State Key Laboratory of Applied Microbiology Southern China

Guangzhou, China

State Key Laboratory of Applied Microbiology Southern China

Guangzhou, China
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Yang Y.,Guangdong Institute of Microbiology | Kong G.,State Key Laboratory of Applied Microbiology Southern China | Chen X.,Guangdong Institute of Microbiology | Lian Y.,Guangdong Institute of Microbiology | And 3 more authors.
Frontiers in Microbiology | Year: 2017

Bacterial extracellular electron transfer (EET) plays a key role in various natural and engineering processes. Outer membrane c-type cytochromes (OMCs) are considered to be essential in bacterial EET. However, most bacteria do not have OMCs but have redox proteins other than OMCs in their extracellular polymeric substances of biofilms. We hypothesized that these extracellular non-cytochrome c proteins (ENCP) could contribute to EET, especially with the facilitation of electron mediators. This study compared the electrode respiring capacity of wild type Shewanella decolorationis S12 and an OMC-deficient mutant. Although the OMC-deficient mutant was incapable in direct electricity generation in normal cultivation, it regained electricity generation capacity (26% of the wide type) with the aid of extracellular electron mediator (riboflavin). Further bioelectrochemistry and X-ray photoelectron spectroscopy analysis suggested that the ENCP, such as proteins with Fe-S cluster, may participate in the falvin-mediated EET. The results highlighted an important and direct role of the ENCP, generated by either electricigens or other microbes, in natural microbial EET process with the facilitation of electron mediators. © 2017 Yang, Kong, Chen, Lian, Liu and Xu.


Wu L.,Hefei Normal University | Sun Q.,Anhui University | Ni J.,Guangdong Institute of Microbiology | Ni J.,State Key Laboratory of Applied Microbiology Southern China
Applied Ecology and Environmental Research | Year: 2017

The role of rare biosphere in maintaining the community diversity and metabolic activity has recently been highlighted. However, it is still unclear whether the rare species play the same role in maintaining the community diversity. Considering different responses of microbial species on environment changes, we speculate rare species played different roles in maintaining the α-diversity. To verify the speculation, we analyzed the prokaryotic species in three eutrophic rivers and one eutrophic lake via Miseq sequencing of 16S rDNA amplicons. Although more than 50 phyla were identified from 20 samples, only seven were dominant. The dominant species were strictly restricted. The rare operational taxonomic units (OTUs) belonging to the dominant phyla played a crucial role in maintaining the community diversity. These results intensified our knowledge on the role of rare species in maintaining the diversity of microbial community. © 2017, ALÖKI Kft., Budapest, Hungary.


Yang Y.,Guangdong Institute of Microbiology | Yang Y.,State Key Laboratory of Applied Microbiology Southern China | Lu Z.,China Institute of Technology | Xia C.,State Key Laboratory of Applied Microbiology Southern China | And 5 more authors.
Bioresource Technology | Year: 2015

To test the long-term applicability of scaled-up sediment microbial fuel cells (SMFCs) in simultaneous bioremediation of toxic-contaminated sediments and power-supply for electronic devices, a 100. L SMFC inoculate with heavily contaminated sediments has been assembled and operated for over 2. years without external electron donor addition. The total organic chemical (TOC) degradation efficiency was 22.1% in the electricity generating SMFCs, which is significantly higher than that in the open-circuited SMFC (3.8%). The organic matters including contaminants in the contaminated sediments were sufficient for the electricity generation of SMFCs, even up to 8.5. years by the present SMFC theoretically. By using a power management system (PMS), the SMFC electricity could be harvested into batteries and used by commercial electronic devices. The results indicated that the SMFC-PMS system could be applied as a long-term and effective tool to simultaneously stimulate the bioremediation of the contaminated sediments and supply power for commercial devices. © 2014 Elsevier Ltd.


