Zha D.-M.,Chinese Academy of Agricultural Sciences |
Zha D.-M.,Jiangsu University of Science and Technology |
Zha D.-M.,State Key Laboratory of Special Economic Animal Molecular Biology |
Zha D.-M.,Key Laboratory of Special Economic Animal Germplasm Resources and Genetic Improvement |
And 6 more authors.
Food Chemistry | Year: 2011
Attempts were made to establish one-step multiplex PCR assay for the identification of the widely used species in deer products (sika deer, wapiti, red deer and reindeer). Primers were designed from tandem repeat region of D-loop and well-conserved region of 16S rDNA after alignment of the available sequences in the GenBank database. The primers generated specific fragments of 307 bp in length for sika deer, 307 and 246 bp for wapiti, 272 bp for Tarim red deer, 230 bp for red deer and 141 bp for reindeer, respectively. The detection limit was 0.05 ng for sika deer and wapiti, 0.1 ng for Tarim red deer, 0.5 ng for red deer and 0.02 ng for reindeer. The results demonstrated that the fraudulent phenomenon is epidemic in the substitution of deer products, in especially antler, penis, foetus and tendon products. Hence, this multiplex PCR provided a useful and sensitive technique to identify the sources of deer products. © 2011 Elsevier Ltd. All rights reserved.
Zha D.,Chinese Academy of Agricultural Sciences |
Zha D.,Jiangsu University of Science and Technology |
Zha D.,State Key Laboratory of Special Economic Animal Molecular Biology |
Zha D.,Key Laboratory of Special Economic Animal Germplasm Resources and Genetic Improvement |
And 6 more authors.
Food Control | Year: 2010
Attempts were made to established one-step multiplex PCR assay for the fraud identification of the mostly used species in deer products (bovine, ovine, porcine and poultry). Primers were selected from published papers or designed in the well-conserved region of tRNA-Val and 16S rRNA mitochondrial genes after alignment of the available sequences in the GenBank database. The primers generated specific fragments of 124, 183, 225 and 290. bp length for bovine, poultry, ovine and porcine, respectively. The detection limit was 1. ng for porcine and ovine primers, 5. ng for poultry primers and 0.5. ng for bovine primers. The results demonstrated that fraud phenomena are very epidemic in the deer products, especially heart, blood, penis and antler products. The multiplex PCR described in this study, proved to be very sensitive and reliable in species identification, could be considered as a further improvement of traditional methods based assay for the identification of deer products. © 2010 Elsevier Ltd.
Tu J.,Chinese Academy of Agricultural Sciences |
Tu J.,State Key Laboratory of Special Economic Animal Molecular Biology |
Tu J.,Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction |
Si F.,Chinese Academy of Agricultural Sciences |
And 10 more authors.
Mitochondrial DNA | Year: 2014
Muscovy duck (Cairina moschata) is the most popular meat duck, and it can be crossed with most breeds of domestic ducks in captivity to produce sterile hybrids. In this study, complete mitochondrial genome of the Muscovy duck was sequenced by long and accurate polymerase chain reaction (LA-PCR) as well as the primer walking sequence method. The entire mitochondrial genome of Muscovy duck was 16,610 bp in length, gene composition and arrangement conformed to most bird, which contained the typical structure of 22 tRNAs, 2 rRNAs, 13 protein-coding genes and a non-coding region. The characteristic of the mitochondrial genome was analyzed in detail. Our complete mitochondrial genome sequence will be useful for phylogenetics, and be available as basic data for the breeding and genetics. © 2014 Informa UK Ltd.
Zhang T.-T.,Chinese Academy of Agricultural Sciences |
Zhang T.-T.,State Key Laboratory of Special Economic Animal Molecular Biology |
Zhang Z.-Q.,Chinese Academy of Agricultural Sciences |
Gao X.-H.,Chinese Academy of Agricultural Sciences |
And 2 more authors.
Journal of Animal Physiology and Animal Nutrition | Year: 2013
Summary: This study investigated digestibilities of nutrients and feed efficiency in female mink at the different dietary protein levels during the mink growth period. Effects of dietary protein on growth performance of minks were also measured. Sixty 45-day-old healthy female minks were randomly assigned to 6 treatment groups with 10 animals in each group. Animals were fed diets varying in protein levels: 28% (Group I), 30% (Group II), 32% (Group III), 34% (Group IV), 36% (Group V) and 38% (Group VI), respectively. The digestibilities of key nutrients were determined on Day 14 after initiating the experiment and the last 3days. From the beginning of the study, body weight and feed intake were weighed and recorded every other week in order to calculate the average daily bodyweight gain and the feed efficiency. The trial had demonstrated that nitrogen intake was greatly significantly different, which was affected by dietary protein levels (p<0.001). Growth performance of minks was impaired when dietary protein level was at 28%. When dietary protein level was at 34%, minks had the best daily gains, feed efficiency, and digestibilities of some key nutrients. © 2012 Blackwell Verlag GmbH.