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Volynkina A.S.,Stavropol Research Anti Plague Institute | Vasilenko N.F.,Stavropol Research Anti Plague Institute
Molecular Genetics, Microbiology and Virology | Year: 2014

A genetic analysis of the Crimean-Congo hemorrhagic fever (CCHF) virus circulating in Stavropol krai in 2011 was performed. A total of 14 RNA isolates from patients suffering from Crimean hemorrhagic fever were genetically typed. Genetic analysis of the CCHF virus strains based on M-segment sequences (positions 2607–2932) supports the notion that the genotype Europe 1 is circulating in Stavropol krai of Russia. In addition to the previously known lineage STV-ROS, the second lineage VLG/ROS was observed in Stavropol krai. © 2014, Allerton Press, Inc.


PubMed | Stavropol Research Anti Plague Institute
Type: | Journal: Advances in experimental medicine and biology | Year: 2016

This chapter summarizes information about the natural foci of plague in the world. We describe the location, main hosts, and vectors of Yersinia pestis. The ecological features of the hosts and vectors of plague are listed, including predators - birds and mammals and their role in the epizootic. The epizootic process in plague and the factors affecting the dynamics of epizootic activity of natural foci of Y. pestis are described in detail. The mathematical models of the epizootic process in plague and predictive models are briefly described. The most comprehensive list of the hosts and vectors of Y. pestis in the world is presented as well.


Trukhachev V.,Stavropol State Agrarian University | Skripkin V.,Stavropol State Agrarian University | Kvochko A.,Stavropol State Agrarian University | Kulichenko A.,Stavropol Research Anti plague Institute | And 6 more authors.
Revista Colombiana de Ciencias Pecuarias | Year: 2016

Background: marker assisted selection methods of sheep require the identification of genes that positively and negatively affect meat quality. Genes with high expression levels could have the greatest impact on growth and structure of muscle fibers. Objective: this study evaluated the expression of genes in the loin muscle of Dzhalginsky Merino sheep. Methods: reverse transcription-quantitative real-time PCR (RT-qPCR) was used to investigate the expression of 48 genes in the loin muscle of Dzhalginsky Merino sheep bred in Russia. Results: genes GAPDH, PYGM, CAST, ATP5G1, CAPN3, SOD1, VEGFA, CALM2, YWHAZ, ASIP, MYOD1, CAPN1, GHR, OXTR, BEGAIN, SLC2A3, and SS18L2 showed the highest expression. The group of genes with a medium level of expression included ATOX1, BAMBI, TLR6, IGF2, FOS, FST, GGTA2P, C-MET, FGF5, ACVR2A, CAPN2, GH, DGAT1, and IGF1. Low levels of expression were identified for genes ABCG2, SPP2, PYGL, PPARG2, TGFB1, CXCR4, MSTN, CYP2J, LEPR, CDKN1A, IGFBP4, and SERT. Trace expression was detected in genes SST, TSHR, GDF9, FGF7 and BMP15. Significant correlation between expression level and live weight was observed for most of the investigated genes. Conclusion: our results demonstrate the feasibility of using these newly identified candidate genes as genetic markers in sheep. © 2016, Universidad de Antioquia. All rights reserved.


Trukhachev V.,Stavropol State Agrarian University | Skripkin V.,Stavropol State Agrarian University | Kvochko A.,Stavropol State Agrarian University | Kulichenko A.,Stavropol Research Anti plague Institute | And 8 more authors.
Animal Biotechnology | Year: 2016

An understanding of what effects particular genes can have on body parameters in productive animals is particularly significant for the process of marker-assisted selection. The gene of transcriptional factor CCAAT/enhancer-binding protein delta (CEBPD gene) is involved in the process of growth in animals and is known to be a promising candidate for use as a genomic marker. The structure of the CEBPD gene locus was determined using NimbleGen sequencing technology (Roche, USA). The effect of polymorphisms, which were identified using the aforementioned technology, was investigated in 30 rams of the Manych Merino sheep breed. Twenty-two single nucleotide polymorphisms (SNP) were detected in the CEBPD gene locus. Significantly, two SNPs, namely, g.315T>G and g.327C>T, have been identified for the first time. It was demonstrated that the complex of linked SNPs, consisting of g.301A>T, g.426T>C, and g.1226T>C, had a negligible effect on body parameters in Manych Merino sheep. Animals with the heterozygous type of SNP g.1142C>T exhibited changes solely in the chest and croup width. The newly discovered SNP g.327C>T was proven to have a negative effect on live weight and body size (p < 0.05) in Manych Merino sheep. Sheep with the heterozygous type of g.562G>A and g.3112C>G SNP complex showed an increase in live weight and dimensions (p < 0.05) compared with those of wild homozygous type. Consequently, SNPs g.327C>T, g.562G>A, and g.3112C>G in the CEBPD gene locus can be successfully used as markers in sheep breeding. Future research will evaluate the influence of the aforementioned SNPs on slaughter indicators for sheep meat production. © 2016, Copyright © Taylor & Francis Group, LLC.