Feng J.,State Key Laboratory of Applied Microbiology Southern China | Chen J.,Guangdong Demay Biotechnology Co. | Chen M.,State Key Laboratory of Applied Microbiology Southern China | Su X.,State Key Laboratory of Applied Microbiology Southern China | Shi Q.,State Key Laboratory of Applied Microbiology Southern China
Journal of Applied Polymer Science | Year: 2017

The main objective of this study was to investigate the algal and fungal resistance of biocide-treated wood flour (WF)/high density polyethylene (HDPE) and bamboo flour (BF)/HDPE composites. The biocides included 4,5-dichloro-2-octyl-isothiazolone (DCOIT), zinc pyrithione (ZPT), and carbendazim (MBC). Resistance to algae and fungi was evaluated by artificially accelerated tests. Treated and untreated samples were exposed to algae (Chlorella vulgaris, Ulothrix sp., Scenedesmus quadricauda, and Oscillatoria sp.) and fungi (Coriolus versicolor and Poria placenta) for 21 days and 12 weeks, respectively. The volatile components of WF and BF extractives were analyzed by gas chromatography-mass spectrometry (GC-MS). The results indicated that incorporation of DCOIT, ZPT, and MBC effectively enhanced the durability of WF/HDPE and BF/HDPE composites against algal and fungal decay. Accordingly, DCOIT, ZPT, and MBC can be used as potential biocides for both WF/HDPE and BF/HDPE composites. GC-MS analysis suggested that palmitic acid, oleic acid, stigmasta-3, 5-dien-7-one, and vanillin in WF possibly provided some resistance to fungal attack, whereas di (2-ethylhexyl) phthalate and linoleic acid in BF were responsible for algal resistance. © 2017 Wiley Periodicals, Inc.


Yang X.,Guangdong Institute of Microbiology | Yang X.,State Key Laboratory of Applied Microbiology Southern China | Yu L.,Dalhousie University | Chen Z.,Guangdong Institute of Microbiology | And 3 more authors.
Scientific Reports | Year: 2016

Traditional risk assessment and source apportionment of sediments based on bulk polycyclic aromatic hydrocarbons (PAHs) can introduce biases due to unknown aging effects in various sediments. We used a mild solvent (hydroxypropyl-β-cyclodextrin) to extract the bioavailable fraction of PAHs (a-PAHs) from sediment samples collected in Pearl River, southern China. We investigated the potential application of this technique for ecological risk assessments and source apportionment. We found that the distribution of PAHs was associated with human activities and that the a-PAHs accounted for a wide range (4.7%-21.2%) of total-PAHs (t-PAHs), and high risk sites were associated with lower t-PAHs but higher a-PAHs. The correlation between a-PAHs and the sediment toxicity assessed using tubificid worms (r = -0.654, P = 0.021) was greater than that from t-PAH-based risk assessment (r = -0.230, P = 0.472). Moreover, the insignificant correlation between a-PAH content and mPEC-Q of low molecular weight PAHs implied the potiential bias of t-PAH-based risk assessment. The source apportionment from mild extracted fractions was consistent across different indicators and was in accordance with typical pollution sources. Our results suggested that mild extraction-based approaches reduce the potential error from aging effects because the mild extracted PAHs provide a more direct indicator of bioavailability and fresher fractions in sediments.


Yang Y.,Guangdong Institute of Microbiology | Yang Y.,State Key Laboratory of Applied Microbiology Southern China | Xiang Y.,State Key Laboratory of Applied Microbiology Southern China | Xu M.,Guangdong Institute of Microbiology | Xu M.,State Key Laboratory of Applied Microbiology Southern China
Scientific Reports | Year: 2015

Viability is a common issue of concern in almost all microbial processes. Fluorescence-based assays are extensively used in microbial viability assessment, especially for mixed-species samples or biofilms. Propidium iodide (PI) is the most frequently used fluorescence indicator for cell viability based on the membrane permeability. Our results showed that the accumulation of succinate from fumarate respiration could induce PI-permeability in Shewanella decolorationis biofilm cells. Confocal laser scanning microscope further showed that the PI-permeable membrane could be repaired in situ when the extracellular succinate was eliminated by switching fumarate respiration to electrode respiration. Simultaneously with the membrane repair, the electrode respiring capacity of the originally PI-permeable cells was recovered. Agar-colony counts suggested that a major portion of the repaired cells were viable but nonculturable (VBNC). The results evidenced that S. decolorationis S12 has the capacity to repair PI-permeable membranes which suggests a reevaluation of the fate and function of the PI-permeable bacteria and expanded our knowledge on the flexibility of bacterial survival status in harsh environments.


