State Key Laboratory of Phytochemistry and Plant Resources in West China

Shatin, Hong Kong

State Key Laboratory of Phytochemistry and Plant Resources in West China

Shatin, Hong Kong
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Chan B.C.L.,Chinese University of Hong Kong | Chan B.C.L.,State Key Laboratory of Phytochemistry and Plant Resources in West China | Lee H.Y.,Chinese University of Hong Kong | Siu W.S.,Chinese University of Hong Kong | And 9 more authors.
Journal of Pharmacy and Pharmacology | Year: 2014

Objectives Mast cells are believed to contribute to the pathogenesis of osteoporosis as their number is increased in osteoporotic bones. Herba Epimedii, Fructus Ligustri Lucidi and Fructus Psoraleae are three Chinese herbs traditionally for tonifying the 'kidney system' and a herbal formula (ELP) containing the respective herbs at the weight ratio of 5: 4: 1 was shown to prevent osteoporosis. This study evaluated if suppression of mast cell accumulation and activity contribute to the anti-osteoporotic action of ELP. Methods The herbs were boiled under reflux to produce the aqueous extract that was further concentrated under reduced pressure and lyophilized. An in-vivo rat osteoporosis model using hind limb unloading was employed for studying the accumulation of mast cells. The human mast cell line, LAD2, was employed to evaluate the mast cell modulating action of ELP. Key findings Mast cell number in the tibiae of hind limb unloaded rats increased significantly during the course of osteoporosis. ELP treatment (10 g/kg/day) prevented both osteoporosis and mast cell accumulation in these rats. Furthermore, ELP significantly inhibited histamine and tumour necrosis factor-α release from LAD2 cells. Conclusion Mast cells contributed to hormone independent osteoporosis. The suppression of mast cell accumulation and activation may contribute to the anti-osteoporotic action of ELP. © 2013 Royal Pharmaceutical Society.


Tibpromma S.,CAS Kunming Institute of Botany | Tibpromma S.,Mae Fah Luang University | Tibpromma S.,World Agroforestry Center | Tibpromma S.,Mushroom | And 173 more authors.
Fungal Diversity | Year: 2017

This is a continuity of a series of taxonomic and phylogenetic papers on the fungi where materials were collected from many countries, examined and described. In addition to extensive morphological descriptions and appropriate asexual and sexual connections, DNA sequence data are also analysed from concatenated datasets to infer phylogenetic relationships and substantiate systematic positions of taxa within appropriate ranks. Wherever new species or combinations are proposed, we apply an integrative approach using morphological and molecular data as well as ecological features wherever applicable. Notes on 112 fungal taxa are compiled in this paper including Biatriosporaceae and Roussoellaceae, Didysimulans gen. nov., 81 new species, 18 new host records and new country records, five reference specimens, two new combinations, and three sexual and asexual morph reports. The new species are Amanita cornelii, A. emodotrygon, Angustimassarina alni, A. arezzoensis, A. italica, A. lonicerae, A. premilcurensis, Ascochyta italica, A. rosae, Austroboletus appendiculatus, Barriopsis thailandica, Berkleasmium ariense, Calophoma petasitis, Camarosporium laburnicola, C. moricola, C. grisea, C. ossea, C. paraincrustata, Colletotrichum sambucicola, Coprinopsis cerkezii, Cytospora gelida, Dacrymyces chiangraiensis, Didysimulans italica, D. mezzanensis, Entodesmium italica, Entoloma magnum, Evlachovaea indica, Exophiala italica, Favolus gracilisporus, Femsjonia monospora, Fomitopsis flabellata, F. roseoalba, Gongronella brasiliensis, Helvella crispoides, Hermatomyces chiangmaiensis, H. chromolaenae, Hysterium centramurum, Inflatispora caryotae, Inocybe brunneosquamulosa, I. luteobrunnea, I. rubrobrunnea, Keissleriella cirsii, Lepiota cylindrocystidia, L. flavocarpa, L. maerimensis, Lophiotrema guttulata, Marasmius luculentus, Morenoina calamicola, Moelleriella thanathonensis, Mucor stercorarius, Myrmecridium fluviae, Myrothecium septentrionale, Neosetophoma garethjonesii, Nigrograna cangshanensis, Nodulosphaeria guttulatum, N. multiseptata, N. sambuci, Panus subfasciatus, Paraleptosphaeria padi, Paraphaeosphaeria viciae, Parathyridaria robiniae, Penicillium punicae, Phaeosphaeria calamicola, Phaeosphaeriopsis yuccae, Pleurophoma italica, Polyporus brevibasidiosus, P. koreanus, P. orientivarius, P. parvovarius, P. subdictyopus, P. ulleungus, Pseudoasteromassaria spadicea, Rosellinia mearnsii, Rubroboletus demonensis, Russula yanheensis, Sigarispora muriformis, Sillia italica, Stagonosporopsis ailanthicola, Strobilomyces longistipitatus, Subplenodomus galicola and Wolfiporia pseudococos. The new combinations are Melanomma populina and Rubroboletus eastwoodiae. The reference specimens are Cookeina tricholoma, Gnomoniopsis sanguisorbae, Helvella costifera, Polythrincium trifolii and Russula virescens. The new host records and country records are Ascochyta medicaginicola, Boletellus emodensis, Cyptotrama asprata, Cytospora ceratosperma, Favolaschia auriscalpium, F. manipularis, Hysterobrevium mori, Lentinus sajor-caju, L. squarrosulus, L. velutinus, Leucocoprinus cretaceus, Lophiotrema vagabundum, Nothophoma quercina, Platystomum rosae, Pseudodidymosphaeria phlei, Tremella fuciformis, Truncatella spartii and Vaginatispora appendiculata and three sexual and asexual morphs are Aposphaeria corallinolutea, Dothiora buxi and Hypocrella calendulina. © 2017, School of Science.


Zhu Q.-C.,CAS Guangzhou Institute of Biomedicine and Health | Zhu Q.-C.,Nagasaki University | Wang Y.,CAS Guangzhou Institute of Biomedicine and Health | Wang Y.,South China United Vaccine Institute | And 8 more authors.
European Journal of Pharmaceutical Sciences | Year: 2011

In recent years, enterovirus 71 (EV71) infections have caused an increasing epidemic in young children, accompanying with more severe nervous system disease and more deaths. Unfortunately, there is no specific medication for it so far. Here we investigated the anti-EV71 activity of chrysosplenetin and penduletin, two o-methylated flavonols isolated from the leaves of Laggera pterodonta. These two compounds were found to have strong activity in vitro against EV71 with low cytotoxicity. In the cytopathic effect (CPE) inhibition assays, both plaque reduction assay and virus yield inhibition assay, the compounds showed a similar 50% inhibitory concentration (IC 50) value of about 0.20 μM. The selectivity indices (SI) of chrysosplenetin and penduletin were 107.5 and 655.6 in African green monkey kidney (Vero) cells, and 69.5 and 200.5 in human rhabdomyosarcoma (RD) cells, accordingly. The preliminary mechanism analysis indicates that they function not through blocking virus entry or inactivating virus directly but inhibiting viral RNA replication. In the time-of-addition assay, both compounds inhibited progeny virus production and RNA replication by nearly 100% when introduced within 4 h post infection. In addition to EV71, both compounds inhibited several other human enteroviruses with similar efficacy. These findings provide a significant lead for the discovery of anti-EV71 drug. © 2011 Elsevier B.V. All rights reserved.

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