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Stud Research Committee

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Shokrzadeh M.,Mazandaran University of Medical Sciences | Ahmadi A.,Mazandaran University of Medical Sciences | Chabra A.,Stud Research Committee | Naghshvar F.,University of Sfax | And 2 more authors.
Pharmaceutical Biology | Year: 2014

Context: Injury to normal tissues is the major limiting side effect of using cyclophosphamide (CP), an antineoplastic alkylating compound.Objective: This study was undertaken to evaluate the protective effect of an extract of Origanum vulgare L. (Lamiaceae), an antioxidative medicinal plant, against CP-induced oxidative lung damage in mice.Materials and methods: Mice were pre-treated with various doses of O. Vulgare extract (50, 100, 200, and 400 mg/kg) for 7 consecutive days followed by an injection with CP (200 mg/kg B.W.) One hour after the injection of O. vulgare on the last day, mice were injected with CP; 24 h later, they were euthanized, their lungs were immediately removed, and biochemical and histological studies were conducted.Results: A single dose of CP markedly altered the levels of several biomarkers associated with oxidative stress in lung homogenates. Pretreatment with O. vulgare significantly reduced the levels of lipid peroxidation and attenuated the alterations in glutathione content and superoxide dismutase activity induced by CP in lung tissue. In addition, O. vulgare effectively alleviated CP-induced histopathological changes in lung tissue.Conclusions: Our results revealed that O. vulgare protects lung tissues from CP-induced pulmonary damage and suggest a role for oxidative stress in the pathogenesis of lung disease produced by CP. Because O. vulgare has been extensively used as an additive agent and is regarded as safe, it may be used concomitantly as a supplement for reducing lung damage in patients undergoing chemotherapy. © 2014 Informa Healthcare USA.

Norouzabad L.M.,Stud Research Committee | Abed R.,Stud Research Committee | Sadat B.E.,University of Sfax | Jafarabadi M.A.,Tabriz University of Medical Sciences
Current Topics in Nutraceutical Research | Year: 2014

This study was designed to examine the effect of Burdock root tea on clinical signs and symptoms in patients with knee osteoarthritis (OA). Thirty-six patients (10 men and 26 women) aged 50-70 year-old with knee osteoarthritis referred to the physical medicine and rehabilitation department of the Tabriz University of Medical sciences Hospitals in 2013, were selected for the study and divided into two groups randomly. For all individuals along the 42 days of study period, the same drug treatments were considered. The intervention group received daily 3 cups of Burdock root tea (each cups contain 2gr/150 cc boiled water) half-hour after the meal. Control group received 3 cups contain 150 cc boiled water daily. The Knee Injury and Osteoarthritis Outcome Score (KOOS) Questionnaire, Timed Up and Go (TUG) and Visual Analog Scale (VAS) tests were used for clinical assessments. There was significant difference in pain intensity (P<0.001), scores of the KOOS Questionnaire (P=0.020) and TUG (P = 0.027) between the two groups after treatment. Significant reduction in pain intensity (p<0.001), significant decrease in the mean score of TUG (P<0.001) and significant increase in the mean score of KOOS Questionnaire (P<0.001) was noted in Burdock root tea group. © 2014 by New Century Health Publishers, LLC.

Aalaei S.,Stud Research Committee | Shahraki H.,Birjand University | Abu-Hanna A.,University of Amsterdam | Eslami S.,Stud Research Committee | Eslami S.,Pharmaceutical Research Center
Studies in Health Technology and Informatics | Year: 2014

Storage and transportation of red blood cells (RBCs) outside the standard temperature range as defined by guidelines can lead to hemolysis. One of the main factors believed to cause hemolysis is temperature.Infusion of the corrupted RBCs leads to haemolytic reactions which are severe and life-threatening. We developed a temperature monitoring system to monitor temperature changes of each blood bag during storage and transportation. The main objective of the present study was evaluating the accuracy of the temperature monitoring system and studying its feasibility. Validating the system relied on accurate digital thermometers that latch on a blood bag. To evaluate the feasibility, a case study was performed on 20 RBC bags transported from hospital blood bank to the cardiac surgery intensive care unit and the heart operating room. The results indicated that 12% of 25605 recorded temperatures (per minute) were outside the standard range. Minimum and maximum temperatures were 0.5°C and 16°C that were below and above the standard, respectively. The system was shown to be easily handled by users. The system is capable to alarm when a blood bag's temperature is outside the standard temperature and prevents blood corruption. This system can be used as a decision support system in blood transfusion services to improve storage and transportation conditions of the blood bags. © 2014 European Federation for Medical Informatics and IOS Press.

