Entity

Time filter

Source Type


Javmen A.,State Scientific Research Institute Center for Innovative Medicine | Mauricas M.,State Scientific Research Institute Center for Innovative Medicine | Nemeikaite-Ceniene A.,State Scientific Research Institute Center for Innovative Medicine | Grigiskis S.,JSC Biocentras
International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings | Year: 2014

Nowadays non-cellulosic, β-glucans from the different source are intensively investigated due to the fact that these biological polymers are recognized as potent immunological stimulators for the mammal's immune system. Dendritic cells are the most important antigen presenting cells for activating naive T cells. The research goals of the recent investigation were to determine how the different molecular weight Saccharomyces cerevisiae β- glucan preparations affect such properties of the murine DC as phagocytosis, proliferation and cytokine synthesis in vitro. © 2015 IEEE. Source


Javmen A.,JSC Biocentras | Javmen A.,State Scientific Research Institute Center for Innovative Medicine | Grigiskis S.,JSC Biocentras | Rudenkov M.,Vilnius Gediminas Technical University | Mauricas M.,State Scientific Research Institute Center for Innovative Medicine
Protein Journal | Year: 2013

A β-1,3-endoglucanase produced by Streptomyces rutgersensis was purified to a homogeneity by the fractional precipitation with ammonium sulfate, ion exchange chromatography on Q-Sepharose and hydrophobic chromatography on Butyl Sepharose. A typical procedure provided 11.74-fold purification with 12.53 % yield. SDS-PAGE of the purified protein showed one protein band. The exact molecular mass of the enzyme obtained by mass spectrometry was 41.25 kDa; the isoelectric point was between pH 4.2-4.4. The optimal β-glucanase catalytic activity was at pH 7 and 50 C. An enzyme was only active toward glucose polymers containing β-1,3 linkages and hydrolyzed Saccharomyces cerevisiae cell wall β-glucan in an endo-like way: reaction products were different molecular size β-glucans, which were larger than glucose. © 2013 Springer Science+Business Media New York. Source


Javmen A.,State Scientific Research Institute Center for Innovative Medicine | Nemeikaite-Ceniene A.,State Scientific Research Institute Center for Innovative Medicine | Bratchikov M.,State Scientific Research Institute Center for Innovative Medicine | Bratchikov M.,Vilnius University | And 6 more authors.
In Vivo | Year: 2015

Aim: β-Glucan is one of the most abundant polymers in nature and has been established as an immunomodulator. This compound has notable physiological effects on mammalian immune systems, including anti-tumor and anti-infective activities and can activate the immune response. It is considered that the immune-stimulating activities of β-glucan can depend on physicochemical parameters, such as molecular size. Saccharomyces cerevisiae, also known as baker's yeast, is a frequently used source of β-glucan. The aim of the experiments was to investigate how different Saccharomyces cerevisiae β-glucan preparations with different molecular size affect interferon-gamma (IFN-γ) production in BALB/c mice. Materials and Methods: In vivo and in vitro BALB/c mouse models were used for the investigations. Different β-glucan preparations were orally administrated in the in vivo experiments. IFN-γ production in BALB/c mice was analyzed by enzyme-linked immunosorbent assay and measuring interferon-γ RNA concentration. Results: The results showed that orally-administered β-glucan from S. cerevisiae enhanced IFN-γ production in BALB/c mice in the in vivo model, but not by mouse leukocytes in vitro. Moreover, water-soluble β-glucan enhanced IFN-γ production more effectively than did particulate β-glucan. Conclusion: IFN-γ plays an important role in immunity against viral and bacterial infections. Our experiments have shown that β-glucan preparations enhance IFN-γ production in BALB/c mice and can be potentially used for immune system stimulation in mammals. Current results may be used to develop soluble β-glucan nutritional supplements. © 2015, International Institute of Anticancer Research. All rights reserved. Source


Javmen A.,JSC Biocentras | Javmen A.,State Scientific Research Institute Center for Innovative Medicine | Nemeikaite-Ceniene A.,State Scientific Research Institute Center for Innovative Medicine | Grigiskis S.,JSC Biocentras | And 4 more authors.
Turkish Journal of Biology | Year: 2015

β-Glucan is a natural polymer, which is widely studied due to its multiple immunomodulatory properties. In addition, recent findings indicate potent antitumor properties of β-glucan. Saccharomyces cerevisiae, baker’s yeast, is one of the commonly used sources of β-1,3-glucan. The aim of this work was to investigate S. cerevisiae β-glucan immunomodulatory activity against cancer cells. In our experiments, BALB/c mice were fed with insoluble whole β-glucan particles, and then their blood was collected for experiments. MH22a hepatoma cells were treated with the blood of mice fed with β-glucan, and tumor cell viability was investigated after the treatment. The obtained results demonstrated that leukocytes in vivo primed with whole glucan particles, in combination with soluble β-glucan, decreased MH-22a hepatoma cell viability in vitro. Our study has indicated that β-glucan obtained from S. cerevisiae potentially primes mouse whole blood leukocytes to induce cell death of mouse hepatoma cells. © 2015 TÜBİTAK Source

Discover hidden collaborations