Subei Peoples Hospital Of Jiangsu Province
Subei Peoples Hospital Of Jiangsu Province
Luan N.,Nanjing Agricultural University |
Zhou C.,Nanjing Agricultural University |
Li P.,Nanjing Agricultural University |
Ombati R.,CAS Kunming Institute of Zoology |
And 10 more authors.
Thrombosis and Haemostasis | Year: 2017
The repugnatorial glands of millipedes release various defensive chemical secretions. Although varieties of such defensive secretions have been studied, none of them is protein or peptide. Herein, a novel factor Xa (FXa) inhibitor named joannsin was identified and characterised from repugnatorial glands of Prospirobolus joannsi. Joannsin is composed of 72 amino acid residues including six cysteines, which form three intra-molecular disulfide bridges. It is a member of Kunitztype protease inhibitor family, members of which are also found in the secretory glands of other arthropods. Recombinant joannsin exhibited remarkable inhibitory activity against trypsin and FXa with a Ki of 182.7 ± 14.6 and 29.5 ± 4.7 nM, respectively. Joannsin showed strong anti-thrombosis functions in vitro and in vivo. Joannsin is the first peptide component in millipede repugnatorial glands to be identified and is a potential candidate and/or template for the development of antithrombotic agents. These results also indicated that there is Kunitztype protease inhibitor toxin in millipede repugnatorial glands as in other arthropods secretory glands. © Schattauer 2017.
Sun Y.,West Virginia University |
Sun Y.,Subei Peoples Hospital Of Jiangsu Province |
Wang T.L.,West Virginia University |
Toh W.S.,National University of Singapore |
Pei M.,West Virginia University
European Cells and Materials | Year: 2017
As a key molecule of extracellular matrix, laminin provides a delicate microenvironment for cell functions. Recent findings suggest that laminins expressed by cartilage-forming cells (chondrocytes, progenitor cells and stem cells) could promote chondrogenesis. However, few papers outline the effect of laminins on providing a favorable matrix microenvironment for cartilage regeneration. In this review, we delineated the expression of laminins in hyaline cartilage, fibrocartilage and cartilage-like tissue (nucleus pulposus) throughout several developmental stages. We also examined the effect of laminins on the biological activities of chondrocytes, including adhesion, migration and survival. Furthermore, we scrutinized the potential influence of various laminin isoforms on cartilage-forming cells’ proliferation and chondrogenic differentiation. With this information, we hope to facilitate an understanding of the spatial and temporal interactions between cartilage-forming cells and laminin microenvironment to eventually advance cell-based cartilage engineering and regeneration. © 2017, AO Research Institute. All rights reserved.
Wang D.,Nanjing Medical University |
Chen H.,Wuxi Peoples Hospital |
Wang S.,Nanjing Medical University |
Zou Y.,Wuxi Peoples Hospital |
And 8 more authors.
Clinical Rheumatology | Year: 2016
Thalidomide is effective for treating severe cutaneous lupus patients. The aim of this study was to observe the optimum effective and maintenance doses of thalidomide to maximize clinical benefit and minimize side effects for patients with cutaneous lupus in China. Sixty-nine patients with lupus rash from eight hospitals in China were enrolled and treated with different doses of thalidomide. We started the dose of thalidomide at 25 mg daily and gradually increased administration dose once a week until erythema was markedly improved. The effective and maintenance doses were documented. The size of skin lesions was noted once a week. Systemic lupus erythematosus disease activity index (SLEDAI) score, levels of erythrocyte sedimentation rate (ESR), and serum TNF-α were measured before and after treatment. The remission rates were evaluated weekly until 8 weeks. Sixty-eight percent of patients showed an effective dose of 50 mg daily; another 13, 10, and 9 % of patients had an effective dose of 100, 75, and 25 mg daily, respectively. The maintenance dose was 50 mg daily for 71 % of the patients, and 100, 75, and 25 mg daily for 9, 14, and 6 % of the patients. SLEDAI score and serum ESR levels significantly decreased 4 weeks after thalidomide treatment. At the end of the fourth week, the rates of complete remission, partial remission, and no response were 56 % (n = 39), 41 % (n = 28), and 3 % (n = 2). At the eighth week, the rate of total remission rose up to 100 %. The most common side effects were drowsiness and constipation. No peripheral neuropathy was observed in these patients. Thalidomide at a dose of 50 mg daily may offer a better benefit to risk ratio in the treatment of Chinese cutaneous lupus patients. © 2016, International League of Associations for Rheumatology (ILAR).
