State Key Laboratory of Rice Biology

State Key Laboratory of Rice Biology

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Li X.-X.,Chongqing Normal University | Zheng J.,Chongqing Normal University | Zhou J.-J.,Chongqing Normal University | Qin X.-J.,Chongqing Normal University | And 6 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2016

The study was to elucidate the genetic information of QTL determining important agronomical traits and obtain the linkage marker of QTL underlying japonica variety with high yield and heat tolerance character with by method of QTL mapping, and provide some useful information for the further molecular mechanism of japonica variety and its genetic improvement. A F2 population derived from the cross between japonica 'Rejing35' and indica 'Xieqingzao B' containing 226 lines was used to perform the QTL analysis. A genetic linkage map containing 156 SSR was constructed by MapMaker3.0 software; the methods to ANOVA, Correlation, CIM and MIM were applied to perform the phenotypic data analysis and QTL mapping using DPS and Win-QTLCart 2.50 software. The reliable genomic regions of major QTL were confirmed by comparison with the publicly available QTL data from the website of www.gramene.org and the previously published literature. T-test analysis for thirteen traits showed that a significant difference was lied between 'Rejing35' and 'XieqingzaoB', a crazy segregation occurs in these traits, and a normal distribution of phenotypic values for all traits was observed in the F2 population, indicated that these traits were controlled by multiple genes (QTL). Twenty-four QTLs were detected on chromosome 1, 2, 3, 4, 5, 6, 9, 11 and 12 for all traits, respectively, with the LOD values ranged from 3.02 to 12.23, the additive effect from -31.33 to 18.01, the dominative effect from -21.15 to 50.54, and the range of individual QTL explaining phenotypic variation was from 7.15% to 70.56%. Of these, there 8 pleiotropic QTLs were located repeatedly for multiple traits on chromosome 1, 2, 4, 5, 9 and 11, respectively, five pairs of epistatic QTLs were detected, these QTLs were not located on the same genomic region to that of the previously published QTLs. 'Rejing35' shows the unique genetic model, The genetic model of additive/dominant QTL underlying agronomic trait is more important than that of epistatic QTL, the genomic regions of all QTLs were not in the same genomic region to the previously published QTLs, there 8 pleiotropic QTLs were detected repeatedly for multiple traits, five pairs of epistatic QTLs were detected for three traits. © 2016, Science Press. All right reserved.


Zhou Y.,State Key Laboratory of Rice Biology | Lin X.-W.,State Key Laboratory of Rice Biology | Begum M.-A.,State Key Laboratory of Rice Biology | Zhang C.-H.,State Key Laboratory of Rice Biology | And 7 more authors.
Insect Molecular Biology | Year: 2017

The neutral sphingomyelinase (nSMase) 1 homologue gene LsSMase was cloned from Laodelphax striatellus, a direct sap-sucker and virus vector of gramineous plants, and expressed via a Bac to Bac baculovirus expression system. The LsSMase-enhanced green fluorescent protein fusion protein was located in the endoplasmic reticulum in a similar manner to mammalian nSMase 1. The biochemical properties of LsSMase were determined in detail. The optimal pH and temperature for recombinant LsSMase were 8 and 37°C, respectively. LsSMase was an Mg2+ or Mn2+ dependent enzyme, but different concentration of each were needed. The activity of LsSMase was significantly stimulated by Ethylene glycol bis(2-aminoethyl ether)tetraacetic acid (EGTA), whereas it was inhibited by ethylenediaminetetraacetic acid. Millimolar concentrations of Zn2+ completely inhibited LsSMase. The reducing agents dithiothreitol and β-mercaptoethanol varied in their effects on activity. Phospholipids were not found to stimulate LsSMase. © 2017 The Royal Entomological Society.


Zhou Y.,State Key Laboratory of Rice Biology | Zhou Y.,Zhejiang University | Lin X.-W.,State Key Laboratory of Rice Biology | Lin X.-W.,Zhejiang University | And 13 more authors.
Biochimie | Year: 2011

Ceramidase plays an important role in regulating the metabolism of sphingolipids, such as ceramide, sphingosine (SPH), and sphingosine-1-phosphate (S1P), by controlling the hydrolysis of ceramide. Here we report the cloning and biochemical characterization of a neutral ceramidase from the red flour beetle Tribolium castaneum which is an important storage pest. The Tribolium castaneum neutral ceramidase (Tncer) is a protein of 696 amino acids. It shares a high degree of similarity in protein sequence to neutral ceramidases from various species. Tncer mRNA levels are higher in the adult stage than in pre-adult stages, and they are higher in the reproductive organs than in head, thorax, and midgut. The mature ovary has higher mRNA levels than the immature ovary. Tncer is localized to the plasma membrane. It uses various ceramides (D-erythro-C 6, C12, C16, C18:1, and C 24:1-ceramide) as substrates and has an abroad pH optimum for its in vitro activity. Tncer has an optimal temperature of 37°C for its in vitro activity. Its activity is inhibited by Fe2+. These results suggest that Tncer has distinct biochemical properties from neutral ceramidases from other species. © 2011 Elsevier Masson SAS. All rights reserved.


