State Office for Agriculture

Rostock, Germany

State Office for Agriculture

Rostock, Germany
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Habedank F.,State Office for Agriculture | Abraham M.,Leibniz Institute for Baltic Sea Research | Tardel H.,State Office for Agriculture | Feldhusen F.,State Office for Agriculture | Schulz-Bull D.E.,Leibniz Institute for Baltic Sea Research
International Journal of Environmental Analytical Chemistry | Year: 2017

A sensitive enrichment method for the detection and quantification of 20 organophosphate pesticides in sea water and surface water was improved and validated. The compounds were extracted using ultrasound-assisted dispersive liquid–liquid microextraction and were analysed with an optimised Gas Chromatography - Tandem Quadrupole Mass Spectrometry (GC-MS/MS) method. Calibrations and validations were conducted for spiked local pond and Baltic Sea water. Recoveries of the organophosphate compounds from the different matrices ranged from about 25% to 70% and from 30% to 130% for pond and sea water, respectively. Obtained limit of detection (LoD) for both matrices was about 3 ng L−1 and the limit of quantification (k = 3) was 12 and 7 ng L−1 for pond and sea water, respectively. Valid extractability at the LoD level could be proven. © 2017 Informa UK Limited, trading as Taylor & Francis Group


Dannenberger D.,Leibniz Institute for Farm Animal Biology | Nuernberg G.,Leibniz Institute for Farm Animal Biology | Nuernberg K.,Leibniz Institute for Farm Animal Biology | Hagemann E.,State Office for Agriculture
Meat Science | Year: 2013

Samples of M. longissimus were collected from a total of 203 feral roe deer (n=118) and wild boar (n=85) in two regions of Mecklenburg-Western Pomerania (Germany). The muscle lipid saturated fatty acid proportions of roe deer and wild boar ranged between 33 and 49. g/100. g total fatty acids and 31 and 35. g/100. g total fatty acids, respectively. The total n-3 PUFA proportions in roe deer muscle varied between 8.0 and 14. g/100. g fatty acids, and in wild boar muscle between 2.6 and 6.0. g/100. g fatty acids. The major vitamin E homologue, α-tocopherol, was determined to be between 5.8 and 13.1. mg/kg in roe deer muscles. Lower levels between 1.2 and 4.7. mg/kg were measured in wild boar muscles. The iron and zinc concentrations in roe deer and wild boar muscle ranged from 26.3 to 33.9. mg/kg and from 17.0 to 21.7. mg/kg, and from 13.6 to 39.3. mg/kg and 18.1 to 31.9. mg/kg, respectively. © 2013 Elsevier Ltd.


Demasius W.,Leibniz Institute for Farm Animal Biology | Weikard R.,Leibniz Institute for Farm Animal Biology | Kromik A.,Leibniz Institute for Farm Animal Biology | Wolf C.,State Office for Agriculture | And 2 more authors.
Research in Veterinary Science | Year: 2014

Bovine neonatal pancytopenia (BNP) is a haemorrhagic disease of newborn calves elicited by colostrum from specific cows. Two studies have indicated that BNP-inducing colostrum might be associated with alloantibodies directed against MHC class I in response to vaccination with a distinct inactivated viral vaccine. However, the proportion of alloantibody-producing individuals by far exceeds the proportion of clinical BNP cases in the vaccinated population. This raises the question about the incidence of subclinical, unrecognised cases and also suggests further factors involved in BNP pathogenesis, e.g., genetic predisposition. Our results on neonatal calves from a closely monitored resource population confirmed the hypothesis of a genetic predisposition for clinical BNP and suggest that the predisposition is also involved in subclinical BNP-cases. No indication was obtained for a higher frequency of subclinical BNP-cases compared with clinical cases. Neither time point nor frequency of vaccination was a relevant factor for BNP in our resource population. © 2014 Elsevier Ltd.


Krappmann K.,Leibniz Institute for Farm Animal Biology | Weikard R.,Leibniz Institute for Farm Animal Biology | Gerst S.,State Office for Agriculture | Wolf C.,State Office for Agriculture | Kuhn C.,Leibniz Institute for Farm Animal Biology
Veterinary Journal | Year: 2011

Bovine neonatal pancytopenia (BNP) is a newly emerging disease in many European countries that causes haemorrhagic diathesis and mortality in neonatal calves. This study tested the hypothesis that genetic factors might be involved in BNP, since genetic defects resulting in coagulation disorders have been described in many species, including cattle. A familial pattern of occurrence of BNP cases was observed in an experimental population of cattle in Germany and BNP was diagnosed in nine calves on an experimental dairy herd from May 2007 to December 2009. All affected calves were descendents of a single F 1 sire in a specific F 2 resource population generated from Charolais and German Holstein bloodlines. Sequence analysis of the bovine coagulation factor XI (F11) gene as a functional candidate gene for BNP revealed an unusually high number of non-synonymous mutations within the gene compared to a whole genome mutation screen in cattle targetting random sequences. However, none of the mutations in the F11 gene were concordant with BNP status. Although these data and further pedigree analysis excluded a simple mode of inheritance of the BNP phenotype, there was a statistically significant (P= 0.0001) accumulation of BNP cases in the specific pedigree examined, suggesting that a genetic component is involved in the development of BNP. © 2010 Elsevier Ltd.


