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Khayamim S.,Sugar Beet Seed Research Institute | Tavakkol Afshari R.,University of Tehran | Sadeghian S.Y.,Seed and Plant Certification and Registration Institute | Poustini K.,University of Tehran | And 2 more authors.
Journal of Agricultural Science and Technology | Year: 2014

It is well known that sugar beet is sensitive to salinity stress at the germination stage. Three separate experiments were conducted to study the effects of salinity on seed germination, plant establishment, and yield of sugar beet genotypes for screening purposes. These included: (a) A laboratory study using four water salinity levels (with EC values < 0.1 as the control, 16, 20, and 24 dS m-1) with 20 sugar beet genotypes, which were evaluated in a factorial completely randomized design with four replications, and seedling characteristics were measured; (b) A greenhouse experiment where the same statistical design as the lab study was used for seed germination and establishment of 19 sugar beet materials, with irrigation water EC= 3 and 16 dS m-1; and (c) A field experiment that was carried out to study the response of nine selected genotypes to irrigation waters with EC= 4 and EC= 16 dS m-1, using a split plot design with three replications. Interaction effects of salinity and genotypes were statistically significant (α= 0.01) for percentage of germination, abnormal seedling, and root and hypocotyls lengths. Indeed, sugar beet germination decreased to 35% and dead seedlings increased to 80 % under salinity stress (EC= 16 dS m-1) in the greenhouse. Genotypes were ranked from tolerant to susceptible. The results of field experiment were consistent with that obtained in the greenhouse. It can be concluded that salt stress decreased seed germination and, later on, crop establishment by increasing dead seedlings; consequently, sugar beet yield decreased. It seems that establishment is more susceptible to salinity than germination. Root length and abnormal seedling are good indexes for screening sugar beet genotypes for salinity tolerance at the primary growth stages.

Davarani F.H.,Islamic Azad University | Safarpour H.,Nanobiosensors Research Group | Safarpour H.,Pharmaceutical science Research Center | Safarnejad M.R.,Nanobiosensors Research Group | And 7 more authors.
Euphytica | Year: 2014

Identification of resistance resources of sugar beet germplasm against Polymyxa betae has always been a critical concern among sugar beet breeders. In the present study, two different methods including the DAS-enzyme-linked immunosorbent assay (ELISA) test and a nanobiosensor method based on florescent resonance transfer energy (FRET) were compared in order to achieve resistant germplasm. More specifically, 58 sugar beet germplasm as well as two negative and two positive controls were cultivated in infested soil under greenhouse conditions. The contamination level or in another words sensitivity and specificity observed based on the ELISA readings was inaccurately lower in comparison with the investigated nanobiosensor. Moreover, the nanobiosensor was 70 folds less time consuming compared to the ELISA method, for the immuno-reaction was much faster and no sample treatment steps were required. As a result, the quantum dots-FRET-based nanobiosensor investigated herein could well suit the task of everyday screening of resistance resource and could be efficiently used in breeding programs. © 2014, Springer Science+Business Media Dordrecht.

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