State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources

Nanning, China

State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources

Nanning, China
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Shen W.-J.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | Shen W.-J.,South China Agricultural University | Ruan X.-L.,South China Agricultural University | Li X.-S.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | And 5 more authors.
Archives of Virology | Year: 2012

Transgenic rice (Oryza sativa) plants expressing the Pns11 protein of rice gall dwarf virus (RGDV) displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms observed in RGDV-infected rice. Further analysis indicated that the apparent alterations in plant growth and morphology were correlated with the expression levels of microRNA160, microRNA162, microRNA167, microRNA168, and the microRNA target OsARF8. Especially, the striking dwarfing phenotype depended on the high expression level of microRNA167. By analogy to other categories of plant viruses, the RNA silencing suppressors encoded by plant dsRNA viruses function as pathogenicity determinants. These findings significantly deepen our current mechanistic understanding of the RNA silencing suppressor (VSR) encoded by a dsRNA virus and provide additional evidence that interference with microRNA expression is a VSR function utilized by a diverse range of viruses. © 2012 Springer-Verlag.


Si H.-T.,Guangxi University | Si H.-T.,State Key Laboratory of Conservation and Utilization of Subtropical Agro Bioresources | Yu T.-H.,Guangxi University | Yu T.-H.,State Key Laboratory of Conservation and Utilization of Subtropical Agro Bioresources | And 6 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2017

Plants of mangrove forests grow in circumstances with high salinity and high light density, and it is hardly understood the behavior of their stomatal response to the fluctuation of environmental factors and the relationship between their stomata movement and hydraulic function. In this study, nine species of mangrove forests were used to study the response curves of their stomatal conductance (Gs) to different transient light densities and vapor pressure deficit (VPD), diurnal variation of stomatal conductance, predawn water potential (Ψpd), midday water potential (Ψmin) and leaf hydraulic conductance (Kleaf). The results showed that the stomatal conductance increased linearly with the increase in light intensity, and the sensitivity of stomatal response to light was correlated with Ψpd. Moreover, with the increase in transient VPD, the nine species displayed three different modes of stomatal response: (1) continuously increasing Gs; (2) increasing Gs at rather low VPD and then decreasing Gs; (3) decreasing Gs at low VPD and then remaining a low Gs with further increasing of VPD. These different stomatal response modes might be related to leaf anatomical structure and water use strategies. In addition, we also found that Gmax was significantly linearly correlated with Ψpd and Ψmin. This indicates that higher water potential status could help plants to achieve higher stomatal opening. Moreover, we also found that there was significant positive correlation between Kleaf and Gmax, indicating the coordination between stomatal behavior and hydraulic function in mangrove plants. © 2017, Science Press. All right reserved.


Wu G.-Z.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | Wu G.-Z.,Ministry of Agriculture Key Laboratory of Tropical Agro environment | Wu G.-Z.,South China Agricultural University | Hu L.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | And 13 more authors.
Chinese Journal of Applied Ecology | Year: 2012

In a long history of interactions between insects and plants, plants have developed various anti-insect compounds and defense signaling transduction pathways to defend against herbivorous insects, while insects have responded with sophisticated detoxification enzyme systems to protect against the toxicity of anti-insect compounds. In this study, the 2nd or 3rd instar of Spodoptera litura larvae were successively fed with the diets containing 0.5% soybean trypsinase inhibitor(SBTI) for six generations to evaluate the effects of SBTI and defense signaling compounds on the activities of detoxification enzymes carboxylesterase(CarE) and glutathione-S-transferase(GST) in the midgut and fatbody of the larvae. After fed with the diets, the CarE and GST activities in the 5th instar larvae increased significantly. The CarE activity in the midgut and fatbody of the second generation larvae was the highest, being 2.06 and 2.40 times, and 1.96 and 2.70 times of that of the control, and the GST activity in the midgut and fatbody of the fourth and second generations was the highest, being 7.03 and 11.58 times, and 5.71 and 3.60 times of that of the control, respectively. These induced enzyme activities decreased gradually when the larvae continuously grew with the SBTI-containing diets. In addition, when the S. litura larvae were pre-exposed to methyl jasmonate (MeJA) or methyl salicylate(MeSA) for 48 h or fed with the diets containing 0.5% SBTI, the activities of CarE and GST in the midgut and fatbody increased significantly, and, when the 2nd instar larvae were pre-exposed to MeJA and MeSA for 48 h, the effects of SBTI on the GST activity in larval midgut and fatbody were reduced.


