State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base

Guangzhou, China

State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base

Guangzhou, China

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Zheng Y.-Y.,Guangdong University of Technology | Zheng Y.-Y.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Wu Q.-P.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Wu K.-G.,Guangdong University of Technology | And 3 more authors.
Modern Food Science and Technology | Year: 2013

To establish an ERIC-PCR molecular typing method for Campylobacter jejuni genotyping, the subtyping effect of ERIC-PCR and biochemical typing methods were compared. L16(54) orthogonal text was designed with four factors of Mg2+, dNTPs, primers, TaqDNA polymerase concentration to explore the suitable reaction conditions in a wide range. Then three factors (except the primers), template concentration and annealing temperature were further optimized in a small range for final optimization. The optimized ERIC-PCR method was used to type the 24 C. jejuni isolates. According to the results of biochemical reactions to biochemical type the strains, the ERIC-PCR molecular typing method was compared with biochemical typing method. C. jejuni strains were grouped into 22 types. The ERIC-PCR method exhibited better disciminative results in molecular typing with discrimination index of 0.92. The C. jejuni isolates were classified to 19 biochemical types by biochemical typing method, which showed the diversity of genes in the strains. The ERIC-PCR subtyping method showed the genetic diversity of the strains better than biochemical typing method, which was an efficient method for typing and tracking analyses. It was suitable for genetic diversity analysis of of C. jejuni strains.


Li J.,South China University of Technology | Li J.,Guangdong Institute of Microbiology | Li J.,Guangdong Open Laboratory of Applied Microbiology | Li J.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | And 10 more authors.
Applied Microbiology and Biotechnology | Year: 2012

Two identical biotrickling filters named BTFa and BTFb were run in parallel to examine their performances in removing hydrogen sulfide. BTFa was filled with ceramic granules, and BTFb was filled with volcanic rocks. The results showed that BTFb was more robust than BTFa under acidic conditions. At empty bed residence times (EBRTs) of 20 and 15 s, the removal efficiency of BTFa was close to 100%. At EBRTs of 10 and 5 s, the removal efficiency of BTFa slightly decreased. The removal efficiencies of BTFa decreased by different degrees at the end of each stage, dropping to 94%, 81%, 60%, and 71%, respectively. However, the H2S removal efficiency in BTFb consistently reached 99% throughout the experiment. Pyrosequencing analyses indicated that members of Thiomonas dominated in both BTFs, but the relative abundance of Acidithiobacillus was higher in BTFb than in BTFa. © 2011 Springer-Verlag.


Pan T.,South China University of Technology | Pan T.,Guangdong Institute of Microbiology | Pan T.,Guangdong Open Laboratory of Applied Microbiology | Pan T.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | And 12 more authors.
Separation Science and Technology (Philadelphia) | Year: 2013

A method for removing four triphenylmethane dyes from wastewater by cloud point extraction with the nonionic surfactant Triton X-114 (TX-114) was developed. The triphenylmethane dyes were crystal violet, ethyl violet, malachite green and brilliant green. The cloud point of TX-114 generally increased in the presence of any of the four dyes. In the cloud point system, these dyes were solubilized into a coacervate phase that left a color-free dilute phase. The extraction efficiency of the dyes increased with the temperature, TX-114 concentration, and salt (NaCl and CaCl2) concentration. More than 97% TX-114 in the dilute phase was recovered by adjusting the volume ratio of dichloromethane to the dilute phase. The Langmuir-type adsorption isotherm was used to describe the dye solubilization. The Langmuir constants m and n were calculated as functions of temperature. The results showed that the solubilization of the triphenylmethane dyes in the cloud point system was related to the partition coefficient and their molecular structures. © 2013 Copyright Taylor and Francis Group, LLC.


Deng D.,Ocean University of China | Mei C.,Guangdong Institute of Microbiology | Mei C.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Mai K.,Ocean University of China | And 3 more authors.
Aquaculture Research | Year: 2013

