State Key Laboratory of Crop Genetics and Germplasm Enhancement

Laboratory of, China

State Key Laboratory of Crop Genetics and Germplasm Enhancement

Laboratory of, China

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Wang X.H.,Nanjing Agricultural University | Wang X.H.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Song Y.P.,Nanjing Agricultural University | Song Y.P.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | And 10 more authors.
Journal of Animal and Plant Sciences | Year: 2013

Non-heading Chinese cabbage is one of the main vegetables in Asia, especially in China. To analyze the tolerance of non-heading Chinese cabbage to copper (Cu) stress, 10μM, 100μM and 1mM copper were used to treat two cultivars of 'Wutacai' as well as 'Erqing' and then photosynthesis, chlorophyll fluorescence parameters, biomass and chlorophyll content were investigated. In our study, 1mM copper significantly inhibited the photosynthesis and plant growth of both two cultivars. While at 100μM copper, the growth inhibition was just found in 'Erqing', not in 'Wutacai'. The inhibition of copper on the photosynthesis was not only by stomatal factors but also by non-stomatal factors. Copper stress caused damage of photosystem resulting in reduction of PSII efficiency while non-photochemical-quenching parameter NPQ increased. Besides, at low concentration with 10μM, copper promoted the photosynthesis and plant growth. Therefore, the copper tolerance of non-heading Chinese cabbage was dependent on the genotype, and the plant growth inhibition under copper stress was due to the damage of photosystem. Our results will provide a theoretical basis for a further study on non-heading Chinese cabbage tolerance to copper and will be helpful for the production of non-heading Chinese cabbage in heavy metals contaminated areas.


Shi L.,Nanjing Agricultural University | Qin L.,Nanjing Agricultural University | Xu Y.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Ren A.,Nanjing Agricultural University | And 4 more authors.
Molecular Biology Reports | Year: 2012

This study investigated the role of the mevalonate pyrophosphate decarboxylase gene in the triterpene biosynthetic pathway of Ganoderma lucidum. The mevalonate pyrophosphate decarboxylase gene (mvd) was isolated using a degenerate primer-PCR technique. An analysis of the Gl-mvd transcription profile revealed a positive correlation between the expression of the Gl-mvd gene and triterpene content changes in G. lucidum during development. Furthermore, a promoter deletion analysis was conducted in G. lucidum to investigate the promoter activity and the role of methyl jasmonate (MeJA) responsive elements in the mvd promoter under the MeJA elicitor. The overexpression of Gl-mvd increased triterpene accumulation compared with the wild-type strain and increased the expression of several genes involved in the triterpene biosynthetic pathway. The findings of this study suggest that mvd may play an important role in triterpene biosynthesis regulation. Moreover, there may be the interactions among the genes involved in the triterpene biosynthetic pathway in the G. lucidum. Additionally, this study provides an approach for improving triterpene content through the overexpression of a key gene. © Springer Science+Business Media B.V. 2011.


Sun C.-Q.,Nanjing Agricultural University | Chen F.-D.,Nanjing Agricultural University | Teng N.-J.,Nanjing Agricultural University | Teng N.-J.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | And 4 more authors.
Euphytica | Year: 2010

Chrysanthemum grandiflorum 'Yuhuaxingchen' is an important commercial chrysanthemum cultivar with excellent ornamental quality but low drought tolerance, whereas C. indicum has exceptional drought tolerance. In our earlier study, many hybrid seeds between them were obtained through interspecific hybridization. In the present study, we selected six putative hybrid lines with most drought tolerance from all the hybrid lines by withholding water, indentified their facticity by chromosome counting, and then evaluated their drought tolerance through determining foliar electrolyte leakage (EL), contents of malondialdehyde (MDA) and proline, and plant survival rate after 20% polyethylene glycol 6000 treatment. It was found that 155 out of 282 seeds germinated and only 132 seedlings survived. In addition, chromosome and morphological analysis showed that the six putative hybrids were real hybrids and their morphological features were intermediate between their parents. Furthermore, the density of leaf epidermal hair, proline content, and plant survival rate were the highest in C. indicum and the lowest in C. grandiflorum among the six hybrids and their parents. In contrast, EL value and MDA content were the highest in C. grandiflorum and the lowest in C. indicum. These results suggest that some true hybrids with improved drought tolerance can be obtained through interspecific hybridization in chrysanthemum breeding. Therefore, interspecific hybridization between chrysanthemum cultivars and their wild species may become a promising way to improve their biotic and abiotic resistance in the future breeding. © 2010 Springer Science+Business Media B.V.


