State University of MaringaParana

Science, Brazil

State University of MaringaParana

Science, Brazil

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Koehnlein E.A.,Federal University of Paraná | Koehnlein E.A.,State University of Maringá | Koehnlein E.M.,State University of MaringaParana | Correa R.C.G.,State University of Maringá | And 6 more authors.
International Journal of Food Sciences and Nutrition | Year: 2016

This work compares the phenolic contents and the total antioxidant capacity of the 36 most popular Brazilian foods submitted to aqueous extraction or in vitro digestion. The purpose was to evaluate the extent by which digestion differs from the simple aqueous extraction procedures of several food matrices. After in vitro digestion, cereals, legumes, vegetables, tuberous vegetables, chocolates and fruits showed higher phenolic contents and higher antioxidant activities than those obtained by aqueous extraction. Contrarily, the digestion caused a reduction in the phenolic contents and antioxidant activities of beverages (red wine, coffee and yerba mate). Our results suggest that the phenolics of food groups with solid and complex matrix are protected against enzymatic action and alteration in pH during the digestion, what does not occur in liquid food matrices such as the beverages. This fact would overestimate the antioxidant activities of beverages submitted solely to aqueous extraction. © 2016 Informa UK Limited, trading as Taylor & Francis Group.


Fernandes J.I.M.,Federal University of Paraná | Santos T.C.,State University of MaringaParana | Kaneko I.N.,State University of MaringaParana | Horn D.,Federal University of Paraná | And 2 more authors.
Revista Brasileira de Ciencia Avicola | Year: 2016

Adverse environmental conditions during rearing may negatively affect productivity and meat quality of the modern fast-growing broiler strains. Temperature manipulation during sensitive embryonic development periods may affect broilers’ physiological responses to environmental conditions during rearing on commercial farms. The objective of this trial was to evaluate the effect of temperature manipulation during incubation and breeder age on the meat quality of male and female broilers submitted to heat stress during the preslaughter period. In this experiment, 1280 broiler chicks were distributed according to a completely randomized experimental design in a 2x2x2 factorial arrangement. Treatments consisted of two breeder ages (30 and 60 weeks), two temperature programs applied in the last four days of incubation (standard or high temperature), and sex (male and female). Birds were submitted to 32 °C for 48 hours before slaughter, on day 46.Meat quality parameters (pH, temperature, color, and weight loss) were evaluated. There was significant effect (p<0.05) of sex on meat redness (a*) and fat percentage, with females presenting higher values than males in both measurements. Males incubated at the higher temperature presented higher (p<0.05) meat weight loss by pressure than females. The thermal manipulation applied during the final stage of incubation did not affect the meat yield or meat quality of broilers submitted to heat stress. © 2016, Fundacao APINCO de Ciencia e Tecnologia Avicolas. All rights reserved.


Sfaciotte R.A.P.,Federal University of Paraná | Bordin J.T.B.,State University of MaringaParana | Vignoto V.K.C.,State University of MaringaParana | Munhoz P.M.,State University of MaringaParana | And 5 more authors.
American Journal of Animal and Veterinary Sciences | Year: 2015

Otitis is one of the most frequent infections in dogs. This is attributed to the misuse of drugs enabling generation of multi-resistant micro-organisms. The emergence of multiresistant bacterial strains in veterinary medicine is a reality that must be studied and evaluated by the professionals. The objective of this study was to isolate and evaluate the antimicrobial susceptibility of bacterial pathogens of otitis in dogs. Otologic swabs were collected from 36 dogs with clinical otitis. 41 bacterial strains were isolated and antimicrobial susceptibility tests were performed by disk diffusion method with 34 antimicrobial agents. Presence of the resistance gene mecA of Staphylococcus was examined for 22 strains of staphylococci by PCR. A total of 1108 ratings antimicrobial agents were performed. The percentage of drug resistance was 34.66% (n = 384) of the assessments with partial or total resistance. Major bacterial pathogens were Staphylococcus spp. (65.85%), Pseudomonas spp. (12.19%) and Enterobacteria species (19.51%). 53.66% of the isolates were considered multiresistant. Antimicrobial agents considered most resistant in the strains studied were penicillin (75.00%), tetracyclin (50.00%), amoxicillin (48.78%), trimethoprimsulfamethoxazole (46.15%), clindamycin and rifampicin (43.24%). 11 strains were phenotypically characterized as MRS, 4 genotypically as MRS, 2 as MLSB-MRS and 2 as gram negative ESBL-producing. © 2015 Ricardo Antonio Pilegi Sfaciotte, Jéssica Taina Bordin Bordin, Vanessa Kelly Capoia Vignoto, Patrícia Marques Munhoz, Adriana Aparecida Pinto, Maria José Baptista Barbosa, Rejane Machado Cardozo, Sílvia Cristina Osaki and Sheila Rezler Wosiacki.


