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Kirillova N.E.,State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms | Levitin E.I.,State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms | Yudina T.G.,Moscow State University | Zalunin I.A.,State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms | Chestukhina G.G.,State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms
Applied Biochemistry and Microbiology | Year: 2010

An expression system for an effective production of recombinant protein Cry9A in bacillary cell has been suggested in the study. The proteins' immunological properties, ability to proteolysis, and biological activity were identical to natural protein. The ability of recombinant Cry9A to form crystal bodies in sporulating cells of Bacillus thuringiensis has been shown. Thus, the first evidences of the fact that Cry-proteins which in natural strains form the crystal bodies together with other endotoxins are able to independently form the crystals has been received. The introduced system including vector replicative carriers, expression cassettes, and a protocol of obtaining and cultivation of strain-producer allows simple manipulations with the gene of delta-endotoxin of Cry9A in gene-engineering experiments. © 2010 Pleiades Publishing, Ltd. Source

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