State Key Laboratory of Tree Genetics and Breeding

Beijing, China

State Key Laboratory of Tree Genetics and Breeding

Beijing, China

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Xue L.,Chinese Academy of Forestry | Xue L.,State Key Laboratory of Tree Genetics and Breeding | Liu J.,Chinese Academy of Forestry | Liu J.,State Key Laboratory of Tree Genetics and Breeding | And 11 more authors.
Clean - Soil, Air, Water | Year: 2014

A field survey of native herbaceous plants and associated soil in an antimony mining area of Xikuangshan, Hunan Province, China was conducted to identify species that accumulate heavy metals in their tissues. The results indicate that the soils in the mining area were contaminated mainly by As and Sb, at concentrations of 423.77-526.57 and 228.37-445.20mg/kg, respectively. Of the herbaceous plants, Miscanthus sinensis and Imperata cylindrica exhibited Hg and Cd phytoextraction, with bioconcentration factors (BCFs) and translocation factors (TFs) greater than 1. Moreover, Phytolacca americana could be used for the phytostabilization of Pb and Cd (BCF=1.06 and 7.66, respectively), and Cynodon dactylon had considerable potential for As and Sb stabilization (BCF=2.02 and 6.62, respectively). Boehmeria was capable of accumulating Sb and As in its shoots (TF=3.12 and 4.86, respectively). Additionally, the concentration of Sb in the roots of C. dactylon reached 2209.3mg/kg, which is the highest Sb concentration reported in a plant species to date. Our data suggest that native herbaceous plants growing in metal-contaminated sites have phytoremediation potential. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Zhao H.,State Key Laboratory of Tree Genetics and Breeding | Zhao H.,Chinese Academy of Forestry | Zheng Y.-Q.,State Key Laboratory of Tree Genetics and Breeding | Li B.,State Key Laboratory of Tree Genetics and Breeding | And 5 more authors.
Forest Research | Year: 2014

In order to explore the genetic variation among Pinus bungeana populations, their genetic diversity and differentiation level were studied in 21 natural populations by EST-SSR markers. 14 polymorphic loci were detected in 663 individuals among 21 natural populations using 7 pairs of EST-SSR primes. The effective number of alleles (Ne), Shannon's information index (I), observed heterozygosity (Ho), expected heterozygosity (He) and Nei's expected heterozygosity (Nei's) were 1.156 5-1.601 9, 0.133 5-0.492 5, 0.138 4-0.397 3, 0.086-0.342 8, and 0.084 6-0.337 4 respectively. The results of genetic differentiation analysis showed that the average Fst and Nm were 0.215 2 and 0.911 9 respectively, the gene flow was less and the genetic differentiation was high among populations. Central regions of genetic diversity are mainly in west and south of the distribution and the genetic diversity in distribution changed regularly from west to east and from south to north.

Zheng S.-X.,State Key Laboratory of Tree Genetics and Breeding | Zheng S.-X.,Chinese Academy of Forestry | Zhang J.-G.,State Key Laboratory of Tree Genetics and Breeding | He C.-Y.,State Key Laboratory of Tree Genetics and Breeding | And 4 more authors.
Forest Research | Year: 2015

Along the Erqis River, there are various natural Populus forests, which include Section Leuce in Populus, such as Populus alba, Populus canescens and Populus tremula. Clonal diversity and structure were studied using nuclear microsatellite markers. The results showed that clonal reproduction was the primary reproduction strategy of these Populus populations. The populations of P. alba and P. tremula had an abundant clonal diversity and the mean Simpson's index were 0.983 and 0.987, respectively. Compared with the P. tremula, Populus alba had lower genetic diversity and their Shannon' index were 1.068 9 and 0.324 9, Nei' index were 0.505 6 and 0.211 2, respectively. The genetic consistency was high among populations, and variation range were 0.778 1 0.954 4 in P. tremula, 0.975 1 0.994 6 in Populus alba. It suggested they had long-distance gene flow and prevented genetic differentiation among P. alba and P. tremula population. In addition, genetic diversity indicated that the genetic variation occurred within natural populations, which were 95% in P. alba, and 89.98% in P. tremula. ©, 2015, Chinese Academy of Forestry. All right reserved.