Mahecha L.,University of Antioquia | Dannenberger D.,Research Unit of Muscle Biology and Growth | Nuernberg K.,Research Unit of Muscle Biology and Growth | Nuernberg G.,Leibniz Institute for Farm Animal Biology | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

This study evaluated the influence of different n-3 and n-6 PUFA-enriched diets on the relationship between lipid peroxidation and antioxidant status by analyzing fatty acids (FA), lipid peroxidation, antioxidant capacity (AOC), antioxidant enzymes, trace elements, and fat-soluble vitamins in the longissimus muscle. Diet caused significant changes in muscle FA composition, leading to accumulation of beneficial n-3 FA. β-Carotene and catalase activity were significantly elevated in muscle of the n-3 PUFA-enriched diet group compared to the n-6 PUFA-enriched diet group. Lipid peroxidation was higher in muscle of the n-3 PUFA-enriched diet group after 15 min of reaction time. There was no significant effect of diet on AOC, but it increased with reaction time. The present results suggest that the antioxidant defense in muscle of the n-3 PUFA-enriched diet group could balance reactive substances under low oxidative conditions. However, the antioxidant capacity was not sufficient under abundant accumulation of reactive substances. © 2010 American Chemical Society.


PubMed | Martin Luther University of Halle Wittenberg, Equine Studies Group, State Office for Agriculture, University of Rostock and 2 more.
Type: Journal Article | Journal: PloS one | Year: 2017

The provision of NaCl, according to current recommendations, to horses in moderate work has been shown to induce immediate postprandial acidosis. The present study aimed to clarify whether this NaCl induced acidosis i) persists beyond the immediate postprandial period, and ii) is still present after a 2 week adaptation period. Six adult warmblood mares in moderate work received daily 1.00 kg hay per 100 kg body weight (bwt) only together with 0.64 kg unprocessed cereal grains/100 kg bwt.d as fed basis. Using a 3x3 Latin Square, either 0 (NaCl-0), 50 (NaCl-50) or 100 (NaCl-100) g NaCl/d were fed together with the concentrates in two equal doses for 3 weeks. During the final week, a mineral digestibility trial was undertaken. The middle sodium and chloride intake (NaCl-50) at least met the most common recommendations for moderate work. Morning (7:00 AM) urine and venous blood samples were collected on days 0, 1-4, 8, and 15, and analysed for pH, acid-base status, creatinine and electrolyte concentrations. Fractional electrolyte clearances (FC) were determined. Mean apparent sodium digestibility ranged between 60-62% whereas chloride digestibility was consistently above 94%. Supplementing 100 g but not 50 g of NaCl resulted in significant reduction of blood pH and base excess as well as urinary pH and urine acid excretion. Both 50 g and 100 g NaCl supplementation caused a significant reduction in base and net acid-base excretion, urine density and potassium concentration, but increased urine sodium concentration and the FC of sodium and chloride (P < 0.05). This suggests that a high proportion of the recommended salt doses is excreted renally. The above effects of NaCl supplementation persisted over the 2 week measurement period. Results suggest that feeding 100 g NaCl to moderately exercising horses results in mild metabolic acidosis, whereas feeding 50 g according to current recommendations resulted in compensated acidosis.


PubMed | Free University of Berlin, State Office for Agriculture and Leibniz Institute for Farm Animal Biology
Type: Journal Article | Journal: Research in veterinary science | Year: 2014

Bovine neonatal pancytopenia (BNP) is a haemorrhagic disease of newborn calves elicited by colostrum from specific cows. Two studies have indicated that BNP-inducing colostrum might be associated with alloantibodies directed against MHC class I in response to vaccination with a distinct inactivated viral vaccine. However, the proportion of alloantibody-producing individuals by far exceeds the proportion of clinical BNP cases in the vaccinated population. This raises the question about the incidence of subclinical, unrecognised cases and also suggests further factors involved in BNP pathogenesis, e.g., genetic predisposition. Our results on neonatal calves from a closely monitored resource population confirmed the hypothesis of a genetic predisposition for clinical BNP and suggest that the predisposition is also involved in subclinical BNP-cases. No indication was obtained for a higher frequency of subclinical BNP-cases compared with clinical cases. Neither time point nor frequency of vaccination was a relevant factor for BNP in our resource population.