Yang Q.,State Key Laboratory of Rice Biology | Yang Q.,Zhejiang University | Yang Q.,Jiangsu Academy of Agricultural Sciences | Gong Z.-J.,State Key Laboratory of Rice Biology | And 13 more authors.
Cellular and Molecular Life Sciences | Year: 2010

Ceramidases catalyze the hydrolysis of ceramides to generate sphingosine (SPH) and fatty acids, and ceramide metabolism is implicated in various biological responses in Drosophila melanogaster. Here we report the cloning, biochemical characterization, and functional analysis of a Drosophila alkaline ceramidase (Dacer). Dacer, a membrane-bound protein of 284 amino acids, shares homology with yeast and mammalian alkaline ceramidases. Overexpression of Dacer in High Five insect cells increases ceramidase activity in the alkaline pH range, indicating that Dacer is a bona fide alkaline ceramidase. Dacer mRNA is highly expressed in the midgut and at the pupal stage. An inactivation of Dacer by insertional mutagenesis increases the levels of ceramides in both Drosophila pupae and adult flies. Dacer inactivation increases Drosophila pre-adult development time, lifespan, and anti-oxidative stress capacity. Collectively, these results suggest that Dacer plays an important role in the Drosophila development and longevity by controlling the metabolism of ceramides. © 2010 Birkhäuser Verlag.


Fu B.-X.,State Key Laboratory of Rice Biology | Bellis G.A.,Khan Research Laboratories | Hong J.,Zhejiang University | Wang J.-R.,Zhejiang University | And 4 more authors.
Microscopy Research and Technique | Year: 2012

The antennal sensilla of both genders of macropterous and brachypterous adults of the small brown planthopper, Laodelphax striatellus (Fallén) (Hemiptera: Delphacidae) were examined using light and scanning electron microscopy. Scanning electron microscopy revealed seven types of antennal sensilla in adult L. striatellus which were not evenly distributed on all antennal segments. Sensilla chaetica, a sensillum campaniformium and a Böhm bristle were found on the scape. Sensilla chaetica, sensilla trichodea, sensilla placodea which always present as plaque organs, sensilla basiconica and a sensillum campaniformium were present on the pedicel. Three sensilla basiconica and one sensillum coeloconicum containing two sensory pegs were located on the swollen sensory region of the basal flagellum. Pores observed on the surface of s. trichodea and s. placodea suggest these organs probably play a role in olfaction, whereas the aporous s. chaetica with flexible sockets probably function as mechanoreceptors. The aporous s. basiconica with inflexible sockets are probable to be thermo-hygroreceptors while the Böhm bristle and s. campaniformia may act as antennal proprioceptors. The function of s. coeloconicum remains uncertain. The numerical dominance of antennal olfactory receptors suggests olfaction is an important function of the antenna in L. striatellus. Although a small degree of sexual/wing dimorphism was observed in the numbers of sensilla and in the length and width of antennae and antennal segments, the basic shape and structure of the antennae and antennal sensilla did not differ between the gender or wing form in L. striatellus. Microsc. © 2012 Wiley Periodicals, Inc.


Li B.,State Key Laboratory of Rice Biology | Fang Y.,State Key Laboratory of Rice Biology | Zhang G.,State Key Laboratory of Rice Biology | Yu R.,Zhejiang University of Technology | And 4 more authors.
Plant Pathology Journal | Year: 2010

The Burkholderia cepacia complex isolates causing bacterial fruit rot of apricot were characterized by speciesspecific PCR tests, recA-HaeIII restriction fragment length polymorphism (RFLP) assays, rep-PCR genomic fingerprinting, recA gene sequencing, and multilocus sequence typing (MLST) analysis. Results indicated that the isolates Bca 0901 and Bca 0902 gave positive amplifications with primers specific for B. vietnamiensis while the two bacterial isolates showed different recARFLP and rep-PCR profiles from those of B. vietnamiensis strains. In addition, the two bacterial isolates had a higher proteolytic activity compared with that of the non-pathogenic B. vietnamiensis strains while no cblA and esmR marker genes were detected for the two bacterial isolates and B. vietnamiensis strains. The two bacterial isolates were identified as Burkholderia seminalis based on recA gene sequence analysis and MLST analysis. Overall, this is the first characterization of B. seminalis that cause bacterial fruit rot of apricot. © The Korean Society of Plant Pathology.


