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Olomouc, Czech Republic

Bardon J.,Statni Veterinarni Ustav Olomouc | Vagnerova I.,Ustav Mikrobiologie Lekarske Fakulty UP v Olomouci | Kolar M.,Ustav Mikrobiologie Lekarske Fakulty UP v Olomouci
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2012

The bacterium Clostridium difficile is pathogenic for both humans and animals. This feared nosocomial infectious agent is increasingly more important in the community as well. Like in humans, the gastrointestinal tract is infected in animals. The bacterium may be transmitted from animals to humans via the food chain (e.g. pork meat) or by direct contact. Identical human and animal isolates of C. difficile have been reported in several studies. The article presents results from a small local study aimed at detecting C. difficile and its toxins in the District of Olomouc, Czech Republic, by 3 different methods. Source


Bardon J.,Ustav mikrobiologie | Kohnova I.,Usek Mikrobiologie | Prokes Z.,Infekcni Oddeleni | Skalka P.,Statni Veterinarni Ustav Olomouc | Bzdil J.,Statni Veterinarni Ustav Olomouc
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2011

Bacterial zoonoses still represent a serious medical problem. One of the less frequent but severe zoonoses is brucellosis caused by the bacterium Brucella melitensis. The presented case report describes relapsing imported brucellosis in a young male. In addition to four serological tests, the diagnosis was confirmed by direct detection of the pathogen in blood culture. The isolate of Brucella melitensis was identified using the MALDI-TOF BioTyper method and subsequently also by PCR. Source


Bardon J.,Statni Veterinarni Ustav Olomouc | Harna J.,Statni Veterinarni Ustav Olomouc | Pijacek M.,Statni Veterinarni Ustav Olomouc
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2013

Parasites of the family Anisakidae cause enteric parasitic zoonoses developing after consumption of inadequately cooked marine fish. Cases of such diseases are reported mainly from Japan or other countries where raw or uncooked fish are traditionally consumed. The presented short communication briefly reports detection of larvae of Pseudoterranova spp., parasites of the family Anisakidae, in a fresh chilled angler-fish (Lophius piscatorius) bought at a retail store in the Czech Republic. Source


Bardon J.,Statni Veterinarni Ustav Olomouc | Ondruskova J.,Statni Veterinarni Ustav Olomouc | Oslikova M.,Statni Veterinarni Ustav Olomouc | Vyroubalova S.,Statni Veterinarni Ustav Olomouc
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2012

Background: To assess current microbiological risks of raw cow's milk. The presented work reports the prevalence of selected bacteria on Czech dairy farms in 2010 and compares the results with a similar study in 2002. Material and methods: The prevalence of bacteria was studied by culture methods using milk filters made from non-woven fabric collected from dairy farms throughout the year 2010. Together 260 filters from 65 dairy farms were tested. The prevalence of Campylobacter spp., Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O 157 and Salmonella spp. in filters was tested. In staphylococci, genes encoding enterotoxin-production were studied. In Campylobacter spp., resistance to antibiotics was ascertained. Results: In 2010, the prevalence rates of selected bacteria on farms were as follows: Campylobacter spp. 3%, Escherichia coli O 157 0.4%, Salmonella spp. 0.8%, Listeria monocytogenes 10% and Staphylococcus aureus 31%. In 12% of the tested filters, S. aureus with the genetic make-up for enterotoxin production was isolated. Antibiotic resistance in Campylobacter spp. isolated from dairy farms is different from that in human or poultry isolates. Conclusion: Raw cow's milk contains bacteria capable of causing human alimentary tract diseases. The above microbiological risks for consumers are eliminated by proper storage conditions (below 10°C) and heat treatment (boiling) of raw milk. Source


Bardon J.,Statni Veterinarni Ustav Olomouc | Pijacek M.,Statni Veterinarni Ustav Olomouc | Harna J.,Statni Veterinarni Ustav Olomouc | Bz Dil J.,Statni Veterinarni Ustav Olomouc | And 2 more authors.
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2012

Brucellosis is a rare but serious bacterial zoonosis. Officially, the Czech Republic is among countries that are free from brucellosis in livestock. In the country, sporadic imported human infections may occur, caused by e.g. Brucella melitensis. In wild hare populations, however, rare cases of infection caused by Brucella suis are still observed, potentially threatening humans. The short communication reports two cases of hare brucellosis in Moravia, Czech Republic, and experiences with isolate identification by the MALDI-TOF method. Source

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