Sklyarenko A.V.,State Research Institute of Genetics and Selection of Industrial Microorganisms GosNII genetika |
Berezina O.V.,State Research Institute of Genetics and Selection of Industrial Microorganisms GosNII genetika |
Satarova D.E.,State Research Institute of Genetics and Selection of Industrial Microorganisms GosNII genetika |
Fedorchuk V.V.,Moscow State University |
And 4 more authors.
Moscow University Chemistry Bulletin | Year: 2014
Recombinant, as well as native alpha-amino acid ester hydrolase from Xanthomonas rubrilineans VKPM B-9915 (XrAEH, EC 184.108.40.206), was tested for synthesis of amino-beta-lactam antibiotic cephalexin. It was shown that the recombinant enzyme r-XrAEH produced by Escherichia coli VKPM B-11246 is more efficient in comparison with the native enzyme wt-XrAEH prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915. When r-XrAEH was used as a biocatalyst, addition of ethylene glycol (33 vol %) to the reaction medium improved the yield from 70 to 95%. During synthesis of cephalexin under optimal conditions in the case of the native enzyme wt-XrAEH the cephalexin yield was 85%, in contrast to r-XrAEH where it was 95%. Furthermore, unlike native wt-XrAEH enzymes, preparations of recombinant r-XrAEH do not possess beta-lactamase side activity. © 2014 Allerton Press, Inc.