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Mezaka I.,State Priekuli Plant Breeding Institute | Bleidere M.,State Stende Cereal Breeding Institute | Legzdina L.,State Priekuli Plant Breeding Institute | Rostoks N.,University of Latvia
Zemdirbyste | Year: 2011

ß-glucan content in barley grain is an important component in determination of seed quality and end use. While high ß-glucan content is considered beneficial for human food, it is undesirable in malting barley and feed. Association of ß-glucan content in grain of 89 barley accessions of Latvian origin including 22 hulless accessions with 1273 high quality genome wide Single Nucleotide Polymorphism markers was tested. P-values were calculated using mixed linear model combining Q matrix and K matrix by computer program Tassel. P-values were adjusted using Bonferroni correction. Significant associations were identified for three markers 1_0673 (114.58 cM), 2_0880 (116.68 cM) and 1_1445 (116.68 cM) on chromosome 7H bin 7. Recessive mutation in the NUD locus also located on chromosome 7H bin 7, which determines hulless barley phenotype and has been previously suspected to affect ß-glucan content, was also genotyped and included in the association analysis.Asignificant marker - trait association was found identifying NUD gene as a major source of variation for ß-glucan content in our association mapping panel.

Nakurte I.,University of Latvia | Klavins K.,University of Latvia | Kirhnere I.,State Priekuli Plant Breeding Institute | Namniece J.,University of Latvia | And 7 more authors.
Journal of Cereal Science | Year: 2012

Lunasin is a novel, cancer-preventive, anti-inflammatory and cholesterol-reducing peptide that was originally isolated from soy and later from barley, wheat and rye. We report the first discovery of lunasin in triticale (X Triticosecale Wittmack). Moreover, we report first data of lunasin content in winter rye and wheat genotypes grown in Northern Europe. These data are novel as previously published data on finding of lunasin in cereals were obtained in genotypes grown in Korea. Lunasin content was uncovered using a previously published procedure for isolation from cereals and identified by LC-MS/MS assay. We found that triticale was the most lunasin-rich cereal, with the tested genotypes displaying the following trend in lunasin content: genotype 0002-26 > Dinaro > DSGU 10/94 > 0213-22 > 0317-14 > 0006-31. The greatest lunasin content was 6.46 mg/g in the grain of triticale genotype 0002-26. In comparison, the highest lunasin content in rye variety Dankovske Diament was 1.5 mg/g, and the highest lunasin content in the winter wheat variety Fredis was 0.23 mg/g. We conclude that triticale can play a significant role as functional food, with great potential for the use of triticale products in human and animal diets. © 2012 Elsevier Ltd.

Bleidere M.,State Stende Cereal Breeding Institute
Research for Rural Development | Year: 2010

The objectives of the investigation were to determine the transgressive lines of spring barley (Hordeum vulgare L.) for grain quality traits (1000 kernel weight, and content of crude protein, starch and β-glucans) in the six cross combinations between covered and hulless barley genotypes. The trial was carried out at the State Stende Cereal Breeding Institute during 2007 and 2008. Lines representing either positive or negative transgression were found in each cross combination if compared covered and hulless lines with their parent varieties. These lines during the two years of investigation exceeded both parents in positive or negative direction by one standard deviation according to any of the four analyzed grain quality traits. Seven positive transgressive lines were observed regarding 1000 kernel weight, and two transgressive genotypes regarding crude protein content within the population of covered barley lines. No line with positive transgression regarding starch and β-glucan content was found within the covered barley population. 27 hulless lines showed positive transgression regarding any of the four analyzed grain quality traits. The results show that it is possible to increase grain quality of spring barley grain by implementing selection practices.

Sterna V.,State Stende Cereal Breeding Institute | Zute S.,State Stende Cereal Breeding Institute | Jakobsone I.,University of Latvia
Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences | Year: 2015

Cereals, including barley, have been recognised as functional foods that provide beneficial effect on the health of the consumer and decrease the risk of various diseases. The aim of investigation was to determine the grain composition of barley varieties and perspective breeding lines bred in Latvia and to evaluate its functional ingredients. The results of analysis showed that protein content among varieties ranged from 106.6-146.8 g·kg-1, total dietary fibre 187.4-208.2 g·kg-1, β-glucans 42.8 g-49.4 g·kg-1, and amount of α-tocopherol 6.03-8.93 mg·kg-1. The sum of essential amino acids in barley grain samples was from 32.90 g·kg-1 to 38.71 g·kg-1. All varieties of hulled and hulless barley grain were found to be sources of protein with high biological value. Comparison of barley varieties bred in Latvia suggests that variety 'Kornelija' outperforms others in protein, dietary fibre and micronutrient content. © by Vita Šterna 2015.

Tamm Y.,Estonian Crop Research Institute | Tamm Y.,State Stende Cereal Breeding Institute | Jansone I.,State Stende Cereal Breeding Institute | Zute S.,State Stende Cereal Breeding Institute | Jakobsone I.,University of Latvia
Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences | Year: 2015

The aim of this study was to evaluate the genetic and environmental variation of yield and food quality characteristics (protein, starch, β-glucan and husk content, test weight and thousand grain weight) of covered and hulless barley genotypes in organic management conditions. Two different field experiments were carried out. In the first experiment, hulless barley genotypes 'Kornelija', 'Irbe', '1165', '1185' and the covered varieties 'Ansis' and 'Maali' were studied in two locations in Latvia and in one location in Estonia during 2014. 'Ansis', 'Kornelija', '1165' and '1185' were included in the second experiment carried out at Stende during three sequential years (2011-2013). The effect of genotype, environment (location and year) and co-effect of these factors contributed significantly to the variation in yield and grain quality traits of the barley genotypes. As a result of experimental treatments grain yield and quality traits varied by all tested factors. There were considerable differences in impact of studied sources of variation. Grain yield was influenced more by environmental factors than by genotype. Impact of genotype was highest for husk content and test weight. Integral evaluation method was carried out for comparison of barley genotypes to identify the best ones by the lowest deviation from the desired value. The covered barley variety 'Maali' showed good adaptation to different environmental conditions, having good yield, β-glucan content and high thousand grain weight. The line '1165' and variety 'Kornelija' showed best results among hulless barley varieties. © 2015 by Ylle Tamm.

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