Khan F.,The University of Asia Pacific |
Islam M.S.,University of Dhaka |
Roni M.A.,State University of Bangladesh |
Jalil R.-U.,University of Dhaka
Scientia Pharmaceutica | Year: 2012
The aim of this study was to prepare and characterize a self-emulsifying drug delivery system (SEDDS) with a high drug load of poorly water-soluble atorvastatin for the enhancement of dissolution and oral bioavailability. Solubility of atorvastatin in oil, surfactant, and cosurfactant was determined. Pseudo-ternary phase diagrams were constructed by the aqueous titration method, and formulations were developed based on the optimum excipient combinations. A high drug load (10% w/w) was achieved with a combination of oleic acid, Tween 80, and polyethylene glycol 400, ensuring the maximum dissolution property (in the case of SES6). Effects of lipids and surfactants on physical properties of SEDDS such as in vitro emulsification efficiency in terms of self-emulsification time, emulsion droplet size, and percent transmittance were measured. Multiple regression analysis revealed that a higher amount of surfactants significantly increased dissolution of ATV while decreasing emulsion droplet size and emulsification time. About a four-fold increase in dissolution was achieved by SEDDS compared to pure ATV powder. Overall, this study suggests that dissolution and oral bioavailability of ATV could be improved by SEDDS technology. © Khan et al.
Rahman K.F.,State University of Bangladesh
Clinical Governance | Year: 2014
Purpose - The purpose of this paper is to review different perspectives of health and illness keeping the human being at the center.Design/methodology/approach - This paper has been written based on the review of secondary literature.Findings - The analysis indicates that the appropriate perspective to use depends on normative considerations and the particular policy context to which it will be applied. Research limitations/implications - The main limitation was time and resources, which were overcome by meticulous analysis of secondary data and also through comparison of the results by achieving triangulation. Practical implications - The paper analyzed different perspectives of health and illness in order to increase the insight to the phenomena of health and illness; and also to reach a decision whether consensus among the perspectives is possible or otherwise.Originality/value - After analyzing the existing perspectives of health and illness, this paper proposes that an appropriate approach is needed to reconcile different perspectives for sustainable use. © Emerald Group Publishing Limited.
Laboni F.R.,World University of Bangladesh |
Akhter M.,State University of Bangladesh |
Batul U.K.,World University of Bangladesh
Pharmacologyonline | Year: 2013
The crude ethanolic extract of the stem of Tinospora cordifolia (T. cordifolia) (Family: Menispermaceae) was evaluated for its possible antinociceptive and antioxidant properties growing in the most of the part of Bangladesh. The ethanolic extract of stems of T. cordifolia exhibited statistically significant (p>0.001) writhing inhibition in acetic acid induced writhing model in white albino mice (Swiss-webstar strain). The crude extract produced 40 % inhibition of writhing at the dose of 500 mg/kg body weight while the standard drug diclofenac inhibition was found to 45.22% at a dose of 25 mg/kg body weight. The antioxidant property of ethanolic extract of T. cordifolia was assessed by DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical scavenging activity. In DPPH scavenging assay the IC50 value was found to be (83.52 μg/ml) which was comparable to the standard ascorbic acid (98.16 μg/ml). Phytochemical nature (group determination of plant constituent) and selected phytochemical analysis of the ethanolic extract of the stems of T. cordifolia indicated the presence of steroid, reducing sugars, tannin & saponin types of compounds. The obtained results provide a support for the use of this plant in traditional medicine and its further investigation.
Nahar N.,State University of Bangladesh |
Rashid R.B.,State University of Bangladesh
Dhaka University Journal of Pharmaceutical Sciences | Year: 2017
Vibrio cholerae has long been reported as an important cause of death in developing countries. The study detected the virulence and antibiotic resistance gene of eight V. cholerae isolates through in silico tools. Cholera toxins, ctxA and ctxB were found in six isolates (75%). Seventy-five percent isolates were also found to be positive for zonula occludens toxin, zot which is known to increase the permeability by altering the tight junction of the small intestine. Accessory cholera enterotoxin ace, responsible for fluid accumulation, was detected in four V. cholerae strains. Seven isolates (87.5%) were positive for toxin-coregulated pilus, tcp which helps the bacteria to adhere to gut mucosa. Both ompW and toxR genes were found in 87.5% of the isolates. Twenty-five percent isolates harboured strA, strB, sulII, dfrA1, floR genes and SXT element demonstrating that they were multidrug-resistant (MDG) bacterium. One isolate was found to be positive for tetA gene while no erythromycin resistance gene, ermA and ermB was found. Virulence genes were found in all genotypes indicating that their distribution was not genotypeoriented while genotype 2 harboured no antibiotic resistance genes. This data helps to predict virulence genes associated with cholera and also demonstrates the presence of antibiotic resistance genes. Bacteria acquired the antibiotic resistance gene through natural process which cannot be stopped. So by analyzing the resistance pattern we can choose appropriate antibiotics. In silico study helps us to predict the antibiotic resistant genotypes and can easily identify antibiotic resistant strains which help us to treat cholera infections and reduce the morbidity and mortality rate of the infected individuals. © 2017, University of Dhaka. All rights reserved.
