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Puiu M.,Victor Babes University of Medicine and Pharmacy Timisoara | Rusu C.,Grigore T. Popa University of Medicine and Pharmacy | Badiu C.,CI Parhon Endocrynology Institute | Dan D.,Romanian Prader Willi Association | And 2 more authors.
Revista Romana de Medicina de Laborator | Year: 2010

Prader Willi Syndrome (PWS) is a neurometabolic genetic disorder affecting 1/12.000-1/15.000 new-borns. Molecular mechanisms that could lead to this disorder include chromosomal deletions, uniparental disomy (UPD), intragenic mutations, and epigenetic modifications in the process of imprinting and rarely reciprocal translocations. A common defect is noticed in all cases: loss of parental contribution for the functioning of specific genes in normal conditions, due to genetic instability of the critical region 15q11-q13. Objectives of the study concerned the implementation of molecular genetic/ epigenetic methods of investigation and development of an interdisciplinary clinical investigation algorithm specific for the disease (geneticist, pediatrician, endocrinologist, psychiatrist, neurologist, psychologist, orthopedist, pneumologist, nutritionist) aiming for early re-cognition of the clinical features, resulting in early diagnosis and early intervention. Materials and methods: a multicentric study started in 2008, being included in a research project (CNMP/Partnerships, 2008-2011), and coordinated by UMF Timisoara. So far, 9 suspected cases of PWS have been investigated and in 3 cases family members have been tested (brother, sister, parents, grandparents). Results: The investigation protocol applied, including multidisciplinary clinical evaluation, laboratory investigations, and specific genetic and epigenetic tests relevant for the subtypes of PWS, allowed accurate diagnosis of patients. This approach was applied for the first time in our country. Conclusions: Early recognition and diagnosis is essential in PWS, as complex treatment applied in due time leads to prevention of obesity installation and other redoubtable complications. This is possible by interdisciplinary approach and detection of molecular mechanisms involved, allowing an appropriate genetic counseling.


Grumezescu A.M.,Polytechnic University of Bucharest | Andronescu E.,Polytechnic University of Bucharest | Albu M.G.,INCDTP Leather and Footwear Res Institute | Ficai A.,Polytechnic University of Bucharest | And 4 more authors.
Current Organic Chemistry | Year: 2013

The aims of this research were to obtain improved wound dressings based on collagen (COLL), polysaccharides (dextran= DEX, diethylaminoethyl-cellulose= DEAEC), silica network and usnic acid, as quorum sensing inhibitor. FT-IR, SEM, interaction with eukaryotic cells and a novel protocol to evaluate the antimicrobial activity of the new wound dressing, firstly reported in literature were used for the characterization of fabricated wound dressings. The obtained wound dressings are not cytotoxic, do not influences the mes-enchymal stem and exhibit good anti-biofilm properties. Taken together, these results are suggesting that the new systems can be safely used for local applications on the lesional tissues. © 2013 Bentham Science Publishers.


Anghel I.,Carol Davila University of Medicine and Pharmacy | Limban C.,Carol Davila University of Medicine and Pharmacy | Grumezescu A.M.,Polytechnic University of Bucharest | Anghel A.G.,Carol Davila University of Medicine and Pharmacy | And 2 more authors.
Nanoscale Research Letters | Year: 2012

In this paper, we report the design of a new nanofluid for anti-pathogenic surface coating. For this purpose, new 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides were synthesized and used as an adsorption shell for Fe3O4/C12 core/shell nanosized material. The functionalized specimens were tested by in vitro assays for their anti-biofilm properties and biocompatibility. The optimized catheter sections showed an improved resistance to Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 in vitro biofilm development, as demonstrated by the viable cell counts of biofilm-embedded bacterial cells and by scanning electron microscopy examination of the colonized surfaces. The nanofluid proved to be not cytotoxic and did not influence the eukaryotic cell cycle. These results could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with improved anti-biofilm properties. © 2012 Anghel et al.


Galatescu A.,National Institute for R and D in Informatics | Alexandru A.,National Institute for R and D in Informatics | Zaharia C.,Stefan Nicolau Institute of Virology | Popovici A.,Stefan Nicolau Institute of Virology
Studies in Informatics and Control | Year: 2011

The paper presents the conceptual framework, the basic inference and some implementation results in a system (under development) for the online training on the potential risks and events that can occur during the execution of certain activities. This system is an application of ontologies in the occupational health domain. The training (in a requested context and complying with domain-specific rules) results from the automatic and semantics-based discovery of the prevention documents, actions, methods etc that fit the user's request.

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