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Olomouc, Czech Republic

Polisenskaa I.,R.O.S.A. | Pfohl-Leszkowicz A.,National Graduate School of Agronomy, Toulouse | Hadjeba K.,National Graduate School of Agronomy, Toulouse | Dohnal V.,Mendel University in Brno | And 4 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2010

The aims of the study were to obtain information about the occurrence of ochratoxin A (OTA) and citrinin (CIT) in cereals harvested in the Czech Republic and to compare two analytical procedures for detecting OTA. A total of 34 cereal samples, including two matrix reference materials (R-Biopharm, Germany), were analysed. The results were compared with the limit for raw cereal grains used as a foodstuff according to Commission Regulation No. 1881/2006, which allows a maximum OTA level of 5 μg kg-1. Compared were two methods based on the high-performance liquid chromatography principle, one using the immunoaffinity columns OchraTest™ (VICAM) and the second based on solvent partition (PART), both followed by fluorescence detection. The highest OTA contents were found in two barley samples. According to the method employed, the results for the first sample (malting barley) were VICAM = 31.43 μg kg-1 and PART = 44.74 μg kg-1. For the second sample (feeding barley) they were VICAM = 48.63 μg kg-1 and PART = 34.40 μg kg-1. Two samples of bread wheat had an OTA content approaching the legal limit (VICAM = 4.71 μg kg-1 and PART = 6.03 μg kg-1; VICAM = 4.12 μg kg-1 and PART = 3.95 μg kg-1). CIT was analysed using the PART method only, and its highest content (93.64 μg kg-1) was found for the malting barley sample with high OTA content (44.74 μg kg-1 as analysed using PART). © 2010 Taylor & Francis. Source

Bardon J.,Palacky University | Bardon J.,State Veterinary Institute Olomouc | Husickova V.,Palacky University | Chroma M.,Palacky University | Kolar M.,Palacky University
Journal of Food Protection | Year: 2013

Resistance of bacteria to antibiotics is a global medical problem requiring close cooperation between veterinary and human physicians. Raw materials and foods of animal origin may be not only a source of pathogenic bacteria causing alimentary tract infections but also a source of bacteria with a dangerous extent of resistance to antibiotics, potentially entering the human food chain. This article presents results of the first study in the Czech Republic detecting the presence of Enterobacteriaceaeproducing extended-spectrum β-lactamases (ESBLs) in swabs collected in slaughterhouses from surfaces of healthy animal carcasses. In 2012, swabs taken from pig (n = 166) and cattle (n = 140) carcass surfaces were analyzed. In 17% of 53 studied slaughterhouses, ESBL-producing Escherichia coli strains were isolated. ESBLs were found in 11 and 4% of porcine and bovine samples, respectively. Swabs collected from pigs yielded 18 ESBL-producing E. coli strains. The bla genes were found to encode production of CTX-M-1 group enzymes in 16 strains, SHV in one case, and both CTX-M-1-like and TEM in another case. In swabs taken from cattle, five ESBL-producing E. coli strains were isolated. In three cases, the bla genes for CTX-M-1-like production were identified; in two cases, genes for both CTX-M-1-like and TEM production were found. The similarity/identity of ESBL-positive isolates was compared by pulsed-field gel electrophoresis. This is the first report and characterization of the presence and nature of ESBL-producing E. coli in swabs collected from surfaces of healthy pig and cattle carcasses in slaughterhouses in the Czech Republic. Copyright © International Association for Food Protection. Source

Veverkova L.,Palacky University | Hradilova S.,Palacky University | Milde D.,Palacky University | Panacek A.,Palacky University | And 5 more authors.
Spectrochimica Acta - Part B Atomic Spectroscopy | Year: 2014

This study examined recoveries of silver determination in animal tissues after wet digestion by inductively coupled plasma mass spectrometry. The composition of the mineralization mixture for microwave assisted digestion was optimized and the best recoveries were obtained for mineralization with HNO3 and addition of HCl promptly after digestion. The optimization was performed on model samples of chicken meat spiked with silver nanoparticles and a solution of ionic silver. Basic calculations of theoretical distribution of Ag among various silver-containing species were implemented and the results showed that most of the silver is in the form of soluble complexes AgCl2 - and AgCl3 2- for the optimized composition of the mineralization mixture. Three animal tissue certified reference materials were then analyzed to verify the trueness and precision of the results. © 2014 Elsevier B.V. All rights reserved. Source

Bardon J.,State Veterinary Institute Olomouc | Bardon J.,Palacky University | Kolar M.,Palacky University | Karpiskova R.,National Institute of Public Health | Hricova K.,Palacky University
Food Control | Year: 2011

Infections caused by thermotolerant Campylobacter spp. are among the most frequent bacterial diseases from food in Europe. An important source of infection is poultry. The aim was to perform the first nationwide study on the prevalence of Campylobacter spp. at retail in the Czech Republic. Samples of chilled and frozen broiler chickens were obtained from supermarkets localized in the eight largest cities in the Czech Republic. From February to November 2009, a total of 120 samples of chilled and 120 samples of frozen poultry were collected and assessed. Qualitative and quantitative analyses were performed according to the ISO 10272-1:2006 and ISO 10272-2:2006 methods. The microdilution method was used to test the resistance of Campylobacter spp. isolates to selected antibiotics. High prevalence of Campylobacter spp. was detected in chilled poultry (75%). The prevalence was significantly lower (37%) in frozen poultry. The most prevalent species was Campylobacter jejuni detected in 70% of positive findings, followed by Campylobacter coli (18%). In 12% of cases, the samples were contaminated by both C. jejuni and C. coli. The species Campylobacter lari and Campylobacter upsaliensis were not detected. The highest resistance rates were to quinolone antibiotics and ampicillin. © 2010 Elsevier Ltd. Source

Svobodova L.,Palacky University | Bednarova D.,GeneProof A.s. | Ruzicka F.,Masaryk University | Chrenkova V.,Charles University | And 8 more authors.
Mycoses | Year: 2016

Clinical yeast isolates belonging to Candida pelliculosa, Candida utilis and Candida fabianii are difficult to distinguish in a routine mycology laboratory using common biochemical tests. The aims of this study were to determine the prevalence of C. pelliculosa, C. utilis and C. fabianii in clinical samples and to compare their minimum inhibitory concentrations (MICs) to systemic antifungals. Two hundred and forty-eight clinical yeast isolates obtained from eight large hospitals in the Czech Republic were included in this study. Identification was performed biochemically using ID 32C kit and by MALDI-TOF MS. MICs were determined using colorimetric broth dilution Sensititre YeastOne panels. From a total number of 248 isolates, 175 were identified as C. pelliculosa and 73 as C. utilis using the biochemical kit. In contrast, MALDI-TOF MS identified 222 isolates as C. fabianii, 20 as C. pelliculosa and 6 as C. utilis. The highest mean MICs were found in C. fabianii and, regardless of the studied species, in isolates from blood cultures and central venous catheters. MALDI-TOF MS revealed C. fabianii to be most prevalent in clinical samples as compared with the other studied species. Higher MIC values in C. fabianii support the importance of correct identification of this species. © 2016 Blackwell Verlag GmbH. Source

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