Chen L.,Subei Peoples Hospital Of Jiangsu Province |
Zhao W.,Subei Peoples Hospital Of Jiangsu Province |
Wang L.,Subei Peoples Hospital Of Jiangsu Province |
Gao S.-J.,Subei Peoples Hospital Of Jiangsu Province |
And 4 more authors.
World Chinese Journal of Digestology | Year: 2015
AIM: To evalute the efficacy and safety of peroral endoscopic myotomy (POEM) for esophageal achalasia (AC), submucosal tunnel endoscopic resection (STER) for esophageal submucosal tumors (SMT) originating from muscularis propria layer and endoscopic submucosal tunnel dissection (ESTD) for large esophageal superficial neoplasms. METHODS: A retrospective study was performed of the clinical data of 10 patients with esophageal achalasia treated by POEM, 18 patients with esophageal submucosal tumors originating from muscularis propria layer treated by STER, and 22 patients with large esophageal superficial neoplasms treated by ESTD at Department of Gastroenterology, Subei People’s Hospital of Jiangsu Province from September 2012 to January 2015. Intraoperative and postoperative complications and clinical efficacy were recorded.RESULTS: The surgeries were successful in all patients. Subcutaneous emphysema occurred in three patients treated by POEM and eight patients by STER, and mediastinal emphysema occurred in one patient treated by POEM. In the 10 cases of POEM,the mean submucosal tunnel length was 10.1 cm ± 2.7 cm, the average length of endoscopic myotomy was 8.4 cm ± 1.9 cm, and the mean operation time was 72.5 min ± 29.1 min. Dysphagia symptom was improved significantly during 6 mo of the followup. In the 18 cases of STER, the average lesion size was 1.5 cm ± 0.6 cm, the average operation time was 52.3 min ± 21.4 min, and postoperative pathological examination revealed that all were leiomyomas. Postoperative follow-up duration ranged from 3 to 6 mo and no residual lesion or recurrence was found. In the 22 cases of ESTD, the length of lesions ranged from 2.5 cm to 6.0 cm and the width from 1/3 to 4/5 of the esophageal circumference. The average operation time was 82.9 min ± 26.5 min. Postoperative pathology was suggestive of 16 cases of high-grade intraepithelial neoplasia and 6 cases of intramucosal carcinoma. During 6 mo of follow-up there was no recurrence. Four patients had postoperative esophageal strictures and were alleviated by pneumatic balloon dilatation and retrievable esophageal stent implantation. CONCLUSION: Submucosal tunnel endoscopy is a safe and effective method for rectal esophageal achalasia, esophageal submucosal tumors originating from the muscularis propria layer and large esophageal superficial neoplasms. © 2015 Baishideng Publishing Group Inc. All rights reserved.
Xiong Q.-Q.,Central South University |
Wang D.-R.,Subei Peoples Hospital Of Jiangsu Province |
Liang Y.,Yangzhou University
World Chinese Journal of Digestology | Year: 2016
The liver is the main target organ of metastasis for gastric cancer. Gastric cancer with liver metastases (GCLM) has a poor prognosis and is a challenge for treatment of patients with gastric cancer. At present the mechanism of liver metastases of gastric cancer is unclear, and there have been no unified treatment guidelines. This paper mainly discusses the molecular mechanism and comprehensive therapy of liver metastases of gastric cancer. © 2016 Baishideng Publishing Group Inc. All rights reserved.
Shu Y.-S.,Subei Peoples Hospital Of Jiangsu Province |
Shu Y.-S.,Hangzhou First Peoples Hospital |
Tao W.,Subei Peoples Hospital Of Jiangsu Province |
Tao W.,Yangzhou East Hospital |
And 4 more authors.
Inflammation | Year: 2014
Galangin, an active ingredient of Alpinia galangal, has been shown to possess anti-inflammatory and antioxidant activities. Inflammation and oxidative stress are known to play vital effect in the pathogenesis of acute lung injury (ALI). In this study, we determined whether galangin exerts lung protection in lipopolysaccharide (LPS)-induced ALI. Male BALB/c mice were randomized to receive galangin or vehicle intraperitoneal injection 3 h after LPS challenge. Samples were harvested 24 h post LPS administration. Galangin administration decreased biochemical parameters of oxidative stress and inflammation, and improved oxygenation and lung edema in a dose-dependent manner. These protective effects of galangin were associated with inhibition of nuclear factor (NF)-κB and upregulation of heme oxygenase (HO)-1. Galangin reduces LPS-induced ALI by inhibition of inflammation and oxidative stress. © 2014, Springer Science+Business Media New York.
