State Key Laboratory of Analytical Chemistry for Life Science

Nanjing, China

State Key Laboratory of Analytical Chemistry for Life Science

Nanjing, China

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Ding Y.,State Key Laboratory of Analytical Chemistry for Life Science | Li Y.,Nanjing University | Li Y.,Nanjing University of Information Science and Technology | Qin M.,Nanjing University | And 2 more authors.
Langmuir | Year: 2013

Peptide-based supramolecular hydrogels have been extensively explored in biomaterials owing to their unique bioactive, stimulus-responsive, and biocompatible features. However, peptide-based hydrogels often have low mechanical stability with storage moduli of 10-1000 Pa. They are susceptible to mechanical destruction and solvent erosion, greatly hindering their practical application. Here, we present a photo-cross-linking strategy to enhance the mechanical stability of a peptide-based hydrogel by 104-fold with a storage modulus of ∼100 kPa, which is one of the highest reported so far for hydrogels made of small peptide molecules. This method is based on the ruthenium-complex-catalyzed conversion of tyrosine to dityrosine upon light irradiation. The reinforcement of the hydrogel through photo-cross-linking can be achieved within 2 min thanks to the fast reaction kinetics. The enhancement of the mechanical stability was due to the formation of a densely entangled fibrous network of peptide dimers through a dityrosine linkage. We showed that in order to implement this method successfully, the peptide sequence should be rationally designed to avoid the cross talk between self-assembly and cross-linking. This method is convenient and versatile for the enhancement of the mechanical stability of tyrosine-containing peptide-based hydrogels. We anticipate that the photo-cross-linked supramolecular hydrogels with much improved mechanical stability will find broad applications in tissue engineering and drug controlled release. © 2013 American Chemical Society.


Lin Y.,Anhui Polytechnic University | Lin Y.,Nanjing University | Ding Y.,Nanjing University | Ding Y.,State Key Laboratory of Analytical Chemistry for Life science | Jiang X.,Nanjing University
Progress in Chemistry | Year: 2014

Polymer-inorganic hybrid nanospheres have attracted increasing attention in recent years because of the synergic properties arising from both the polymeric nanospheres and inorganic nanomaterials. Especially, the nanospheres composed by the polymers that have desirable plasticity and biocompatibility, and the inorganic materials with unique optical, magnetic and electric properties are greatly useful in diagnosis and therapy disease. The combinations of functional polymers, inorganic nanomaterials and bioactive molecules can offer synergetic multifunctional nanomedical platforms, which make it possible to accomplish multimodal imaging and monitoring therapy. This article provides a review on the synthetic methodologies for building hybrid nanospheres, and their applications in targeted drug delivery, bio-imaging, cell separation, biosensing and hyperthermia. Perspective and challenges in nanomedical fields are discussed to provide the reference information for development of novel theranostic hybrid nanospheres.


Jun G.,State Key Laboratory of Analytical Chemistry for Life Science | Jun G.,Nanjing University | Jun G.,Jiangsu Institute of Education | Liping J.,State Key Laboratory of Analytical Chemistry for Life Science | And 3 more authors.
Science China Chemistry | Year: 2012

A clear understanding of the nucleation, growth, coarsening, and aggregation processes of nanomaterials is necessary to enable the preparation of highly controlled nanostructures. Among wet chemical synthetic methods, ultrasound-assisted preparation has become an important tool in material science. The formation and crystal growth mechanism under ultrasound is special compared with other wet chemical synthetic routes. In this review, we discussed the chemical and physical effect of ultrasound and summarized the ultrasonic effect on crystallization. The sonolysis of water and the cavitation-induced microjet impact and shockwave are the two key factors in the sonochemical formation of inorganic nanomaterials. The ultrasonic-assisted Ostwald ripening and oriented attachment processes have been reviewed for the possible crystal growth mechanisms in the fabrication of inorganic nanostructures. © Science China Press and Springer-Verlag Berlin Heidelberg 2012.


Xu S.,Nanjing University | Zou B.,Nanjing University | Xiang Z.,China Pharmaceutical University | Miao M.,Nanjing Medical University | And 5 more authors.
Analytical Methods | Year: 2016

Non-invasive detection of trisomy 21 is a safe and effective way for prenatal diagnosis. Although using next generation sequencing technology can achieve non-invasive detection of chromosomal abnormalities, a more convenient and cost-effective method is preferable for routine clinical applications. Here, we proposed a novel method for the detection of trisomy 21 by accurately quantifying the slightly increased amount of chromosome 21 in cell-free DNA from maternal plasma using digital PCR. The segmental duplication fragments on chromosome 21 and chromosome 1 were employed as the detection target of digital PCR. As low as 10% cell-free fetal DNA of trisomy 21 fetus in maternal cell-free DNA was successfully detected. Three trisomy 21 samples were unambiguously picked up from 15 clinical samples, indicating that our method has the potential for non-invasive diagnosis of trisomy 21. © The Royal Society of Chemistry 2016.


