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Wang L.,Nanjing Agricultural University | Wang L.,State Key Laboratory of Crop Genetics | Peng H.,Nanjing Agricultural University | Peng H.,State Key Laboratory of Crop Genetics | And 9 more authors.
Plant Biology | Year: 2014

Non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino), an important vegetable crop in China, exhibits a typical sporophytic self-incompatibility (SI) system. To better understand the mechanism of SI response and identify potential candidate proteins involved in the SI system of this vegetable crop, the proteomic approach was taken to identify differential accumulating pistil proteins. Pistils were collected at 0 h and 2 h after self-pollination at anthesis in self-incompatible and compatible lines of non-heading Chinese cabbage, and total proteins were extracted and separated by two-dimensional gel electrophoresis (2-DE). A total of 25 protein spots that displayed differential abundance were identified by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF/TOF MS) and peptide mass fingerprinting (PMF). Among them, 22 protein spots were confidently established. The mRNA levels of the corresponding genes were detected by quantitative RT-PCR. The 22 identified protein spots are involved in energy metabolism (four), protein biosynthesis (three), photosynthesis (six), stress response and defence (five), and protein degradation (four). Among these potential candidate proteins, UDP-sugar pyrophosphorylase could be involved in sucrose degradation to influence pollen germination and growth. Glutathione S-transferases could be involved in pollen maturation, and affect pollen fertility. Senescence-associated cysteine protease, which is related to programmed cell death, could be mainly related to self pollen recognition of non-heading Chinese cabbage. The study will contribute to further investigations of molecular mechanism of sporophytic SI in Brassicaceae. © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands.

Peng H.-T.,State Key Laboratory of Crop Genetics | Wang L.,State Key Laboratory of Crop Genetics | Li Y.,State Key Laboratory of Crop Genetics | Li Y.-X.,State Key Laboratory of Crop Genetics | And 4 more authors.
European Journal of Plant Pathology | Year: 2012

Viral disease, caused by turnip mosaic virus (TuMV), is considered the most destructive disease of non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino). Although several TuMV resistance loci/genes have been mapped or characterized in Brassica vegetables, the mechanism of molecular interaction between TuMV and non-heading Chinese cabbage is poorly understood. Additionally, TuMV response genes need to be identified. The objectives of this study were to identify differentially expressed genes during the incompatible interaction between TuMV and non-heading Chinese cabbage, and validate their expressions. A total of 200 transcript-derived fragments (TDFs) obtained by complementary DNA-amplified fragment length polymorphism were recovered and sequenced. The results revealed that 176 (88.0%) TDFs produced specific sequences, among which 48 (27.3%) sequences were predicted with putative functions using NCBI BLAST. Among the 48 available TDFs, 22 (45.8%) sequences belonging to different functional groups were selected to monitor the changes in their expression in incompatible and compatible interactions by quantitative real-time polymerase chain reaction. To the best of our knowledge, this study provides the first global transcriptomic analysis of non-heading Chinese cabbage genes during an incompatible interaction. The results are expected to aid in characterizing TuMV response genes and clarifying the molecular mechanism of TuMV-host interaction. © 2011 KNPV.

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