Li P.,Nanjing Agricultural University | Han H.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Zhai X.,Jiangsu Animal Husbandry and Veterinary College | He W.,Jiangsu Animal Husbandry and Veterinary College | And 2 more authors.
Journal of Chromatographic Science | Year: 2012

An isocratic reversed-phase high-performance liquid chromatography method with UV detection is developed and validated for the simultaneous determination of ketamine, xylazine, and midazolam in canine plasma. Analytes are extracted from alkalinized samples into diethyl ethermethylene chloride (7:3, v:v) using single-step liquidliquid extraction. Chromatographic separation is performed on a C18 column using a mobile phase containing an acetonitrilemethanol- 10 mM sodium heptanesulfonate buffer adjusted to pH 3, with glacial acetic acid (44:10:46, v:v) at a detection wavelength of 210 nm, with a total runtime of 10 min. The calibration is linear over the range of 78.125-5000 ng/mL for ketamine and 15.625-1000 ng/mL for xylazine and midazolam. The limits of detection are 17.8, 10.3, and 15.1 ng/mL for ketamine, xylazine, and midazolam, respectively. The extraction recoveries are 76.1 for ketamine, 91.0 for midazolam, and 78.2 for xylazine. The method is successfully used for clinical and pharmacokinetic studies of the three-drug fixed dose combination formulations. © 2012 The Author.


Wang Z.,Nanjing Agricultural University | Wang Z.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Wang Z.,Key Laboratory of Southern Vegetable Crop Genetic Improvement | Jiang D.,Nanjing Agricultural University | And 18 more authors.
Gene | Year: 2015

Turnip mosaic virus (TuMV) is the most prevalent viral pathogen infecting most cruciferous plants. MicroRNAs (miRNAs) are around 22 nucleotides long non-protein-coding RNAs that play key regulatory roles in plants. Recent research findings show that miRNAs are involved in plant-virus interaction. However we know little about plant defense and viral offense system networks throughout microRNA regulation pathway. In this study, two small RNA libraries were constructed based on non-heading Chinese cabbage (Brassica campestris ssp. chinensis L. Makino, NHCC) leaves infected by TuMV and healthy leaves, and sequenced using the Illumina-Solexa high-throughput sequencing technology. A total of 86 conserved miRNAs belonging to 25 known miRNA families and 45 novel ones were identified. Among them, twelve conserved and ten new miRNAs were validated by real-time fluorescence quantitative PCR (qPCR). Differential expression analysis showed that 42 miRNAs were down-regulated and 27 miRNAs were up-regulated in response to TuMV stress. A total of 271 target genes were predicted using a bioinformatics approach, these genes are mainly involved in growth and resistance to various stresses. We further selected 13 miRNAs and their corresponding target genes to explore their expression pattern under TuMV and/or cold (4 °C) stresses, and the results indicated that some of the identified miRNAs could link TuMV response with cold response of NHCC. The characterization of these miRNAs could contribute to a better understanding of plant-virus interaction throughout microRNA regulation pathway. This can lead to finding new approach to defend virus infection using miRNA in Chinese cabbage. © 2015.


Wang Z.,Nanjing Agricultural University | Wang Z.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Wang Z.,Key Laboratory of Southern Vegetable Crop Genetic Improvement | Wang F.,Nanjing Agricultural University | And 14 more authors.
Acta Physiologiae Plantarum | Year: 2014

C-repeat binding factor (CBF) signaling pathway is involved in cold acclimation responsive to low temperature and some other stresses. CBF transcription factor family is the key component of this pathway. In this study, eight CBF-like genes, BrCBF1, BrCBF2, BrCBF3, BrCBF4, BrCBF5, and BrCBF6A/B/C were isolated from non-heading Chinese cabbage (Brassicacampestris ssp. chinensis L. Makino, NHCC). The deduced CBF proteins shared high similarity with their Arabidopsis orthologs and localized to the nucleus. Furthermore, quantitative real-time PCR (qPCR) analysis showed that BrCBF1~3 were induced by cold (4 °C) but not drought or abscisic acid (ABA), indicating that they are involved in an ABA-independent pathway; however, BrCBF4~6 were regulated by both drought and ABA, suggesting that they were involved in an ABA-dependent pathway. Nevertheless, unlike Arabidopsis, BrCBF4~6 showed response to both cold and ABA, indicates ABA-independent and ABA-dependent parts of CBF pathway in NHCC might not be completely separate, and these genes may act as the connection points in the network. BrCBFs were also accumulated in response to salicylic acid (SA), methyljasmonate (MeJA), and ethylene (ET), indicating that BrCBF genes might participate in the response to biotic stresses. Taken together, eight CBF genes were isolated from NHCC which compose a functional CBF signaling pathway by participating in response to multiple stresses and performing roles from Arabidopsis to some extent. © 2014, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.