Habibi E.,Mazandaran University of Medical Sciences | Shokrzadeh M.,Mazandaran University of Medical Sciences | Chabra A.,Stud Research Committee | Naghshvar F.,Mazandaran University of Medical Sciences | Ahmadi A.,Mazandaran University of Medical Sciences
Pharmaceutical Biology | Year: 2015

Context: Despite its wide clinical use, cyclophosphamide (CP), an alkylating chemotherapeutic agent, possesses many adverse effects, including hepatotoxicity. Because Origanum vulgare L. (Lamiaceae) has antioxidative properties, it might protect against above-mentioned damage. Objective: This study evaluated the protective effects of O. vulgare extract on CP-induced liver toxicity. Materials and methods: Mice were pretreated with aerial parts of O. vulgare ethanolic extract (intraperitoneally) at doses of 50, 100, 200, and 400 mg/kg for 7 consecutive days before the administration of a single 200 mg/kg intraperitoneal dose of CP 1 h after the last injection of O. vulgare. After 24 h, animals were anesthetized, blood samples and hepatic tissues were collected and used for biochemical and histological examination. Results: Serum levels of hepatic markers were increased after CP treatment but restored in the O. vulgare-pretreated groups. The serum ALT, AST, and ALP of the CP group were 196.49 ± 3.82, 143.78 ± 4.79, and 203.18 ± 3.81 IU/l, respectively. However, pretreatment with 400 mg/kg O. vulgare significantly decreased the serum ALT, AST, and ALP to 52.49 ± 2.18, 44.78 ± 2.06, and 65.62 ± 1.73 IU/l, respectively (p < 0.001). Histological examinations also confirmed the protective effects of O. vulgare against CP-induced liver toxicity. Discussion and conclusion: Our results reveal that O. vulgare with high amount of flavonoids and phenolic compounds induces potent hepatoprotective mechanisms against CP. Therefore, O. vulgare might help defend the body against the side effects, particularly hepatic damages induced by chemotherapeutic agents. © 2015 Informa Healthcare USA, Inc. All rights reserved.

Habibi E.,Mazandaran University of Medical Sciences | Shokrzadeh M.,Mazandaran University of Medical Sciences | Ahmadi A.,Mazandaran University of Medical Sciences | Chabra A.,Stud Research Committee | Naghshvar F.,Mazandaran University of Medical Sciences
Pharmaceutical Biology | Year: 2015

Context: Cyclophosphamide (CP), an alkylating chemotherapeutic agent, can bind DNA, causing chromosome breaks, micronucleus (Mn) formation, and cell death. Because Origanum vulgare L. (Lamiaceae) has antioxidative properties, it might protect against DNA damage. Objective: The genoprotective effect of O. vulgare ethanolic extract against CP-induced genotoxicity in mouse bone marrow cells was evaluated using a Mn assay. Materials and methods: Mice were pre-treated with aerial parts of O. vulgare ethanolic extract at different doses (50, 100, 200, or 400 mg/kg) for 7 d. One hour after the last administration of O. vulgare, animals were injected with CP at 200 mg/kg. After 24 h, the bone marrow cells of both femurs were flushed and the frequency of MnPCEs was evaluated to measure the chromosomal damages. In addition, the number of PCEs per 1000 NCEs in each animal was recorded to evaluate the bone-marrow suppression; mitotic activity was calculated as [PCE/(PCE + NCE)] × 100 to assess the cell division. Results: At 400 mg/kg, O. vulgare displayed its maximum protective effect, reduced the number of MnPCEs from 10.52 ± 1.07 for CP group to 2.17 ± 0.26 and completely normalized the mitotic activity (p < 0.001). Origanum vulgare also led to significant proliferation and hypercellularity of immature myeloid elements after the mice were treated with CP, mitigating the bone marrow suppression. Discussion and conclusion: Origanum vulgare ethanolic extract exerts a potent genoprotective effect against CP-induced genotoxicity in mice bone marrow, which might be possibly due to the scavenging of free radicals during oxidative stress conditions. © 2015 Informa Healthcare USA, Inc. All rights reserved.