E Z.G.,China National Rice Research Institute | Zhang Y.P.,China National Rice Research Institute | Zhou J.H.,Bioinfo Groups | Zhou J.H.,Nantong University | And 2 more authors.
Genetics and Molecular Research | Year: 2014

The basic leucine zipper (bZIP) genes encode transcription factors involved in the regulation of various biological processes. Similar to WRKY, basic helix-loop-helix, and several other groups of proteins, the bZIP proteins form a superfamily of transcription factors that mediate plant stress responses. In this review, we present the roles of bZIP proteins in multiple biological processes that include pathogen defense; responses to abiotic stresses; seed development and germination; senescence; and responses to salicylic, jasmonic, and abscisic acids in rice. We also examined the characteristics of the bZIP proteins and their genetic composition. To ascertain the evolutionary changes in and functions of this supergene family, we performed an exhaustive comparison among the 89 rice bZIP genes that were previously described and those more recently listed in the MSU Rice Genome Annotation Project Database using a Hidden Markov Model. We excluded 3 genes from the list, resulting in a total of 86 bZIP genes in japonica rice. © FUNPEC-RP.


E Z.-G.,China National Rice Research Institute | Ge L.,China National Rice Research Institute | Wang L.,China National Rice Research Institute | Wang L.,State Key Laboratory of Rice Biology
Plant Growth Regulation | Year: 2012

Adventitious roots account for the majority of the rice root system and play an irreplaceable role in rice growth and development. Rice adventitious roots are formed by division of the innermost ground meristem cells in the central cylinder, and some lateral roots are observable in the adventitious root system. Multiple hormones have been implicated in the regulation of root development. Auxin is involved in the initiation of adventitious roots, whereas cytokinin inhibits adventitious root initiation, but promotes adventitious root elongation. Other phytohormones such as nitric oxide, ethylene, brassinosteroid, jasmonic acid and gibberellin may be also involved in regulating adventitious root initiation and development. Additionally, more than 600 root development related quantitative trait loci (QTLs) have been located by QTL analysis of root traits. © 2012 Springer Science+Business Media B.V.


PubMed | State Key Laboratory of Rice Biology
Type: Journal Article | Journal: Insect molecular biology | Year: 2013

Ceramidases are a group of enzymes that catalyse hydrolysis of ceramides to generate fatty acid and sphingosine. In this study, we report the cloning and characterization of the rice small brown planthopper Laodelphax striatellus neutral ceramidase (nCDase), LsnCer. LsnCer was identified by sequencing the transcriptome of L. striatellus and is a protein of 717 amino acids with a predicted molecular weight of 79.3kDa. Similarly to other known nCDases, the optimum pH for LsnCer is 8.0 and the optimum temperature is 37C for its in vitro activity. LsnCer activity is inhibited by Zn(2+) significantly and Fe(2+) slightly. LsnCer has broad substrate specificity with a preference for ceramides with a medium acyl-chain or a monounsaturated long acyl-chain. Infection with rice strip virus (RSV) or treatment with insecticides significantly increased LsnCer mRNA expression and its enzymatic activity in L.striatellus. These results suggest that LsnCer is a bona fidenCDase that may have a role in adaption of L.striatellus to environmental stresses.


PubMed | State Key Laboratory of Rice Biology
Type: Journal Article | Journal: Cellular and molecular life sciences : CMLS | Year: 2010

Ceramidases catalyze the hydrolysis of ceramides to generate sphingosine (SPH) and fatty acids, and ceramide metabolism is implicated in various biological responses in Drosophila melanogaster. Here we report the cloning, biochemical characterization, and functional analysis of a Drosophila alkaline ceramidase (Dacer). Dacer, a membrane-bound protein of 284 amino acids, shares homology with yeast and mammalian alkaline ceramidases. Overexpression of Dacer in High Five insect cells increases ceramidase activity in the alkaline pH range, indicating that Dacer is a bona fide alkaline ceramidase. Dacer mRNA is highly expressed in the midgut and at the pupal stage. An inactivation of Dacer by insertional mutagenesis increases the levels of ceramides in both Drosophila pupae and adult flies. Dacer inactivation increases Drosophila pre-adult development time, lifespan, and anti-oxidative stress capacity. Collectively, these results suggest that Dacer plays an important role in the Drosophila development and longevity by controlling the metabolism of ceramides.

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