State Key Laboratory of Tree Genetics and Breeding

Beijing, China

State Key Laboratory of Tree Genetics and Breeding

Beijing, China
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Liu C.,Chinese Academy of Forestry | Liu C.,State Key Laboratory of Tree Genetics and Breeding | Liu C.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | Dong Y.,Chinese Academy of Forestry | And 11 more authors.
Current Microbiology | Year: 2017

Acidobacteria is a new bacterial group, identified by molecular research, which is widely distributed and has specific ecological functions in forest soil. In this study, we investigated Acidobacteria response to N input, and the effects were related to N form and dose. The experimental design included two N forms (NH4 +-N and NO3 −-N) and five levels of N deposition (0, 20, 40, 60, 80 kg N ha−1) for 2 years. Research into the Acidobacteria community was conducted using 16Sr RNA gene-based high-throughput pyrosequencing methods. Acidobacteria OTUs and N had a negative relationship in 0–60 kg ha−1 year−1; however, at N doses beyond a certain size, nitrogen might promote an increase in Acidobacteria OTUs. The Acidobacteria relative abundance under NH4 +-N treatment was higher than under NO3 −-N treatment. Acidobacteria relative abundance decreased with increasing of NH4 +-N dose, but increased with increasing NO3 −-N dose. Overall, 13 different Acidobacteria subgroups were identified, with Gp1, Gp2, and Gp3 being dominant. Significant differences in Acidobacteria distribution were primarily caused by N input and pH value. The environmental factors of N were all negatively related to Acidobacteria distribution in low N dose treatments (0–20 kg ha−1 year−1), but were positively related in response to N dose treatments (40–80 kg ha−1 year−1). © 2017, Springer Science+Business Media New York.

Gao S.-H.,State Key Laboratory of Tree Genetics and Breeding | Tang Y.-X.,Hunan Academy of Forestry | Tang J.,Hunan Academy of Forestry | Tang X.-J.,State Key Laboratory of Tree Genetics and Breeding | And 4 more authors.
Forest Research | Year: 2017

Objective: To reveal the effects of clearcutting a poplar plantation at the floodplain of Yangtze River on evapotranspiration (ET) and water yield (WY). Method: Based on 3-years'consecutive observation data of water vapor flux from a poplar plantation on the floodplain of Yangtze River during the years before and after clearcutting by eddy covariance system, the variation of ET post-clearcutting was investigated by comparing the ETs in two periods (a whole year before and after clearcutting, respectively) with similar weather conditions. Then the variation of WY post-clearcutting was calculated by water balance. Result: (1) After clearcutting, both soil temperature and ground water level increased obviously while the soil water content decreased by about 0.03. (2) The ETs before and post clearcutting had the similar seasonal and diurnal dynamics; Nevertheless, the annual ET post-clearcutting was only 66.3% of that before clearcutting. (3) The WY ratio (WY/precipitation) increased from 0.53 to 0.62 after clearcutting. (4) The dryness index (potential ET/precipitation) was larger than 1 in July, August, and December, while less than 1 in other months in both before and post clearcutting period. Conclusion: Clearcutting a poplar plantation on the floodplain of Yangtze River leads to a decrease of ET and an increase of WY ratio. As a result, it is less likely to intensify regional drought in summer, but the capacity of the floodplain for flood peak clipping during flooding would also decline. © 2017, Chinese Academy of Forestry. All right reserved.

Xue L.,Chinese Academy of Forestry | Xue L.,State Key Laboratory of Tree Genetics and Breeding | Liu J.,Chinese Academy of Forestry | Liu J.,State Key Laboratory of Tree Genetics and Breeding | And 11 more authors.
Clean - Soil, Air, Water | Year: 2014

A field survey of native herbaceous plants and associated soil in an antimony mining area of Xikuangshan, Hunan Province, China was conducted to identify species that accumulate heavy metals in their tissues. The results indicate that the soils in the mining area were contaminated mainly by As and Sb, at concentrations of 423.77-526.57 and 228.37-445.20mg/kg, respectively. Of the herbaceous plants, Miscanthus sinensis and Imperata cylindrica exhibited Hg and Cd phytoextraction, with bioconcentration factors (BCFs) and translocation factors (TFs) greater than 1. Moreover, Phytolacca americana could be used for the phytostabilization of Pb and Cd (BCF=1.06 and 7.66, respectively), and Cynodon dactylon had considerable potential for As and Sb stabilization (BCF=2.02 and 6.62, respectively). Boehmeria was capable of accumulating Sb and As in its shoots (TF=3.12 and 4.86, respectively). Additionally, the concentration of Sb in the roots of C. dactylon reached 2209.3mg/kg, which is the highest Sb concentration reported in a plant species to date. Our data suggest that native herbaceous plants growing in metal-contaminated sites have phytoremediation potential. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Zhao H.,State Key Laboratory of Tree Genetics and Breeding | Zhao H.,Chinese Academy of Forestry | Zheng Y.-Q.,State Key Laboratory of Tree Genetics and Breeding | Li B.,State Key Laboratory of Tree Genetics and Breeding | And 5 more authors.
Forest Research | Year: 2014