LI J.,Nanjing University of Aeronautics and Astronautics |
TANG X.-B.,Nanjing University of Aeronautics and Astronautics |
ZHANG X.-Z.,Subei Peoples Hospital Of Jiangsu Province |
GUI L.-G.,Subei Peoples Hospital Of Jiangsu Province |
And 3 more authors.
Journal of Mechanics in Medicine and Biology | Year: 2016
Purpose This study aimed to determine the dosimetric effect on the target volume, organs at risk (OARs) and normal tissues based on the different choice for four types of mechanical variables, i.e., treatment position, dose calculation algorithm, mulitleaf collimator (MLC) motion mode and X-ray energy; and to investigate the optimum treatment mode applied to post-operative cervical cancer for 5-field intensity-modulated radiation therapy (5F-IMRT) technique. Methods The dosimetric difference on the target volume and OARs under the influence of four types of variables were initially compared by changing one variable at a time. Then, based on the above compared results, we compared the dosimetric difference on planning target volume (PTV) and OARs between group A composed of the superior four variables and group B composed of the relatively inferior four variables. The dosimetric parameters included dose distribution of the target volume, OARs and normal tissues, conformal index (CI), homogeneity index (HI), monitor units (MU) and beam-on time ((Formula presented.). The independent and paired t-tests were used for statistical analysis, and the threshold for statistical significance was (Formula presented.). Results Compared with the supine position, the maximum dose of PTV ((Formula presented.)), the maximum dose of small intestine ((Formula presented.)) and (Formula presented.) of bladder ((Formula presented.) were all lower in prone position. In contrast with the pencil beam convolution (PBC), CI of PTV (CI(Formula presented.)) was larger while HI of PTV (HI(Formula presented.)) was lower, both (Formula presented.) and the maximum dose of rectum ((Formula presented.)) were lower using anisotropic analytical algorithm (AAA). Moreover, the same results were obtained using sliding window (SW) compared with multiple static segments (MSS). The mean dose of PTV ((Formula presented.) and CI(Formula presented.) was larger while the maximum dose of the spinal cord ((Formula presented.)), (Formula presented.) and the maximum dose of femoral heads were lower with 15 MV X-rays compared with 6 MV X-rays. In comparison with group B comprising the supine position, PBC, MSS and 6 MV X-rays, (Formula presented.) and HI(Formula presented.) decreased 1.4% and 53.4% respectively, CI(Formula presented.) increased 5.8% medially, while (Formula presented.), (Formula presented.), (Formula presented.) and (Formula presented.) all decreased in group A comprising of prone position, AAA, SW and 15 MV X-rays. Conclusion The treatment mode composed of prone position, AAA algorithm, SW and 15 MV X-rays is chosen for the post-operative cervical cancer of 5F-IMRT technique, which is more capable of meeting the target volume constraints and maximal protection of OARs. © 2016 World Scientific Publishing Company
Lian H.-B.,Subei Peoples Hospital Of Jiangsu Province |
Huang J.-H.,Subei Peoples Hospital Of Jiangsu Province |
Li P.-S.,Subei Peoples Hospital Of Jiangsu Province |
Xu G.,Subei Peoples Hospital Of Jiangsu Province |
And 5 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Previously, a rolling dermatome was often used to take skin tissues from the head, which demands a higher experience for an operator. Lack of experience easily leads to too thick skin tissues and impact hair growth in patients, and harvested skin tissues have a jagged edge that influences the enough utilization of harvested skin tissues and donor site. OBJECTIVE: To explore the clinical effects of air dermatome combined with infusion tumescent technique versus rolling dermatome combined with infusion tumescent technique to take the skin from the head. METHODS: Clinical data from 37 patients with severe burn undergoing air dermatome combined with infusion tumescent technique (experimental group) and 38 patients with severe burn undergoing rolling dermatome combined with infusion tumescent technique (control group) were compared. Both of the two groups underwent razor-thin graft (skin thickness was about 0.3 mm). The time of harvesting skin grafts, skin size, whether the skin grafts had uniform thickness and clean edges, healing time of skin donor sites, and survival rate of skin grafts were statistically analyzed in the two groups. RESULTS AND CONCLUSION: Infusion tumescent technique made the operation easy. Intraoperative and postoperative bleeding was significantly reduced. The mean time for harvesting skin grafts was 7.9 minutes in the experimental group, which was 3 minutes shorter than that in the control group (P < 0.05). In the experimental group, the skin grafts were larger with clean edges and uniform thickness, and the maximum graft was 12 cm×5 cm in size; all the donor sites were primarily healed, and the average healing time was 5.5 days, which was 2.2 days shorter than that in the control group (P < 0.05). All the donor sites in the two groups were primarily healed with no scalp infection, small scars or hair loss. Results from this study show that the air dermatome combined with infusion tumescent technique is a simple and effective method for harvesting skin grafts from the head and it has the remarkable superiority and important clinical value in extremely severe burn patients. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Chen W.-M.,Nanjing Medical University |
Chen W.-M.,Jining No 1 Peoples Hospital |
Huang M.-D.,Nanjing Medical University |
Kong R.,Subei Peoples Hospital Of Jiangsu Province |
And 6 more authors.
