Stomatological Hospital of Jiangsu Province

Nanjing, China

Stomatological Hospital of Jiangsu Province

Nanjing, China

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Wang Z.L.,Nanjing Medical University | Tang Z.C.,Nanjing Medical University | Tang Z.C.,Tongling Peoples Hospital | Zhang Y.,First Peoples Hospital of Xuzhou | And 12 more authors.
Head and Neck Oncology | Year: 2012

Objective Our previous study demonstrated that neuropilin-1 is useful for the prognosis of tongue squamous cell carcinoma. In this study, we aimed to explore the role of neuropilin-1 in the differentiation of tongue squamous cell carcinoma. Methods Forty primary tongue squamous cell carcinoma samples were subjected to immunostaining for neuropilin-1. The relationship between neuropilin- 1 expression and differentiation was analysed. A specific small hairpin ribonucleic acid (shRNA) targeting neuropilin-1 was transfected into the tongue squamous cell carcinoma cell line squamous cell carcinoma-25 to knockdown neuropilin-1 expression. Scratch and transwell assays were performed to examine the cell migration and invasion ability after neuropilin- 1 was knockeddown. Real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to detect the efficiency of neuropilin-1 knockdown and the expression of differentiation markers. Results We found a significant correlation between the high neuropilin-1 expression level and the poor histopathological differentiation of tongue squamous cell carcinoma (p = 0.026). Neuropilin-1 was expressed at a high level in squamous cell carcinoma-25 cells. After neuropilin-1 knockdown via shRNA, cell migration and invasion were significantly reduced as demonstrated by scratch and transwell assays (p < 0.01). In addition, Western blotting and RT-PCR demonstrated that neuropilin-1 knockdown increased E-cadherin and decreased fibronectin and vimentin expression in squamous cell carcinoma-25 cells. Conclusion Neuropilin-1 knockdown may inhibit the migration and invasion of tongue squamous cell carcinoma by inducing a more differentiated cancer state. Considering the high level of neuropilin- 1 expression in tongue squamous cell carcinoma, it may serve as a useful marker for differentiation and as a target for the treatment of tongue cancer. Copyright © 2012 OA Publishing London.


Zhu J.,Nanjing Medical University | Zhu J.,Stomatological Hospital of Jiangsu Province | Wu Y.-N.,Stomatological Hospital of Jiangsu Province | Zhang W.,Stomatological Hospital of Jiangsu Province | And 7 more authors.
PLoS ONE | Year: 2014

Monocarboxylate transporter 4 (MCT4) is a cell membrane transporter of lactate. Recent studies have shown that MCT4 is over-expressed in various cancers; however, its role in cancer maintenance and aggressiveness has not been fully demonstrated. This study investigated the role of MCT4 in oral squamous cell carcinoma (OSCC), and found that it is highly expressed in OSCC patients by using immunohistochemistry. Moreover, this over-expression of MCT4 was closely associated with tumor size, TNM classification, lymphatic metastasis, distant metastasis and tumor recurrence, and also poor prognosis. To further study mechanisms of MCT4 in vitro, we used small-interfering RNA to silence its expression in OSCC cell lines. The results showed that knock-down of MCT4 decreased cell proliferation, migration, and invasion. The inhibition of proliferation was associated with down-regulation of p-AKT and p-ERK1/2, while decreased cell migration and invasion may be caused by down-regulation of integrin β4-SRC-FAK and MEK-ERK signaling. Together, these findings provide new insight into the critical role of MCT4 in cell proliferation and metastasis in OSCC. © 2014 Zhu et al.


PubMed | Nanjing Medical University, Stomatological Hospital of Jiangsu Province and CAS Shanghai Institute of Materia Medica
Type: Journal Article | Journal: PloS one | Year: 2014

Monocarboxylate transporter 4 (MCT4) is a cell membrane transporter of lactate. Recent studies have shown that MCT4 is over-expressed in various cancers; however, its role in cancer maintenance and aggressiveness has not been fully demonstrated. This study investigated the role of MCT4 in oral squamous cell carcinoma (OSCC), and found that it is highly expressed in OSCC patients by using immunohistochemistry. Moreover, this over-expression of MCT4 was closely associated with tumor size, TNM classification, lymphatic metastasis, distant metastasis and tumor recurrence, and also poor prognosis. To further study mechanisms of MCT4 in vitro, we used small-interfering RNA to silence its expression in OSCC cell lines. The results showed that knock-down of MCT4 decreased cell proliferation, migration, and invasion. The inhibition of proliferation was associated with down-regulation of p-AKT and p-ERK1/2, while decreased cell migration and invasion may be caused by down-regulation of integrin 4-SRC-FAK and MEK-ERK signaling. Together, these findings provide new insight into the critical role of MCT4 in cell proliferation and metastasis in OSCC.