Nahar N.,State University of Bangladesh |
Rashid R.B.,State University of Bangladesh
Dhaka University Journal of Pharmaceutical Sciences | Year: 2017
A total of twelve isolates were screened for virulence and antibiotic resistance genes associated with Klebsiella pneumoniae infections. Virulence and antibiotic resistance genes were detected by in silico PCR amplification. Iron uptake protein entB was detected in 66.67% (n=8) of the isolates while no isolate was found to harbour chelating agent irp2. Iron uptake system kfu, involved in purulent tissue infections and capsule formation, was identified in 25% (n=3) of the isolates. Regulator of mucoid phenotype A, rmpA was not found in any of the isolates. The wabG gene, responsible for urinary tract infections was found in seven K. pneumoniae strains. Five uge positive strains might play role in the pathogenicity of K. pneumoniae infections. About 83.33% of the isolates were positive for type 1 fimbriae fimH1 while no type 3 fimbriae mrkD gene was found. Complement reaction blocked by plasmid traT gene was not observed in Klebsiella species while eight isolates harboured outer membrane lipoprotein, ycfM which protects Klebsiella species from antibiotics. Antibiotic resistance genes blaTEM and blaSHV were detected in 33.33% (n=4) and 66.67% (n=8) of the isolates while 25% isolates carried both blaTEM and blaSHV genes. Genotype 1 carried fimH1 and ycfM genes while all the virulence genes studied were present in genotype 2 and 3. The blaSHV gene was detected in all the genotypes while blaTEM gene was found in only genotype 1 and 3. The findings of this study would be helpful to predict virulence gene associated with Klebsiella infections. This data also helps us to choose antibiotics for treating Klebsiella infections. By assessing the genotypic distribution of antibiotic resistance gene, correct antibiotic can be used to treat the infection. This could help reduce emergence of antibiotic resistance since it is known that incorrect choice of antibiotics contributes to antibiotic resistance. © 2017, University of Dhaka. All rights reserved.
Tasmin S.,University of Tokyo |
Furusawa H.,University of Tokyo |
Ahmad S.A.,Bangladesh Institute of Health science |
Faruquee M.H.,State University of Bangladesh |
Watanabe C.,University of Tokyo
Environmental Research | Year: 2015
Background and objective: Lead has long been recognized as a harmful environmental pollutant. People in developing countries like Bangladesh still have a higher risk of lead exposure. Previous research has suggested that the delta-aminolevulinic acid dehydratase (ALAD) genotype can modify lead toxicity and individual susceptibility. As children are more susceptible to lead-induced toxicity, this study investigated whether the ALAD genotype influenced urinary excretion of delta-aminolevulinic acid (U-ALA) among children exposed to environmental lead in Bangladesh. Methods: Subjects were elementary schoolchildren from a semi-urban industrialized area in Bangladesh. A total of 222 children were studied. Blood and urine were collected to determine ALAD genotypes, blood lead levels and urinary aminolevulinic acid (U-ALA). Results: The mean BPb level was 9.7. μg/dl for the study children. BPb was significantly positively correlated with hemoglobin (p<0.01). In total, allele frequency for ALAD 1 and 2 was 0.83 and 0.17 respectively. The mean U-ALA concentration was lower in ALAD1-2/2-2 carriers than ALAD1-1 carriers for boys (p=0.001). But for girls, U-ALA did not differ significantly by genotype (p=0.26). When U-ALA was compared by genotype at the same exposure level in a multiple linear regression analysis, boys who were ALAD1-2/2-2 carriers still had a lower level of U-ALA compared to ALAD1-1carriers. Conclusion: This study provides information about the influence of ALAD polymorphism and its association with U-ALA in Bangladeshi children. Our results indicate that the ALAD1-2/2-2 genotype may have a protective effect in terms of U-ALA for environmentally lead exposed boys. © 2014 Elsevier Inc.
