Breeding Institute Regional Center
Annals of Biology | Year: 2013

A pot culture experiment was conducted to study the effect of FYM and nitrogen on plant growth parameters and medicinal quality of kalmegh in Typic Torripsamment of Hisar (Haryana). The growth parameters of kalmegh (viz., plant height, number of primary branches, plant spread, fresh and dry weight of shoot) wer1 enhanced significantly up to the nitrogen level of 25 mg N/kg soil in kalmegh. Though these parameters were also enhanced up to the nitrogen level of 37.5 mgN/kg soil but statistically results were on a par with that of 25 mg N/kg soil. Further application of FYM @ 12.5 t/ha significantly increased the yield parameters of kalmegh. Application of nitrogen @ 25 mg N/kg soil in combination with 12.5 t/ha FYM significantly increased these growth parameters as compared to other levels. Andrographolide content as well as andrographolide yield of kalmegh significantly increased with the application of 25 mg N/kg soil. Application of FYM @ 12.5 t/ha alone significantly increased the andrographolide content and yield of kalmegh. Application of 12.5 t FYM/ha in combination with fertilizer-N @ 25 mg N/kg soil significantly increased the andrographolide content and yield in kalmegh over the other treatments. However, application of FYM in absence of nitrogen significantly increased all the growth parameters, and content and yield of andrographolide up to the level of FYM 25 t/ha.


PubMed | Sugarcane Breeding Institute Regional Center and ICAR Sugarcane Breeding Institute
Type: | Journal: Journal of biotechnology | Year: 2016

Saccharum spontaneum L., a wild relative of sugarcane, is known for its adaptability to environmental stresses, particularly cold stress. In the present study, an attempt was made for transcriptome profiling of the low temperature (10C) tolerant S. spontaneum clone IND 00-1037 collected from high altitude regions of Arunachal Pradesh, North Eastern India. The Illumina Nextseq500 platform yielded a total of 47.63 and 48.18 million reads corresponding to 4.7 and 4.8 gigabase pairs (Gb) of processed reads for control and cold stressed (10C for 24h) samples, respectively. These reads were de novo assembled into 214,611 unigenes with an average length of 801bp. Further, all unigenes were aligned to GO, KEGG and COG databases in order to identify novel genes and pathways responsive upon low temperature conditions. The differential gene expression analysis revealed that about 2583 genes were upregulated and 3302 genes were down regulated during the stress. This is perhaps the comprehensive transcriptome data of a low temperature tolerant clone of S. spontaneum. This study would aid in identifying novel genes and also in future genomic studies pertaining to sugarcane and its wild relatives.


PubMed | Sugarcane Breeding Institute Regional Center
Type: Journal Article | Journal: Virus genes | Year: 2013

Sugarcane streak mosaic virus (SCSMV), a member of the genus Poacevirus, family Potyviridae, is an important viral pathogen affecting sugarcane cultivation in India. The complete nucleotide sequence of a SCSMV isolate from India (SCSMV-IND) was determined. The linear, assembled, single-stranded positive-sense RNA genome of SCSMV-IND was 9,786 nucleotides in length (excluding the poly (A) tail) and encoded a polyprotein of 3,131 amino acid residues. The genome of SCSMV-IND shared high degree of sequence identity with SCSMV-PAK (93% at nucleotide and 97% at amino acid), and shared only 81% nucleotide and 94% amino acid identities with all the four SCSMV isolates (SCSMV-JP1, -JP2, -ID, and -THA). Phylogenetic tree analysis of the complete genome sequences of SCSMV isolates revealed that they can be clustered into two groups. SCSMV-IND and -AP isolates showed 18% (nucleotide) divergence within the highly conserved 3 partial genome, suggesting a high level of genetic diversity among the Indian SCSMV isolates.

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