In order to explore the genetic variation among Pinus bungeana populations, their genetic diversity and differentiation level were studied in 21 natural populations by EST-SSR markers. 14 polymorphic loci were detected in 663 individuals among 21 natural populations using 7 pairs of EST-SSR primes. The effective number of alleles (Ne), Shannon's information index (I), observed heterozygosity (Ho), expected heterozygosity (He) and Nei's expected heterozygosity (Nei's) were 1.156 5-1.601 9, 0.133 5-0.492 5, 0.138 4-0.397 3, 0.086-0.342 8, and 0.084 6-0.337 4 respectively. The results of genetic differentiation analysis showed that the average Fst and Nm were 0.215 2 and 0.911 9 respectively, the gene flow was less and the genetic differentiation was high among populations. Central regions of genetic diversity are mainly in west and south of the distribution and the genetic diversity in distribution changed regularly from west to east and from south to north.

Zheng S.-X.,State Key Laboratory of Tree Genetics and Breeding | Zheng S.-X.,Chinese Academy of Forestry | Zhang J.-G.,State Key Laboratory of Tree Genetics and Breeding | He C.-Y.,State Key Laboratory of Tree Genetics and Breeding | And 4 more authors.
Forest Research | Year: 2015

Along the Erqis River, there are various natural Populus forests, which include Section Leuce in Populus, such as Populus alba, Populus canescens and Populus tremula. Clonal diversity and structure were studied using nuclear microsatellite markers. The results showed that clonal reproduction was the primary reproduction strategy of these Populus populations. The populations of P. alba and P. tremula had an abundant clonal diversity and the mean Simpson's index were 0.983 and 0.987, respectively. Compared with the P. tremula, Populus alba had lower genetic diversity and their Shannon' index were 1.068 9 and 0.324 9, Nei' index were 0.505 6 and 0.211 2, respectively. The genetic consistency was high among populations, and variation range were 0.778 1 0.954 4 in P. tremula, 0.975 1 0.994 6 in Populus alba. It suggested they had long-distance gene flow and prevented genetic differentiation among P. alba and P. tremula population. In addition, genetic diversity indicated that the genetic variation occurred within natural populations, which were 95% in P. alba, and 89.98% in P. tremula. ©, 2015, Chinese Academy of Forestry. All right reserved.

Xie F.,East Carolina University | Burklew C.E.,East Carolina University | Yang Y.,Chinese Academy of Forestry | Yang Y.,State Key Laboratory of Tree Genetics and Breeding | And 5 more authors.
Planta | Year: 2012

High-throughput RNA sequencing was performed for comprehensively analyzing the transcriptome of the purple sweet potato. A total of 58,800 unigenes were obtained and ranged from 200 nt to 10,380 nt with an average length of 476 nt. The average expression of one unigene was 34 reads per kb per million reads (RPKM) with a maximum expression of 1,935 RPKM. At least 40,280 (68.5%) unigenes were identified to be protein-coding genes, in which 11,978 and 5,184 genes were homologous to Arabidopsis and rice proteins, respectively. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that 19,707 (33.5%) unigenes were classified to 1,807 terms of GO including molecular functions, biological processes, and cellular components and 9,970 (17.0%) unigenes were enriched to 11,119 KEGG pathways. We found that at least 3,553 genes may be involved in the biosynthesis pathways of starch, alkaloids, anthocyanin pigments, and vitamins. Additionally, 851 potential simple sequence repeats (SSRs) were identified in all unigenes. Transcriptome sequencing on tuberous roots of the sweet potato yielded substantial transcriptional sequences and potentially useful SSR markers which provide an important data source for sweet potato research. Comparison of two RNA-sequence datasets from the purple and the yellow sweet potato showed that UDP-glucose-flavonoid 3-O-glucosyltransferase was one of the key enzymes in the pathway of anthocyanin biosynthesis and that anthocyanin-3-glucoside might be one of the major components for anthocyanin pigments in the purple sweet potato. This study contributes to the molecular mechanisms of sweet potato development and metabolism and therefore that increases the potential utilization of the sweet potato in food nutrition and pharmacy. © 2012 Springer-Verlag.

