Zhang Y.,South China University of Technology |
Chen H.,South China University of Technology |
Wen J.-Y.,South China University of Technology |
Wang X.-L.,South China University of Technology |
And 5 more authors.
Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
A new water-soluble iron corrole, 5, 10, 15-tris[4-(carboxy)phenyl]corrolato-iron(III)(FeIIITCPC), was synthesized and characterized. UV-Vis, fluorescence and CD spectra, as well as relative viscosity were used to study the interaction between FeIIITCPC and ct-DNA. Agarose gel electrophrosis was also carried out to investigate the oxidative cleavage ability of FeIIITCPC to pBR322 DNA in the presence of hydrogen peroxide(H2O2) or tert-BuOOH(TBPH). The results suggest that FeIIITCPC binds to CT-DNA via an outside binding mode with intrinsic binding constant Kb of 1.96×105 L·mol-1. In the presence of H2O2 or TBPH, FeIIITCPC exhibits good oxidative cleavage activity toward pBR322 DNA, and TBPH shows a higher oxidative efficiency. Inhibitor tests reveal that neither the hydroxyl radicals nor singlet oxygen is involved in FeIIITCPC mediated DNA oxidative cleavage. It is suggested that (oxo)iron(V) corrole is the possible active intermediate involved in DNA oxidative damage. Source