Digestive Diseases and Sciences | Year: 2015
Background: Long noncoding RNAs (lncRNAs) have been recently shown to play important regulatory roles in fundamental biological processes, and many of them are deregulated in several human cancers. LncRNA hypoxia-inducible factor 1alpha antisense RNA-2 (HIF1A-AS2) is overexpressed in nonpapillary clear-cell renal carcinomas and involved in cancer progression. Aim: This study was to evaluate the expression of HIF1A-AS2 in gastric cancer (GC) and further explore its biological function in GC cells. Materials and Methods: Quantitative real-time polymerase chain reaction was used to detect the expression level of HIF1A-AS2 in GC tissues. The correlation of its expression with clinicopathological features was analyzed. Area under receiver operating characteristic curve (ROCAUC) was constructed to evaluate the diagnostic value of HIF1A-AS2. Besides, tumor cell proliferation was assessed following knockdown of HIF1A-AS2, by MTT and colony formation assay in vitro, and tumor formation assay in a nude mouse model in vivo. Results: The expression of HIF1A-AS2 was upregulated in GC tumorous tissues compared with the adjacent normal tissues (P < 0.001). Its overexpression was correlated with TNM stages (P = 0.008), tumor invasion (P = 0.016), lymph node metastasis (P = 0.042), and poor prognosis (P = 0.001). In addition, ROCAUC of HIF1A-AS2 was up to 0.673 (95 % CI 0.596–0.744, P < 0.001). Moreover, knockdown of HIF1A-AS2 expression by siRNA could inhibit cell proliferation in vitro and tumorigenesis in vivo. Conclusions: HIF1A-AS2 is overexpressed in GC and may play a pivotal role in tumor cell proliferation. It can be used as a potential diagnostic and prognostic biomarker for GC. © 2015, Springer Science+Business Media New York.
Ge Y.-L.,Subei Peoples Hospital Of Jiangsu Province |
Li X.,Subei Peoples Hospital Of Jiangsu Province |
Gao J.,Subei Peoples Hospital Of Jiangsu Province |
Zhang X.,Subei Peoples Hospital Of Jiangsu Province |
And 4 more authors.
Experimental and Therapeutic Medicine | Year: 2016
The present study aimed to investigate the effects of dexmedetomidine (DEX) on cognition following a carotid endarterectomy (CEA). In addition, the neuroprotective effects of DEX against ischemia-reperfusion injury during CEA were analyzed. Patients due to undergo elective CEA under general anesthesia were randomly assigned to either the DEX-treated group (group D; n=25) or the control group (group C; n=25). Patients in group D were treated with 0.3 μg/kg DEX pre-CEA, followed by 0.3 μg/kg/h DEX intraoperatively up to 30 min prior to the completion of surgery, and the patients in group C received an equal volume of normal saline. Cognitive function was assessed prior to CEA (T0), and at 24, 48, and 72 h, 7 days and 1 month post-surgery (T1-5, respectively), using the Mini-Mental State Examination (MMSE). Blood samples were drawn from the ipsilateral jugular bulb of all patients at 20 min prior to anesthesia (t0), and at 10 min following tracheal intubation, 15 min following clamping and unclamping of the carotid artery, and at 6 and 24 h postoperatively (t1-5, respectively). The protein expression levels of markers of cerebral ischemia and injury, namely S100 calcium-binding protein B (S100B) and neuron-specific enolase (NSE), and the concentration of the oxidative stress marker malondialdehyde (MDA), were analyzed. Patients in group D exhibited elevated MMSE scores at T2 and T3 post-CEA, as compared with group C. Furthermore, the protein expression level of S100B and the concentration of MDA in the jugular bulb of group D patients were markedly decreased compared with those in group C at t3-5 and t3, respectively. The results of the present study suggested that DEX was able to enhance the recovery of cognition following CEA, and this was associated with decreased cerebral damage and antioxidative effects. © 2016, Spandidos Publications. All Rights reserved.