State Key Laboratory of Chemo Biosensing and Chemometrics

Changsha, China

State Key Laboratory of Chemo Biosensing and Chemometrics

Changsha, China

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Chen H.,JiShou University | Chen H.,State Key Laboratory of Chemo Biosensing and Chemometrics | Zhang Z.,JiShou University | Zhang Z.,State Key Laboratory of Chemo Biosensing and Chemometrics | And 3 more authors.
Electrochimica Acta | Year: 2014

In this work, a novel imprinted electrochemical sensor based on nickel nanoparticles-graphene modified electrode carbon electrode was developed for the determination of 3, 3', 5, 5'-tetrabromobisphenol A (TBBPA). The preparation procedure of imprinted electrode was discussed. The electrochemical characteristics of the imprinted sensor were investigated using cyclic voltammetry and electrochemical impedance spectroscopy in detail. The response currents density of the imprinted electrode exhibited a linear relationship toward TBBPA concentrations ranging from 5.0 × 10-10 to 1.0 × 10-5 mol L-1 with the detection limit of 1.3 × 0-10 mol L-1 (S/N = 3). The developed electrochemical imprinted sensor was applied to the direct determination of TBBPA in tap water, rain and lake water samples using standard addition method successfully. © 2013 Published by Elsevier Ltd.


Chen H.-J.,JiShou University | Zhang Z.-H.,JiShou University | Zhang Z.-H.,State Key Laboratory of Chemo Biosensing and Chemometrics | Luo L.-J.,JiShou University | Yao S.-Z.,State Key Laboratory of Chemo Biosensing and Chemometrics
Sensors and Actuators, B: Chemical | Year: 2012

A surface-imprinting biosensor was prepared for bovine serum albumin (BSA) detection using chitosan-coated magnetic nanoparticles modified multi-walled carbon nanotubes (MNPs/CS-MWNTs) as a signal amplifier. The property of the MNPs/CS-MWNTs was studied using infrared spectra and their configurations were characterized with scanning electron microscopy. The molecularly imprinted biosensor was tested by differential pulse voltammetry to investigate the relationship between the response current and BSA concentration. The results showed that a wide detection linear range (1.0 × 10 -4-1.0 × 10 -10 g mL -1) for the determination of bovine serum albumin with the low detection limit of 2.8 × 10 -11 g mL -1 for S/N = 3 was obtained. The surface imprinted biosensor exhibited excellent selectivity, high sensitivity, and good reproducibility. The proposed biosensor also exhibited fast balance response time of 30 s, which is propitious to rapid detect protein in spot specimens. The merits of the imprinted biosensor suggested an attractive and broadly applicable way for developing biosensors. © 2012 Elsevier B.V. All rights reserved.


Zhang Z.,JiShou University | Zhang Z.,State Key Laboratory of Chemo Biosensing and Chemometrics | Hu Y.,JiShou University | Zhang H.,JiShou University | And 3 more authors.
Biosensors and Bioelectronics | Year: 2010

A novel sensitive and selective imprinted electrochemical sensor was successfully constructed for the direct detection of l-histidine by combination of a molecular imprinting film and multi-walled carbon nanotubes (MWNTs). The sensor was fabricated onto an indium tin oxide (ITO) electrode via stepwise modification of MWNTs and a thin film of molecularly imprinted polymers (MIPs) via sol-gel technology. The introduced MWNTs exhibited noticeable enhancement on the sensitivity of the MIPs sensor. Meanwhile, the molecularly imprinted film displayed high sensitivity and excellent selectivity for the target molecule l-histidine. The proposed imprinted sensor was characterized by using scanning electron microscope (SEM) and electrochemical methods involving cyclic voltammetry (CV), differential pulse voltammetry (DPV) and amperometric i-t curve. A linear ranging from 2.0μmolL-1 to 1.0mmolL-1 for the detection of l-histidine was observed with the detection limit of 5.8×10-9molL-1 for S/N=3. This imprinted electrochemical sensor was successfully employed to detect l-histidine in human blood serum. © 2010 Elsevier B.V.