Schnuch A.,University of Gottingen | Wolter J.,State Office for Agriculture | Geier J.,University of Gottingen | Uter W.,Friedrich - Alexander - University, Erlangen - Nuremberg
Contact Dermatitis | Year: 2011

Background. Nickel contact allergy is still frequent both in patch-tested patients and in the general population. Objectives. To explain this observation by relating clinical epidemiological data with recent chemical analyses of nickel release from costume jewellery. Methods. (i) The trend of nickel allergy was analysed using data registered between January 1994 and December 2009 in the Information Network of Departments of Dermatology. (ii) In 2008, different parts of items of costume jewellery purchased at random on the German market (n = 609) were analysed for nickel release according to EN 1811:1998 + A1:2008 in five official German laboratories of food and non-food investigation. Results. (i) Between 1994 and 2009, nickel allergy decreased in men (18-30 years) and in women (1-17 and 18-30 years); however, after 2000, there was no significant decrease in nickel allergy in the women aged 1-17 years. (ii) Of the post-assemblies, 28.0% exceeded the migration limit of ≥0.2 μg/cm 2 per week, and 5% released ≥26.8 μg/cm 2 per week. In articles with direct and prolonged contact with the skin, 12.8% of decorative parts and 17.1% of clasps exceeded the migration limit. If an adjustment factor was applied, according to the above norm, about half of the items otherwise rejected became acceptable. Conclusion. Exposure to nickel-containing products exceeding the (unnecessarily relaxed) permitted limit may explain why nickel contact allergy remains a problem. © 2011 John Wiley & Sons A/S.


Uter W.,Friedrich - Alexander - University, Erlangen - Nuremberg | Schmid M.,Friedrich - Alexander - University, Erlangen - Nuremberg | Schmidt O.,Institute for Consumer Products | Bock C.,Berlin Brandenburg State Laboratory | Wolter J.,State Office for Agriculture
Contact Dermatitis | Year: 2014

Background Contact sensitization to cobalt is common. Some industrial exposures have been identified, but cobalt allergy is also often diagnosed in 'non-occupational' patients. Exposure of consumers is largely unexplained. Objective To present the analytical results on cobalt release from earrings and piercing jewellery sampled in a German Federal surveillance scheme. Methods Two German state laboratories analysed cobalt release, after immersion in artificial sweat according to EN 1811, along with nickel release in 87 pieces of jewellery, which were mostly taken apart for separate examination of piercing posts (n = 139), clasps (n = 51), and/or decorative items (n = 52). The distribution of cobalt release was described by the use of Kaplan-Meier analysis, taking into account that the majority of measurements were left-censored, that is, below the limit of quantification. Results Thirty-eight of 87 earrings and piercing jewellery items had at least one part releasing cobalt. The median cobalt release was estimated as 0.013 μg/cm 2/week, and 75% of parts released up to 0.085 μg/cm 2/week. Release varied somewhat between the three parts, with, for example, 22.1% of posts releasing ≥ 0.2 μg/cm2/week. Conclusions Cobalt release from earrings and piercing jewellery, in particular from piercing posts, is considerable. Scientifically based exposure limits should be set, as in the case of nickel. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


PubMed | Erasmus Medical Center, Leibniz Institute for Zoo and Wildlife Research and State Office for Agriculture
Type: | Journal: Veterinary research | Year: 2015

Raptors may contract highly pathogenic avian influenza virus H5N1 by hunting or scavenging infected prey. However, natural H5N1 infection in raptors is rarely reported. Therefore, we tested raptors found dead during an H5N1 outbreak in wild waterbirds in Mecklenburg-Western Pomerania, Germany, in 2006 for H5N1-associated disease. We tested 624 raptors of nine species-common buzzard (385), Eurasian sparrowhawk (111), common kestrel (38), undetermined species of buzzard (36), white-tailed sea eagle (19), undetermined species of raptor (12), northern goshawk (10), peregrine falcon (6), red kite (3), rough-legged buzzard (3), and western marsh-harrier (1)-for H5N1 infection in tracheal or combined tracheal/cloacal swabs of all birds, and on major tissues of all white-tailed sea eagles. H5N1 infection was detected in two species: common buzzard (12 positive, 3.1%) and peregrine falcon (2 positive, 33.3%). In all necropsied birds (both peregrine falcons and the six freshest common buzzards), H5N1 was found most consistently and at the highest concentration in the brain, and the main H5N1-associated lesion was marked non-suppurative encephalitis. Other H5N1-associated lesions occurred in air sac, lung, oviduct, heart, pancreas, coelomic ganglion, and adrenal gland. Our results show that the main cause of death in H5N1-positive raptors was encephalitis. Our results imply that H5N1 outbreaks in wild waterbirds are more likely to lead to exposure to and mortality from H5N1 in raptors that hunt or scavenge medium-sized birds, such as common buzzards and peregrine falcons, than in raptors that hunt small birds and do not scavenge, such as Eurasian sparrowhawks and common kestrels.

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