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Pavela R.,Czech Republic Crop Research Institute | Zabka M.,Czech Republic Crop Research Institute | Kalinkin V.,The branch office of All Russian Institute of Plant Protection VIZR | Kotenev E.,Stavropol Research Antiplague Institute | And 3 more authors.
Plant Protection Science | Year: 2013

In 2010 and 2011, the efficiency of azadirachtin, applied as systemic trunk injections in the trunks of Platanus sp., was tested against Corythucha ciliata. Azadirachtin in the doses of 0.1 and 0.05 g of active ingredient per cm of diameter at breast height was applied in April. It was found that after application of both doses, the count of C. ciliata in plane-tree leaves significantly decreased. In 2010 and 2011, the average counted number of C. ciliata individuals in trees treated with the dose 0.1 g a.i./cm of dbh was 12.9 and 4.9, respectively, and 29.1 and 6.5 individuals, respectively, in trees treated with the dose 0.05 g a.i./cm of dbh, in the control it was 152.3 and 105.8 individuals, respectively. © 2011 Czech Academy of Agricultural Sciences. Source


Platonov M.E.,State Research Center for Applied Microbiology and Biotechnology | Evseeva V.V.,State Research Center for Applied Microbiology and Biotechnology | Svetoch T.E.,State Research Center for Applied Microbiology and Biotechnology | Efremenko D.V.,Stavropol Research Antiplague Institute | And 4 more authors.
Molecular Genetics, Microbiology and Virology | Year: 2012

Comparative analysis of 57 strains of Y. pestis subsp. microtus bv. caucasica was carried out using molecular typing. The results obtained indicate the presence of three independent phylogenetic groups and indicate the advisability of isolation of the Leninakan mountain mesofocus from the Transcaucasian highland focus into an independent focus, as well as inclusion of part of the Pre-Araks low-mountain focus as the mesofocus along with the Pre-Sevan mountain and Zangezur-Karabakh mountain mesofoci into the Tran- scaucasian highland plague focus. It is shown that the strains circulating in the East Caucasus highland focus of plague are the most ancient branch of the caucasica biovar, and possibly of the entire phylogenetic tree of Y. pestis. © Allerton Press, Inc., 2012. Source


Evseeva V.V.,State Scientific Center for Applied Microbiology and Biotechnology | Platonov M.E.,State Scientific Center for Applied Microbiology and Biotechnology | Govorunov I.G.,State Scientific Center for Applied Microbiology and Biotechnology | Efremenko D.V.,Stavropol Research Antiplague Institute | And 4 more authors.
Molecular Genetics, Microbiology and Virology | Year: 2016

Comparative analysis of MLVA25-typing and MLVA7-typing for their ability to identify focal affiliation of the Yersinia pestis strains have been performed on the basis of the bv. Medievalis isolates obtained from the Central Caucasus highland natural plague focus. For purposes of analysis, MLVA25 genotypes were identified for 82 isolates and further introduced into a database containing data on the 949 strains of the plague-causing agent obtained in other natural foci in Russia and countries within the former Soviet Union and beyond. The dendrograms were reconstructed by the UPGMA method with a categorical coefficient using data on all 25 VNTR loci or on only 7 of them, which were recommended by the experts from the Microbe Russian Research Anti-Plague Institute to be used for Y. pestis strains differentiation by their focal affiliation. The results of the analysis indicate the higher possibility of diagnostic errors when using the MLVA7-typing for distinguishing between the Y. pestis strains and suggest that it should be useful to subdivide the Central Caucasus highland plague focus into two subfoci. © 2016, Allerton Press, Inc. Source


Platonov M.E.,State Research Center for Applied Microbiology and Biotechnology | Evseeva V.V.,State Research Center for Applied Microbiology and Biotechnology | Efremenko D.V.,Stavropol Research Antiplague Institute | Afanas'ev M.V.,Antiplague Research Institute for Siberia and the Far East | And 7 more authors.
Molecular Genetics, Microbiology and Virology | Year: 2015

Comparative analysis of rhamnose-positive Y. pestis strains, which are traditionally recognized as the biovar altaica, was performed with the use of molecular typing. The strains were isolated from the natural plague foci of the Changay mountainous system and the Sailugem natural plague focus in Mongolia. The Russian part of the latter is known as the Altai Mountains focus (focus 36). All isolates were compared with the other rhamnose-positive vole strains from the natural plague foci located in the grassland of Xilingol (focus L, China), the plateau of Qinghai-Tibet (focus M, China), the Gissarian Ridge (focus 34, Tadjikistan and Uzbekistan), and the Talassian Ridge (focus 40, Kyrgyzstan). The strains studied formed a single cluster with two branches, of which the first branch included the talassica (0.PE4?), qinghaiensis (0.PE4ab), and xilingolensis (0.PE4cd) phylogenetic groups, while the second included the hissarica (0.PE9) and altaica (0.PE1) phylogenetic groups. The results of molecular typing suggest that several strains from the Qinghai group of the Mongolian foci belong to the qinghaiensis and xilingolensis biovars but not to the altaica biovar. © 2015, Allerton Press, Inc. Source


Kulichenko A.N.,Stavropol Research Antiplague Institute | Mikhailova M.E.,Stavropol Research Antiplague Institute | Kovalev D.A.,Stavropol Research Antiplague Institute | Pisarenko S.V.,Stavropol Research Antiplague Institute | And 2 more authors.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk | Year: 2014

Aim: to study features of pharmacokinetics of ofloxacin as a part of anion PEGylated niosomes on a basis of sorbitan monostearate (Span 60) to experimental white mice per os. Materials and methods: ofloxacin was entrapped in niosomes consisting of Span 60, cholesterol, PEG 4000 and dicetylphosphate. Sizes of niosomes estimated by means of probe microscopy. Efficiency of inclusion of an antibiotic in niosomes defined after removal of free drug by a centrifugation. The analysis of the quantitative contents of ofloxacin in samples carried out a method of a high performance liquid chromatography. Results: we studied the main pharmacokinetic parameters of ofloxacin when used free and niosomal forms of antibiotic to experimental white mice per os. It is shown that use of oral niosomal forms leads to decrease of maximal concentration in serum and increase of ofloxacin half-life by 7,4 times in average compared to the free form. It is determined that bioavailability of ofloxacin in the niosomal form is 154% relative to the free form of the antibiotic. Conclusions: niosomal microcontainers are perspective technology of encapsulation and the directed transport of antibacterial preparations through biological barriers. Using of niosomal formulation of ofloxacin is able to afford to increase considerably efficiency of treatment in comparison with a free form and significantly decrease negative effects of antibiotic therapy. Source

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