Sudhakarrao Naik Institute of Pharmacy

Pusad, India

Sudhakarrao Naik Institute of Pharmacy

Pusad, India
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Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
International Journal of PharmTech Research | Year: 2010

Alzheimer's disease (AD) is said to be the leading cause of dementia in elderly individuals exhibit a marked decline in cognitive functions. There are several factors involves in the pathogenesis of AD e.g. involvement of cholinesterase enzyme, oxidative free radicals and high cholesterol level promoting risk for and exacerbating AD pathology. In the light of this, the present study was undertaken to investigate the influence of ethanolic extracts (300 and 500mg/kg p.o.) of Murraya koenigii leaves (MKL) on memory in aged mice. The study was also undertaken to evaluate for cholinesterase and cholesterol level respectively. The result of the present study indicates that administration of MKL for 15 days produce significant dose dependant improvement of memory. The results also indicate to reduce the brain cholinesterase activity and total cholesterol levels respectively. Thus it concludes that the underlying mechanism of action for the observed nootropic effect may be attributed to anticholinesterase and a cholesterol lowering property of MKL. Therefore, it would be worthwhile to investigate specifically the therapeutic potential of MKL in the management of Alzheimer patients.


Tembhurne S.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.,Sudhakarrao Naik Institute of Pharmacy
Indian Journal of Pharmaceutical Sciences | Year: 2011

The aim of the present study was to study the effect of chronic treatment (9 weeks) of fluoxetine (20 mg/kg p.o.) a selective serotonin reuptake inhibitor on blood glucose level and in prevention of diabetic neuropathic pain perception. Evaluation of diabetic neuropathy was performed after 9 weeks of single injection of streptozotocin (70 mg/kg i.v.) in rats. Blood glucose level, glycated haemoglobin, grip strength, pain sensitivity and threshold in diabetic rats were measured at the end of 9 weeks. The results of the present study indicate that the 9 weeks treatment of fluoxetine demonstrates hypoglycemic effect; it marked decreases the blood glucose level in diabetic treated animals. There was also decrease in the grip strength in diabetic rat indicates to induction of neuropathy or nerve damage. Fluoxetine increase the grip strength of diabetic rats. There was also found loss of pain perception in diabetes rats which measured using hot plate and tail flick methods. Fluoxetine increases the licking time and withdrawal latency in hot plate and tail flick test respectively indicates the presence of pain perception and prevention of nerve damage demonstrates its protective effect in diabetic neuropathy. Our study concludes the chronic treatment of fluoxetine significantly decreases the glycemic level as well as it protected from the development of diabetic neuropathy.


Jadhav J.K.,Sudhakarrao Naik Institute of Pharmacy | Sreenivas S.A.,Guru Nanak Institute of Pharmacy
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

The purpose of this research work was to develop and evaluate matrix-type Transdermal drug delivery system containing Indomethacin with different ratios of hydrophilic and hydrophobic polymeric combinations by the solvent evaporation technique. The physicochemical compatibility of the drug and the polymers were studied by infrared spectroscopy. The results suggested no physicochemical incompatibility between the drug and the polymers. Transdermal patch formulations consists of Hydroxypropyl methylcellulose E5 and Ethyl cellulose in the ratios of 10:0, 0:10, 1:9, 2:8,3:7, 4:6, 5:5, 6:4, 7:3, 8:2 and 9:1 respectively were prepared. All formulations carried 20% v/w of dimethyl sulfoxide as penetration enhancer and 20% v/w of dibutyl phthalate as plasticizer in chloroform and methanol (1:1) as solvent system. The prepared Transdermal patches were evaluated for in vitro release, moisture absorption, moisture loss and mechanical properties. The diffusion studies were performed by using Franz diffusion cells. The formulation, F8 with combination of polymers (3:2) showed maximum release of 75.28% in 24 h. Hence, it can be reasonably concluded that Indomethacin can be formulated into the Transdermal matrix type patches to sustain its release characteristics.


