State Research Center Gos Genetika

Moscow, Russia

State Research Center Gos Genetika

Moscow, Russia
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Agapkina Y.V.,State Research Center Gos Genetika | Nikitin A.G.,State Research Center Gos Genetika | Brovkin A.N.,State Research Center Gos Genetika | Pushkov A.A.,State Research Center Gos Genetika | And 24 more authors.
Molecular Biology | Year: 2010

Associations of polymorphisms of genes FGB G(-455)A and PROC C(-1654)T with the frequency of poor outcomes in patients with the history of acute coronary syndrome (ACS) were studied in the Russian population. A total of 1145 patients admitted to cardiological hospitals of Moscow, St. Petersburg, Kazan, Chelyabinsk, Perm, Stavropol, and Rostov-on-Don with ischemic heart disease exacerbation were examined. The mean follow-up time was 1.14 ± 0.33 years, and the maximum follow-up time was 3.2 years. The risk of poor outcome did not depend on the carriership of genotypes of the polymorphic G(-455)A marker in the FGB gene. However, the PROC C(-1654)T polymorphism patients with ACS history and allele T of the PROC gene had a poor outcome more often than patients homozygous for allele C. The survival time to the endpoint for carriers of the TT and CT genotypes of the PROC gene was 2.19 ± 0.18 years vs. 2.46 ± 0.16 years for carriers of the CC genotype. On the base of these results it is suggested that hemostasis-related genes play an important role in early failures in patients with ACS history. © 2010 Pleiades Publishing, Ltd.


Blagodatskikh E.G.,State Research Center Gos Genetika | Nikitin A.G.,State Research Center Gos Genetika | Seregin Y.A.,State Research Center Gos Genetika | Blagodatskikh K.A.,State Research Center Gos Genetika | Nosikov V.V.,State Research Center Gos Genetika
Molecular Biology | Year: 2010

The applicability of real-time PCR amplification of the chromosome Y marker DYS14 for sex determination was studied. With this aim, real-time PCR of DYS14 (located within the TSPY1-encoding gene) was performed in plasma DNA specimens obtained from 30 men and 30 women. The PCR results showed that 30 specimens were of male and the other 30 were of female origin. All the results were confirmed by the tests for the SRY marker conventionally used in forensic examination. The detection limit for the DYS14-containing DNA region was established in dilution experiments and was equal to 6.7 pg of DNA (two copies of the genome), which corresponds to 6.7 ng of DNA (2000 copies of the genome) in 1 ml of blood. This level of sensitivity allows sex determination in specimens with small amounts of genetic material. The method can be used for noninvasive prenatal diagnostics of sex-linked congenital diseases and in forensic medical examination. © 2010 Pleiades Publishing, Ltd.


Shirokov D.A.,State Research Center Gos Genetika | Ryabichenko V.V.,State Research Center Gos Genetika | Akishina R.I.,State Research Center Gos Genetika | Ospelnikova T.P.,Gamaleya Institute of Epidemiology and Microbiology | And 3 more authors.
Molecular Biology | Year: 2011

We developed a technique for production of human interferon-α2a (IFN-α2a) and IFN-α2b lacking the N-terminal methionine. First of all, we constructed plasmids containing genes of hybrid IFN-α2 controlled by different promoters; the proximal part of the genes incorporated various sequences encoding the enteropeptidase cleavage site. As a result, four strains of Escherichia coli producing hybrid IFN-α2 were obtained. The protocol for IFN-α2 renaturation, cleavege of its N-terminal part, and chromatographic purification of the N-terminal methionine-free IFN-α2 was developed. © 2011 Pleiades Publishing, Ltd.

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