StatistiCal BV

Wassenaar, Netherlands

StatistiCal BV

Wassenaar, Netherlands
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Polhuis K.C.M.M.,The Dutch Beer Institute | Polhuis K.C.M.M.,Wageningen University | Wijnen A.H.C.,The Dutch Beer Institute | Sierksma A.,The Dutch Beer Institute | And 3 more authors.
Nutrients | Year: 2017

With ageing, there is a greater risk of dehydration. This study investigated the diuretic effect of alcoholic beverages varying in alcohol concentration in elderly men. Three alcoholic beverages (beer (AB), wine (AW), and spirits (S)) and their non-alcoholic counterparts (non-alcoholic beer (NAB), non-alcoholic wine (NAW), and water (W)) were tested in a diet-controlled randomized crossover trial. For the alcoholic beverages, alcohol intake equaled a moderate amount of 30 g. An equal volume of beverage was given for the non-alcoholic counterpart. After consumption, the urine output was collected every hour for 4 h and the total 24 h urine output was measured. AW and S resulted in a higher cumulative urine output compared to NAW and W during the first 4 h (effect size: 0.25 mL p < 0.003, effect size: 0.18 mL, p < 0.001, respectively), but not after the 24h urine collection (p > 0.40, p > 0.10). AB and NAB did not differ at any time point (effect size: −0.02 mL p > 0.70). For urine osmolality, and the sodium and potassium concentration, the findings were in line. In conclusion, only moderate amounts of stronger alcoholic beverages, such as wine and spirits, resulted in a short and small diuretic effect in elderly men. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.


Calame W.,StatistiCal BV | Thomassen F.,Clinical Trial Management | Hull S.,Leatherhead Food Research | Viebke C.,Flavours | Siemensma A.D.,Kerry Group Nutrition Technical Center
Appetite | Year: 2011

In the present study a potential satiating effect by two blends of gum arabic (EmulGold ® (EG) and PreVitae ® (PV)) was investigated in healthy humans applying a regression analysis on the change of values throughout the interval of the study. Two studies were thus conducted: a feasibility study using doses between 10 and 40g and a dose-finding study of 5 or 10g of only EG. The gums were dissolved in 250ml of water (negative control). In both studies energy intake was determined 3h after consumption, while Visual Analogue Scale (VAS) scores were recorded every 30min from the time of consumption onwards. At doses of 40g both EG and PV yielded a significant reduction in energy intake of more than 100 and 200kcal, respectively. At doses of 10 or 20g the reduction in energy intake amounted to more than 100kcal for both. The second study demonstrated a significant reduction in caloric intake of more than 60kcal at doses of 5 and 10g of EG. With respect to the subjective perception of satiety, VAS scores revealed a significant increase as compared to the negative control of all doses of both gums. The regression analysis was sensitive in identifying not only the intensity of the perception during the time interval of the study but also the change in this intensity over time. The results of this study show that both blends of gum arabic are able to decrease the caloric intake significantly 3h after consumption, and increase subjective ratings of feeling satiated, and could therefore be used in a dietary approach to control body weight development. © 2011 Elsevier Ltd.


Schaafsma A.,FrieslandCampina | Deurenberg P.,Nutrition Consultant | Calame W.,StatistiCal BV | Van Den Heuvel E.G.H.M.,FrieslandCampina | And 8 more authors.
British Journal of Nutrition | Year: 2013

Nutrition is a well-known factor in the growth, health and development of children. It is also acknowledged that worldwide many people have dietary imbalances resulting in over- or undernutrition. In 2009, the multinational food company FrieslandCampina initiated the South East Asian Nutrition Survey (SEANUTS), a combination of surveys carried out in Indonesia, Malaysia, Thailand and Vietnam, to get a better insight into these imbalances. The present study describes the general study design and methodology, as well as some problems and pitfalls encountered. In each of these countries, participants in the age range of 0·5-12 years were recruited according to a multistage cluster randomised or stratified random sampling methodology. Field teams took care of recruitment and data collection. For the health status of children, growth and body composition, physical activity, bone density, and development and cognition were measured. For nutrition, food intake and food habits were assessed by questionnaires, whereas in subpopulations blood and urine samples were collected to measure the biochemical status parameters of Fe, vitamins A and D, and DHA. In Thailand, the researchers additionally studied the lipid profile in blood, whereas in Indonesia iodine excretion in urine was analysed. Biochemical data were analysed in certified laboratories. Study protocols and methodology were aligned where practically possible. In December 2011, data collection was finalised. In total, 16744 children participated in the present study. Information that will be very relevant for formulating nutritional health policies, as well as for designing innovative food and nutrition research and development programmes, has become available. Copyright © The Authors 2013A.