Huang Z.,State Key Laboratory of Pharmaceutical Biotechnology | Wang Z.,State Key Laboratory of Pharmaceutical Biotechnology | Long S.,State Key Laboratory of Pharmaceutical Biotechnology | Jiang H.,State Key Laboratory of Pharmaceutical Biotechnology | And 5 more authors.
Molecular Pharmaceutics | Year: 2014

Functional engineered nanoparticles are promising drug delivery carriers. As the construction of a functional nanocarrier always needs the optimization of multiple technical variables, efficient in vitro high-throughput evaluation methods would help to shorten the development cycle. In the present study, we generated a tissue mimic of the colon of inflammatory bowel disease (IBD) patients. Generally, Caco-2 cells and THP-1 cells were grown in a 3-D matrix with different number, spatial distribution and specific extracellular cell matrix (ECM) composition according to real healthy and inflamed animal colon tissues. After interlerukin-1β/lipopolysaccharide (LPS) stimulation, the artificial model closely resembled the pathological features of IBD patients colon, including massive cytokines and mucus production, epithelium defect and leukocytic infiltration. The tissue and cellular uptake of three different nanoparticles in the artificial model was similar to that in 2,4,6-trinitrobenzenesulfonic acid (TNBS) colitic mice. Most importantly, our artificial tissue can be placed into 96-well plates for high-throughput screening of drug delivery carriers for the treatment of IBD. Our study suggested a readily achievable way to improve current methodologies for the development of colon targeted drug delivery systems. © 2014 American Chemical Society.


Wang H.,Center for Public Health Research | Chang L.,Center for Public Health Research | Wang X.,Center for Public Health Research | Wang X.,Nanjing Medical University | And 10 more authors.
Biochemical and Biophysical Research Communications | Year: 2016

As a cytoplasmic parasite, RNA virus develops sophisticated mechanisms to counter host defense and utilize host proteins to facilitate its replication. Here we found Moloney leukemia virus 10 (MOV10), a highly conserved cellular protein belonging to SF1 helicase family, played critical roles in EV71 infection. Silencing cellular MOV10 could restrict EV71 replication, while over-expressing MOV10 resulted in increased viral replication at low dosage and repressed viral replication at high dosage. Further investigation showed that MOV10 exhibited dual functions in EV71 regulation, its C-terminus positively regulated viral replication by binding to EV71 cloverleaf-like structure and the internal ribosome entry site while the N-terminus showed a potential antiviral activity when individually overexpressed. In addition, RNA-dependent interaction between MOV10 and HuR as well as the co-localization of MOV10 and processing bodies were also observed post infection. Taken together, our data indicate a crucial role of MOV10 in EV71 infection for the first time, providing new insights for its roles in EV71 infection. © 2016 Elsevier Inc.


Zhang J.,State Key Laboratory of Analytical Chemistry for Life Science | Zhao Y.,State Key Laboratory of Analytical Chemistry for Life Science | Yuan C.-G.,State Key Laboratory of Analytical Chemistry for Life Science | Ji L.-N.,Nanjing University | And 4 more authors.
Langmuir | Year: 2014

The Donnan potential is successfully isolated from ion pair potential on a ferrocene-labeled polyelectrolyte (DNA) monolayer. The isolated Donnan potential shifts negatively upon the increase in NaClO4 concentration with a slope of -58.8 mV/decade. With the salt concentration grown up to 1 M, the stretched DNA chains in low salt concentration are found to experience a gradual conformation relaxing process. At salt concentrations higher than 2 M, Donnan breakdown occurs where only the ion pair effect modulates the apparent potential. The apparent formal potential also shows strong dependence on solution pH, which reveals that the charge density in the polyelectrolyte monolayer plays an important role in the establishment of Donnan equilibrium. © 2014 American Chemical Society.


Wo X.,State Key Laboratory of Analytical Chemistry for Life Science | Luo Y.,State Key Laboratory of Analytical Chemistry for Life Science | Tao N.,State Key Laboratory of Analytical Chemistry for Life Science | Wang W.,State Key Laboratory of Analytical Chemistry for Life Science | Chen H.-Y.,State Key Laboratory of Analytical Chemistry for Life Science
Science China Chemistry | Year: 2016

Molar concentration of gold nanoparticles is one of the most critical parameters of gold colloids in order to develop their applications in sensing, diagnostics and nanomedicine. Previous methods often stand just for gold nanoparticles with regular shape and narrow size distribution. In the present work, we proposed an absolute quantification method that determined the molar concentration of gold nanoparticles with arbitrary shapes and polydisperse sizes. This approach involved the real time monitoring and counting of individual nanoparticles collision events, from which the quantification of molar concentration was achieved using a theoretical model consisting of Fick’s laws of diffusion and Stokes-Einstein equation. The determination of spherical gold nanoparticles concentration resulted in excellent agreement with traditional spectrometry method. It was further demonstrated that the present approach can be expanded to determine the molar concentration of gold nanoparticles with arbitrary shapes and poly-diversed distributions. © 2016, Science China Press and Springer-Verlag Berlin Heidelberg.


Fang H.,State Key Laboratory of Analytical Chemistry for Life Science
Lecture Notes in Engineering and Computer Science | Year: 2013

In order to fabricate high speed and low cost ADC, a new type Analog-to-Digital converter (ADC) was presented on the basis of the periodicity of trigonometric function. This kind of ADC is defined as T-ADC. Each bit conversion of T-ADC is performed nearly in parallel. An N-bit T-ADC consists of a trigonometric function circuit, N 1 groups of analog multipliers and operational amplifiers, N comparators, and a decoder. The input voltage is mapped to cosine of the corresponding angle first. Then the angle in cosine function is doubled N -1 times. All the cosine function voltages of the doubled angles are used to generate the result of A/D conversion. Theoretically, T-ADC can achieve the conversion speed of flash ADC. In this paper, the sources of error in T-ADC and their influences on T-ADC are analyzed. Based on it, an error reduction approach in T-ADC is presented.

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