Time filter

Source Type

Sklyarenko A.V.,State Research Institute of Genetics and Selection of Industrial Microorganisms GosNII genetika | Berezina O.V.,State Research Institute of Genetics and Selection of Industrial Microorganisms GosNII genetika | Satarova D.E.,State Research Institute of Genetics and Selection of Industrial Microorganisms GosNII genetika | Fedorchuk V.V.,Moscow State University | And 4 more authors.
Moscow University Chemistry Bulletin | Year: 2014

Recombinant, as well as native alpha-amino acid ester hydrolase from Xanthomonas rubrilineans VKPM B-9915 (XrAEH, EC 3.1.1.43), was tested for synthesis of amino-beta-lactam antibiotic cephalexin. It was shown that the recombinant enzyme r-XrAEH produced by Escherichia coli VKPM B-11246 is more efficient in comparison with the native enzyme wt-XrAEH prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915. When r-XrAEH was used as a biocatalyst, addition of ethylene glycol (33 vol %) to the reaction medium improved the yield from 70 to 95%. During synthesis of cephalexin under optimal conditions in the case of the native enzyme wt-XrAEH the cephalexin yield was 85%, in contrast to r-XrAEH where it was 95%. Furthermore, unlike native wt-XrAEH enzymes, preparations of recombinant r-XrAEH do not possess beta-lactamase side activity. © 2014 Allerton Press, Inc.

Loading State Research Institute of Genetics and Selection of Industrial Microorganisms GosNII genetika collaborators
Loading State Research Institute of Genetics and Selection of Industrial Microorganisms GosNII genetika collaborators