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Vitoria da Conquista, Brazil

Brito R.O.,State University of Southwest Bahia
Genetics and molecular research : GMR | Year: 2010

The order Thysanoptera is composed of cosmopolitan phytophagous and predaceous insects with diverse life histories, behaviors and habits. This order is currently thought to form a trichotomy with Hemiptera and Psocodea; Hemiptera and Thysanoptera are considered to be sister groups. The interrelationships within Thysanoptera remain unclear and cytotaxonomic studies are scarce in thrips. We report, for the first time, chromosomal data on seven species of thrips collected from a semi-arid region in the States of Bahia and Pernambuco (Northeast Brazil). A distinctive chromosomal pattern was observed in Thysanoptera when compared to other members within the infraclass Paraneoptera. Considerable karyotypic differences were also found within genera and species of Thysanoptera. Based on these data, we suggest that Paraneoptera forms a polyphyletic group and that Terebrantia and Tubulifera should be regarded as sister groups. The high chromosomal variability observed in Thysanoptera indicates that chromosomal rearrangements have played a key role in their speciation pathways.

Cerqueira-Silva C.B.,State University of Southwest Bahia
Genetics and molecular research : GMR | Year: 2012

Little is known about the molecular genetic diversity of most Passiflora species. We used RAPD markers to evaluate the genetic diversity of 24 genotypes of the 'sleep' passion fruit (Passiflora setacea). Twelve primers generated 95 markers, 88% of which were polymorphic. The genetic distance estimated by the complement of the Dice index ranged from 0.29 (among accessions Ps-G1 and Ps-G13) to 0.69 (among accessions Ps-G21 and Ps-G23). Genotype grouping based on the UPGMA algorithm showed considerable variability among genotypes. We conclude that P. setacea has a broad genetic base that could be exploited in breeding programs.

Cerqueira-Silva C.B.,State University of Southwest Bahia
Genetics and molecular research : GMR | Year: 2010

The genetic diversity and characteristics of commercial interest of Passiflora species make it useful to characterize wild germplasm, because of their potential use for fruit, ornamental and medicinal purposes. We evaluated genetic diversity, using RAPD markers, of 32 genotypes of Passiflora cincinnata collected from the wild in the region of Vitória da Conquista, Bahia, Brazil. Thirteen primers generated 95 polymorphic markers and only one monomorphic marker. The mean genetic distance between the genotypes estimated by the complement of the Dice index was 0.51 (ranging from 0.20-0.85), and genotype grouping based on the UPGMA algorithm showed wide variability among the genotypes. This type of information contributes to identification and conservation of the biodiversity of this species and for the identification of pairs of divergent individuals for maximum exploitation of existing variability.

This article analyzes the international sanitary conferences that were held in South America in 1873 and 1887, involving the Brazilian Empire and the Republics of Argentina and Uruguay, as an integral part of a series of similar events that took place in Europe and North America starting in the second half of the nineteenth century. The interests of the countries involved, namely trade relations and immigration from Europe - both directly affected by the epidemics - are discussed, and the repercussions of these sanitary agreements on the other countries in the Americas are indicated. The American health conventions in the late nineteenth century represented the first initiatives in the Americas to solve international public health problems.

Lemos V.A.,State University of Southwest Bahia | Dos Santos L.O.,State University of Southwest Bahia
Food Chemistry | Year: 2014

The development of a method using solid phase extraction for preconcentration and determination of mercury by cold vapour atomic absorption spectrometry is described. Hg (II) ions are sorbed on a minicolumn packed with Amberlite XAD-4 sorbent functionalised with 2-(2′-benzothiazolylazo)-p- cresol (BTAC). Then, a reducing solution was used for desorption and the transport of the analyte for subsequent detection. The assay presented a limit of detection of 0.011 μg L-1 (0.011 μg g-1, for solid samples), a limit of quantification of 0.038 μg L-1 (0.038 μg g-1, for solid samples), a precision of 0.50% (1.000 μg L-1 Hg solution) and an enrichment factor of 46. The proposed method was applied to the determination of mercury in human saliva (0.055-0.200 μg L-1). The following seafood collected in Todos os Santos Bay, Brazil was also analysed: bass (0.169-0.195 μg g-1), mullet (0.043-0.361 μg g-1), shrimp (0.075-0.374 μg g-1) and mussel (0.206-0.397 μg g-1).© 2013 Elsevier Ltd. All rights reserved.

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