Trukhachev V.I.,Stavropol State Agrarian University | Krivoruchko A.Y.,Stavropol State Agrarian University | Skripkin V.S.,Stavropol State Agrarian University | Kvochko A.N.,Stavropol State Agrarian University | And 7 more authors.
Russian Journal of Genetics | Year: 2016

This paper investigates the structure of androgen receptor gene (AR) in the Russian breed of Dzhalginsky Merino sheep. Polymorphisms of the gene were detected using NimbleGen sequencing technology (Roche, United States). Eight single nucleotide polymorphisms (SNPs) and two insertions were detected. Five of these SNPs (c.335T>G, c.339G>A, c.342T>C, c.2491-327T>A, and c.2491-325A>T) and both insertions were identified for the first time. Three SNPs and the insertions are located in the coding part of exon. Insertion c.336_337 is found in most of the animals of this breed and can be used as a genomic marker of the breed. Animals with mutant variant of SNP c.1496+15T>C have significantly lower live weight and body size compared with the wild type genotype. This SNP can be used as a genetic marker of meat production in marker-assisted selection. © 2016, Pleiades Publishing, Inc.


Trukhachev V.,Stavropol State Agrarian University | Belyaev V.,Stavropol State Agrarian University | Kvochko A.,Stavropol State Agrarian University | Kulichenko A.,Stavropol Research Anti plague Institute | And 13 more authors.
Genetika | Year: 2016

One of the new promising candidate genes defining productive qualities of sheep is MEF2B. Protein from the MEF2 group encoded by it affects the production of myostatin and the expression of the genes responsible for the growth of skeletal muscle fibers. Thus, the knowledge of the MEF2B gene structure is important for genomic selection. We have studied the structure of the MEF2B gene at sheep of Severokavkazskaya breed bred in Russia. To detect alleles we use NimbleGen sequencing technology by Roche (USA). As a result, it was revealed 14 single nucleotide polymorphisms (SNP) at the given breed. The discovered SNPare located in not coding areas. From them 7 polymorphisms are in the area of 5' upstream gene in loci: c.-1713, c.-1319, c.-839, c.-321, c.-246, c.-161, c.-3; 6 polymorphisms are in introns, loci: c.55-51, c.258+312, c.258+380, c.259-52, c.452+95, c.452+103, 1 SNP is in 3' downstream gene, c.*252. Two of the identified SNPs are significantly connected with high indices of meat productivity: c.55-51 and c.259-52. At the same time it was not possible to find out the impact on productivity of c.-1713 polymorphism. Our investigation is a base of next research of affection of different MEF2B gene alleles on meat quality and can be used to prepare PCR test-system for genomic selection.


Kuleshov K.V.,Federal Budget Institute of Science Central Research Institute for Epidemiology | Kostikova A.,InsideDNA Ltd | Pisarenko S.V.,Stavropol Research Anti Plague Institute | Kovalev D.A.,Stavropol Research Anti Plague Institute | And 8 more authors.
Infection, Genetics and Evolution | Year: 2016

Cholera is a water-borne, severe enteric infection essentially caused by toxigenic strains of Vibrio cholera O1 and O139 serogroups. An outbreak of cholera was registered during May–July 2011 in Mariupol, Ukraine, with 33 cholera cases and 25 carriers of cholera. Following this outbreak, the toxigenic strain of V. cholerae 2011EL-301 was isolated from seawater in the recreation area of Taganrog city on the territory of Russia. The aim of our study was to understand genomic features of Mariupol isolates as well as to evaluate hypothesis about possible interconnection between the outbreak of cholera in Mariupol and the single case of isolation of V. cholerae from the Sea of Azov in Russia. Mariupol isolates were phenotypically characterized and subsequently subjected to whole genome sequencing procedure. Phylogenetic analysis based on high-quality SNPs of V. cholera O1 El Tor isolates of the 7th pandemic clade from different regions showed that clinical and environmental isolates from Mariupol outbreak were attributable to a unique phylogenetic clade within wave 3 of V. cholera O1 El Tor isolates and characterized by six clade-specific SNPs. Whereas Taganrog isolate belonged to distantly related clade which allows us to reject the hypothesis of transmission the outbreak strain of V. cholerae O1 from Ukraine to Russia in 2011. Mariupol isolates shared a common ancestor with Haiti\Nepal-4\India clade indicating that outbreak progenitor strain most likely originated in the South Asia region and later was introduced to Ukraine. Moreover, genomic data both based on hqSNPs and similarity of virulence-associated mobile genomic elements of Mariupol isolates suggests that environmental and clinical isolates are a part of joint outbreak which confirms the role of contaminated domestic sewage, as an element of the complex chain of infection spread during cholera outbreak. In general, the genome-wide comparative analysis of both genes and genomic regions of epidemiological importance indicates accessory of this isolates to ‘new’ clone of toxigenic multiple drug resistance atypical variant of V. cholerae O1 El Tor. © 2016 Elsevier B.V.


Tsapko N.V.,Stavropol Research Anti Plague Institute
Zoologicheskii Zhurnal | Year: 2016

The results and analysis of the materials obtained during field research in the eastern part of Stavropol region in 2011-2014 are considered. The critical assessment of some literary data on the distribution of the giant blind mole rat was made. The current number of the animal is evaluated; its habitats and limiting factors of its distribution are described. Giant blind mole rat prefers areas of fixed sands of eastern Stavropol region, where the number and distribution are uneven and determined by the existence and preservation of suitable biotopes. The total area occupied by this rodent within the territory studied is about 2300 km2. The densest settlements of giant blind mole rat were found in the northern part of Irgaklinsky sands.

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