Zhang Z.-h.,JiShou University | Zhang Z.-h.,State Key Laboratory of Chemo Biosensing and Chemometrics | Hu Y.-f.,JiShou University | Zhang H.-b.,JiShou University | And 2 more authors.
Journal of Electroanalytical Chemistry | Year: 2010

A novel electrochemical sol-gel imprinted sensor for sensitive and convenient determination of thymidine was developed. Thin film of molecularly imprinted sol-gel polymers with specific binding sites for thymidine was cast on carbon electrode by electrochemical deposition. Multi-walled carbon nanotubes (MWCNTs) were introduced for the enhancement of electronic transmission and sensitivity. The morphology and performance of the imprinted film was characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV) and amperometric measurements (i-t) in detail. The results showed that the imprinted film exhibited high selectivity toward thymidine. The linear range is over the range from 2 to 22 μmol L-1, and the linear regression equation for thymidine is I = 0.867C + 0.232 with the detection limit of 1.6 × 10-9 mol L-1(S/N = 3). The imprinted sensor was successfully employed to detect thymidine in some zidovudine-tablet samples. © 2010 Elsevier B.V. All rights reserved.


Zhang Z.,JiShou University | Zhang Z.,State Key Laboratory of Chemo Biosensing and Chemometrics | Cai R.,JiShou University | Long F.,JiShou University | Wang J.,JiShou University
Talanta | Year: 2015

A novel imprinted electrochemical sensor based on graphene/carbon nanotubes three-dimensional (3D) nanocomposites modified carbon electrode was developed for the determination of tetrabromobisphenol A. The imprinted film was prepared by one-step electrodeposition technique with pyrrole as the functional monomer and tetrabromobisphenol A as the template molecule. The imprinted sensor was used for the determination of tetrabromobisphenol A with differential pulse voltammetry. A linear relationship between the response currents and the negative logarithm of tetrabromobisphenol A concentrations was obtained in the concentrations range of 1.0 × 10-11-1.0 × 10-8 mol L-1 with a detection limit of 3.7 × 10-12 mol L-1 (S/N=3). The proposed imprinted sensor showed excellent selectivity towards tetrabromobisphenol A. With good reproducibility and stability, the imprinted electrochemical sensor was used to detect tetrabromobisphenol A in fish samples successfully with the recoveries of 93.3-107.7%. © 2014 Elsevier B.V. All rights reserved.


Zhang Z.,JiShou University | Zhang Z.,State Key Laboratory of Chemo Biosensing and Chemometrics | Hu Y.,JiShou University | Zhang H.,JiShou University | And 2 more authors.
Journal of Colloid and Interface Science | Year: 2010

A novel sensitive molecularly imprinted electrochemical sensor was constructed for selective detection of clindamycin by combination of a multi-wall carbon nanotube (MWNT) layer with a thin molecularly imprinted sol-gel film. The sensor was fabricated onto Au electrode via stepwise modification of MWNT and a thin sol-gel film of molecularly imprinted polymers (MIP) by using electrodeposition method. The molecularly imprinted film displayed excellent selectivity towards clindamycin. Due to such combination, the sensor responded quickly to clindamycin. The response peak current was linear to the concentration of clindamycin in the range from 5.0 × 10-7 mol L-1 to 8.0 × 10-5 mol L-1, and the detection limit was 2.44 × 10-8 mol L-1. This imprinted sensor was applied to the determination of clindamycin in human urine samples successfully. These results revealed that the imprinted sensor fulfilled the selectivity, sensitivity, speed and simplicity requirements for clindamycin detection, and provided possibilities of clinical application in physiological fluids. © 2009 Elsevier Inc. All rights reserved.


Chen H.-J.,JiShou University | Zhang Z.-H.,JiShou University | Cai R.,JiShou University | Kong X.-Q.,JiShou University | And 3 more authors.
Analyst | Year: 2013

A simple strategy for the indirect detection of 3,3′,5,5′- tetrabromobisphenol A (TBBPA) was developed with a surface imprinted sensor based on an electrochemical reduced graphene modified carbon electrode. The preparation procedure of imprinted electrode and the response mechanism of the imprinted electrode toward TBBPA are discussed in detail. The electrochemical characteristics of the imprinted sensor were investigated using cyclic voltammetry and their morphologies were characterized using scanning electron microscopy. The indirect detection for TBBPA was successfully implemented by monitoring the peak current of Fe(CN)63-/4--TBBPA complex. The response currents of the imprinted sensor exhibited a linear relationship toward the TBBPA concentration range from 0.5 to 4.5 nM with a detection limit of 0.23 nM (S/N = 3). The fabricated electrochemical imprinted sensor was successfully applied to the detection of TBBPA in rain and lake water samples using the standard addition method. With the advantages of high sensitivity and simple operation, the determination methodology is a promising candidate for TBBPA detection in real water samples. This journal is © 2013 The Royal Society of Chemistry.


