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Yang H.-F.,University of Science and Technology Beijing | Yang H.-F.,State Key Laboratory for High Efficiency Mining and Safety of Metal Mines | Li T.,University of Science and Technology Beijing | Li T.,State Key Laboratory for High Efficiency Mining and Safety of Metal Mines | And 6 more authors.
International Journal of Minerals, Metallurgy and Materials | Year: 2014

In this study, we characterized strain F9 and evaluated the interaction between strain F9 and hematite by scanning electron microscopy (SEM), Fourier transform infrared spectrophotometry (FTIR), zeta potential, flotation, and other methods. The results showed that strain F9 belongs to Serratia marcescens. This brevibacterium had CH2, CH3, and hydroxyl groups on its cell wall, which imparted a strong hydrophobic and negative charge. Adsorption of strain F9 reduced the zeta potential of the hematite surface and increased the hydrophobicity of the hematite surface, thereby generating hydrophobic hematite agglomerates. At least four groups on strain F9 interacted with the hematite surface, which contributed to chemical interactions of carboxylic groups and hydrophobic association among hydrophobic hematite particles. The possible use of strain F9 as a bio-collector for hematite flotation was proved. © 2014 University of Science and Technology Beijing and Springer-Verlag Berlin Heidelberg.


Yang H.,State Key Laboratory for High Efficiency Mining and Safety of Metal Mines | Yang H.,University of Science and Technology Beijing | Li T.,State Key Laboratory for High Efficiency Mining and Safety of Metal Mines | Li T.,University of Science and Technology Beijing | And 6 more authors.
International Journal of Mineral Processing | Year: 2013

A microbial bio-based collector was isolated from iron mine soil using a combination of flotation and culturing techniques. Four microbial strains with greater than 75% recovery of pure hematite were separated using the technique. Classification and identification of the 4 strains by 16S rDNA sequences were conducted. Among them only 1 strain, termed F3, can act as a collector for hematite, as it is not pathogenic to humans. The morphology and hematite adsorption (by scanning electron microscopy; SEM), composition, zeta potential and hydrophobicity of strain F3 were further evaluated. Based on the changes in the zeta potential and hydrophobicity of a hematite surface before and after adsorption with F3, its adsorption mechanism was also analysed. The results revealed that the combination technique of flotation and bacterial culturing is a highly effective technique for isolating bio-collectors from iron mine soil. Two flotation stages were required to isolate strain F3, which belongs to the genus Stenotrophomonas. This rod-shaped bacterium had CH2, CH 3 and phosphate groups on its cell wall that imparted strong hydrophobicity and negative charge. Adsorption of strain F3 reduced the zeta potential of a hematite surface and enhanced its hydrophobicity. The adsorption of F3 to hematite is presumed to be through chemical adsorption. Therefore, strain F3 can be used as a bio-collector for hematite flotation. © 2013 Elsevier B.V.

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