Song Y.-Y.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | Song Y.-Y.,Ministry of Agriculture Key Laboratory of Tropical Agro environment | Song Y.-Y.,South China Agricultural University | Huang K.,South China Agricultural University | And 7 more authors.
Chinese Journal of Applied Ecology | Year: 2012

Streptomyces can produce an overwhelming majority of known antibiotics and several biologically active compounds, but whether Streptomyces can display allelopathic effects on higher plants is largely unknown. In this study, seven actinomyces strains isolated from soils showed inhibitory effect on plant seedlings growth, among which, Streptomyces sp. 6803 had strong capability in inhibiting the seedlings growth of Brassica campestris and Echinochloa crusgalli in both solid and liquid cultures. The dilute solution (× 5) of fermented broth inhibited the seedlings growth of B. campestris and E. crusgalli by 60.7% and 61.3%, respectively. Based on the morphological and physiological-biochemical characteristics and 16S rRNA sequencing, Streptomyces sp. 6803 was identified as Streptomyces arenae, with the 16S rRNA sequence identity being 99.28%. Ultraviolet radiation and diethyl sulfate (DES) were used to produce mutants to enhance the allelopathic potential of this strain. After 80 and 100 seconds of ultraviolet radiation, the dilute solution (× 10) of fermented broth of obtained mutants UV8024 and UV100-2 showed 37.5% and 38.1% higher inhibition effect on the root growth of B. campestris seedlings, respectively, compared with the control. The mutant D507 obtained through 1% DES treatment for 50 min showed 29.8% higher inhibition effect on the root growth of B. campestris seedlings. This study showed that Streptomyces sp. 6803 had allelopathic effect on higher plants, and it was possible to enhance the allelopathic potential of the strain via mutation breeding.


Hu M.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | Hu M.,Guangxi University | Su D.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | Su D.,Guangxi University | And 5 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2012

A micellar electrokinetic chromatography-mass spectrometric method based on laurel acyl malic acid ester (LMAE) for the separation and determination of coptisine, berberine, jatrorrhizine, phellodendrine and ligustrazine in Niuhuang Shangqing Tablets was established. The baseline separation of the five compounds was attained within 18 min by an uncoated capillary (88 cm ×50 μm) on the operating voltage of 25 kV using 7. 5 mmol / L LMAE-15 mmol / L ammonia-50 mmol / L ammonium acetate mixture (pH =7. 0) containing 12. 5% (v / v) acetonitrile as the electrophoretic medium and 50% 2-propanol aqueous solution (containing 3 mmol / L acetic acid) as the sheath liquid. The peak area of each component to its concentration showed a good linear relationship. The relative standard deviations of migration times and peak areas of the five components were less than 5% and the recoveries were between 96. 0% and 105%. The developed method is simple, rapid, accurate and is suitable for the routine analysis of the five alkaloid components in Niuhuang Shangqing Tablets.


Li X.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | Li X.,South China Agricultural University | Bai T.,State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources | Bai T.,South China Agricultural University | And 5 more authors.
Proteome Science | Year: 2013

Background: Fusarium wilt of banana is one of the most destructive diseases in the world. This disease has caused heavy losses in major banana production areas. Except for molecular breeding methods based on plant defense mechanisms, effective methods to control the disease are still lacking. Dynamic changes in defense mechanisms between susceptible, moderately resistant, and highly resistant banana and Fusarium oxysporum f. sp. cubense tropical race 4 (Foc4) at the protein level remain unknown. This research reports the proteomic profile of three banana cultivars in response to Foc4 and transcriptional levels correlated with their sequences for the design of disease control strategies by molecular breeding.Results: Thirty-eight differentially expressed proteins were identified to function in cell metabolism. Most of these proteins were positively regulated after Foc4 inoculation. These differentially regulated proteins were found to have important functions in banana defense response. Functional categories implicated that these proteins were associated with pathogenesis-related (PR) response; isoflavonoid, flavonoid, and anthocyanin syntheses; cell wall strengthening; cell polarization; reactive oxygen species production and scavenging; jasmonic acid-, abscisic acid-, and auxin-mediated signaling conduction; molecular chaperones; energy; and primary metabolism. By comparing the protein profiles of resistant and susceptible banana cultivars, many proteins showed obvious distinction in their defense mechanism functions. PR proteins in susceptible 'Brazil' were mainly involved in defense. The proteins related to PR response, cell wall strengthening and antifungal compound synthesis in moderately resistant 'Nongke No.1' were mainly involved in defense. The proteins related to PR response, cell wall strengthening, and antifungal compound synthesis in highly resistant 'Yueyoukang I' were mainly involved in defense. 12 differentially regulated genes were selected to validate through quantitative real time PCR method. Quantitative RT-PCR analyses of these selected genes corroborate with their respective protein abundance after pathogen infection.Conclusions: This report is the first to use proteomic profiling to study the molecular mechanism of banana roots infected with Foc4. The differentially regulated proteins involved in different defense pathways are likely associated with different resistant levels of the three banana cultivars. © 2013 Li et al.; licensee BioMed Central Ltd.


