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Zhu D.-F.,Kunming University of Science and Technology | Nian Y.,State Key Laboratory of Phytochemistry and Plant Resources in West China | Wang H.-Y.,State Key Laboratory of Phytochemistry and Plant Resources in West China | Zhang Z.-R.,State Key Laboratory of Phytochemistry and Plant Resources in West China | And 3 more authors.
Chinese Journal of Natural Medicines | Year: 2014

Aim: To study the 9, 19-cycloartane triterpenes from the roots of Cimicifuga foetida. Method: Chromatographic separations by silica gel, C18 reversed phase silica gel, and high-performance liquid chromatography (HPLC) were used. All of the structures were elucidated on the basis of spectroscopic analysis and chemical methods. Results: Five 9, 19-cycloartane triterpenes, (3β, 12β, 15α, 24. R)-12, 2'-diacetoxy-24, 25-epoxy-15-hydroxy-16, 23-dione-3- O-α-L-arabinopyranoside (1), actein (2), 23- epi-26-deoxyactein (3), asiaticoside B (4), and 12β-hydroxycimigenol (5) were isolated from the roots of Cimicifuga foetida. Conclusion: Compound 1 is a new triterpene with two acetoxy groups at C-2' and C-12. © 2014 China Pharmaceutical University.


Liu C.-L.,Chinese University of Hong Kong | Liu C.-L.,State Key Laboratory of Phytochemistry and Plant Resources in West China | Tam J.C.W.,Chinese University of Hong Kong | Tam J.C.W.,State Key Laboratory of Phytochemistry and Plant Resources in West China | And 11 more authors.
Wound Repair and Regeneration | Year: 2013

The emergence of electric cell-substrate impedance sensing (ECIS) technology has provided new insight in advanced cell behavioral study by its nanometer sensitivity, precise electrical wounds generation, and high reproducibility that can be monitored in real time in a noninvasive way. However, little is known regarding pro-angiogenic agents in wound healing studies using endothelial cells evaluated with ECIS technology. Our previous studies showed a prominent wound healing effect of a two-herb formula (NF3) comprising of Astragali Radix and Rehmanniae Radix in a rat chronic wound model through actions including angiogenesis. Here we further investigated the angiogenic effect and its underlying molecular mechanism through proliferation, motility, and tubule formation of human vascular endothelial cells (HECV) using ECIS technology. It was first shown that HECV treated with NF3 had a higher resistance than that of control using ECIS cell attachment and cell migration model (p < 0.01). We further validated in a scratch assay that NF3 treatment significantly stimulated HECV cell migration (p < 0.01-0.05). Also, NF3-treated HECV were observed to develop into a significantly more branched tubular structure when compared with control (p < 0.05-0.01). Meanwhile, Western blot analysis of NF3-treated HECV revealed the activated expression of p-Akt, and mitogen-activated protein (MAP) kinases for p-ERK, p-p38, and p-JNK. We propose that the effect of NF3 in the promotion of endothelial cell migration and tubule formation could be mediated through pathways involving p-Akt and activated MAP kinases. Hence, we demonstrated the complexity of the angiogenic effect activated by NF3 molecularly and functionally. NF3 treatment could offer therapeutic value to chronic wound healing for its pro-angiogenic efficacy. © 2013 by the Wound Healing Society.


Yue G.G.-L.,Chinese University of Hong Kong | Yue G.G.-L.,State Key Laboratory of Phytochemistry and Plant Resources in West China | Lee J.K.-M.,Chinese University of Hong Kong | Lee J.K.-M.,State Key Laboratory of Phytochemistry and Plant Resources in West China | And 12 more authors.
Xenobiotica | Year: 2012

Multidrug resistance is a major problem in hepatocellular carcinoma. Hedyotiscone A, a compound isolated from Chinese herbal medicine Hedyotis corymbosa (HC, family Rubiaceae), was used as the chemical marker to distinguish between HC and an anticancer herb Hedyotis diffusa (HD) in our previous study. The present study aimed to investigate whether HA exhibited antiproliferative activities in multidrug-resistant hepatocellular carcinoma cells R-HepG2 and the parental cells HepG2 using MTT assay and [3H]-thymidine incorporation assay. Our results showed that HA could significantly inhibit cell proliferation in R-HepG2 and HepG2 (IC50=43.7 and 56.3 μg/mL, respectively), but not in normal human liver cells WRL-68 (IC50 > 100 μg/mL) cells, suggesting its selective cytotoxic effects. Besides, HA induced apoptosis in R-HepG2 cells, as confirmed by annexin-V & propidium iodide staining, and DNA fragmentation assay. The caspase cascade was activated as shown by a significant increase of cleaved caspases-3, -7 and -9 in HA-treated R-HepG2 cells. The activities and protein expression of P-glycoprotein as well as mRNA expression of MDR1 were also decreased in HA-treated R-HepG2 cells. Our study demonstrated for the first time the antiproliferative activities of hedyotiscone A in multidrug-resistant R-HepG2 cells. The findings revealed the potential of this compound in treating multidrug-resistant tumor. © 2012 Informa UK, Ltd.

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