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Game M.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.,Sudhakarrao Naik Institute of Pharmacy
Indian Journal of Pharmaceutical Sciences | Year: 2011

Two simple, accurate and precise spectrophotometric methods have been developed for simultaneous determination of nitazoxanide and ofloxacin in tablets. Method I is Q-absorbance ratio method which involves Q-absorbance at isobestic point (306.25 nm) and max (347.5 nm) of nitazoxanide, while method II is two wavelength method, where 244.6 nm and 273.0 nm were selected as 1 and 2 for determination of nitazoxanide and 294.3 nm and 388.1 nm were selected as 3 and 4 for determination of ofloxacin. Both drugs obeyed the Beer′s law in the concentration range 2-30 g/ml,correlation coefficient (r 2 <1). Both methods were validated statistically and recovery studies were carried out to confirm the accuracy. Commercial tablet formulation was successfully analyzed using the developed methods.


Sarage R.D.,Sudhakarrao Naik Institute of Pharmacy
International Journal of Pharmacy and Technology | Year: 2012

The purpose of this research work was to develop a stable formulation of Antihypertensive drugs of the Telmisartan and Hydrochlorothiazide immediate release bilayer tablet and to study the dissolution profile with the reference product. The Formulation development work was initiated with wet granulation (drug layering technique) as the APIs are very static in nature and having very poor flow property. In order to improve solubility and drug release, Telmisartan is converted to its sodium salt by dissolving in aqueous solution of Sodium Hydroxide. Lactose Monohydrate and Microcrystalline Cellulose are used as diluents. Sodium Starch Glycolate is added as a superdisintegrant and Magnesium Stearate is used as the lubricant. The prepared granules are compressed into Double layer compression machine. The tablets thus formulated showed satisfactory physical parameters, and it was found to be stable and in-vitro release studies are shown that formulation (A5B5) shows Cumulative percent drug release 98.9-103.7% within 60min and matches with that of Innovator.


Chandan H.S.,Sudhakarrao Naik Institute of Pharmacy | Tapas A.R.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
International Journal of Phytomedicine | Year: 2011

Coriandrum sativum Linn. (CSL) is popularly known as coriander in India belongs to family Apiaceae. The objective of the present work was to evaluate the in-vitro anthelmintic potency of the ethanolic extract and carbon tetrachloride extract of Coriandrum sativum Linn. plant using Indian earthworms (Pheretima posthumad). The various concentrations (50, 100, and 150 mg/ml) of the ethanolic extract and carbon tetrachloride extract were tested in-vitro for anthelmintic potency by determination of time of paralysis and time of death of worm. Piperazine citrate (15mg/ml) used as standard. The result of present study indicates that the Coriandrum sativum Linn. potentiate to paralyze earthworm and also caused its death after some time. The shortest time of paralysis was observed at higher dose (150 mg/ml) of both ethanolic extract and carbon tetrachloride were found to 28 min and 45 min respectively. The results of the study are comparable to standard Piperazine citrate. The result showed that ethanolic extract of Coriandrum sativum Linn. took less time to cause paralysis of the earthworm than that of carbon tetrachloride extract Coriandrum sativum Linn. Thus the present study demonstrate that the Coriandrum sativum Linn. as an anthelmintic has been confirm as the ethanolic and carbon tetrachloride extracts of whole plant displayed activity against the earthworm used in study.


Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Alzheimer's disease (AD) is said to be the leading cause of dementia in elderly individuals exhibit a marked decline in cognitive functions. There are several factors involves in the pathogenesis of AD e.g. involvement of cholinesterase enzyme, oxidative free radicals and high cholesterol level promoting risk for and exacerbating AD pathology. In the light of this, the present study was undertaken to investigate the influence of petroleum ether extracts (300 and 500mg/kg p.o.) of Murraya koenigii leaves (MKL) on memory in aged mice. The study was also undertaken to evaluate for cholinesterase and cholesterol level. The result of the present study indicates that administration of MKL for 15 days produce significant dose dependant improvement of memory. The results also indicate to reduce the brain cholinesterase activity and total cholesterol levels respectively. Thus it concludes that the underlying mechanism of action for the observed nootropic effect may be attributed to anticholinesterase and a cholesterol lowering property of MKL. Therefore, it would be worthwhile to investigate specifically the therapeutic potential of MKL in the management of Alzheimer patients.


Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
Asian Pacific Journal of Tropical Disease | Year: 2012

Objective: To evaluate the hypoglycemic and anti-obesity activities of of Murraya koenigii leaves. Method: The study was performed in high fatty diet induced obesity rats. After 15 days baseline period the treatments animals were received ethanolic extract of Murraya koenigii leaves (300 and 500 mg/kg) in high fatty diet rats. All the treatments were given for one month. On 30th day all the fasted animals received an intraperitoneal injection of glucose (1 g/kg) for glucose tolerance test. At the end of study body weight, total cholesterol, triglycerides, and blood glucose level were measured. Results: The results demonstrate clearly that repeated oral administration of Murraya koenigii leaves evoked a potent anti-hyperglycaemic activity in high fat diet obese rats. Postprandial hyperglycaemic peaks were significantly lower in plant-treated experimental groups. In other hand, high fatty diet group increased the both total cholesterol and triglycerides levels as compared to control group. While administration of Murraya koenigii leaves significantly decreased in both cholesterol as well as triglycerides. Conclusions: We can conclude that Murraya koenigii leaves evokes potent anti-hyperglycaemic and anti-obesity effects. This fact could support their use by the diabetes patient for controlling body weight as well as maintains the glycemic level. © 2012 Asian Pacific Tropical Medicine Press.


Deshmukh V.N.,Sudhakarrao Naik Institute of Pharmacy
International Journal of PharmTech Research | Year: 2012

Oral delivery is currently the gold standard in the pharmaceutical industry where it is regarded as the safest, most convenient and most economical method of drug delivery having the highest patient compliance. Methods to improve patient's compliance have always attracted scientists towards the development of fancy oral drug delivery systems. Among them, mouth dissolving drug delivery systems (MDDDS) have acquired an important position in the market by overcoming previously encountered administration problems and contributing to extension of patent life. MDDDS have the unique property of rapidly disintegrating and/or dissolving and releasing the drug as soon as they come in contact with saliva, thus obviating the requirement of water during administration. Therefore, these dosage forms have lured the market for a certain section of the patient population which include dysphagic, bed ridden, psychic, geriatric and paediatric patients. Research in developing orally disintegrating systems has been aimed at investigating excipients as well as technique to meet these challenges. Technologies used for manufacturing of orally disintegrating tablets are either conventional technologies or patented technologies. In conventional freeze drying, tablet molding, sublimation, spray drying etc. and in patented Zydis technology, Orasolv technology, Durasolv technology, Wowtab technology, Flashdose technology are important. Important ingredients that are used in the formulation of ODTs should allow quick release of the drug, resulting in faster dissolution.


Dhurvey Y.R.,Sudhakarrao Naik Institute of Pharmacy | Kawtikwar P.S.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
International Journal of ChemTech Research | Year: 2012

Cow ghee was analyzed for physicochemical characteristics before and after hydrogenation for quality comparison, extension of shelf-life and for improving its palatability, acceptability. Concerning the least amount of catalyst used and least iodine value, hydrogenated cow ghee was prepared at 0.075% w/w catalyst, 0.86 MPa of pressure and 408 K of temperature. The Fourier transform infrared spectroscopy spectra showed the characteristic peak at 966/cm corresponding to trans compounds for cow ghee (CG) and hydrogenated cow ghee (HCG) examined. The results indicated the presence of trans fatty acids (TFA) in CG and HCG. Increase in specific gravity, melting range and decrease in solidification temperature of HCG indicates that an increase in solid fat content as compared to CG. Increase in acid value, decrease in ester value and saponification value indicates that an increase in free fatty acid content in HCG. Decrease in iodine value of HCG indicates that degree of unsaturation was reduced to a minimal level and conversion of unsaturated fatty acids to saturated fatty acids occurred. Hydroxyl value indicated the presence of hydroxyl group in CG and HCG. No significant increase in heavy metal content and nickel has been observed due to good laboratory practices. Improvement of traditional ghee processing parameters and quality properties are necessary, using good manufacturing practices on unit operation. In addition, proper packaging in containers injected with inert gases such as nitrogen and addition of antioxidants for storage and distribution will be an advantage.


Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy
Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine | Year: 2011

The present study was to investigate the effects of Murraya koenigii leaf (MKL), an Indian herb, on glucose homeostasis, intestinal transit time, response to exogenous acetylcholine of smooth muscles of distal colon, and intestinal thiobarbituric acid reactive substance (TBARS) level in streptozotocin (STZ)-induced diabetic rats. Male adult Wistar rats were used in this study. Diabetes was induced in the rats by STZ (70 mg/kg, intravenously). The treatments of MKL extract (300 and 500 mg/kg) and glibenclamide were started after stabilization of blood glucose level (13 d after single dose of STZ), while the standard drug cisapride or vitamin E was given from the last week (8th week) of experimentation. At the end of the study, the rats were sacrificed and evaluated for gastrointestinal motility, the contractile response of distal colons and the TBARS content. The gastrointestinal motility was evaluated by measuring the intestinal transit rate of charcoal meal. The contractile response of distal colon was measured in terms of evaluating the dose-response curve with increasing doses of acetylcholine, and the TBARS content was measured by calculating the level of polyunsaturated fatty acid in homogenates of intestines of the diabetic rats. MKL significantly decreased the blood glucose level at the 30th (P<0.05) and 60th (P<0.01) day of MKL administration (300 and 500 mg/kg). The gastrointestinal motility significantly (P<0.05) reduced after 9 weeks in diabetic rats and it was correlated to the decrease of the percent response of acetylcholine on distal colons (P<0.01) and the increase of TBARS (as an index of oxidative stress) in intestines (P<0.05), while prior treatment with MKL (300 and 500 mg/kg) up to 9 weeks increased the gastrointestinal motility demonstrated by the increase in the activation of cholinergic response to acetylcholine on distal colons (P<0.05). The TBARS also decreased after 9-week treatment with MKL (P<0.05). The present study suggested that MKL had protective effect against gastrointestinal disturbances in diabetes by controlling glucose level as well as defending against peripheral damage of cholinergic neurons by providing antioxidant shelter, so it may be helpful in diabetic patients with impaired gastrointestinal motility.


Chauhan H.V.,Sudhakarrao Naik Institute of Pharmacy
Research Journal of Pharmacy and Technology | Year: 2011

Present in-vivo study was carried out to evaluate the antidiabetic, lipid lowering and antioxidant activity of methanolic extract of Lawsonia inermis leaves in alloxan induced diabetic rats using glibenclamide (10mg/kg) as standard drug. Oral administration of methanolic extract of Lawsonia inermis leaves (600mg/kg body weight) for 14 days resulted in significant decrease in fasting blood glucose level (34.33%), serum triglycerides (43.35%), total cholesterol (34.18%), low density lipoprotein (11.05%), GOT (42.89%), and GPT (28.09%) with simultaneous increase in serum high density lipoprotein (50.48%) and serum glucokinase (208.75%). Further the methanolic extract of Lawsonia inermis leaves inhibited the generation of Malondialdehyd (TBARS) level (34.34%) in alloxan induced diabetic rats. The results of the present study demonstrate antidiabetic, lipid lowering and antioxidant activities in methanolic extract of Lawsonia inermis leaves, which could help in prevention of diabetic hyperlipedemia and related diseases. © RJPT All right reserved.


Tembhurne S.V.,Sudhakarrao Naik Institute of Pharmacy | Sakarkar D.M.,Sudhakarrao Naik Institute of Pharmacy
Journal of Medicinal Plants Research | Year: 2010

Diabetes causes increased oxidative stress, which is thought to play an important role in the pathogenesis of various diabetic complications. The antioxidant actions are keys to preventing or reversing diabetes and its complications. Thus the aim of the present study was to evaluate the antioxidant potential of ethanolic Murraya koenigii leaves (MKL) extract on Thiobarbituric Acid Reactive Substances (TBARS) as index of lipid peroxidation and on the glycemic control in streptozotocin induced diabetic rats. Effect of oral administration of MKL (300 and 500 mg/kg) on the level of blood glucose, glycosylated haemoglobin, on TBARS and glycogen levels were estimated in streptozotocin induced diabetic rats. Glibenclamide was used as a standard drug. The elevated level of blood glucose, glycosylated haemoglobin, TBARS observed in diabetic rats were significantly altered compared to control but it not come to normal level after treatment with MKL for 15 days in diabetic rats. From the results it suggest that the ethanolic extract of M. koenigii possessed potent antioxidant properties which may be due to the presence of biological active ingredient such as carbazole alkaloids, glycoside, triterpenoids and phenolic compounds. Thus the antidiabetic activity of M. koenigii leaves was probably due to the presence of its antioxidant property. © 2010 Academic Journals.

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