PubMed | State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources
Type: Journal Article | Journal: Ying yong sheng tai xue bao = The journal of applied ecology | Year: 2013

Streptomyces can produce an overwhelming majority of known antibiotics and several biologically active compounds, but whether Streptomyces can display allelopathic effects on higher plants is largely unknown. In this study, seven actinomyces strains isolated from soils showed inhibitory effect on plant seedlings growth, among which, Streptomyces sp. 6803 had strong capability in inhibiting the seedlings growth of Brassica campestris and Echinochloa crusgalli in both solid and liquid cultures. The dilute solution (x 5) of fermented broth inhibited the seedlings growth of B. campestris and E. crusgalli by 60.7% and 61.3%, respectively. Based on the morphological and physiological-biochemical characteristics and 16S rRNA sequencing, Streptomyces sp. 6803 was identified as Streptomyces arenae, with the 16S rRNA sequence identity being 99.28%. Ultraviolet radiation and diethyl sulfate (DES) were used to produce mutants to enhance the allelopathic potential of this strain. After 80 and 100 seconds of ultraviolet radiation, the dilute solution (x10) of fermented broth of obtained mutants UV8024 and UV100-2 showed 37.5% and 38.1% higher inhibition effect on the root growth of B. campestris seedlings, respectively, compared with the control. The mutant D507 obtained through 1% DES treatment for 50 min showed 29.8% higher inhibition effect on the root growth of B. campestris seedlings. This study showed that Streptomyces sp. 6803 had allelopathic effect on higher plants, and it was possible to enhance the allelopathic potential of the strain via mutation breeding.


PubMed | State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources
Type: Journal Article | Journal: Proteome science | Year: 2013

Fusarium wilt of banana is one of the most destructive diseases in the world. This disease has caused heavy losses in major banana production areas. Except for molecular breeding methods based on plant defense mechanisms, effective methods to control the disease are still lacking. Dynamic changes in defense mechanisms between susceptible, moderately resistant, and highly resistant banana and Fusarium oxysporum f. sp. cubense tropical race 4 (Foc4) at the protein level remain unknown. This research reports the proteomic profile of three banana cultivars in response to Foc4 and transcriptional levels correlated with their sequences for the design of disease control strategies by molecular breeding.Thirty-eight differentially expressed proteins were identified to function in cell metabolism. Most of these proteins were positively regulated after Foc4 inoculation. These differentially regulated proteins were found to have important functions in banana defense response. Functional categories implicated that these proteins were associated with pathogenesis-related (PR) response; isoflavonoid, flavonoid, and anthocyanin syntheses; cell wall strengthening; cell polarization; reactive oxygen species production and scavenging; jasmonic acid-, abscisic acid-, and auxin-mediated signaling conduction; molecular chaperones; energy; and primary metabolism. By comparing the protein profiles of resistant and susceptible banana cultivars, many proteins showed obvious distinction in their defense mechanism functions. PR proteins in susceptible Brazil were mainly involved in defense. The proteins related to PR response, cell wall strengthening and antifungal compound synthesis in moderately resistant Nongke No.1 were mainly involved in defense. The proteins related to PR response, cell wall strengthening, and antifungal compound synthesis in highly resistant Yueyoukang I were mainly involved in defense. 12 differentially regulated genes were selected to validate through quantitative real time PCR method. Quantitative RT-PCR analyses of these selected genes corroborate with their respective protein abundance after pathogen infection.This report is the first to use proteomic profiling to study the molecular mechanism of banana roots infected with Foc4. The differentially regulated proteins involved in different defense pathways are likely associated with different resistant levels of the three banana cultivars.


PubMed | State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources
Type: Journal Article | Journal: Archives of virology | Year: 2012

Transgenic rice (Oryza sativa) plants expressing the Pns11 protein of rice gall dwarf virus (RGDV) displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms observed in RGDV-infected rice. Further analysis indicated that the apparent alterations in plant growth and morphology were correlated with the expression levels of microRNA160, microRNA162, microRNA167, microRNA168, and the microRNA target OsARF8. Especially, the striking dwarfing phenotype depended on the high expression level of microRNA167. By analogy to other categories of plant viruses, the RNA silencing suppressors encoded by plant dsRNA viruses function as pathogenicity determinants. These findings significantly deepen our current mechanistic understanding of the RNA silencing suppressor (VSR) encoded by a dsRNA virus and provide additional evidence that interference with microRNA expression is a VSR function utilized by a diverse range of viruses.


PubMed | State Key Laboratory of Conservation and Utilization of Subtropical Agro bioresources
Type: Journal Article | Journal: Se pu = Chinese journal of chromatography | Year: 2013

A micellar electrokinetic chromatography-mass spectrometric method based on laurel acyl malic acid ester (LMAE) for the separation and determination of coptisine, berberine, jatrorrhizine, phellodendrine and ligustrazine in Niuhuang Shangqing Tablets was established. The baseline separation of the five compounds was attained within 18 min by an uncoated capillary (88 cm x 50 microm) on the operating voltage of 25 kV using 7.5 mmol/L LMAE-15 mmol/L ammonia-50 mmol/L ammonium acetate mixture (pH = 7.0) containing 12.5% (v/v) acetonitrile as the electrophoretic medium and 50% 2-propanol aqueous solution (containing 3 mmol/L acetic acid) as the sheath liquid. The peak area of each component to its concentration showed a good linear relationship. The relative standard deviations of migration times and peak areas of the five components were less than 5% and the recoveries were between 96.0% and 105%. The developed method is simple, rapid, accurate and is suitable for the routine analysis of the five alkaloid components in Niuhuang Shangqing Tablets.

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