Xie F.,East Carolina University | Burklew C.E.,East Carolina University | Yang Y.,Chinese Academy of Forestry | Yang Y.,State Key Laboratory of Tree Genetics and Breeding | And 5 more authors.
Planta | Year: 2012

High-throughput RNA sequencing was performed for comprehensively analyzing the transcriptome of the purple sweet potato. A total of 58,800 unigenes were obtained and ranged from 200 nt to 10,380 nt with an average length of 476 nt. The average expression of one unigene was 34 reads per kb per million reads (RPKM) with a maximum expression of 1,935 RPKM. At least 40,280 (68.5%) unigenes were identified to be protein-coding genes, in which 11,978 and 5,184 genes were homologous to Arabidopsis and rice proteins, respectively. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that 19,707 (33.5%) unigenes were classified to 1,807 terms of GO including molecular functions, biological processes, and cellular components and 9,970 (17.0%) unigenes were enriched to 11,119 KEGG pathways. We found that at least 3,553 genes may be involved in the biosynthesis pathways of starch, alkaloids, anthocyanin pigments, and vitamins. Additionally, 851 potential simple sequence repeats (SSRs) were identified in all unigenes. Transcriptome sequencing on tuberous roots of the sweet potato yielded substantial transcriptional sequences and potentially useful SSR markers which provide an important data source for sweet potato research. Comparison of two RNA-sequence datasets from the purple and the yellow sweet potato showed that UDP-glucose-flavonoid 3-O-glucosyltransferase was one of the key enzymes in the pathway of anthocyanin biosynthesis and that anthocyanin-3-glucoside might be one of the major components for anthocyanin pigments in the purple sweet potato. This study contributes to the molecular mechanisms of sweet potato development and metabolism and therefore that increases the potential utilization of the sweet potato in food nutrition and pharmacy. © 2012 Springer-Verlag.

He J.,Kashi Forestry Technology Extension Station | He J.,State Key Laboratory of Tree Genetics and Breeding | Ma Q.-G.,State Key Laboratory of Tree Genetics and Breeding | Yasin X.,State Key Laboratory of Tree Genetics and Breeding | And 3 more authors.
Forest Research | Year: 2013

In order to view the genetic basis of Xinjiang walnut germplasm resourses, the genetic diversity of 30 walnut cultivars (Juglans regia L.) authorized or approved by Xinjiang Uygur Autonomous Region Forest Tree Cultivar Registration Committee were investigated by using FISH-AFLP. Eight pairs of EcoRI+3/MseI+3 primer combinations were used to amplify the genomic DNA. 981 out of totally 1011 AFLP bands were polymorphic and the average percent of polymorphic bands (PPB) was 97.5%. The value of effective number of alleles (Ne) estimated by different primer combinations ranged from 1.1887 to 1.2347 with an average of 1.2085; Nei's gene diversity (H) ranged from 0.1183 to 0.1412 with an average of 0.1297 and Shannon's information index (I) ranged from 0.1846 to 0.2258 with an average of 0.2066, which revealed a moderate level of genetic diversity. Unique fingerprintings for 30 walnut cultivars were established by 8 primer combinations in the research.

Zheng S.-X.,State Key Laboratory of Tree Genetics and Breeding | Zheng S.-X.,Chinese Academy of Forestry | Zhang J.-G.,State Key Laboratory of Tree Genetics and Breeding | Duan A.-G.,State Key Laboratory of Tree Genetics and Breeding | And 3 more authors.
Forest Research | Year: 2013

Ninety progenies representing three half-sib families of Leuce (Populus alba L., Populus canescens (Ait.) Smith, and Populus tremula L.) were analyzed by using microsatellite to study the genetic diversity. 58 alleles and 112 genotypes were identified based on 12 SSR primers. The percentage of polymorphic loci, the Nei's genetic diversity index and Shannon information index were 100%, 0.6485 and 1.234. The coefficient of gene differentiation was 0.3371, which indicated 66.29% of genetic variation resided within the progenies of species. The results from cluster analysis showed that there was abundant genetic variation among the three Leucespecies, the genetic distance among species ranged from 0.3863 to 1.869 and Populus canescens had the most genetic variation.

