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Kadi S.,Subbaiah Institute of Medical science | Manjunath S.,International Medical School
Biomedicine (India) | Year: 2012

Background & Objectives: To determine oxidative stress by estimating blood malondialdehyde (MDA) levels and to assess the non-enzymatic antioxidant status by measuring plasma vitamin A, vitamin E and vitamin C levels in Rheumatoid arthritis (RA) patients and then comparing with the healthy individuals. Methods: Forty (40) subjects served as controls and 40 RA patients served as cases. The controls and cases were from both sexes and age group of 40-60 years. The parameters were estimated by standard biochemical methods. Results: The mean level of blood MDA (in nmol/ml) in controls was 6.19 ± 0.96 and 11.48 ± 0.76 in cases. The blood levels of MDA in RA cases were significantly increased as compared to controls (p<0.0001). The mean values of vitamin A, vitamin E and vitamin C in controls were 35.13+5.25 μg/dl, 0.92+0.10 mg/dl and 0.88+0.12 mg/dl respectively. The mean values of vitamin A, vitamin E and vitamin C in cases were 11.60 + 3.03 μg/dl, 0.53 + 0.14 mg/dl and 0.48 + 0.09 mg/dl respectively. A highly significant decrease in plasma levels of vitamin A, vitamin E and vitamin C was observed in RA cases as compared to controls(p<0.0001). Interpretation & Conclusion: The study concludes that there is an increased oxidative stress and a decreased antioxidant defense in patients with rheumatoid arthritis as evidenced by increased lipid peroxidation and low antioxidant levels. Source

Havanur B.,Bangalore Medical College and Research Institute | Raghavendra R.,Subbaiah Institute of Medical science | Pradeep Kumar M.P.,Bangalore Medical College and Research Institute
Medico-Legal Update | Year: 2016

Most of the undergraduates in medical schools are unaware of the importance of medico legal autopsy. Once the student are admitted to second year the student, many a times are in a dilemma why autopsies are to be witnessed by them at that point of time and are ignorant of the legal procedure which imbibes the medical professional to conduct autopsy in many cases of un-natural deaths where the cause of death is not known. Once the medical undergraduate gets the awareness regarding the Medico legal autopsy or forensic autopsy he has a different approach towards it. One such study was conducted at Bengaluru involving 200 students of 5th term on voluntary basis, who had ample knowledge regarding medico legal autopsy. A set of questionnaires were given to them and results tabulated and was concluded. This study shows the emotional reaction of the students and it indicates that the necropsy is of value in medical education. © 2016, World Informations Syndicate. All rights reserved. Source

Sambu N.K.,RIMS | Kashinath R.T.,Subbaiah Institute of Medical science | Ambekar J.G.,Hospital and Research Center
Journal of Clinical and Diagnostic Research | Year: 2015

Background: Diabetes Mellitus is a chronic metabolic disorder which may lead to various complications, the important being dyslipidemia leading to Coronary Heart Disorders (CHD), the major cause for morbidity and mortality in diabetic patients. Diabetes Mellitus could be treated by nutritional therapy/drug therapy and others. But the drug therapy would have its own limitations and side effects. To overcome from this an herbal extract is recommended, such as Diallyl Disulphide (DADS) a principle compound of Garlic oil. Aim: To assess the hypolipidemic effect of Diallyl Disulphide (DADS) in alloxan induced diabetic rats. Materials and Methods: Healthy adult wistar strain male albino rats weighing around 100-150 grams were randomly selected from the animal house at BLDE University’s Shri B.M.Patil Medical College, Hospital and Research Centre, Bijapur, India. Diabetes was induced using alloxan and was treated with DADS. After a stipulated time the rats were anesthetised and sacrificed to collect the blood and liver tissue. Various Lipid parameters, HMG CoA Reductase, Fecal bile acids were estimated in the blood, feces and homogenised liver tissue using standard procedures. Statiscal Analysis: One-way ANOVA followed by post-hoc t-test is done. Result: There was significant decrease in the blood and liver tissue lipid parameters of DADS treated alloxan induced diabetic rats when compared to the alloxan induced diabetic rats. Conclusion: From this study it can be concluded that the DADS a principle compound of garlic, definitely has the hypolipidemic effect in diabetic rats, which is reducing the morbidity in diabetic cases due to dyslipidemia without the adverse effects. © 2015, Journal of Clinical and Diagnostic Research. All rights reserved. Source