Sultana F.,State University of Bangladesh |
Manirujjaman,State University of Bangladesh |
Imran-Ul-Haque,Healthcare Pharmaceuticals Ltd |
Arafat M.,The University of Asia Pacific |
Sharmin S.,University of Dhaka
Journal of Applied Pharmaceutical Science | Year: 2013
Nanogels based materials have high drug loading capacity, biocompatibility, and biodegradability which are the key points to design a drug delivery system effectively. The pursuit of this review article is to concisely describe the recent development of nanogel drug delivery system in terms of drug loading and swelling of drug from nanogels. Furthermore, biomedical application and current clinical trial studies of nanogel are summarized briefly. Here, different types of nanogels along with the synthetic procedure and mechanism of drug release from nanogel carrier are mainly focused. An intensive study of clinical trial in future will confirm nanogel as a suitable carrier for drug delivery. © 2013 Farhana Sultana et al.
Al Amin Sikder M.,State University of Bangladesh |
Siddique A.B.,State University of Bangladesh |
Nawshad Hossian A.K.M.,State University of Bangladesh |
Kowser Miah M.,State University of Bangladesh |
And 2 more authors.
Journal of Pharmacy and Nutrition Sciences | Year: 2011
Four Bangladeshi medicinal plants Sansevieria trifasciata, Justica gendarussa, Hydnocarpus kurzii and Mesua nagassarium have been investigated for their in vitro thrombolytic activity. The clot lysis activity was assessed by addition of the test material to the pre-clotted blood and incubation for 90 min. at 37°C and was expressed as % lysis of clot. Each of the plant was extracted with methanol at room temperature and the concentrated methanolic extract was fractionated by the modified Kupchan partitioning method to provide pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions. Among the four plants the aqueous soluble fraction of M. nagassarium, carbon tetrachloride soluble fraction of H. Kurzii , aqueous soluble fraction of methanolic extract of S. trifasciata exhibited highest thrombolytic activity with clot lysis value of 50.86%, 47.50%, and 47.10% respectively. However, the pet ether and carbon tetrachloride soluble fraction of methanolic extract of J. gendarussa demonstrated significant thrombolytic activity as evident from 45.93% and 45.47% lysis of clot, respectively. Standard streptokinase was used as positive control which exhibited 61.50% lysis of clot while the negative control water revealed 2.56% lysis of clot.
Maleque M.,State University of Bangladesh |
Hasan M.R.,University of Dhaka |
Hossen F.,University of Dhaka |
Safi S.,University of Dhaka
Journal of Pharmaceutical Analysis | Year: 2012
A rapid, specific and economic UV spectrophotometric method has been developed using a solvent composed of water:methanol:acetonitrile (9:0.5:0.5) to determine the levofloxacin content in bulk and pharmaceutical dosage formulations. At a pre-determined λmax of 292 nm, it was proved linear in the range of 1.0-12.0 μg/mL, and exhibited good correlation coefficient (R2=0.9998) and excellent mean recovery (99.00-100.07%). This method was successfully applied to the determination of levofloxacin content in five marketed brands from Bangladesh and the results were in good agreement with the label claims. The method was validated statistically and by recovery studies for linearity, precision, repeatability, and reproducibility. The obtained results proved that the method can be employed for the routine analysis of levofloxacin in bulks as well as in the commercial formulations. © 2012 Xi'an Jiaotong University.
Mahjabin T.,State University of Bangladesh |
Hossain S.M.,University of Dhaka |
Haque M.S.,Jaxara IT Ltd
Proceeding of the 15th International Conference on Computer and Information Technology, ICCIT 2012 | Year: 2012
Information hiding is a historical but on demand fascinating research area. Now, in today's world, the availability of information has become so easy that the security and insecurity of information goes side by side. In order to provide security of information a science called, Steganography has emerged. Steganography conceals the existence of information into images to formulate a secure communication. In this paper, a Steganographic method based on Pixel Value Differencing (PVD) and LSB Substitution method is proposed. To meet the increasing demand for privacy and secrecy the method focused mainly on making it a robust, secured technique of information hiding. An efficient and dynamic embedding algorithm is proposed in this paper that not only hides the secret data with an imperceptible visual quality and increased capacity but also make secret code breaking a good annoyance for the attacker. This method also represents an extraction algorithm that effectively extracts the entire secret message without any loss of a single data. Finally, experimental results show that, the proposed method achieves an increased embedding capacity and lower image degradation with improved security as compared to LSB substitution method and some recent existing methods of data hiding. © 2012 IEEE.