Yang Y.,State Key Laboratory of Applied Microbiology Southern China | Yang Y.,Guangdong Institute of Microbiology | Xiang Y.,Guangdong Institute of Microbiology | Xia C.,Guangdong Institute of Microbiology | And 5 more authors.
Bioresource Technology | Year: 2014

To understand the interactions between bacterial electrode respiration and the other ambient bacterial electron acceptor reductions, alternative electron acceptors (nitrate, Fe2O3, fumarate, azo dye MB17) were added singly or multiply into Shewanella decolorationis microbial fuel cells (MFCs). All the added electron acceptors were reduced simultaneously with current generation. Adding nitrate or MB17 resulted in more rapid cell growth, higher flavin concentration and higher biofilm metabolic viability, but lower columbic efficiency (CE) and normalized energy recovery (NER) while the CE and NER were enhanced by Fe2O3 or fumarate. The added electron acceptors also significantly influenced the cyclic voltammetry profile of anode biofilm probably via altering the cytochrome c expression. The highest power density was observed in MFCs added with MB17 due to the electron shuttle role of the naphthols from MB17 reduction. The results provided important information for MFCs applied in practical environments where contains various electron acceptors. © 2014 Elsevier Ltd.


Xu M.,Guangdong Institute of Microbiology | Xu M.,State Key Laboratory of Applied Microbiology Southern China | Zhang Q.,Guangdong Institute of Microbiology | Zhang Q.,Guilin University of Technology | And 10 more authors.
ISME Journal | Year: 2014

Nitrate is an important nutrient and electron acceptor for microorganisms, having a key role in nitrogen (N) cycling and electron transfer in anoxic sediments. High-nitrate inputs into sediments could have a significant effect on N cycling and its associated microbial processes. However, few studies have been focused on the effect of nitrate addition on the functional diversity, composition, structure and dynamics of sediment microbial communities in contaminated aquatic ecosystems with persistent organic pollutants (POPs). Here we analyzed sediment microbial communities from a field-scale in situ bioremediation site, a creek in Pearl River Delta containing a variety of contaminants including polybrominated diphenyl ethers (PBDEs) and polycyclic aromatic hydrocarbons (PAHs), before and after nitrate injection using a comprehensive functional gene array (GeoChip 4.0). Our results showed that the sediment microbial community functional composition and structure were markedly altered, and that functional genes involved in N-, carbon (C)-, sulfur (S)-and phosphorus (P)-cycling processes were highly enriched after nitrate injection, especially those microorganisms with diverse metabolic capabilities, leading to potential in situ bioremediation of the contaminated sediment, such as PBDE and PAH reduction/degradation. This study provides new insights into our understanding of sediment microbial community responses to nitrate addition, suggesting that indigenous microorganisms could be successfully stimulated for in situ bioremediation of POPs in contaminated sediments with nitrate addition. © 2014 International Society for Microbial Ecology.


PubMed | Yuewei Edible Fungi Technology Co., State Key Laboratory of Applied Microbiology Southern China, Guangzhou University and Sunnybrook Research Institute
Type: | Journal: Oncotarget | Year: 2017

We conducted a study of Ganoderma lucidum metabolites and isolated 35 lanostane-type triterpenoids, including 5 new ganoderols (1-5). By spectroscopy, we compared the structures of these compounds with known related compounds in this group. All of the isolated compounds were assayed for their effect against the human breast carcinoma cell line MDA-MB-231 and hepatocellular carcinoma cell line HepG2. Corresponding three-dimensional quantitative structure-activity relationship (3D-QSAR) models were built and analyzed using Discovery Studio. These results provide further evidence for anti-cancer constituents within Ganoderma lucidum, and may provide a theoretical foundation for designing novel therapeutic compounds.


PubMed | Guangdong Institute of Microbiology and State Key Laboratory of Applied Microbiology Southern China
Type: | Journal: Scientific reports | Year: 2015

Viability is a common issue of concern in almost all microbial processes. Fluorescence-based assays are extensively used in microbial viability assessment, especially for mixed-species samples or biofilms. Propidium iodide (PI) is the most frequently used fluorescence indicator for cell viability based on the membrane permeability. Our results showed that the accumulation of succinate from fumarate respiration could induce PI-permeability in Shewanella decolorationis biofilm cells. Confocal laser scanning microscope further showed that the PI-permeable membrane could be repaired in situ when the extracellular succinate was eliminated by switching fumarate respiration to electrode respiration. Simultaneously with the membrane repair, the electrode respiring capacity of the originally PI-permeable cells was recovered. Agar-colony counts suggested that a major portion of the repaired cells were viable but nonculturable (VBNC). The results evidenced that S. decolorationis S12 has the capacity to repair PI-permeable membranes which suggests a reevaluation of the fate and function of the PI-permeable bacteria and expanded our knowledge on the flexibility of bacterial survival status in harsh environments.

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