Kazachenko K.Y.,The State Research Institute for Genetics and Selection of Industrial Microorganisms GosNIIgenetika |
Efremov B.D.,The State Research Institute for Genetics and Selection of Industrial Microorganisms GosNIIgenetika |
Kozlov D.G.,The State Research Institute for Genetics and Selection of Industrial Microorganisms GosNIIgenetika
Applied Biochemistry and Microbiology | Year: 2014
An analytical system for a quantitative assessment of contributions of pre- and pro-components of the yeast α-factor leader to key stages of somatropin secretion in Saccharomyces cerevisiae has been designed. Using this system, it was shown that: 1—the α-factor pro-peptide has contributed almost equally to the secretion at the Erv29p-dependent stage of vesicular transport and at the translocation stage; 2—of the two above stages, the Erv29p-dependent stage could only be optimized by using of two copies of the α-factor pro-peptide; 3—when the α-factor pre-peptide and the artificial pro-peptide with significantly greater hydrophobicity were compared it was found that somatropin secretion apparently depended on the translocation mode as higher secretion was achieved with hydrophobic artificial pre-peptide associated with the co-translational translocation. © 2014, Pleiades Publishing, Inc.