Pombo-Rodrigues S.,Nutrition Research | Calame W.,StatistiCal BV | Re R.,Nutrition Research
International Journal of Food Sciences and Nutrition | Year: 2011

The aim of the present work was to investigate the effects of eggs consumed for lunch on satiety, satiation and subsequent energy intake at the next meal. Thirty-one healthy male and female subjects participated in a randomized, three-way, crossover study. Following consumption of a standard breakfast, participants were asked to consume three isocaloric test lunches: omelette, jacket potato and chicken sandwich. Subjective measures of satiety were recorded using visual analog scales at regular intervals throughout the day. Energy intake at the next meal was assessed 4 h after lunch with an ad libitum meal. The egg lunch showed a significantly stronger satiating effect compared with the jacket potato meal. No effect on energy intake was seen. These data indicate that consumption of an omelette meal consumed at lunch could increase satiety to a greater extent than a carbohydrate meal and may facilitate reduction of energy consumption between meals. © 2011 Informa UK, Ltd.


Ijsselstijn L.,Erasmus Medical Center | Papma J.M.,Erasmus Medical Center | Dekker L.J.M.,Erasmus Medical Center | Calame W.,StatistiCal BV | And 6 more authors.
Proteomics | Year: 2013

We have explored proteins related to mild cognitive impairment (MCI). The serum proteome of 35 amnestic MCI patients and 35 cognitively healthy persons was investigated by LC MS. We identified 108 differentially expressed peptides between MCI patients and controls, belonging to 39 proteins. Eight proteins were selected for further investigation by quantitative protein measurements using a MRM assay; apolipoprotein E, carboxypeptidase N subunit 2, complement factor B (CFAB), galectin-3 binding protein (LG3BP), lumican, serum amyloid A-4 protein (SAA4), serum amyloid P-component, and sex hormone binding globulin. Results of the quantitative protein measurements showed significantly decreased levels of carboxypeptidase N subunit 2, CFAB, LG3BP, SAA4, and serum amyloid P-component in serum from amnestic MCI patients compared with cognitive healthy controls (two-sided t-test; p < 0.05). Apolipoprotein E and lumican showed no significant difference in protein levels, sex hormone binding globulin could not be quantified since the MRM assay did not reach the required sensitivity. A model based on the three most significantly decreased proteins (CFAB, LG3BP, and SAA4) showed a sensitivity and specificity of 73 and 66%, respectively, for the initial sample set. A small external validation set yielded 77% sensitivity and 75% specificity. © 2013 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim.


De Costa D.,Erasmus Medical Center | Broodman I.,Erasmus Medical Center | Calame W.,StatistiCal BV | Stingl C.,Erasmus Medical Center | And 8 more authors.
PLoS ONE | Year: 2014

Late diagnosis of lung cancer is still the main reason for high mortality rates in lung cancer. Lung cancer is a heterogeneous disease which induces an immune response to different tumor antigens. Several methods for searching autoantibodies have been described that are based on known purified antigen panels. The aim of our study is to find evidence that parts of the antigen-binding-domain of antibodies are shared among lung cancer patients. This was investigated by a novel approach based on sequencing antigen-binding- fragments (Fab) of immunoglobulins using proteomic techniques without the need of previously known antigen panels. From serum of 93 participants of the NELSON trial IgG was isolated and subsequently digested into Fab and Fc. Fab was purified from the digested mixture by SDS-PAGE. The Fab containing gel-bands were excised, tryptic digested and measured on a nano-LC-Orbitrap-Mass- spectrometry system. Multivariate analysis of the mass spectrometry data by linear canonical discriminant analysis combined with stepwise logistic regression resulted in a 12-antibody-peptide model which was able to distinguish lung cancer patients from controls in a high risk population with a sensitivity of 84% and specificity of 90%. With our Fab-purification combined Orbitrap-mass-spectrometry approach, we found peptides from the variable-parts of antibodies which are shared among lung cancer patients. © 2014 de Costa et al.


Sekartini R.,University of Indonesia | Wiguna T.,University of Indonesia | Bardosono S.,University of Indonesia | Novita D.,University of Indonesia | And 3 more authors.
British Journal of Nutrition | Year: 2013

