State Key Laboratory of Applied Microbiology south China

Guangzhou, China

State Key Laboratory of Applied Microbiology south China

Guangzhou, China
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Li H.,Sun Yat Sen University | Qi T.,Sun Yat Sen University | Huang Z.-S.,Sun Yat Sen University | Ying Y.,Shenzhen University | And 6 more authors.
Journal of Huazhong University of Science and Technology - Medical Science | Year: 2017

In order to investigate the relationship between gut microbiota and type 2 diabetic erectile dysfunction (T2DED), we analyzed the characteristics of gut microbiota in the Sprague-Dawley (SD) rats with T2DED. Thirty-five SD rats were randomly divided into two groups: control group (n=15) with normal diet, and experimental group (n=20) with construction of T2D model. Faecal and serum samples were collected at 2nd and 8th week after establishment of T2D model, respectively. Faecal samples were used for analysis of gut microbiota, and serum samples for detection of trimethylamine N-oxide (TMAO), lipopolysaccharide (LPS), and inflammatory factors like interleukin-1 (IL-1), IL-2, IL-10, and monocyte chemoattractantprotein-1 (MCP-1). The main compositions of gut microbiota were Bacteroidetes, Proteobacteria and Firmicutes at the phylum level, and Oscillospira, Allobaculum, Bacteroides, Ruminococcus, SMB53, Prevotella, Coprococcus, Sutterella and Blautia at the genus level with relatively higher abundance in all SD rats. The relative abundance of Enterococcus, Corynebacterium, Aerococcus, Facklamia (opportunistic pathogens in most case) increased, and that of Allobaculum, Bifidobacterium, Eubacterium, Anaerotruncus (beneficial bacteria) decreased in T2DED group as compared with that at 2nd week after establishment of T2D model (T2D2 group). The serum contents of TMAO, LPS, IL-1, IL-2, IL-10 and MCP-1 in T2DED group were significantly higher than those in control group. The gut microbiota of T2DED rats was inhibited. The gut microbiota of T2DED rats had changed, as the relative abundance of beneficial bacterium was decreased while that of opportunistic pathogens was increased. The variations of gut microbiota might lead to inflammation and prompt the emergence of erectile dysfunction in the rats with T2D. TMAO might play an important role in the formation of T2DED. © 2017, Huazhong University of Science and Technology and Springer-Verlag GmbH Germany.


Zeng P.,Guangdong Institute of Microbiology | Zeng P.,Guangdong Open Laboratory of Applied Microbiology | Zeng P.,State Key Laboratory of Applied Microbiology South China | Li J.,Guangdong Institute of Microbiology | And 14 more authors.
Environmental Technology (United Kingdom) | Year: 2016

A pilot-scale biotrickling filter (BTF) was established in treating the waste gases that are intermittently produced from an automobile paint-manufacturing workshop. Results showed that the BTF required longer time to adapt to the aromatic compounds. The removal efficiencies (REs) for all aliphatic compounds reached more than 95% on day 80. Aromatic compounds were not easily removed by the BTF. The REs obtained by the BTF for toluene, ethylbenzene, m-xylene, o-xylene and p-xylene on day 80 were 72.7%, 77.2%, 71.9%, 74.8% and 60.0%, respectively. A maximum elimination capacity (EC) of 13.8 g-C m-3 h-1 of the BTF was achieved at an inlet loading rate of 19.4 g-C m-3 h-1 with an RE of 72%. Glucose addition promoted the biomass accumulation despite the fact that temporal decrease of REs for aromatic compounds occurred. When the inlet loading rates exceed 11.1 g-C m-3 h-1, the REs of the aromatic compounds decreased by 10% to 15%. This negative effect of shock loads on the performance of the BTF can be attenuated by the pre-treatment of the photocatalytic reactor. Nearly all components were removed by the combined system with REs of 99%. © 2015 Taylor & Francis.


Liao D.,South China University of Technology | Liao D.,Guangdong Institute of Microbiology | Liao D.,State Key Laboratory of Applied Microbiology south China | Liao D.,Guangdong Open Laboratory of Applied Microbiology | And 13 more authors.
Biotechnology and Bioprocess Engineering | Year: 2015

A pilot-scale biotrickling filter (BTF) was designed to treat volatile organic compounds (VOCs) emitted from a typical waste printed circuit board (WPCB) pyrolysis workshop. Measured by gas chromatography-mass spectrometry (GC-MS), the main components of VOCs and their concentrations were benzene, toluene, chlorobenzene, ethyl-benzene, xylene, styrene, benzaldehyde, and trimethyl-benzene. The removal efficiencies of the BTF for these compounds ranged from 81.1 to 97.8% after 90 days of operation. The maximum elimination capacity of 25.94 g/m3 h was obtained with the inlet loading of 30.72 g/m3 fixed the fixed empty-bed residence time (EBRT) of 9.80 sec. Hazard ratio index based on threshold limit value for time weighted average (TLV-TWA) and VOCs concentrations indicated that the cancer risk of VOCs was significantly reduced after the BTF treatment. The microbial community analysis revealed initial inoculum and some emerging bacteria played crucial roles in the improvement of BTF performance with the biodegradation of this kind of VOCs by the polymerase chain reactiondenaturing gradient gel electrophoresis (PCR-DGGE) technique and pyrosequencing analyses indicated that proteobacteria phylum was the dominant in the BTF. All above results indicated that VOCs with multicomponent and fluctuant concentrations from a typical waste printed circuit board pyrolysis workshop were removed efficiently and in an environmentally friendly way by the biofiltration method. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.

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