A 60 days feeding experiment was conducted to determine the effects of YK-6 (a yeast culture feed supplement) on growth and immune responses of white shrimp, Litopenaeus vannamei and aquaculture environment in commercial scale farms composed of nine ponds. Three treatments (control, Diet 1 and Diet 2) were designed to contain YK-6 levels of 0.0, 1.0 and 1.5 g kg-1 respectively. At the end of the test, the mean production of Diet 1 (0.649 ± 0.030 kg m-2) and Diet 2 (0.648 ± 0.033 kg m-2) were significantly higher than that of the control by 29.5% and 29.3% respectively. The feed conversion ratio decreased significantly by 12.3% (Diet 1) and 8.5% (Diet 2) compared with the control group respectively. The environmental indices indicate that the quality of water and pond sediment was improved. The endotoxin concentrations, the number of Vibrios and heterotrophic bacteria in the shrimp intestine of the treatment groups were significantly lower than that of the control, particularly in the later stage of the farming period. The YK-6 extended positive effects on growth, improved sediment quality, reduced endotoxin in shrimp intestine, and enhanced activities of lysozyme and phenoloxidase. © 2012 John Wiley & Sons Ltd.


Yang Y.,Guangdong Institute of Microbiology | Yang Y.,Guangdong Academy of science | Guo J.,Guangdong Academy of science | Sun G.,Guangdong Institute of Microbiology | And 5 more authors.
Bioresource Technology | Year: 2013

Microbial electrochemical snorkel (MES) reactor is a simplified bioreactor based on microbial fuel cells (MFCs) and has been suggested to be a promising approach to solve many environmental problems. However, the microbial processes in MES reactors have not yet been characterized. This study shows that Shewanella decolorationis S12 can use the conductive snorkel as direct electron acceptor for respiration and growth. Similar with current-generating biofilms, cellular viability in MES biofilms decreased with the distance from snorkel. MES reactors showed more rapid cell growth and substrate consumption than MFCs. Although the biomass density of MES biofilm was higher than that of anode biofilms, the current-generating capacity and electrochemical activity of MES biofilm were lower, which could be attributed to the lower cytochrome c expression in MES biofilm caused by the higher redox potential of MES. These microbiological and electrochemical properties are essential for the further development of MES reactors. © 2012 Elsevier Ltd.


Yang X.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Wu Q.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Zhang J.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Dong X.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Liu S.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base
Wei sheng yan jiu = Journal of hygiene research | Year: 2013

OBJECTIVE: Salmonella isolates from meat in Guangdong Province were characterized to determine their phenotype and serotype, to investigate contamination status of meats by Salmonella and provide scientific basis for detection and further control.METHODS: 209 meat samples were detected according to methods GB 4789.4--2010 National food safety standard Food microbiological examination: Salmonella. The API 20E system was used to identify isolates. Serotyping of Salmonella isolates was performed by slide agglutination method using antisera to Salmonella.RESULTS: According to the detection results of 209 meat samples, forty-six Salmonella strains were isolated from 42 meat samples, the positive rate was 20. 10%. The majority of positive samples were fresh meat, 69.23% in duck, 37.14% in chicken, 20.00% in beef and 16.67% in pock. The strains identified as Salmonella by the API 20E were distributed in 7 profile numbers with two of them being predominant (6704752 and 6704552). The most common serotypes were Derby (21.74%), Typhimurium (10.87%), Enteritidis (8.70%), Tshiongwe (8.70%), Indiana (8.70%), Weltevreden (8.70%).CONCLUSION: Salmonella present in retail meats were common and phenotypically diverse in Guangdong Province.


Yang Y.,Guangdong Institute of Microbiology | Yang Y.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Yang Y.,Guangdong Open Laboratory of Applied Microbiology | Xu M.,Guangdong Institute of Microbiology | And 8 more authors.
Process Biochemistry | Year: 2012

Bioelectrochemical systems (BES), typically microbial fuel cells (MFCs), have attracted increasing attention in the past decade due to their promising applications in many fields, such as bioremediation, energy generation and biosynthesis. Current-generating microorganisms play a key role in BES. The process of transferring electrons to electrode has been considered as a novel anaerobic bacteria respiration, and more and more bacteria capable of exchanging electrons with electrodes have been isolated. Among those bacteria, Shewanella and Geobacter genera are the most frequently used model organisms in the studies of BES, as well as the bacteria-electrode electron transfer mechanisms. Many significant new findings in the field of the bacterial extracellular electron transfer in BES have been reported recently. A better understanding of the mechanisms of bacterial extracellular electron transfer would provide more efficient strategies to enhance the applicability of BES. This review summarizes the recent advances of extracellular electron transfer mechanisms with foci on Shewanella and Geobacter species in BES. © 2012 Elsevier Ltd.