Norozi B.,Stud Research Committee | Atashi S.,Kermanshah University of Medical Sciences | Farahani A.,Stud Research Committee
Journal of Global Infectious Diseases | Year: 2014

Background and Objective: Mycobacterium tuberculosis has developed resistance to antituberculosis drugs and becoming a major and alarming public health problem in worldwide. This study was aimed to determine antituberculosis drug resistance rate and to identify multidrug resistant tuberculosis (MDR-TB) in West of Iran. Materials and Methods: Of 130 samples were included between December 2011 and July 2012 in the study from that 112 cases were M. tuberculosis. The proportional method was carried out according to the Clinical and Laboratory Standards Institute on Lowenstein-Jensen against isoniazid, rifampicin, streptomycin, ethambutol, pyrazinamide, para aminosalicylic acid, ethionamide, cycloserine (CYC). The microdilution method was carried out using 7H9 broth with 96 well-plates. Results: From 112 isolates, resistance was observed to isoniazid 18 (16.07%), rifampicin 16 (14.28%), streptomycin 25 (22.32%), ethambutol 15 (13.39%), pyrazinamide 27 (24.10%), para aminosalicylic acid 19 (16.96%), CYC 4 (3.57%), and ethionamide 14 (12.5%) cases. 16 isolates were MDR. Conclusion: The high prevalence of MDR-TB in our study is assumed to be due to recent transmission of drug-resistant strains. Overall, the rate of drug resistance in our study was high, which is in line with findings of some high-burden countries. Hence that early case detection, rapid drug susceptibility testing, and effective anti-TB treatment is necessary. © 2014 Journal of Global Infectious Diseases.

Janghorban R.,Stud Research Committee | Roudsari R.L.,Evidence Based Care Research Center | Taghipour A.,Mashhad University of Medical Sciences
International Journal of Qualitative Studies on Health and Well-being | Year: 2014

The most commonly used method for data collection in qualitative research is interviewing. With technology changes over the last few decades, the online interview has overcome time and financial constraints, geographical dispersion, and physical mobility boundaries, which have adversely affected onsite interviews. Skype as a synchronous online service offers researchers the possibility of conducting individual interviews as well as small focus groups, comparable to onsite types. This commentary presents the characteristics of the Skype interview as an alternative or supplemental choice to investigators who want to change their conventional approach of interviewing. © 2014 R. Janghorban et al.

Mohajeri P.,Kermanshah University of Medical Sciences | Sharbati S.,Stud Research Committee | Farahani A.,Kermanshah University of Medical Sciences | Rezaei Z.,Stud Research Committee
Journal of Natural Science, Biology and Medicine | Year: 2016

Background: Acinetobacter baumannii which is a Gram-negative bacterium can cause several different infections. The appearance of carbapenemase-producing A. baumannii in recent years has made the treatment process more difficult. The identification of virulence factors (VFs), such as nonadhesives in A. baumannii, helps to fight against related infections. Materials and Methods: A total of 104 samples from teaching hospitals in Kermanshah, Iran, were collected during a 24 months period (2011-2013). Sample identification was first carried out by biochemical tests, and then their susceptibility to carbapenems was determined using the Kirby-Bauer method. For confirmation of carbapenemase-producing A. baumannii, polymerase chain reaction (PCR) was done for carbapenemase-encoding genes. In addition, the frequency of nonadhesive VFs in carbapenemase-producing isolates was determined by PCR. Results: There were 50 isolates that were identified as carbapenemase-producing A. baumannii. The PCR results showed; 40 isolates (80%) for traT, 17 isolates (34%) for cvaC, and 8 isolates (16%) for iutA, and these encode serum resistance, colicin V and aerobactin, respectively. No significant correlation was observed between these three genes. Conclusions: The mechanism of A. baumannii virulence has always been in question. The role of VFs has also been recognized in other Gram-negative bacteria. According to the prevalence of traT, cvaC and iutA, as nonadhesive VFs, we can suggest that they could be the main mechanism of carbapenemase-producing A. baumannii pathogenesis. © 2016 Journal of Natural Science, Biology and Medicine | Published by Wolters Kluwer - Medknow.

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