Hu Y.-F.,JiShou University | Zhang Z.-H.,JiShou University | Zhang Z.-H.,State Key Laboratory of Chemo Biosensing and Chemometrics | Zhang H.-B.,JiShou University | And 2 more authors.
Talanta | Year: 2011

A sensitive and selective electrochemical sensor based on a polyaniline modified carbon electrode for the determination of l-phenylalanine has been proposed by utilizing β-cyclodextrin (β-CD) incorporated multi-walled carbon nanotube (MWNT) and imprinted sol-gel film. The electrochemical behavior of the sensor towards l-phenylalanine was investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and amperometric i-t curve. The surface morphologies of layer-by-layer assembly electrodes were displayed by scanning electron microscope (SEM). The response mechanism of the imprinted sensor for l-phenylalanine was based on the inclusion interaction of β-CD and molecular recognition capacity of the imprinted film for l-phenylalanine. A linear calibration plot was obtained covering the concentration range from 5.0 × 10 -7 to 1.0 × 10 -4 mol L -1 with a detection limit of 1.0 × 10 -9 mol L -1. With excellent sensitivity, selectivity, stability, reproducibility and recovery, the electrochemical imprinted sensor was used to detect l-phenylalanine in blood plasma samples successfully. © 2011 Elsevier B.V. All rights reserved.


Zhang Z.,JiShou University | Zhang Z.,State Key Laboratory of Chemo Biosensing and Chemometrics | Luo L.,JiShou University | Cai R.,JiShou University | Chen H.,JiShou University
Biosensors and Bioelectronics | Year: 2013

A highly sensitive and selective molecularly imprinted (MIP) sensor combined with magnetic molecularly imprinted solid phase extraction (MMISPE) was developed for the determination of dibutyl phthalate (DBP) in complex matrixes. The magnetic molecularly imprinted polymer (MMIP) was synthesized as solid phase extraction (SPE) sorbet to extract DBP from complex matrixes and as sensing element to improve the selectivity of the imprinted sensor. The morphologies of MMIP and MIP-sensor were characterized by using scanning electron microscope (SEM) and transmission electron microscopy (TEM). The electrochemical performances of MIP-sensor were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The conditions of preconcentration, elution and electrochemical determination were studied in detail. Under the optimized experimental conditions, the response currents of the MIP-sensor exhibited a linear relationship towards DBP concentrations ranging from 1.0×10-8g/L to 1.0×10-3g/L. The limit of detection of the MMIP-sensor coupled with the MMISPE was calculated as 0.052ng/L. The MMIP-sensor coupled with the MMISPE was applied to detect DBP in complex samples successfully. © 2013 Elsevier B.V.


Hu Y.,JiShou University | Zhang Z.,JiShou University | Zhang Z.,State Key Laboratory of Chemo Biosensing and Chemometrics | Zhang H.,JiShou University | And 2 more authors.
Journal of Solid State Electrochemistry | Year: 2012

A sensitive molecularly imprinted electrochemical sensor was developed for selective detection of streptomycin by combination of mercaptoacetic acid-modified PbS nanoparticles with Au-coated Fe 3O 4 magnetic nanoparticles dispersed multi-walled carbon nanotubes doped chitosan film. The imprinted sensor was fabricated onto the Au electrode via stepwise modification of nanocomposites and an electrodeposited thin film of molecularly imprinted polymers via sol-gel technology. The morphologies and electrochemical behaviors of the imprinted sensor were characterized by scanning electron microscope, cyclic voltammetry, and differential pulse voltammetry, respectively. The prepared sensor showed very high recognition ability and selectivity for streptomycin. Under optimal conditions, the imprinted sensor displayed good electrocatalytic activity to the redox of streptomycin. And the differential voltammetric anodic peak current was linear to the logarithm of streptomycin concentration in the range from 1.0×10 -6 to 1.0×10 -3 mol L -1, and the detection limit obtained was 1.5×10 -9 mol L -1. This proposed imprinted sensor was used successfully for streptomycin determination in different injection solution samples. © Springer-Verlag 2011.

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