PubMed | State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base
Type: Journal Article | Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2012

To investigate the chemical constituents of an endophytic fungus, Nodulisporium sp. A4, from the medicinal plant Aquilaria sinensis and search for antitumor natural products.The fungus was cultured in liquid medium and extracted with EtOAc. The compounds were isolated by various chromatographic methods (silica gel, reverse silica gel, Sephadex-LH20, preparative TLC and so on) and recrystallization. Structural elucidation was conducted by extensive analysis of spectroscopic data as well as by comparison with literature reports. The antitumor activity of isolated compounds was tested by MTT method in vitro.Seven compounds were isolated and identified from the broth culture, their structures were determined to be 5-methyl-2-vinyltetrahydrofuran-3-ol (1), 6-methyl-2-(5-methyl-5-vinyltetrahydrofuran-2-yl) hept-5-en-2-ol (2), 6alpha-hydroxycyclonerolidol (3), rel-(1S,4S, 5R,7R,10R)-10-desmethyl-1-methyl-11-eudesmene (4), tyrosol (5), 8-methoxynaphthalen-1-ol (6), and 1,8-dimethoxynaphthalene (7). Three compounds were isolated and identified from the mycelia as ergosterol (8), ergosterol peroxide (9), and cerevisterol (10). The in vitro pharmalogical evaluation results displayed that compounds 3 and 4 showed 89.1%, 44.2% and 82.3%, 79.8% inhibition against tumor cell lines SF268 and NCI-H460 at 100 mg x L(-1), respectively.Compound 1 was a new natural product, compounds 2, 3, 7 and 10 were reported from the genus Nodulisporium sp. for the first time. Compounds 3 and 4 exhibited weak inhibitory effects on the proliferation of tumor cell lines SF268 and NCI-H460.


PubMed | State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base
Type: Journal Article | Journal: Biodegradation | Year: 2012

Polybrominated diphenyl ethers (PBDEs) are a class of widely used flame retardants that have been highly accumulated in sediments. It is reported that microorganisms play an important role in the reductive debromination of PBDEs in anaerobic sediments. However, little is known about the effects of electron donors on the microbial community structure and their debromination capacity in PBDE transformation. In this study, alternate carbon substrates were used as electron donors to enrich the PBDE-debrominating microbial consortia to evaluate the effects of electron donors on PBDE microbial debromination. Decabromodiphenyl ether (BDE-209) was found to be the dominant (more than 50%) PBDEs congener in all consortia, and the percentage of BDE-209 was deceased by 12% (methanol), 11% (ethanol), 8% (acetate), 9% (lactate), 5% (pyruvate), and 11% (no electron donors), while the relative abundances of most lesser-brominated PBDEs increased after 90-day incubation compared to the initial profile of PBDEs. Substantial shifts in the microbial community structure among different amendments were observed based on denaturing gradient gel electrophoresis results. Pseudomonas spp. were identified to be the predominant organisms and the abundances of Band R, which was associated with Pseudomonas sp. SCSWA09, was well correlated with the biodegradation rate of BDE-209. Finally, the microbial community structure was highly correlated with the concentration of deca-BDE, octa-BDE and total nitrogen. These results provide insights into in situ bioremediation of environments contaminated by PBDEs and our understanding of microbial ecology associated with PBDE-debromination.


PubMed | State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base
Type: Journal Article | Journal: Wei sheng yan jiu = Journal of hygiene research | Year: 2013

Salmonella isolates from meat in Guangdong Province were characterized to determine their phenotype and serotype, to investigate contamination status of meats by Salmonella and provide scientific basis for detection and further control.209 meat samples were detected according to methods GB 4789.4--2010 National food safety standard Food microbiological examination: Salmonella. The API 20E system was used to identify isolates. Serotyping of Salmonella isolates was performed by slide agglutination method using antisera to Salmonella.According to the detection results of 209 meat samples, forty-six Salmonella strains were isolated from 42 meat samples, the positive rate was 20. 10%. The majority of positive samples were fresh meat, 69.23% in duck, 37.14% in chicken, 20.00% in beef and 16.67% in pock. The strains identified as Salmonella by the API 20E were distributed in 7 profile numbers with two of them being predominant (6704752 and 6704552). The most common serotypes were Derby (21.74%), Typhimurium (10.87%), Enteritidis (8.70%), Tshiongwe (8.70%), Indiana (8.70%), Weltevreden (8.70%).Salmonella present in retail meats were common and phenotypically diverse in Guangdong Province.

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