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Ruan G.-P.,Stem Cell Engineering Laboratory | Yao X.,Stem Cell Engineering Laboratory | Liu J.-F.,Stem Cell Engineering Laboratory | Shu F.,Stem Cell Engineering Laboratory | And 4 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Umbilical cord as childbirth waste has wide variety of sources and can be easily obtained, without any ethical and legal restrictions. Therefore, human umbilical cord mesenchymal stem cells break all the restrictions originated from other sources of mesenchymal stem cells. OBJECTIVE: To review the application of human umbilical cord mesenchymal stem cells in cartilage diseases, neuroglioma, ischemic brain injury, lung disease, liver disease and models of myocardial infarction. METHODS: The PubMed and Wanfang databases were searched by the first author using the keywords of “human umbilical cord mesenchymal stem cells, disease models, cell therapy” in English and Chinese, respectively. Seventy-three articles were searched and finally, 35 articles were included in result analysis. RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells have multilineage differentiation capacity similar to bone marrow mesenchymal stem cells. Compared with bone marrow mesenchymal stem cells, human umbilical cord mesenchymal stem cells have lower immunogenicity. Human umbilical cord mesenchymal stem cells show certain curative effects on cartilage disease, neuroglioma, ischemic brain injury, lung disease, liver disease and myocardial infarction, indicating that human umbilical cord mesenchymal stem cells can be used for cell transplantation to treat various diseases. © 2015 Journal of Clinical Rehabilitative Tissue Engineering Research, All rights reserved. Source


Ruan G.-P.,Stem Cell Engineering Laboratory | Wang J.-X.,Stem Cell Engineering Laboratory | Yang J.-Y.,Stem Cell Engineering Laboratory | Liu J.-F.,Stem Cell Engineering Laboratory | And 4 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: There are less studies addressing whether bone marrow mesenchymal stem cells from systemic lupus erythematosus patients are different from healthy people. Objective: To compare the multi-differentiation capacity of bone marrow mesenchymal stem cells isolated from systemic lupus erythematosus model mice and normal control mice. Methods: Bone marrow mesenchymal stem cells of systemic lupus erythematosus model mice and C57BL mice were isolated and cultured followed by osteogenic and adipogenic differentiations, respectively. Differentiation abilities of two kinds of mouse bone marrow mesenchymal stem cells were observed. Results and Conclusion: Passaged bone marrow mesenchymal stem cells from C57BL mice were long spindle-shaped and evenly distributed, while bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice showed slow growth and were relatively smaller than those from C57BL mice. After osteogenic induction, the amount of calcium salt and calcium nodules were significantly less in the bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice than from normal control mice. PCR detection showed that expressions of Runx2, alkaline phosphatase and osteocalcin were also significantly decreased in the bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice. After adipogenic induction, the number of lipid droplets in the bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice was significantly less than the control group, and PCR detection also showed significantly decreased expression of adipogenic genes, including PPARγ2 and lipoprotein lipase. These findings suggest that the bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice exhibit lower osteogenic and adipogenic capacities than those from normal C57BL mice, and also have the impaired multi-differentiation ability. Source


Ruan G.-P.,Stem Cell Engineering Laboratory | Yao X.,Stem Cell Engineering Laboratory | Liu J.-F.,Stem Cell Engineering Laboratory | Shu F.,Stem Cell Engineering Laboratory | And 6 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Reprogramming somatic cells to generate pluripotent stem cells has a wide application in biomedical research. OBJECTIVE: To analyze the effect of different molecular weight proteins in chicken egg-white extract to elevate expression of pluripotent genes Oct-3/4 and Nanog in 293T cells. METHODS: The extracts of chicken egg-white were separated into more than 3 ku and less than 3 ku ingredients to be used for co-culture with 293T cells. There were four groups, 1×105 293T cells per well, total 500 μL. In the control group, 500 μL culture medium was added; in the other three groups, 500 μL chicken egg-white extract, more than 3 ku and less than 3 ku ingredients were respectively added. Quantitative PCR was used to determine the relative expression levels of pluripotent genes Nanog and Oct-3/4 in 293T cells. RESULTS AND CONCLUSION: By using co-culture method, more than 3 ku ingredients have a role to increase the expression of pluripotent genes Oct-3/4 and Nanog, but less than 3 ku ingredients cannot elevate the expression of pluripotent genes. This indicates that the ingredient of chicken egg-white extract to elevate the expression of pluripotent genes is more than 3 ku proteins. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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