Statni Veterinarni Ustav

Olomouc, Czech Republic

Statni Veterinarni Ustav

Olomouc, Czech Republic

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Tarabova R.,Ustav mikrobiologie LF UP v Olomouci a FN Olomouc | Bardon J.,Statni veterinarni ustav
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2016

MALDI-TOF MS is a method enabling rapid identification of bacteria. This is also important for early initiation of adequate antibiotic therapy in patients with infections. In this review, various methods for direct detection of bacteria in clinical specimens are described. The fundamental part deals with direct identification of bacteria from positive blood cultures. Attention is also paid to identification of bacteria from urine and cerebrospinal fluid. Finally, reliability of the methods is mentioned in comparison with conventional methods.


Raclavsky V.,Ustav Mikrobiologie | Bardon J.,Statni veterinarni ustav | Petrzelova J.,Ustav Mikrobiologie | Prochazkova P.,Ustav Mikrobiologie | And 7 more authors.
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2016

Current standards of care for cystic fibrosis (CF) patients lack unequivocal recommendations concerning the duration of primary culture of bacteriological samples. With the exception of Burkholderia cepacia (5 days), the minimum recommended duration of primary culture varies between 48 and 72 hours. Our aim was to evaluate the effect of an extended 10-day period of primary culture in a humid chamber in samples acquired from the respiratory tract of patients suffering from CF. Compared to standard culture, prolonged culture in a humid chamber yielded 1.85 times more isolates of pathogenic species in pharyngeal swabs (76 versus 41 isolates) and 1.4 times more isolates in sputum samples (116 versus 82), but only 1.14 times more isolates in nasal swabs (25 versus 22). Prolonged culture was most beneficial for Achromobacter spp. (6 versus 0), Stenotrophomonas maltophilia (16 versus 5) and Pseudomonas aeruginosa (69 versus 49), whereas there was little or no benefit at all for Staphylococcus aureus (87 versus 73) and Moraxella catarrhalis (10 versus 10). Therefore, prolonged culture in a humid chamber may definitely be recommended for pharyngeal swabs and sputum samples obtained from patients suffering from CF to achieve the maximum recovery rate of pathogenic bacteria, in particular non-fermenting Gram-negative rods.


Kolackova I.,Vyzkumny Ustav Veterinarniho Lekarstvi | Stromerova N.,Statni Veterinarni Ustav | Bardon J.,Statni Veterinarni Ustav | Pudova V.,Ustav Mikrobiologie | And 2 more authors.
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2015

Objectives: Molecular epidemiology is a field that uses results of typing techniques to obtain information on detailed characterization of bacterial strains for determining the identity, similarity or difference in bacteria of the same genus, species or serotype. Nowadays, the most commonly used methods are based on monitoring differences in bacterial genotypes. However, most of these techniques are time-consuming and costly. A method increasingly used in routine microbiological testing is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which is based on analysis of the bacterial proteome. It is mainly used for rapid and accurate classification of bacteria into genera and species. The aims were to assess the potential use of this method for typing of Campylobacter below the species level and to apply these results in epidemiological investigations. Material and Methods: The study comprised 39 strains of Campylobacter jejuni isolated from food (16) and humans (23). Macrorestriction fragment profiling by pulsed-field gel electrophoresis (PFGE) and simultaneous protein profile analysis using MALDI-TOF MS were performed for all tested strains. Results: Similar pulse profiles were found among isolates originating from the same outbreak or repeatedly collected from a single patient. The same pulse profiles were also detected in strains of unknown relationship but sharing the same place of origin and year of isolation. The comparison of dendrograms from both analyses showed that strains identified as identical by PFGE appeared in the same subgroups in dendrograms obtained by MALDI-TOF MS, the only exception being isolates repeatedly collected from a single patient. Conclusion: The results suggest that confirmation of the identity or similarity of strains in accordance with the established epidemiological facts has not been clearly demonstrated using MALDI-TOF MS.


Koudela B.,University of Veterinary And Pharmaceutical Sciences Brno | Harna J.,Statni Veterinarni Ustav | Pijacek M.,Statni Veterinarni Ustav
Klinicka Mikrobiologie a Infekcni Lekarstvi | Year: 2011

The nematode Trichinella spp. is the etiological agent of trichinellosis, a zoonotic parasitic disease. Many carnivorous and omnivorous animal species may become infected with Trichinella spp., including humans. Trichinella spp. is transmitted orally through consumption of raw or undercooked meat. There is a general agreement that animals do not get sick following infection. However, the course of infection in humans includes disease that can range from subclinical to fatal. Because of its role in human disease, there are increasing global requirements for reliable diagnostic and control methods for Trichinella in food animals to ensure meat safety. This review article describes the biology and history of human and animal trichinellosis in the Czech Republic, and recommended test methods as well as modified and optimized procedures that are used in meat inspection programmes.


PubMed | Statni veterinarni ustav
Type: Journal Article | Journal: Klinicka mikrobiologie a infekcni lekarstvi | Year: 2012

To assess current microbiological risks of raw cows milk. The presented work reports the prevalence of selected bacteria on Czech dairy farms in 2010 and compares the results with a similar study in 2002.The prevalence of bacteria was studied by culture methods using milk filters made from non-woven fabric collected from dairy farms throughout the year 2010. Together 260 filters from 65 dairy farms were tested. The prevalence of Campylobacter spp., Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O 157 and Salmonella spp. in filters was tested. In staphylococci, genes encoding enterotoxin-production were studied. In Campylobacter spp., resistance to antibiotics was ascertained.In 2010, the prevalence rates of selected bacteria on farms were as follows: Campylobacter spp. 3%, Escherichia coli O 157 0.4%, Salmonella spp. 0.8%, Listeria monocytogenes 10% and Staphylococcus aureus 31%. In 12% of the tested filters, S. aureus with the genetic make-up for enterotoxin production was isolated. Antibiotic resistance in Campylobacter spp. isolated from dairy farms is different from that in human or poultry isolates.Raw cows milk contains bacteria capable of causing human alimentary tract diseases. The above microbiological risks for consumers are eliminated by proper storage conditions (below 10 degrees C) and heat treatment (boiling) of raw milk.


PubMed | Statni veterinarni ustav
Type: Journal Article | Journal: Klinicka mikrobiologie a infekcni lekarstvi | Year: 2012

Brucellosis is a rare but serious bacterial zoonosis. Officially, the Czech Republic is among countries that are free from brucellosis in livestock. In the country, sporadic imported human infections may occur, caused by e.g. Brucella melitensis. In wild hare populations, however, rare cases of infection caused by Brucella suis are still observed, potentially threatening humans. The short communication reports two cases of hare brucellosis in Moravia, Czech Republic, and experiences with isolate identification by the MALDI-TOF method.

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