Kshirsagar S.N.,Sudhakarrao Naik Institute of Pharmacy
International Journal of PharmTech Research | Year: 2011

Dementia is one of the age related mental problem and characteristic symptoms of various neurodegenerative disorder including Alzheimer's disease. Stressful conditions are often associated with loss of memory and other cognitive functions. Various drugs like diazepam, alcohol, barbiturates disrupt learning and memory. Traditionally herbal drugs have been used to enhance cognitive function. Present work was undertaken to assess the potential of dried seed kernels of Ceasalpinia crista extract as learning and memory enhancer. Aqueous extract of dried seed kernels of Ceasalpinia crista linn.ameliortated the amnesic effect of scopolamine in mice. Redial arm maze and Morries water maze paradigm served as the exteroceptive behavioral models. Aqueous extract of dried seed kernels of Caesalpinia crista linn. is compered with standered drug piracetam in scopolamine induced amnesia in mice. Aqueous extract Caesalpinia crista linn. could beneficial to improve cognition in disorders like dementia and various neurodegenerative disorders.


Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Alzheimer's disease (AD) is said to be the leading cause of dementia in elderly individuals exhibit a marked decline in cognitive functions. There are several factors involves in the pathogenesis of AD e.g. involvement of cholinesterase enzyme, oxidative free radicals and high cholesterol level promoting risk for and exacerbating AD pathology. In the light of this, the present study was undertaken to investigate the influence of petroleum ether extracts (300 and 500mg/kg p.o.) of Murraya koenigii leaves (MKL) on memory in aged mice. The study was also undertaken to evaluate for cholinesterase and cholesterol level. The result of the present study indicates that administration of MKL for 15 days produce significant dose dependant improvement of memory. The results also indicate to reduce the brain cholinesterase activity and total cholesterol levels respectively. Thus it concludes that the underlying mechanism of action for the observed nootropic effect may be attributed to anticholinesterase and a cholesterol lowering property of MKL. Therefore, it would be worthwhile to investigate specifically the therapeutic potential of MKL in the management of Alzheimer patients.


Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
Asian Pacific Journal of Tropical Disease | Year: 2012

Objective: To evaluate the hypoglycemic and anti-obesity activities of of Murraya koenigii leaves. Method: The study was performed in high fatty diet induced obesity rats. After 15 days baseline period the treatments animals were received ethanolic extract of Murraya koenigii leaves (300 and 500 mg/kg) in high fatty diet rats. All the treatments were given for one month. On 30th day all the fasted animals received an intraperitoneal injection of glucose (1 g/kg) for glucose tolerance test. At the end of study body weight, total cholesterol, triglycerides, and blood glucose level were measured. Results: The results demonstrate clearly that repeated oral administration of Murraya koenigii leaves evoked a potent anti-hyperglycaemic activity in high fat diet obese rats. Postprandial hyperglycaemic peaks were significantly lower in plant-treated experimental groups. In other hand, high fatty diet group increased the both total cholesterol and triglycerides levels as compared to control group. While administration of Murraya koenigii leaves significantly decreased in both cholesterol as well as triglycerides. Conclusions: We can conclude that Murraya koenigii leaves evokes potent anti-hyperglycaemic and anti-obesity effects. This fact could support their use by the diabetes patient for controlling body weight as well as maintains the glycemic level. © 2012 Asian Pacific Tropical Medicine Press.


Deshmukh V.N.,Sudhakarrao Naik Institute of Pharmacy
International Journal of PharmTech Research | Year: 2012

Oral delivery is currently the gold standard in the pharmaceutical industry where it is regarded as the safest, most convenient and most economical method of drug delivery having the highest patient compliance. Methods to improve patient's compliance have always attracted scientists towards the development of fancy oral drug delivery systems. Among them, mouth dissolving drug delivery systems (MDDDS) have acquired an important position in the market by overcoming previously encountered administration problems and contributing to extension of patent life. MDDDS have the unique property of rapidly disintegrating and/or dissolving and releasing the drug as soon as they come in contact with saliva, thus obviating the requirement of water during administration. Therefore, these dosage forms have lured the market for a certain section of the patient population which include dysphagic, bed ridden, psychic, geriatric and paediatric patients. Research in developing orally disintegrating systems has been aimed at investigating excipients as well as technique to meet these challenges. Technologies used for manufacturing of orally disintegrating tablets are either conventional technologies or patented technologies. In conventional freeze drying, tablet molding, sublimation, spray drying etc. and in patented Zydis technology, Orasolv technology, Durasolv technology, Wowtab technology, Flashdose technology are important. Important ingredients that are used in the formulation of ODTs should allow quick release of the drug, resulting in faster dissolution.


Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy
Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine | Year: 2011

The present study was to investigate the effects of Murraya koenigii leaf (MKL), an Indian herb, on glucose homeostasis, intestinal transit time, response to exogenous acetylcholine of smooth muscles of distal colon, and intestinal thiobarbituric acid reactive substance (TBARS) level in streptozotocin (STZ)-induced diabetic rats. Male adult Wistar rats were used in this study. Diabetes was induced in the rats by STZ (70 mg/kg, intravenously). The treatments of MKL extract (300 and 500 mg/kg) and glibenclamide were started after stabilization of blood glucose level (13 d after single dose of STZ), while the standard drug cisapride or vitamin E was given from the last week (8th week) of experimentation. At the end of the study, the rats were sacrificed and evaluated for gastrointestinal motility, the contractile response of distal colons and the TBARS content. The gastrointestinal motility was evaluated by measuring the intestinal transit rate of charcoal meal. The contractile response of distal colon was measured in terms of evaluating the dose-response curve with increasing doses of acetylcholine, and the TBARS content was measured by calculating the level of polyunsaturated fatty acid in homogenates of intestines of the diabetic rats. MKL significantly decreased the blood glucose level at the 30th (P<0.05) and 60th (P<0.01) day of MKL administration (300 and 500 mg/kg). The gastrointestinal motility significantly (P<0.05) reduced after 9 weeks in diabetic rats and it was correlated to the decrease of the percent response of acetylcholine on distal colons (P<0.01) and the increase of TBARS (as an index of oxidative stress) in intestines (P<0.05), while prior treatment with MKL (300 and 500 mg/kg) up to 9 weeks increased the gastrointestinal motility demonstrated by the increase in the activation of cholinergic response to acetylcholine on distal colons (P<0.05). The TBARS also decreased after 9-week treatment with MKL (P<0.05). The present study suggested that MKL had protective effect against gastrointestinal disturbances in diabetes by controlling glucose level as well as defending against peripheral damage of cholinergic neurons by providing antioxidant shelter, so it may be helpful in diabetic patients with impaired gastrointestinal motility.


Chauhan H.V.,Sudhakarrao Naik Institute of Pharmacy
Research Journal of Pharmacy and Technology | Year: 2011

Present in-vivo study was carried out to evaluate the antidiabetic, lipid lowering and antioxidant activity of methanolic extract of Lawsonia inermis leaves in alloxan induced diabetic rats using glibenclamide (10mg/kg) as standard drug. Oral administration of methanolic extract of Lawsonia inermis leaves (600mg/kg body weight) for 14 days resulted in significant decrease in fasting blood glucose level (34.33%), serum triglycerides (43.35%), total cholesterol (34.18%), low density lipoprotein (11.05%), GOT (42.89%), and GPT (28.09%) with simultaneous increase in serum high density lipoprotein (50.48%) and serum glucokinase (208.75%). Further the methanolic extract of Lawsonia inermis leaves inhibited the generation of Malondialdehyd (TBARS) level (34.34%) in alloxan induced diabetic rats. The results of the present study demonstrate antidiabetic, lipid lowering and antioxidant activities in methanolic extract of Lawsonia inermis leaves, which could help in prevention of diabetic hyperlipedemia and related diseases. © RJPT All right reserved.


Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
Journal of Medicinal Plants Research | Year: 2010

Diabetes causes increased oxidative stress, which is thought to play an important role in the pathogenesis of various diabetic complications. The antioxidant actions are keys to preventing or reversing diabetes and its complications. Thus the aim of the present study was to evaluate the antioxidant potential of ethanolic Murraya koenigii leaves (MKL) extract on Thiobarbituric Acid Reactive Substances (TBARS) as index of lipid peroxidation and on the glycemic control in streptozotocin induced diabetic rats. Effect of oral administration of MKL (300 and 500 mg/kg) on the level of blood glucose, glycosylated haemoglobin, on TBARS and glycogen levels were estimated in streptozotocin induced diabetic rats. Glibenclamide was used as a standard drug. The elevated level of blood glucose, glycosylated haemoglobin, TBARS observed in diabetic rats were significantly altered compared to control but it not come to normal level after treatment with MKL for 15 days in diabetic rats. From the results it suggest that the ethanolic extract of M. koenigii possessed potent antioxidant properties which may be due to the presence of biological active ingredient such as carbazole alkaloids, glycoside, triterpenoids and phenolic compounds. Thus the antidiabetic activity of M. koenigii leaves was probably due to the presence of its antioxidant property. © 2010 Academic Journals.

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