Caleffi-Ferracioli K.R.,Health Science University | Caleffi-Ferracioli K.R.,State University of MaringaParana | Amaral R.C.R.,State University of MaringaParana | Demitto F.O.,State University of MaringaParana | And 8 more authors.
Tuberculosis | Year: 2016

Summary The aim of the present study was to (i) evaluate the in vitro action of rifampicin (RIF), ethambutol or isoniazid with efflux pumps inhibitors (EPIs) in Mycobacterium tuberculosis (Mtb) H37Rv and (ii) evaluate the morphological and efflux pumps (EPs) transcriptional changes by the action of rifampicin + verapamil combination (RIF + VP). The minimal inhibitory concentration and synergic effect of drug combinations were determined by Resazurin Microtiter Plate Assay and Resazurin Drugs Combination Microtiter Assay, respectively. VP showed greater capacity of ethidium bromide accumulation and RIF + VP had the lower fractional inhibitory concentration index. The RIF + VP exerted a similar reduction of viable cell counts to RIF by time-kill curve, but decreases in the expression of EPs genes were observed by Real time PCR at 72 h of RIF + VP exposure. Accumulative morphological changes (wrinkled and rounding) caused by each drug were observed by scanning electron microscopy after RIF + VP exposure. The downexpression of EPs related genes exposed to RIF + VP, suggest an effective inhibitory activity of VP in Mtb H37Rv. The role of EPs and the use of EPIs open up a powerful approach and the RIF + VP combination should be studied in Mtb more thoroughly. © 2016 Elsevier Ltd. All rights reserved.


Spironello R.A.,State University of MaringaParana | Botura-Amado C.,State University of MaringaParana | Balensiefer T.K.,State University of MaringaParana | de Barros Carvalho M.D.,State University of MaringaParana | And 2 more authors.
Tropical Journal of Pharmaceutical Research | Year: 2015

This is a report on a case of neonatal sepsis and clinical management with multiple antibiotic therapy in a neonatal intensive care unit (NICU) in Brazil. A preterm baby boy was born by caesarean section at 34 weeks and two days of gestation from an oligodramnious pregnancy with intrauterine growth restriction. After respiratory failure detection, the baby was intubated and placed on mechanical ventilation for respiratory failure and was shifted to NICU. Ampicillin and gentamicin were instituted empirically. Enterobacter spp-induced severe sepsis was diagnosed. Included in the antibiotic therapy were oxacycline, amikacin, cefepime, tazocin, meropen and teicoplanin. After sixty days in NICU, clinical and laboratory parameters were normalized and the baby recovered. The empirical long-term antibiotic treatment and the use of broad spectrum antibiotics, as observed in the present case, should be carefully considered in newborns admitted to NICU. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.


Alczuk S.S.D.,State University of MaringaParana | Bonfim-Mendonca P.S.,State University of MaringaParana | Rocha-Brischiliari S.C.,State University of MaringaParana | Shinobu-Mesquita C.S.,State University of MaringaParana | And 7 more authors.
Revista do Instituto de Medicina Tropical de Sao Paulo | Year: 2015

Vulvovaginal candidiasis (VVC) in HIV-infected women contributed to the impairment of their quality of life. The aim of this study was to evaluate the effect of highly active antiretroviral therapy (HAART) use on the vaginal Candida spp. isolation in HIV-infected compared to HIV-uninfected women. This cross-sectional study included 178 HIV-infected (HIV group) and 200 HIV-uninfected women (control) that were studied at the Specialized Assistance Service (SAE) for sexually transmitted diseases (STD)/AIDS of the city of Maringá, Brazil, from April 1 to October 30,2011. The yeasts were isolated and identified by phenotypic and molecular methods. The in vitro antifungal susceptibility to fluconazole, itraconazole, nystatin and amphotericin B was tested by the reference microdilution method. Higher frequencies of total vaginal Candida spp. isolation were found in the HIV-infected group than in the control group. However, both groups showed a similar frequency of colonization and VVC. Although C. albicans was the most frequent and sensitive to azolics and polyenes in both HIV-infected and uninfected women, the emerging resistance of C. glabrata to amphotericin B in the HIV-infected women was observed. Although higher frequency of vaginal Candida spp. isolation had been observed in the HIV-infected than in HIV-uninfected women, colonization and VVC showed similar frequency in both groups, indicating that HAART appears to protect against vaginal colonization and VVC. © 2015, Instituto de Medicina Tropical de Sao Paulo. All Rights Reserved.


Seidel A.C.,State University of MaringaParana | Cavalari P.,State University of MaringaParana | Rossi R.M.,State University of MaringaParana | Miranda F.,Federal University of São Paulo
Annals of Vascular Surgery | Year: 2016

Background The lack of the great saphenous vein (GSV) in its compartment is rarely mentioned in literature, although it happens in individuals with or without insufficiency of it. With the help of the B-mode ultrasound examination this vein can be easily identified. The aim of this study was to propose a classification for the findings. Methods Prospective study carried out for a period of 6 months in a sample of 2,665 lower limbs with ages ranging from 17 to 85, being that 1,286 patients are female. These patients underwent B-mode ultrasound examination as recommended by the literature. This evaluation determined whether there was a GSV aplasia by the analysis of its location in the saphenous compartment. Results After images were taken they were classified as: type I–aplasia only along the thigh, type II–aplasia only along the calf, type III–aplasia in the distal section of the thigh and proximal calf, type IV–vein in the saphenous compartment in the thigh and aplasia in the whole calf, type V–vein in the saphenous compartment only in a short segment in the proximal thigh, and type VI–vein with short segment in the saphenous compartment in the distal calf. From the total of 2,665 limbs, aplasia was found in 442 (16.6%). Conclusions These anatomic findings attain an important role in daily practice, influencing the surgical decision, particularly with the arrival of endovascular procedures, such as the use of laser and thermoablation. © 2016

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