He J.,Kashi Forestry Technology Extension Station | He J.,State Key Laboratory of Tree Genetics and Breeding | Ma Q.-G.,State Key Laboratory of Tree Genetics and Breeding | Yasin X.,State Key Laboratory of Tree Genetics and Breeding | And 3 more authors.
Forest Research | Year: 2013

In order to view the genetic basis of Xinjiang walnut germplasm resourses, the genetic diversity of 30 walnut cultivars (Juglans regia L.) authorized or approved by Xinjiang Uygur Autonomous Region Forest Tree Cultivar Registration Committee were investigated by using FISH-AFLP. Eight pairs of EcoRI+3/MseI+3 primer combinations were used to amplify the genomic DNA. 981 out of totally 1011 AFLP bands were polymorphic and the average percent of polymorphic bands (PPB) was 97.5%. The value of effective number of alleles (Ne) estimated by different primer combinations ranged from 1.1887 to 1.2347 with an average of 1.2085; Nei's gene diversity (H) ranged from 0.1183 to 0.1412 with an average of 0.1297 and Shannon's information index (I) ranged from 0.1846 to 0.2258 with an average of 0.2066, which revealed a moderate level of genetic diversity. Unique fingerprintings for 30 walnut cultivars were established by 8 primer combinations in the research.

Zheng S.-X.,State Key Laboratory of Tree Genetics and Breeding | Zheng S.-X.,Chinese Academy of Forestry | Zhang J.-G.,State Key Laboratory of Tree Genetics and Breeding | Duan A.-G.,State Key Laboratory of Tree Genetics and Breeding | And 3 more authors.
Forest Research | Year: 2013

Ninety progenies representing three half-sib families of Leuce (Populus alba L., Populus canescens (Ait.) Smith, and Populus tremula L.) were analyzed by using microsatellite to study the genetic diversity. 58 alleles and 112 genotypes were identified based on 12 SSR primers. The percentage of polymorphic loci, the Nei's genetic diversity index and Shannon information index were 100%, 0.6485 and 1.234. The coefficient of gene differentiation was 0.3371, which indicated 66.29% of genetic variation resided within the progenies of species. The results from cluster analysis showed that there was abundant genetic variation among the three Leucespecies, the genetic distance among species ranged from 0.3863 to 1.869 and Populus canescens had the most genetic variation.

Meng X.-N.,Northeast Forestry University | Meng X.-N.,Heilongjiang Academy of science | Zhao Y.-S.,Northeast Forestry University | Zheng L.,State Key Laboratory of Tree Genetics and Breeding | Xin Y.,Northeast Forestry University
Chinese Journal of Applied Ecology | Year: 2012

Taking the Pinus sylvestris var. mongolica sand-fixing plantations at different development stages (24-, 29-, 39-, and 43 years old) in Nenjiang Sandy Land as test objects, this paper studied their population structure, understory species composition, and species diversity. No regenerated seedlings were found in all the four P. sylvestris var. mongolica plantations. The mean individual height and DBH of the populations differed significantly with development stage. With the increasing age of the plantations, the proportion of small-sized individuals decreased obviously, while that of large-sized individuals increased, population tended to mature, and the diameter structure except 43 years old plantation was in normal distribution. A total of 33 understory plant species were recorded, belonging to 28 genera and 15 families. Setaria viridis was the dominant species, but its dominance decreased gradually with increasing age of the plantations. With the increase of plantation age, the proportion of annual plants decreased, while that of perennial plants increased. The Simpson index and Pielou index had no significant differences among the different aged plantations, but the richness index, Shannon index, and Alatalo index of 39 years old plantation were significantly higher than those of 24 years old stands, suggesting that the species diversity of the community improved with time.

Sang J.,State Key Laboratory of Tree Genetics and Breeding | Sang J.,Chinese Academy of Forestry | Han X.,State Key Laboratory of Tree Genetics and Breeding | Han X.,Chinese Academy of Forestry | And 8 more authors.
PLoS ONE | Year: 2013

Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions. Although with high sensitivity, specificity and broad dynamic range, this method requires suitable reference genes for transcript normalization in order to guarantee reproducible and meaningful results. In the present study, we evaluated five traditional housekeeping genes and five novel reference genes in Hyperaccumulating ecotype of Sedum alfredii, a well known hyperaccumulator for heavy metals phytoremediation, under Cd, Pb, Zn and Cu stresses of seven different durations. The expression stability of these ten candidates were determined with three programs - geNorm, NormFinder and BestKeeper. The results showed that all the selected reference genes except for SAND could be used for RT-qPCR normalization. Among them UBC9 and TUB were ranked as the most stable candidates across all samples by three programs together. For the least stable reference genes, however, BestKeeper produced different results compared with geNorm and NormFinder. Meanwhile, the expression profiles of PCS under Cd, Pb, Zn and Cu stresses were assessed using UBC9 and TUB respectively, and similar trends were obtained from the results of the two groups. The distinct expression patterns of PCS indicated that various strategies could be taken by plants in adaption to different heavy metals stresses. This study will provide appropriate reference genes for further gene expression quantification using RT-qPCR in Hyperaccumulator S. alfredii. © 2013 Sang et al.

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