Jiang F.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Jiang F.,Key Laboratory of Southern Vegetable Crop Genetic Improvement | Wang F.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Wang F.,Key Laboratory of Southern Vegetable Crop Genetic Improvement | And 10 more authors.
Plant Molecular Biology Reporter | Year: 2011

Many plants increase in freezing tolerance upon exposure to low non-freezing temperatures, a phenomenon known as cold acclimation. Cold acclimation in Arabidopsis involves rapid cold-induced expression of the inducer of C-repeat/dehydration-responsive element-binding factor (CBF) expression (ICE) transcriptional activators followed by expression of the CBF; subsequently, CBF-targeted genes that increase freezing tolerance. Here, we present evidence for a CBF cold-response pathway in non-heading Chinese cabbage (Brassica campestris ssp. chinensis L. Makino). We show that non-heading Chinese cabbage encodes ICE1-like gene BrICE1 that bracket an open reading frame of 1,491 bp encoding a protein with a potential bHLH domain, which accumulates rapidly in response to low temperature followed closely by expression of the BrCBF gene, an ortholog of the Arabidopsis CBF3-like gene, and then BrCOR14 gene, an ortholog of the Arabidopsis CBF-targeted COR15b gene. An alignment of the later two genes from Arabidopsis, Brassica napus revealed the presence of conserved CANNTG core element and AP2 domain in BrCBF and a CCG core element in BrCOR14. In addition, BrCBF and BrCOR14 showed increased expression induced by low temperature as well as salt and drought, but not by ABA stress which are similar to those of Arabidopsis. We conclude that components of the CBF cold-response pathway are highly conserved in non-heading Chinese cabbage. © 2010 Springer-Verlag.


Peng H.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Li S.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Wang L.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Li Y.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | And 2 more authors.
Physiological and Molecular Plant Pathology | Year: 2013

In order to study the defense response to turnip mosaic virus (TuMV) infection in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino), we cloned the LRR II subfamily genes which comprises six members. They were high homologous to the function-known LRR II genes of Arabidopsis. We investigated their expression through quantitative real-time PCR analysis. TuMV infection induced the expression of these genes locally and systematically, and regulated the endogenous accumulation of salicylic acid (SA). Exogenous SA spraying was able to induce resistance to the susceptibility of the TuMV-infected plants, which might function via inhibiting the viral duplication. Though TuMV-induced SA accumulation was not the determinant in regulating gene expression, it mediated the reaction oxygen species (ROS) burst as a channel of defense. © 2013 Elsevier Ltd.


Xiong A.S.,State Key Laboratory of Crop Genetics and Germplasm Enhancement
BMC genomics | Year: 2014

MicroRNAs (miRNAs) are small, non-coding RNAs of 20 to 24 nucleotides that regulate gene expression and responses to biotic and abiotic stress. Till now, no reports have previously been published concerning miRNAs in celery. Two small RNAs libraries were constructed from two celery varieties, 'Jinnan Shiqin' and 'Ventura', and characterized by deep sequencing. A total of 431 (418 known and 13 novel) and 346 (341 known and five novel) miRNAs were identified in celery varieties 'Jinnan Shiqin' and 'Ventura', respectively. Potential miRNA-target genes were predicted and annotated by screening diverse protein databases, including Gene Ontology, Cluster of Orthologous Groups and Kyoto Encyclopedia of Genes and Genomes. Significant differential expression between the two varieties was seen for 221 miRNAs. qRT-PCR was used to analyze the abundance of six miRNAs under cold and heat stress conditions. The results showed that miRNAs may have important functions in controlling temperature stress in celery. A large number of miRNAs were identified in celery, and their target genes, functional annotations, and gene expression patterns have been explored.These findings provide the first information on celery miRNAs and enhance understanding of celery miRNA regulatory mechanisms under extreme temperature stress.


Jiang Q.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Wang F.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Tan H.-W.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Li M.-Y.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | And 3 more authors.
Molecular Genetics and Genomics | Year: 2015

Oenanthe javanica is an aquatic perennial herb with known medicinal properties and an edible vegetable with high vitamin and mineral content. The understanding of the biology of O. javanica is limited by the absence of information on its genome, transcriptome, and small RNA. In this study, transcriptome sequencing and small RNA sequencing were performed to annotate function genes, develop SSR markers and analyze potential target genes of miRNAs in O. javanica. All reads with total nucleotides number of 1,440,321,408 bp were assembled into 58,072 transcripts and 40,208 unigenes. A total of 1,233 SSRs were identified from O. javanica. Generated unigenes were aligned against seven databases and annotated with functions. A total of 29 potential targets were predicted. Expression of 10 miRNAs and their corresponding target genes under abiotic stresses (heat, cold, salinity, and drought) was validated. All ten miRNAs were confirmed to response to abiotic stresses. A pair of miRNA and its target gene was found. This study can serve as a valuable resource for future studies on O. javanica, which may focus on novel gene discovery, SSR development, gene mapping, and miRNA-affected processes and pathways. This can promote the development of the useful medicinal properties of O. javanica in medical science. © 2014, Springer-Verlag Berlin Heidelberg.

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