Pu L.-F.,Nanjing Medical University | Pu L.-F.,Stomatological Hospital of Jiangsu Province | Pu L.-F.,Nanjing Maternity and Child Health Care Hospital | Tang C.-B.,Nanjing Medical University | And 13 more authors.
International Journal of Oral and Maxillofacial Surgery | Year: 2014

The objectives of this study were to verify whether Chinese patients are well-suited for zygomatic implantation and to observe age-related changes in the linear and angular anatomic bases of the maxilla and zygoma. Using three-dimensional images selected from maxillofacial cone beam computed tomography (CBCT) scans generated by SimPlant, linear and angular measurements were obtained by simulating zygomatic implantation. The edentulous group comprised 40 subjects aged between 62 and 65 years. A total of 120 dentate cases were divided into three groups based on age: the established occlusion group (n = 40; 12-15 years old), the adult group (n = 40; 37-40 years old), and the elderly group (n = 40; 62-65 years old). The mean potential insertion length of the ordinary and additional zygomatic implants became longer with age in the dentate groups. For both zygomatic implant insertion areas, the anteroposterior lengths of the maxilla and zygoma were thicker in the older dentate groups (P < 0.05). Significant differences were verified in the installation direction among the dentate groups. Gender was not a significant factor. The zygomatic skeleton changes with age, which results in linear and angular variations in the zygomatic implant insertion area. Therefore, the anatomic bases in Chinese adults are suitable for zygomatic implants. © 2014 International Association of Oral and Maxillofacial Surgeons.


Zhang Y.,Nanjing Medical University | Zhang Y.,Stomatological Hospital of Jiangsu Province | Wang R.,Nanjing Medical University | Miao L.,Nanjing Medical University | And 5 more authors.
PLoS ONE | Year: 2015

Objective To provide a precise quantification of the association between alcohol and tobacco consumption trends in head and neck cancer patients over the past 45 years. Methods We combined findings from all studies published until March 2014 and evaluated the association between different levels in alcohol and tobacco consumption and head and neck cancers through a meta-analytic approach. Results We included 28 studies involving 13830 patients with head and neck cancer. In patients with alcohol consumption, the pooled odds ratio (OR) and 95% confidence interval (CI) were 1.29(1.06-1.57), 2.67(2.05-3.48) and 6.63(5.02-8.74) for light drinkers, moderate drinkers and heavy drinkers, respectively. In patients with tobacco consumption, the pooled OR and 95% CI were 2.33(1.84-2.95), 4.97(3.67-6.71) and 6.77(4.81-9.53) for light smokers, moderate smokers and heavy smokers, respectively. Conclusion The increased alcohol and tobacco consumption trends increased the risk of head and neck cancer over the past 45 years. Tobacco consumption was found to be a stronger risk factor for head and neck cancer than alcohol consumption. Thus, the control should be considered to limit the intake of alcohol and tobacco. Copyright: © 2015 Zhang et al.


Liu L.,Shanghai Stomatological Diseases Center | Liu S.,Shanghai Pudong Zhoujiadu Community Health Center | Song X.,Nanjing Medical University | Song X.,Stomatological Hospital of Jiangsu Province | And 4 more authors.
Lasers in Medical Science | Year: 2013

This study investigated the effect of neodymium-doped yttrium aluminum garnet (Nd: YAG) laser irradiation on surface properties and bond strength of zirconia ceramics. Specimens of zirconia ceramic pieces were divided into 11 groups according to surface treatments as follows: one control group (no treatment), one air abrasion group, and nine laser groups (Nd: YAG irradiation). The laser groups were divided by applying with different output power (1, 2, or 3 W) and irradiation time (30, 60, or 90 s). Following surface treatments, the morphological characteristics of ceramic pieces was observed, and the surface roughness was measured. All specimens were bonded to resin cement. After, stored in water for 24 h and additionally aged by thermocycling, the shear bond strength was measured. Dunnett's t test and one-way ANOVA were performed as the statistical analyses for the surface roughness and the shear bond strength, respectively, with α = .05. Rougher surface of the ceramics could be obtained by laser irradiation with higher output power (2 and 3 W). However, cracks and defects were also found on material surface. The shear bond strength of laser groups was not obviously increased, and it was significantly lower than that of air abrasion group. No significant differences of the shear bond strength were found among laser groups treated with different output power or irradiation time. Nd: YAG laser irradiation cannot improve the surface properties of zirconia ceramics and cannot increase the bond strength of the ceramics. Enhancing irradiation power and extending irradiation time cannot induce higher bond strength of the ceramics and may cause material defect. © 2013 Springer-Verlag London.