Li Q.-H.,Chinese Academy of Forestry | Li Q.-H.,State Key Laboratory of Tree Genetics and Breeding | Xu J.,Chinese Academy of Forestry | Wang S.-X.,Chinese Academy of Forestry | And 4 more authors.
Journal of Food, Agriculture and Environment | Year: 2012

Nitraria tangutorum, a common sand-fixation plant in the desertificated areas of northern China, is widely distributed in the northeastern Ulan Buh Desert. To better understand the population ecological process and local community developmental changes of Nitraria tangutorum, it is important to study its population distribution and gradient changes. We studied the populations and communities of Nitraria tangutorum in four sample plots in the northeastern Ulan Buh Desert, which were located between the Yellow River bank and the Langshan piedmont. We analyzed the survey results with the point pattern analysis method to elucidate the population and individual spatial distribution pattern of Nitraria tangutorum. We further explored the relationship between the community and the ecological environment through species-quadrat ordination analysis. Our results indicated that the population spatial distribution pattern of Nitraria tangutorum is closely related to the spatial scale, plant biological characteristics, and ecological environment. The populations of Nitraria tangutorum were regularly distributed at small spatial scales, but can become randomly distributed when the spatial scale reaches a threshold. Compared to the populations close to the Yellow River, those close to Langshan Mountain showed greater extents of regular distribution at small scales and random distribution at large scales. With the gradient alteration of the ecological environment of the sample plots, the plant species distribution of the Nitraria tangutorum communities also showed a gradient changing pattern from the Yellow River bank to the Langshan piedmont. Our results elucidated the spatial distribution difference of dominant Nitraria tangutorum populations between the Yellow River bank and the Langshan piedmont, as well as the gradient changing pattern of the Nitraria tangutorum communities.

Meng X.-N.,Northeast Forestry University | Meng X.-N.,Heilongjiang Academy of science | Zhao Y.-S.,Northeast Forestry University | Zheng L.,State Key Laboratory of Tree Genetics and Breeding | Xin Y.,Northeast Forestry University
Chinese Journal of Applied Ecology | Year: 2012

Taking the Pinus sylvestris var. mongolica sand-fixing plantations at different development stages (24-, 29-, 39-, and 43 years old) in Nenjiang Sandy Land as test objects, this paper studied their population structure, understory species composition, and species diversity. No regenerated seedlings were found in all the four P. sylvestris var. mongolica plantations. The mean individual height and DBH of the populations differed significantly with development stage. With the increasing age of the plantations, the proportion of small-sized individuals decreased obviously, while that of large-sized individuals increased, population tended to mature, and the diameter structure except 43 years old plantation was in normal distribution. A total of 33 understory plant species were recorded, belonging to 28 genera and 15 families. Setaria viridis was the dominant species, but its dominance decreased gradually with increasing age of the plantations. With the increase of plantation age, the proportion of annual plants decreased, while that of perennial plants increased. The Simpson index and Pielou index had no significant differences among the different aged plantations, but the richness index, Shannon index, and Alatalo index of 39 years old plantation were significantly higher than those of 24 years old stands, suggesting that the species diversity of the community improved with time.

Sang J.,State Key Laboratory of Tree Genetics and Breeding | Sang J.,Chinese Academy of Forestry | Han X.,State Key Laboratory of Tree Genetics and Breeding | Han X.,Chinese Academy of Forestry | And 8 more authors.
PLoS ONE | Year: 2013

Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions. Although with high sensitivity, specificity and broad dynamic range, this method requires suitable reference genes for transcript normalization in order to guarantee reproducible and meaningful results. In the present study, we evaluated five traditional housekeeping genes and five novel reference genes in Hyperaccumulating ecotype of Sedum alfredii, a well known hyperaccumulator for heavy metals phytoremediation, under Cd, Pb, Zn and Cu stresses of seven different durations. The expression stability of these ten candidates were determined with three programs - geNorm, NormFinder and BestKeeper. The results showed that all the selected reference genes except for SAND could be used for RT-qPCR normalization. Among them UBC9 and TUB were ranked as the most stable candidates across all samples by three programs together. For the least stable reference genes, however, BestKeeper produced different results compared with geNorm and NormFinder. Meanwhile, the expression profiles of PCS under Cd, Pb, Zn and Cu stresses were assessed using UBC9 and TUB respectively, and similar trends were obtained from the results of the two groups. The distinct expression patterns of PCS indicated that various strategies could be taken by plants in adaption to different heavy metals stresses. This study will provide appropriate reference genes for further gene expression quantification using RT-qPCR in Hyperaccumulator S. alfredii. © 2013 Sang et al.