Glycaemic response to dietary carbohydrates might have an impact on cognitive performance. The present study investigated the effects of growing-up milks (GUM) with isomaltulose and extra minerals and vitamins or lower protein content on cognitive parameters in children aged 5-6 years. In a blinded, partly randomised, controlled, cross-over study, four GUM were provided, each taken over 14 d (2 × 200 ml/d): standard (Std) GUM; Std GUM+5 g isomaltulose (Iso-5 GUM); Iso-5 GUM with 26 % less protein (Iso-5 LP GUM); Std GUM with 2·5 g isomaltulose and extra Mg, Zn, Se, D3, B1, B2, B12, folic acid and choline (Iso-2·5 GUM). At test days, when GUM replaced breakfast, repeated (0, 60, 120 and 180 min post-dose) cognitive tasks were performed (picture presentation, simple reaction time, digit vigilance, choice reaction time, spatial and numeric working memory and picture recognition). Task performance of all subjects (n 50) worsened over the morning. Best performance was seen on isomaltulose GUM, most notably at 180 min. Iso-2·5 GUM showed best performance on several parameters of attention and memory, Iso-5 GUM performed best on parameters of memory and Iso-5 LP GUM was positively associated with parameters of attention but less with memory. Std GUM showed only a benefit on one attention and one memory task. Thus, isomaltulose-enriched GUM positively affected parameters of attention and memory at 180 min post-dose when compared with Std GUM. Extra minerals and vitamins seem beneficial, whereas lowering protein content might improve attention in particular. Copyright © The Authors 2013.


Broker M.E.E.,Erasmus Medical Center | Lalmahomed Z.S.,Erasmus Medical Center | Roest H.P.,Erasmus Medical Center | van Huizen N.A.,Erasmus Medical Center | And 5 more authors.
PLoS ONE | Year: 2013

Introduction:For both patients and the outpatient clinic the frequent follow-up visits after a resection of colorectal cancer (CRC) are time consuming and due to large patient numbers expensive. Therefore it is important to develop an effective non-invasive test for the detection of colorectal liver metastasis (CRLM) which could be used outside the hospital. The urine proteome is known to provide detailed information for monitoring changes in the physiology of humans. Urine collection is non-invasive and urine naturally occurring peptides (NOPs) have the advantage of being easily accessible without labour-intensive sample preparation. These advantages make it potentially useful for a quick and reliable application in clinical settings. In this study, we will focus on the identification and validation of urine NOPs to discriminate patients with CRLM from healthy controls.Materials and Methods:Urine samples were collected from 24 patients with CRLM and 25 healthy controls. In the first part of the study, samples were measured with a nano liquid chromatography (LC) system (Thermo Fisher Scientific, Germaring, Germany) coupled on-line to a hybrid linear ion trap/Orbitrap mass spectrometer (LTQ-Orbitrap-XL, Thermo Fisher Scientific, Bremen, Germany). A discovery set was used to construct the model and consecutively the validation set, being independent from the discovery set, to check the acquired model. From the peptides which were selected, multiple reaction monitoring (MRM's) were developed on a UPLC-MS/MS system.Results:Seven peptides were selected and applied in a discriminant analysis a sensitivity of 84.6% and a specificity of 92.3% were established (Canonical correlation:0.797, Eigenvalue:1.744, F:4.49, p:0.005). The peptides AGPP(-OH)GEAGKP(-OH)GEQGVP(-OH)GDLGA P(-OH)GP and KGNSGEP(-OH)GAPGSKGDTGAKGEP(-OH)GPVG were selected for further quantitative analysis which showed a sensitivity of 88% and a specificity of 88%.Conclusion:Urine proteomic analysis revealed two very promising peptides, both part from collagen type 1, AGPP(-OH)GEAGKP(-OH)GEQGVP(-OH)GDLGAP(-OH)GP and KGNSGEP(-OH)GAPGSKGDTGAKGEP(-OH)GPVG which could detect CRLM in a non-invasive manner. © 2013 Bröker et al.


PubMed | StatistiCal BV
Type: Journal Article | Journal: Appetite | Year: 2011

In the present study a potential satiating effect by two blends of gum arabic (EmulGold() (EG) and PreVitae() (PV)) was investigated in healthy humans applying a regression analysis on the change of values throughout the interval of the study. Two studies were thus conducted: a feasibility study using doses between 10 and 40 g and a dose-finding study of 5 or 10 g of only EG. The gums were dissolved in 250 ml of water (negative control). In both studies energy intake was determined 3 h after consumption, while Visual Analogue Scale (VAS) scores were recorded every 30 min from the time of consumption onwards. At doses of 40 g both EG and PV yielded a significant reduction in energy intake of more than 100 and 200 kcal, respectively. At doses of 10 or 20 g the reduction in energy intake amounted to more than 100 kcal for both. The second study demonstrated a significant reduction in caloric intake of more than 60 kcal at doses of 5 and 10 g of EG. With respect to the subjective perception of satiety, VAS scores revealed a significant increase as compared to the negative control of all doses of both gums. The regression analysis was sensitive in identifying not only the intensity of the perception during the time interval of the study but also the change in this intensity over time. The results of this study show that both blends of gum arabic are able to decrease the caloric intake significantly 3 h after consumption, and increase subjective ratings of feeling satiated, and could therefore be used in a dietary approach to control body weight development.

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