Chu W.,Nanjing Medical University | Chu W.,Stomatological Hospital of Jiangsu Province | Song X.,Nanjing Medical University | Song X.,Stomatological Hospital of Jiangsu Province | And 8 more authors.
PLoS ONE | Year: 2014

Background: The epithelial-to-mesenchymal transition (EMT) is a key process in carcinogenesis, invasion, and metastasis of oral squamous cell carcinoma (OSCC). In our previous studies, we found that neuropilin-1 (NRP1) is overexpressed in tongue squamous cell carcinoma and that this overexpression is associated with cell migration and invasion. Nuclear factor-kappa B (NF-κB) plays an essential role both in the induction and the maintenance of EMT and tumor metastasis. Therefore, we hypothesized that NRP1 induces EMT, and that NRP1-induced migration and invasion may be an important mechanism for promoting invasion and metastasis of OSCC through NF-κB activation. Methods/Results: The variations in gene and protein expression and the changes in the biological behavior of OSCC cell lines transfected with a vector encoding NRP1, or the corresponding vector control, were evaluated. NRP1 overexpression promoted EMT and was associated with enhanced invasive and metastatic properties. Furthermore, the induction of EMT promoted the acquisition of some cancer stem cell (CSC)-like characteristics in OSCC cells. We addressed whether selective inhibition of NF-κB suppresses the NRP1-mediated EMT by treating cells with pyrrolidinedithiocarbamate ammonium (PDTC), an inhibitor of NF-κB. Immunohistochemical analysis of NRP1 in OSCC tissue samples further supported a key mediator role for NRP1 in tumor progression, lymph node metastasis, and indicated that NRP1 is a predictor for poor prognosis in OSCC patients. Conclusion: Our results indicate that NRP1 may regulate the EMT process in OSCC cell lines through NF-κB activation, and that higher NRP1 expression levels are associated with lymph node metastasis and poor prognosis in OSCC patients. Further investigation of the role of NRP1 in tumorigenesis may help identify novel targets for the prevention and therapy of oral cancers. © 2014 Chu et al.


Chu W.M.,Nanjing Medical University | Chu W.M.,Stomatological Hospital of Jiangsu Province | Ma L.,Nanjing Medical University | Ma L.,Stomatological Hospital of Jiangsu Province | And 5 more authors.
Head and Neck Oncology | Year: 2013

Backgrounds: Matrix metalloproteinase-1 (MMP-1), is the most abundant member of the MMPs family. The association between MMP-1 (-1607) 1G/2G polymorphism and head/neck cancer development and progression has been analyzed in several studies. However, the published results are inconsistent. To elucidate the association, we performed this meta-analysis. Methods: All studies evaluating the association between MMP-1 polymorphism and head/neck cancer were identified by a search in PubMed database. Odds ratios (ORs) with 95% confidence interval (CIs) were used to evaluate the strength of the association. Subgroup analyses by different ethnicity and clinicopathological characteristics were performed to further identify the association. Statistical analysis was performed with Review Manager 5.0. Results: Twelve individual case-control studies with a total of 4, 599 subjects (2, 115 cases and 2, 484 controls) were included into this meta-analysis. An overall OR of 1.33 (95% CI=1.10-1.62, P=0.004) was found for 2G allele, with significant results observed under dominant (OR=1.33, 95% CI=1.01-1.77, P=0.05) and recessive genetic model (OR=1.55, 95% CI=1.15-2.08, P=0.004). Consistently, a increased risk was calculated in the subgroup of Asian population. In the stratified analysis according to pathological staging, we found increased risk of advanced stages (III-IV) in 2G genotype carriers. Conclusions: This meta-analysis demonstrates that MMP-1 (-1607) 1G/2G polymorphism is significantly associated with increased risk of head/neck cancer development, and the race-specific effect may exist in this association. In addition, the polymorphism may lead to advanced cancer stages (III-IV).


PubMed | Nanjing Medical University and Stomatological Hospital of Jiangsu Province
Type: Comparative Study | Journal: PloS one | Year: 2014

The epithelial-to-mesenchymal transition (EMT) is a key process in carcinogenesis, invasion, and metastasis of oral squamous cell carcinoma (OSCC). In our previous studies, we found that neuropilin-1 (NRP1) is overexpressed in tongue squamous cell carcinoma and that this overexpression is associated with cell migration and invasion. Nuclear factor-kappa B (NF-B) plays an essential role both in the induction and the maintenance of EMT and tumor metastasis. Therefore, we hypothesized that NRP1 induces EMT, and that NRP1-induced migration and invasion may be an important mechanism for promoting invasion and metastasis of OSCC through NF-B activation.The variations in gene and protein expression and the changes in the biological behavior of OSCC cell lines transfected with a vector encoding NRP1, or the corresponding vector control, were evaluated. NRP1 overexpression promoted EMT and was associated with enhanced invasive and metastatic properties. Furthermore, the induction of EMT promoted the acquisition of some cancer stem cell (CSC)-like characteristics in OSCC cells. We addressed whether selective inhibition of NF-B suppresses the NRP1-mediated EMT by treating cells with pyrrolidinedithiocarbamate ammonium (PDTC), an inhibitor of NF-B. Immunohistochemical analysis of NRP1 in OSCC tissue samples further supported a key mediator role for NRP1 in tumor progression, lymph node metastasis, and indicated that NRP1 is a predictor for poor prognosis in OSCC patients.Our results indicate that NRP1 may regulate the EMT process in OSCC cell lines through NF-B activation, and that higher NRP1 expression levels are associated with lymph node metastasis and poor prognosis in OSCC patients. Further investigation of the role of NRP1 in tumorigenesis may help identify novel targets for the prevention and therapy of oral cancers.

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