Liu Z.X.,State Key Laboratory of Tree Genetics and Breeding | Zhang H.X.,State Key Laboratory of Tree Genetics and Breeding | Yang X.Y.,State Key Laboratory of Tree Genetics and Breeding | Liu T.,State Key Laboratory of Tree Genetics and Breeding | Di W.B.,Beijing Forestry Survey and Design Institute
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2014

As a salt-tolerant shrub/ tree species, Elaeagnus angustifolia L. is widely planted for afforestation in many marginal lands or environmentally harsh conditions in northern China. Although E. angustifolia is well known for its strong adaptation to harsh conditions, the underpinning physiological mechanisms associated with ion transport and homeostasis under high-salt conditions have not been revealed. Has it developed some physiological mechanisms to avoid the high Na+ in soil or sequester the Na+ in some specific tissues or organs? The use of E. angustifolia to answer these questions can greatly enhance our understanding of the general physiological mechanisms that plants deploy to combat the environmental challenges. To unravel the underlying physiological mechanisms responsible for the extra-ordinary adaptation to high salt in E. angustifolia, we used the well-controlled water culture experiment in greenhouse to investigate the biomass accumulation, and the absorption, transportation and allocation of multiple ions including K+, Na+, Ca2+ and Mg2+ in different plant tissues (roots, stems and leaves) of E. angustifolia seedlings upon being challenged by different NaCl concentrations (0, 100 and 200 mmol/ L) for 30 days. Interestingly, the root growth was stimulated to a different extent by salt stress. The biomass accumulation of E. angustifolia seedlings was not obviously affected by 100 mmol/ L NaCl stress, whereas it was significantly inhibited by 200 mmol/ L NaCl stress. Compared with non-salt control, the K+-Na+ selective transportation coefficients (SK, Na) and Ca2+-Na+ selective transportation coefficients (SCa, Na) of different plant tissues (roots, stems and leaves) under two salt concentrations were all significantly elevated, while the contents of K+, Ca2+ and Mg2+, and the ratios of K+ / Na+, Ca2+ / Na+ and Mg2+ / Na+ in the three plant tissues were all significantly decreased. The Na+ concentration and net Na+ accumulation in 200 mmol/ L NaCl-stressed seedlings'roots were 22.15 mg/ g DW and 1.87 mg/ plant, respectively, which were 16.20 and 20.06 times higher than that in the control roots, respectively. The concentration and the accumulating amplitude of Na+ in roots were more conspicuous than any of other two tissues, implicating that roots may contribute vitally to the observed salt-tolerance of E. angustifolia. The Na+ concentration in stems and leaves of 200 mmol/ L NaCl-stressed seedlings increased to 5.15 and 7.71 mg/ g DW, which were 7.22 and 9.58 times the content in corresponding control, respectively, and net Na+ accumulation in 200 mmol/ L NaCl-stressed seedlings'shoots was 3.29 mg/ plant (5.45 times as much as in control shoot). However, all seedlings stressed by two salt concentrations exhibited a normal growth, no typical salt-damaged symptoms like succulent shoot and abscised leaves in treated seedlings were observed, indicating that shoots (including stems and leaves) can tolerate high concentration's Na+ stress. In conclusion, our findings suggested that the salt-adaptation mechanisms of E. angustifolia are root salt-rejection and shoot salt-tolerance, which are primarily implemented by root growth stimulation, root Na+ accumulation and restriction, and shoot Na+ endurance, and are also correlated with a remarkably increased ability of K+ and Ca2+ selective transportation in roots, stems and leaves.

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