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Kozyr A.V.,State Research Institute of Applied Microbiology and Biotechnology | Luneva N.M.,State Research Institute of Applied Microbiology and Biotechnology | Khlyntseva A.E.,State Research Institute of Applied Microbiology and Biotechnology | Shemyakin I.G.,State Research Institute of Applied Microbiology and Biotechnology | And 2 more authors.
Molecular Genetics, Microbiology and Virology | Year: 2013

New Delhi metallo-beta-lactamase (NDM-1) is a plasmid-borne enzyme conferring bacterial resistance to any known beta-lactam antibiotics. There is urgent need to develop efficient NDM-1 inhibitors and approaches for early diagnostics of NDM-1 that necessitates structural studies of the enzyme and analysis of the secretion pathways and localization of the protein. Recombinant full-length NDM-1 is produced in E. coli in active form and mostly accumulates in inclusion bodies. We developed a new system that uses antibiotic pressure to select E. coli producing increased quantities of the soluble NDM-1 and showed that the efficiency of production of the soluble NDM-1 depends on the level of chaperone activity in a bacterial cell. © 2013 Allerton Press, Inc.

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