Pyati A.K.,Belagavi Institute of Medical science | Halappa C.K.,Subbaiah Institute of Medical science | Pyati S.A.,Navodaya Dental College | Nagaraj,Raichur Institute of Medical science | Wali V.,M R Medical College
Journal of Clinical and Diagnostic Research | Year: 2015

Background: The diagnostic accuracy of currently available standard panel of liver function tests is not satisfactory for the reliable diagnosis of chronic liver disorders. Earlier studies have reported that serum basal paraoxonase 1 (PON1) activity measurement may add a significant contribution to the liver function tests. Aim: To assess whether the measurement of serum basal paraoxonase 1 (PON1) activity would be useful as an index of liver function status in chronic hepatitis patients. Materials and Methods: The study included 50 chronic hepatitis patients and 50 apparently healthy controls based on inclusion & exclusion criteria. In all the subjects, standard liver function tests were analysed by using standard methods. Basal PON1 activity was estimated using spectrophotometric method by the hydrolysis of p-nitrophenylacetate. Student t-test, Pearson’s correlation coefficient, diagnostic validity tests and ROC curve analysis were the methods used for the statistical analysis of the data. Results: The serum basal PON1 activity was significantly decreased in chronic hepatitis cases when compared to controls (p< 0.001). Also basal PON1 activity was positively correlated with serum total protein and albumin, and negatively correlated with serum total bilirubin, alanine amino transferase (ALT), and alkaline phosphatase (ALP) (p< 0.001) in chronic hepatitis cases but not in healthy controls. Diagnostic validity tests showed, basal PON1 activity was a better discriminator of chronic hepatitis than total protein, albumin and ALP with sensitivity of 68%, specificity of 100%, positive predictive value of 100% and negative predictive value of 75%. ROC curve analysis demonstrated highest diagnostic accuracy for ALT (AUC = 0.999) followed by PON1 (AUC = 0.990), total bilirubin (AUC = 0.977), ALP (AUC = 0.904), total protein (AUC = 0.790) and albumin (AUC = 0.595). Conclusion: Diagnostic accuracy of serum PON1 activity is better than total bilirubin, total protein, albumin and ALP. PON1 activity measurement could significantly improve the current efficiency of a laboratory’s evaluation of patients with suspected chronic hepatitis. © 2015 Journal of Clinical and Diagnostic Research All rights reserved. Source

Channabasappa S.,SSS Mansion | Dharmappa S.,Subbaiah Institute of Dental science | Pandurangi R.,Subbaiah Institute of Medical science
Saudi Journal of Anaesthesia | Year: 2016

A 48-year-old male patient with a long-standing history of ankylosing spondylitis (AS) presented for ureteroscopic stone removal. On preoperative assessment, tracheal intubation was likely to be difficult due to decreased cervical spine mobility. Traditional neuraxial block was impossible due to the fusion of vertebral bodies. AS patients present the most serious array of intubation, which is secondary to decrease in cervical spine mobility and possible temporomandibular joint disease. Management of a case of AS can be very challenging when the airway and the central neuraxial blockade, both are difficult. Fluoroscopic assisted central neuraxial blockade may lead to predictable success in AS. We present a case report with severe AS where conventional techniques failed and C-arm assisted helped in successful epidural anesthesia for ureteroscopic stone removal. © 2016 Saudi Journal of Anesthesia. Source

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